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Yasumasa Kuwahara,Naoki Mori,Kiminori Shimizu,Chihiro Tanaka,Mitusya Tsuda Korean Society of Applied Entomology 1998 Journal of Asia-Pacific Entomology Vol.1 No.1
Female sex pheromones, which elicit mounting behavior of conspecific males, were identified in 4 species of the genus Caloglyphus(Acarina: Acaridae). Although the sex pheromones are distributed over both males and femles, they could be classified into two groups; one is an adult-specific pheromone, and the other is one that is detectable even in nymphal stages. In addition, in the adult-specific species, the pheromonal content ratios of female to male were found to be larger than those observed in the nonspecific species. The two types of pheromonal distribution were compared with phylogenetic relationships based on a portion of the mitochondrial cytochrome oxidase subunit I(COI) gene in the four species. The polymerase chain reaction was used to amplify the COI from the four mites, with primers used in spider mites(Tetranychidae). Fragments of the COI(453bp) were obtained for the three species. The phylogenetic tree, based on the COI sequences, was inferred using a maximum parsimony method. Analysis of the pheromonal distribution within this phylogenetic framework agreed with the hypothesis that the mite sex pheromone evolved from a common compound, which originally fundtioned as a male sexual excitant, into a biologically active, sex linked specific one.
Jay Kant Raut,Akira Suzuki,Toshimitsu Fukiharu,Kiminori Shimizu,Chihiro Tanaka,Shogo Takeshige 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4
Addition of ammonia or any nitrogenous materials to the soil that release ammonia causing alkaline condition during decomposition stimulates the fruiting of a particular chemoecological group of fungi, called ammonia fungi (Sagara 1975). The study of ammonia fungi by artificial application of urea in forest soil has been done in diverse geographical regions such as in Japan, Taiwan, New Zealand, Western Australia, and UK. Up to date about 70 species of ammonia fungi have been recorded in those regions. However, ammonia fungi in the boreal forest of American continent have not yet been investigated. Thus, we collected the soils of A0 and the upper layer of HA horizons in plant pots from aspen forest near Edmonton, Canada. Thereafter, we applied urea (granular fertilizer; 46% nitrogen, 10 mg/g dry soil) in plant pots and incubated at 25˚C under 12 hours dark and light regime. After 40 days of incubation, several basidiomata of Coprinopsis species appeared. Among them one specimen was identified as C. rugosobispora based on macro- and microscopic features. Morphologically this species was very similar to C. phlyctidospora which was characterized by warty, ovoid basidiospores, and diverticulate veil elements. C. phlyctidospora has 4-spored basidia while C. rugosobispora had only 2-spored. In the beginning, it was thought probably it was only a 2-spored form of C. phlyctidospora. The basidiospore of C. rugosobispora (9.8-11.7×8.3-9.6㎛) was distinctly larger than that of C. phlyctidospora (8.4-10.6×6.0-7.6 ㎛). It was therefore separated from the C. phlyctidospora. Furthermore in this study we investigated its phylogenetic relationship based on the nuclear rDNA sequence in ITS regions and mating reactions among its close allies and further confirmed it as a distinct species. This is the first record of C. rugosobispora from American continent since it has been collected only from Europe (Belgium and Netherlands). Although urea effectively stimulated its occurrence but it has not yet been reported any other urea application studies so far. This indicates it is a new record in ammonia fungi as well.
Metabolic Disorders : Adiposcience-based approach toward novel therapies for the metabolic syndrome
( Hiroaki Masuzaki ),( Tomohiro Tanaka ),( Shintaro Yasue ),( Takako Ishii ),( Sadanori Okada ),( Ken Ebihara ),( Kiminori Hosoda ),( Kazuwa Nakao ) 한국생화학분자생물학회 (구 한국생화학회) 2008 생화학분자생물학회 춘계학술발표논문집 Vol.2008 No.-