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Choi, Hoseong,Kim, Hyun Jung,Lee, Jin Hee,Kim, Ji Soo,Park, Seur Kee,Kim, In Seon,Kim, Young Cheol The Korean Society of Plant Pathology 2012 Plant Pathology Journal Vol.28 No.4
The chitinase producing Lysobacter enzymogenes C-3 has previously been shown to suppress plant pathogens in vitro and in the field, but little is known of the regulation of chitinase production, or its role in antimicrobial activity and biocontrol. In this study, we isolated and characterized chitinase-defective mutants by screening the transposon mutants of L. enzymogenes C-3. These mutations disrupted genes involved in diverse functions: glucose-galactose transpoter (gluP), disulfide bond formation protein B (dsbB), Clp protease (clp), and polyamine synthase (speD). The chitinase production of the SpeD mutant was restored by the addition of exogenous spermidine or spermine to the bacterial cultures. The speD and clp mutants lost in vitro antifungal activities against plant fungal pathogens. However, the gluP and dsbB mutants showed similar antifungal activities to that of the wild-type. The growth of the mutants in nutrient rich conditions containing chitin was similar with that of the wild-type. However, growth of the speD and gluP mutants was defective in chitin minimal medium, but was observed no growth retardation in the clp and dsbB mutant on chitin minimal medium. In this study, we identified the four genes might be involved and play different role in the production of extracellular chitinase and antifungal activity in L. enzymogenes C-3.
Two-photon microscopy using an Yb(3+)-doped fiber laser with variable pulse widths.
Kim, Dong Uk,Song, Hoseong,Song, Woosub,Kwon, Hyuk-Sang,Sung, Miae,Kim, Dug Young Optical Society of America 2012 Optics express Vol.20 No.11
<P>Most of the two-photon fluorescence microscopes are based on femtosecond Ti:Sapphire laser sources near the 800 nm wavelength. Here, we introduce a new confocal two-photon microscope system using a mode-locked Yb(3+)-doped fiber laser. The mode-locked fiber laser produces 13 ps pulses with large positive chirping at a repetition rate of 36 MHz with an average power of 80 mW. By using an external grating pair pulse compressor, the pulse width and the frequency chirping of the laser output are controlled for optimum two-photon excitation. For a given objective lens, the optimum condition was obtained by monitoring the two-photon-induced-photocurrent in a GaAsP photodiode at the sample position. The performance of this pulse width optimized two-photon microscope system was demonstrated by imaging Vybrant DiI-stained dorsal root ganglion cells in 2 and 3 dimensions.</P>
Kim, Dong Uk,Moon, Sucbei,Song, Hoseong,Kwon, Hyuk-Sang,Kim, Dug Young Wiley (John WileySons) 2011 Scanning Vol.33 No.6
<P>High-speed beam scanning and data acquisition in a laser scanning confocal microscope system are normally implemented with a resonant galvanometer scanner and a frame grabber. However, the nonlinear scanning speed of a resonant galvanometer can generate nonuniform photobleaching in a fluorescence sample as well as image distortion near the edges of a galvanometer scanned fluorescence image. Besides, incompatibility of signal format between a frame grabber and a point detector can lead to digitization error during data acquisition. In this article, we introduce a masked illumination scheme which can effectively decrease drawbacks in fluorescence images taken by a laser scanning confocal microscope with a resonant galvanometer and a frame grabber. We have demonstrated that the difference of photobleaching between the center and the edge of a fluorescence image can be reduced from 26 to 5% in our confocal laser scanning microscope with a square illumination mask. Another advantage of our masked illumination scheme is that the zero level or the lowest input level of an analog signal in a frame grabber can be accurately set by the dark area of a mask in our masked illumination scheme. We have experimentally demonstrated the advantages of our masked illumination method in detail.</P>
Cooperative Aerial Manipulation Using Multirotors With Multi-DOF Robotic Arms
Kim, Suseong,Seo, Hoseong,Shin, Jongho,Kim, H. Jin IEEE 2018 IEEE/ASME transactions on mechatronics Vol.23 No.2
