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이정일(Jung Il Lee),박장환(Jang Hwan Park),김석동(Sok Dong Kim),안병옥(Byeong Ok Ahn),이승택(Seung Tack Lee) 한국약용작물학회 1993 한국약용작물학회지 Vol.1 No.1
This study was conducted to select thin-shelled and high-yielding lines in job`s-tears. Two breeding lines of Suwon 3 and Suwon 6 were selected from the local collections. These two lines were tested and investigated on their characteristics under the field condition. The heading date of Suwon 3 and Suwon 6 was later one or two days, but the maturity date was one or two days earlier than that of check variety Kim-jejong, respectively. The number of grains per hill of Suwon 3, Suwon 6 was 50%, 49% greater and the milling rate was 3.8%, 5.6% higher than that of check variety, respectively. Althought 1000 grain weight of Suwon 3 and Suwon 6 was 20g lighter and the rate of ripeness was 6%, 12% lower, the raw grain yield was 22%, 20% higher than that of check variety, respectively. The thickness of seed coat of Suwon 3 and Suwon 6 was thiner and the hardness of seed coat was lower than that of check variety, therefore the milling time was decreased 12%, 7% compare to check variety, respectively. The crude protein contents of Suwon 3 and Suwon 6 was slightly higher and the amino acid composition of Suwon 6 was similar to Kimjejong, but Suwon 3 was lower than that of check variety.
Human erythropoietin induces lung failure and erythrocytosis in transgenic mice /
Kim, Myoung Ok,Kim, Sung Hyun,Shin, Mi Jung,Lee, Dong Beom,Kim, Tae Won,Kim, Kil Soo,Ha, Ji Hong,Lee, Sang Gyu,Park, Yong Bok,Kim, Sun Jung,Ryoo, Zae Young Korean Society for Molecular and Cellular Biology 2007 Molecules and cells Vol.23 No.1
Analysis of Hemolytic Microflora from the Ark Shell (Scapharca broughtonii)
Dong-Gyun Kim(김동균),Bo-Hye Nam(남보혜),Hee Jeong Kong(공희정),Woo-Jin Kim(김우진),Bong-Seok Kim(김봉석),Young-Ju Jee(지영주),Sang-Jun Lee(이상준),Choon Goo Jung(정춘구),Mi-Sun Kong(공미선),Young-Ok Kim(김영옥) 한국생명과학회 2012 생명과학회지 Vol.22 No.5
남해연안지역은 피조개(Scapharca broughtonii) 양식으로 매우 중요한 지역이나 최근 대량폐사로 인하여 생산량이 급격히 감소하였다. 이러한 대량폐사의 원인을 구명하기 위하여 다양한 환경요인들에 대한 연구는 수행되었으나, 미생물학적인 연구는 미비한 실정이다. 본 연구에서는 폐사율이 높은 강진만과 폐사발생이 적은 진해만연안 피조개 양식장의 해저 퇴적물과 피조개 체내에서 분리한 미생물 균총에 대하여 분석하였다. 각각의 샘플에 서 배양 가능한 모든 미생물을 분리하였으며, 분리한 미생물 중 알파 또는 베타 용혈활성을 보이는 미생물을 모두 동정하였다. 피조개 체내에 존재하는 용혈미생물의 균총은 해저 퇴적물 보다 낮은 다양성을 보였으며, 극히 한정된 종의 미생물이 존재함을 알 수 있었다. 강진만에서는 17속(genus) 88종(species)의 미생물을, 그리고 진해만에서는 16속 64종의 미생물을 분리할 수 있었다. 샘플 내에는 Bacillus 속의 미생물이 가장 많이 분리 되었으며, 그 중 Bacillus cereus group의 종이 가장 많고, Paenibacillus, Lynsilbacillus, 그리고 Vibrio 종 등이 그 다음으로 많이 존재하였다. 속(genus) 수준에서는 두 지역의 특별한 차이를 발견할 수 없었으나, 진해만 연안에서는 64종이 발견되었고 강진만에서는 88종이 분리되어, 강진만이 좀 더 종 수준에서의 높은 다양성을 알 수 있었다. 따라서 피조개 대량폐사의 원인은 이러한 균주의 종 수준에서의 차이에서 기인하는 것 이라고 가정할 수 있었으며, 폐사지역에서 발견되는 미생물을 이용한 감염실험을 통하여 피조개의 대량폐사 원인 균을 구명 할 예정이다. The southern coast of Korea is important for the ark shell (Scapharca broughtonii) aquaculture, but the productivity was rapidly reduced during the previous decade by mass mortality. To overcome this economic loss, investigations only focused on environmental factors, and microbiological researches were performed insufficiently. In this study, two sites (Gangjin and Jinhae bay) were selected for their high and low rate of mortality, respectively, and the existence of microflora from underwater sediments in the bodies of S. broughtonii was analyzed. We screened