심한 골병변으로 발현된 기능성 낭종성 부갑상선 선종

전숙,김영희,박지영,고관표,박철영,김덕윤,우정택,김성운,김진우,김영설,고석환 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.2

낭종성 부갑상선 선종과 심한 골병변을 동반한 부갑상선 기능항진증은 매우 드문 질환으로서, 저자들은 양측 고관절의 통증을 초기 주소로 내원한 환자에서 고칼슘혈증과 부갑상선 호르몬 증가, 골병변의 방사선적 소견을 통해 부갑상선 기능항진증을 진단하고, 경부 초음파와 컴퓨터 단층 촬영, 부갑상선 스캔검사 및 수술중 부갑상선 낭종액 검사 등을 통해 기능성 부갑상선 낭종의 한 종류인 낭종성 부갑상선 선종을 진단하고 수술적 제거를 통하여 정상화된 1예를 경험하였다. A cystic parathyroid adenoma is rare. A case of primary hyperparathyroidism, with the cystic formation of a parathyroid adenoma and a severe bony lesion, is reported. A 52-year-old male was admitted due to pain in both hips and for evaluation of hypercalcemia. The plasma level of the intact parathyroid hormone (iPTH) was elevated to 1424 pg/mL. Ultrasonography and the computed tomography revealed a parathyroid cyst on the left thyroid lower pole. Parathyroid scintigraphy detected a parathyroid adenoma. A radiograph showed a subperiosteal bone resorption on the phalanges, and a brown tumor (osteitis fibrosa cystica) on the femur shaft was noted. A surgical excision of the parathyroid adenoma was performed. The PTH level in the cystic fluid was increased. A histological examination confirmed a cystic parathyroid adenoma. The PTH level was normalized after the operation (J Kor SOC Endocrinol 18:214-220, 2003).

세미모노코크 구조의 라멘解析

김천욱,전흥재,김지홍 연세대학교 산업기술연구소 1989 논문집 Vol.21 No.1

In this study a semimonocoque element which is composed of rigid frames and a plate is investigated. The structural behavior of the plate subjected to shear is substituted by two equivalent diagonal bars. Applying the equivalent structural elements the semimonocoque structure is formulated into the equivalent rigid frame structure. Numerical analyses of the two types of car-body-structure are performed with the rigid frame program. The numerical results of the present study are compared with those of SAP V-2 program and they are in good agreement within 1% and 2% for the displacements and the bending moments, respectively. The displacements at the load application points and the bending moments of this study agreed with those of experiments in the range of 8% and 7%, respectively.

車體構造解析硏究

김지홍,김천욱,이신영 연세대학교 산업기술연구소 1988 논문집 Vol.20 No.1

An analysis on the load-bearing capacity of floor frame and body structure is investigated by the three dimensional rigid frame analysis. The load-bearing capacity of the body structure is estimated on the basis of comparison to the floor-frame-only structure. The location and magnitude of the maximum displacement are also determined and show good agreement with those of a previous study.In the present study the load-bearing capacity of the floor frame of van-type structures is about 80 percent of the total loadings.

통계적 실험계획에 의한 PECVD로 증착된 실리콘 산화막의 특성 분석

李晟準,金洸範,崔鑛泉,漢秀一,尹知原,金劉邏,朴宰賢,洪尙眞 명지대학교 산업기술연구소 2006 産業技術硏究所論文集 Vol.25 No.-

According as the current needs of low temperature in semiconductor manufacturing process, PECVD using low temperature and high deposition rate is becoming more of a concern. However, PECVD equipment has the defect possibility on the interface between substrates and gate oxide layer, because ions or electrons with much high energy clash the interface. In this study, we embarked on the experiment with Statistical Design of Experiment and then also analyze which parameters influence on the characteristic of silicon dioxide filems. We finally made a constructive proposal for process optimization conditions.