<P>This paper investigates pose manipulation of a rod-shaped object using two aerial manipulators, which are multirotors combined with multi-degrees of freedom robotic arms. To achieve the cooperative aerial manipulation, we present motion control and planning methods for aerial manipulators. First, a robust multirotor motion controller is designed based on the thorough analysis on the dynamics of the aerial manipulator. By utilizing extended high-gain observer and disturbance observer techniques, each multirotor can be controlled regardless of the disturbances generated by the robotic arm, the object, and the peer aerial manipulator. Second, a motion planner that assures safety during the cooperative aerial manipulation is proposed. To achieve safe manipulation, we estimate and regulate the internal force between the aerial manipulators. In addition, an unilateral constraint is designed which imposes collision avoidance between the object and aerial manipulators. All the designed features are prioritized and transformed into the reference velocity of the aerial manipulators. The proposed control and guidance laws are validated by a successful autonomous aerial manipulation experiment.</P>
Exploiting Web Images for Video Highlight Detection With Triplet Deep Ranking
Kim, Hoseong,Mei, Tao,Byun, Hyeran,Yao, Ting IEEE 2018 IEEE transactions on multimedia Vol.20 No.9
<P>Highlight detection from videos has been widely studied due to the fast growth of video contents. However, most existing approaches to highlight detection, either handcraft feature based or deep learning based, heavily rely on human-curated training data, which is very expensive to obtain and, thus, hinders the scalability to large datasets and unlabeled video categories. We observe that the largely available Web images can be applied as a weak supervision for highlight detection. For example, the top-ranked images in reference to the query “skiing” returned by a search engine may contain considerable positive samples of “skiing” highlights. Motivated by this observation, we propose a novel triplet deep ranking approach to video highlight detection using Web images as a weak supervision. The approach handles the relative preference of highlight scores between highlighting frames, nonhighlighting frames, and Web images by the triplet ranking constraints. Our approach can iteratively train two interdependent deep models (i.e., a triplet highlight model and a pairwise noise model) to deal with the noisy Web images in a single framework. We train the two models with relative preferences to generalize the capability regardless of the categories of training data. Therefore, our approach is fully category independent and exploits weakly supervised Web images. We evaluate our approach on two challenging datasets and achieve impressive results compared with the state-of-the-art pairwise ranking support vector machines, a robust recurrent autoencoder, and spatial deep convolution neural networks. We also empirically verify through cross-dataset evaluation that our category-independent model is fairly generalizable even if two different datasets do not share exactly the same categories.</P>
GM Volt 실도로 운행 조건에서의 냉난방 시스템 제어 특성 연구
이호성(Hoseong Lee),원종필(Jongphil Won),조중원(Choongwon Cho),이무연(Mooyeon Lee),박용선(Yongsun Park),김성균(Sungkyun Kim),김용찬(Yongchan Kim) 한국자동차공학회 2012 한국자동차공학회 부문종합 학술대회 Vol.2012 No.5
Experiments of electrical air conditioning system for GM Volt(PHEV, Plug-in Hybrid Electric Vehicle) has been performed with the variation of operating conditions. To control temperature in the cabin, GM Volt applying electric drive system has electrical air conditioning system, such as the electric-driven compressor and coolant heating PTC heater. In this study, the electrical air conditioning system layout of GM Volt was founded and temperature control logic in the cabin was partially analyzed from fuel economy test and city driving test modes. When the cabin cooled down, the supply of electric power to the electric-driven compressor seemed to be controled at the interior temperature level of 8℃ during CS(Charge sustaining) mode, which engine operated after the deletion of charging. When the cabin warmed warm under subzero ambient condition, high voltage PTC heater was mainly used at CD(Charge depleting) mode and engine operated intermittently to heat up coolant. Consequently, battery charging depletion got rapid that fuel economy got worse. Even though heating mode was not operated under freezing weather condition, engine operation seemed to be controlled by coolant inlet temperature to PTC heater at the level of 40℃. Further researches for cool-down performance on the road will be studied.