the whole body of each sample and chose unique colonies, which exhibit alpha- and beta-hemolytic activity, for identification. The microflora in S. broughtonii was less variable than sediments, and restricted species were isolated. We identified 17 genera of 88 species and 16 genera of 64 species from the two bays, respectively. A major proportion was comprised of Bacillus species, with the Bacillus cereus group being the most common species among the Bacillus strains, while Paenibacillus, Lynsilbacillus, and Vibrio species were the second most abundant species. At the genus level, there were no significant microbial differences between the two coastal regions. 64 species were isolated from rare site (Jinhae bay), but more species (88) with greater variety were isolated from the frequent site (Gangjin bay). Therefore, it was assumed that the cause of mass mortality lay in the difference in specie-level diversity, and conducting investigations on the diagnosis of pathogenic species by challenging tests using isolated unique species.
Kim, Julan,Kim, Ju-Won,Kim, Dong-Gyun,Nam, Bo-Hye,Kim, Young-Ok,Park, Jung Youn,Kong, Hee Jeong Elsevier 2018 FISH AND SHELLFISH IMMUNOLOGY Vol.79 No.-
<P><B>Abstract</B></P> <P>The tripartite motif-containing (TRIM) proteins are involved in a wide range of cellular processes, and the role of TRIM1 in immunity has been explored. However, fundamental studies on fish TRIM1 are lacking. In this study, we cloned and characterized TRIM1 cDNA from the Korean rose bitterling, <I>Rhodeus uyekii</I> (RuTRIM1). Two RuTRIM1 isoforms (RuTRIM1-X1 and RuTRIM1-X2) were identified. The coding sequence (CDS) of RuTRIM1-X1 comprised 2157 bp encoding a 718-aa protein, and the CDS of RuTRIM1-X2 comprised 1929 bp encoding a 642-aa protein. Both RuTRIM1 isoforms contained a RING finger domain, B-box 1, B-box 2, coiled-coil domain, COS box, FN3 motif, and PRY/SPRY domain. The deduced RuTRIM1-X1 and RuTRIM1-X2 proteins had high amino acid identity (76.27–98.89%) with orthologs from various other species, and a phylogenetic tree was constructed. RuTRIM1-X1 and RuTRIM1-X2 mRNA were expressed in all tissues examined, with the highest expression levels detected in the hepatopancreas. During early development, RuTRIM1-X1 and RuTRIM1-X2 mRNA levels changed differently from the gastrula period to the first feeding stage. An <I>in vivo</I> ubiquitination assay showed that RuTRIM1 exhibited RING-dependent E3 ubiquitin ligase activity, mainly by comparing RuTRIM1-X2 to RuTRIM1-X1. The subcellular localization of the two RuTRIM1 protein isoforms was characterized, revealing that they formed aggregates in cytoplasmic bodies in Raw264.7 cells. Interferon-γ/lipopolysaccharide-induced nuclear factor-κB signaling was negatively regulated by RuTRIM1-X1 and RuTRIM1-X2, and the negative effect was reversed in RING deletion mutants. To our knowledge, this is the first study to characterize fish TRIM1, which may play a role in the inflammatory response.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We isolated two TRIM1 isoforms (RuTRIM1-X1 and RuTRIM1-X2) from <I>Rhodeus uyekii.</I> </LI> <LI> RuTRIM1 cDNAs encode polypeptides with the RBCC motif. </LI> <LI> RuTRIM1 proteins share 76.27–98.89% homology with orthologs from other species. </LI> <LI> The subcellular localization and Ub ligase activity of the two RuTRIM1 protein isoforms were characterized. </LI> <LI> RuTRIM1-X1 and RuTRIM1-X2 negatively regulate the IFN-γ/LPS-induced NF-κB signaling. </LI> </UL> </P>