SELDI-TOF MS를 이용한 탄저 치사독소 처리에 의한 생쥐 재식세포주의 단백질체 분석

정경화,서귀문,김성주,김지천,채영규 한양대학교 이학기술연구소 2007 이학기술논문지 Vol.10 No.-

탄저는 탄저균이 동물에 감염되어 발병하는 인수 공통 질병이다. 탄저균의 아포가 감염되면 식균작용에 의해 대식세포 내로 들어가게 된다. 대식세포 내에서 아포는 발아하며 대식세포에서 숙주의 체내로 들어가게 된다. 치사독소는 방어항원과 치사요소로 구성되어있다. 방어항원은 세포의 탄저독소 수용체에 결합하여 방어항원에 결합한 치사요소를 세포내로 운반한다. 운반된 치사요소는 mitogen-activated protein kinase kinase (MAPKK)의 N-말단 부위를 전달하는 것으로 알려졌으나, 이 기작은 세포 사멸에 직접적인 영향을 주지 않는다. 탄저 치사독소에 의한 세포 사멸에 대한 많은 연구가 이루어지고 있으나, 그 기전이 아직까지 명확하게 규명이 되지 않고 있는 실정이다. 본 연구에서는 탄저 치사독소 처리에 의한 생쥐 대식세포주 (RAW 264.7)의 단백질의 발현양상을 조사하여 탄저 치사독소의 세포내 작용기작을 규명하고자 하였다. 단백질 발현 양상을 SELDI-TOF MS을 이용하여 분석하였다. 치사독소를 처리한 대식세포주에서 3,973 Da 단백질의 발현은 90분과 180분에 2배 정도 증가하였다. 단백질 6,066 Da의 발현은 60분에 두 배 증가하고 시간이 지남에 따라 점점 증가하였다. 단백질7,520 Da은 발현이 감소하고, 단백질 8,375 Da과 16,769 Da의 발현은 점점 감소하여 180분에는 절반으로 감소하는 경향을 보였다. MAPKKs가 절단되는 60분이 기준으로 점차 변화가 일어나는 이들 단백질은 치사독소의 절단된 MAPKKs에 의해 발현이 변화하는 것으로 추정되었다. Anthrax is an infectious disease caused by Bacillus anthracis. The spores of B. anthracis are accessed into the body and germinated in macrophages. B. anthracis secretes three virulence factors (such as, protective antigen : PA, lethal factor : LF, and edema factor : EF), and escapes destruction and lyse the macrophages by an unknown mechanism. Anthrax toxins play a central role in pathogenesis of anthrax. Lethal toxin (LeTx) is a mixture of PA and LF. LF is a zinc-dependent endoproteinase that cleaves the amino terminus of mitogen-activated protein kinase kinases (MAPKKs). The role of LeTx in mediating these effects is unknown, largely due to the difficulty in identifying and assigning function to individual proteins. To analyze the cytosolic protein profile of murine macrophages treated with LeTx, we have performed surface enhanced laser desorption/ionization time-of-flight mass spectrometer. The protein peak of 3,973 Da was increased 2 fold at 90 min and 180 min in murine macrophages treated with the LeTx. The protein peak of 6,066 Da was increased 2 fold at 60 min and gradually increased at 90 min and 180min. Protein peak 8,973 Da and 16,769 Da was decreased 2 fold in 180 min. Our results suggest that these proteomic approaches are a useful tool to study gene and protein expression in intoxicated macrophages.

Supplementation of Modified Mannitol-Yolk-Polymyxin B Agar with Cefuroxime for Quantitative Detection of Bacillus cereus in Food

Chon, Jung-Whan,Kim, Young-Ji,Kim, Dong-Hyeon,Song, Kwang-Young,Kim, Hyunsook,Seo, Kun-Ho Institute of Food Technologists 2019 Journal of Food Science Vol.84 No.1

<P> The presence of unwanted competing flora has been the most common confounding factor in the enumeration of <I>Bacillus cereus (B. cereus)</I> using selective media such as mannitol-yolk-polymyxin B agar (MYPA). The objective of this study was to improve MYPA selectivity for <I>B. cereus</I> by supplementation with a second-generation cephalosporin, cefuroxime. The performance of cefuroxime-supplemented MYPA (cefu-MYPA) was evaluated by comparison with original MYPA in 60 food products with established microbiological standards for <I>B. cereus</I> contamination. Cefu-MYPA demonstrated superior recoverability and selectivity for B. cereus compared with original MYPA in most tested foods. <I>B. cereus</I> numbers on MYPA and cefu?MYPA were 363.5 and 462.0 CFU/g, respectively. Competing flora on cefu-MYPA was detected in significantly less samples (70%) compared to original MYPA (93%). In addition, the detection and isolation of suspected colonies were significantly improved in cefu-MYPA because of the reduction or elimination of competing flora in all tested foods except fruit juice, indicating superior selectivity of the modified medium. Our findings suggest that cefuroxime supplementation of MYPA would markedly improve the detection rate of <I>B. cereus</I>, particularly in foods with high levels of indigenous flora. </P>