Cohort profile: National Investigation of Birth Cohort in Korea study 2008 (NICKs-2008)
Kim, Ju Hee,Lee, Jung Eun,Shim, So Min,Ha, Eun Kyo,Yon, Dong Keon,Kim, Ok Hyang,Baek, Ji Hyeon,Koh, Hyun Yong,Chae, Kyu Young,Lee, Seung Won,Han, Man Yong The Korean Pediatric Society 2021 Clinical and Experimental Pediatrics (CEP) Vol.64 No.9
Background: An adequate large-scale pediatric cohort based on nationwide administrative data is lacking in Korea. Purpose: This study established the National Investigation of Birth Cohort in Korea study 2008 (NICKs-2008) based on data from a nationwide population-based health screening program and data on healthcare utilization for children. Methods: The NICKs-2008 study consisted of the Korean National Health Insurance System (NHIS) and the National Health Screening Program for Infants and Children (NHSPIC) databases comprising children born in 2008 (n=469,248) and 2009 (n=448,459) in the Republic of Korea. The NHIS database contains data on age, sex, residential area, income, healthcare utilization (International Classification of Diseases10 codes, procedure codes, and drug classification codes), and healthcare providers. The NHSPIC consists of 7 screening rounds. These screening sessions comprised physical examination, developmental screening (rounds 2-7), a general health questionnaire, and age-specific anticipatory guidance. Results: During the 10-year follow-up, 2,718 children (0.3%) died, including more boys than girls (hazard ratio, 1.145; P<0.001). A total of 848,048 children participated in at least 1 of the 7 rounds of the NHSPIC, while 96,046 participated in all 7 screening programs. A total of 823 infants (0.1%) weighed less than 1,000 g, 3,177 (0.4%) weighed 1,000-1,499 g, 37,166 (4.4%) weighed 1,500-2,499 g, 773,081 (91.4%) weighed 2,500-4,000 g, and 32,016 (5.1%) weighed over 4,000 g. There were 23,404 premature babies (5.5%) in 2008 compared to 23,368 (5.6%) in 2009. The developmental screening test indicated appropriate development in 95%-98% of children, follow-up requirements for 1%-4% of children, and recommendations for further evaluation for 1% of children. Conclusion: The NICKs-2008, which integrates data from the NHIS and NHSPIC databases, can be used to analyze disease onset prior to hospitalization based on information such as lifestyle, eating habits, and risk factors.
The Acceleration Sensor based Personalized Fitness System
Dong-Ok Kim,Min-Suk Gye,Hoe-Kyung Jung 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.1
Information technology and medical advances in technology transformed the paradigm of health. In the past, the focus had been on the treatment, but today the current focus is towards maintaining health. This is research project based on the outdoors, running, walking, biking, in contrast to indoor exercise (weight training) can be utilized in an area where research is lacking. This paper is based on a variety of indoor acceleration sensor movement get figures for the record. This sensor sends data obtained through the Web and market themselves through a suitable exercise program or from users through their Smartphone with purchased recommended programs. Through this research, may be able to have goal-oriented exact figures in the future.