High Occurrence Rate and Contamination Level of <i>Bacillus cereus</i> in Organic Vegetables on Sale in Retail Markets

Kim, Young-Ji,Kim, Hong-Seok,Kim, Kwang-Yeop,Chon, Jung-Whan,Kim, Dong-Hyeon,Seo, Kun-Ho Mary Ann Liebert, Inc. Publishers 2016 Foodborne Pathogens and Disease Vol.13 No.12

Rapid, Sensitive, and Specific Detection of Salmonella Enteritidis in Contaminated Dairy Foods using Quantum Dot Biolabeling Coupled with Immunomagnetic Separation

Kim, Hong-Seok,Chon, Jung-Whan,Kim, Hyunsook,Kim, Dong-Hyeon,Yim, Jin-Hyuk,Song, Kwang-Young,Kang, Il-Byung,Kim, Young-Ji,Lee, Soo-Kyung,Seo, Kun-Ho Korean Society of Dairy Science and Biotechnology 2015 Journal of Dairy Science and Biotechnology (JMSB) Vol.33 No.4

Colloidal semiconductor CdSe-ZnS core-shell nanocrystal quantum dot (Qdot) are luminescent inorganic fluorophores that show potential to overcome some of the functional limitations encountered with organic dyes in fluorescence labeling applications. Salmonella Enteritidis has emerged as a major cause of human salmonellosis worldwide since the 1980s. A rapid, specific, and sensitive method for the detection of Salmonella Enteritidis was developed using Qdot as a fluorescence marker coupled with immunomagnetic separation. Magnetic beads coated with anti-Salmonella Enteritidis antibodies were employed to selectively capture the target bacteria, and biotin-conjugated anti-Salmonella antibodies were added to form sandwich immune complexes. After magnetic separation, the immune complexes were labeled with Qdot via biotin-streptavidin conjugation, and fluorescence measurement was carried out using a fluorescence measurement system. The detection limit of the Qdot method was a Salmonella Enteritidis concentration of $10^3$ colony-forming units (CFU)/mL, whereas the conventional fluorescein isothiocyanate-based method required over $10^5CFU/mL$. The total detection time was within 2 h. In addition to the potential for general nanotechnology development, these results suggest a new rapid detection method of various pathogenic bacteria from a complex food matrix.

Efficacy of Syringe Filtration for the Selective Isolation of Campylobacter from Chicken Carcass Rinse

Chon, Jung-Whan,Kim, Hong-Seok,Kim, Dong-Hyeon,KIM, Young-Ji,Sung, Kidon,Kim, Hyunsook,Seo, Kun-Ho ational Association for Food Protection 2017 Journal of food protection Vol.80 No.6

<B>ABSTRACT</B><P>We investigated the efficacy of syringe filtration for selective isolation of Campylobacter from chicken carcass rinse by combining syringe filtration with the conventional culture method. Whole chicken carcass rinses were incubated in Bolton enrichment broth, set aside or subjected to syringe filtration, and streaked on Campy-Cefex agar with or without cefoperazone antibiotic supplement. Compared with the conventional method without filtration, 0.65-μm-pore-size syringe filtration resulted in a significantly higher number of Campylobacter-positive samples (23.8 to 37.5% versus 70.0 to 72.5%; P < 0.05), a lower number of plates contaminated with non-Campylobacter (93.8% versus 6.3 to 26.3%), and a lower growth index (1 = growth of a few colonies; 2 = growth of colonies on about half of the plate; and 3 = growth on most of the plate) for competing microbiota (2.9 to 3.0 versus 1.2 to 1.4). When syringe filtration was applied, agar plates containing the antibiotic had significantly less contamination (6.3% versus 26.3%; P &lt; 0.05) and a lower growth index (1.2 versus 1.4) compared with plates without the antibiotic, although the Campylobacter isolation rate was similar (P > 0.05). Syringe filtration combined with conventional enrichment improved the rate and selectivity of Campylobacter isolation from chicken carcasses.</P>

Addition of Rifampicin to Bolton Broth to Inhibit Extended-Spectrum β-Lactamase-Producing Escherichia coli for the Detection of Campylobacter : Modified <i>campylobacter</i> broth with rifampicin…

Chon, Jung-Whan,Kim, Young-Ji,Kim, Young-Jo,Jung, Ji Young,Bae, Dongryeoul,Khan, Saeed,Seo, Kun-Ho,Sung, Kidon Wiley Blackwell (Blackwell Publishing) 2017 Journal of Food Science Vol.82 No.7