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이정일(Jung Il Lee),박장환(Jang Hwan Park),김석동(Sok Dong Kim),안병옥(Byeong Ok Ahn),이승택(Seung Tack Lee) 한국약용작물학회 1993 한국약용작물학회지 Vol.1 No.1
This study was conducted to select thin-shelled and high-yielding lines in job`s-tears. Two breeding lines of Suwon 3 and Suwon 6 were selected from the local collections. These two lines were tested and investigated on their characteristics under the field condition. The heading date of Suwon 3 and Suwon 6 was later one or two days, but the maturity date was one or two days earlier than that of check variety Kim-jejong, respectively. The number of grains per hill of Suwon 3, Suwon 6 was 50%, 49% greater and the milling rate was 3.8%, 5.6% higher than that of check variety, respectively. Althought 1000 grain weight of Suwon 3 and Suwon 6 was 20g lighter and the rate of ripeness was 6%, 12% lower, the raw grain yield was 22%, 20% higher than that of check variety, respectively. The thickness of seed coat of Suwon 3 and Suwon 6 was thiner and the hardness of seed coat was lower than that of check variety, therefore the milling time was decreased 12%, 7% compare to check variety, respectively. The crude protein contents of Suwon 3 and Suwon 6 was slightly higher and the amino acid composition of Suwon 6 was similar to Kimjejong, but Suwon 3 was lower than that of check variety.
Proteomic analysis of Korean ginseng(Panax ginseng C. A. Meyer) following exposure to salt stress
Kim, Sun-Tae,Bae, Dong-Won,Lee, Kyung-Hee,Hwang, Jung-Eun,Bang, Kyong-Hwan,Kim, Young-Chang,Kim, Ok-Tae,Yoo, Nam-Hee,Kang, Kyu-Young,Hyun, Dong-Yun,Lim, Chae-Oh The Korean Society of Plant Biotechnology 2008 식물생명공학회지 Vol.35 No.3
We evaluated the response to salt stress of two different ginseng lines, STG3134 and STG3159, which are sensitive and tolerant, respectively, to salt treatment. Plants were exposed to a 5 dS/m salt solution, and chlorophyll fluorescence was measured. STG3134 ginseng was more sensitive than STG3159 to salt stress. To characterize the cellular response to salt stress in the two different lines, changes in protein expression were investigated using a proteomic approach. Total protein was extracted from detached salt-treated leaves of STG3134 and STG3159 ginseng, and then separated by two-dimensional polyacrylamide gel electrophoresis(2-DE). Approximately 468 protein spots were detected by 2-DE and Coommassie brilliant blue staining. Twenty-two proteins were found to be reproducibly up- or down-regulated in response to salt stress. Among these proteins, twelve were identified using MALDI-TOF MS and ESI-Q-TOF and classified into several functional groups: photosynthesis-related proteins(oxygen-evolving enhancer proteins 1 and 2, rubisco and rubisco activase), detoxification proteins(polyphenol oxidase) and defense proteins($\beta$-1,3-glucanase, ribonuclease-like storage protein, and isoflavone reductase-like protein). The protein levels of ribonuclease-like storage protein, which was highly induced in STG3159 ginseng as compared to STG3134, correlated tightly with mRNA transcript levels, as assessed by reverse-transcription(RT)-PCR. Our results indicate that salinity induces changes in the expression levels of specific proteins in the leaves of ginseng plants. These changes may, in turn, playa role in plant adaptation to saline conditions.
Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus
Kim, Ju-Won,Oh, Bang Geun,Kim, Julan,Kim, Dong-Gyun,Nam, Bo-Hye,Kim, Young-Ok,Park, Jung Youn,Cheong, JaeHun,Kong, Hee Jeong The Korean Society of Developmental Biology 2018 발생과 생식 Vol.22 No.3
A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 and NANOG were expressed in OFEC-17FEN cells. However, the lack of several pluripotency-related genes expression indicates that OFEC-17FEN cells are not stem cells. OFEC-17FEN cells transfected with plasmid pEGFP-c1 exhibited a strong green fluorescent signal at 48 h after transfection. Accordingly, OFEC-17FEN cells may be useful for both basic research and biotechnological application.
Dong-Jin Kim,Kyung-hwa Han,Yong-seon Zhang,Hee-rae Cho,Seon-ah Hwang,Jung-hun Ok,Kum-Sik Choi,Jung-soon Choi 한국토양비료학회 2018 한국토양비료학회지 Vol.51 No.4
Water is closely linked to agricultural productivity and is an essential resource for agriculture. Climate change and drought are causing water scarcity, and that has an enormous impact on agricultural productivity. Efficient water management methods are needed to prepare for water shortages. The lysimeter is well known as a facility for precisely measuring water and nutrient movement in the soil. Therefore, this study was conducted to investigate the evapotranspiration of different paddy soils using weighable lysimeter and to evaluate the relationship between the evapotranspiration estimated by weighable lysimeter and the reference evapotranspiration estimated by FAO Penman-Monteith equation and Hargreaves equation. This study was performed in lysimeter facility located at the National Institute of Agricultural Sciences, Rural Development Administration, and used lysimeter weight values and meteorological data measured from 1st January to 30th April in 2018. The daily evapotranspiration estimated by the lysimeter was ETLY, the reference evapotranspiration estimated by FAO Penman-Monteith equation was ETPM, and the reference evapotranspiration estimated by Hargreaves equation was ETHS. ETLY showed that loam (L) was higher than that of sandy loam(SL) and silty clay loam (SiCL). The accumulated evapotranspiration from 1st January to 30th April in 2018 was in the order of L (235 mm) > SL (231 mm) > SiCL (192 mm). Solar radiation showed a higher coefficient of determination (R<SUP>2</SUP>) than mean temperature in the correlation between the meteorological data and ETLY. The relationship between ETLY and ETHS showed a relatively low coefficient of determination, whereas the coefficient of determination in the relationship between ETLY and ETPM showed relatively high fitness for SiCL (0.631), L (0.860) and SL (0.884). Precise measurement and management of soil moisture using lysimeter are expected to be possible.
Kim, Su-Young,Lee, Doo-IK,Kim, Keon-Sik,Kim, Dong-Ok,Choi, Young-Kyoo,Choi, Do-Young,Lim, Sabina,Lee, Jae-Dong,Lee, Yun-Hoo 경희대학교 동서의학연구소 2006 東西醫學硏究所 論文集 Vol.2006 No.-
Central poststroke pain can occur as a result of lesion or dysfunction of the brain from stroke, and may influence the autonomic nervous system to regulate the vasomotor activity which could result in the lowered skin temperature. In order to assess CPSP objectively, seventy patients with CPSP were evaluated as their pain with VAS pain score and the skin temperature of pain site by infrared thermography before and after pain treatment. And evaluated correlation between changes of temperature and VAS. The skin temperature of pain site was significantly lower than non-pain before treatment and improved after treatment(p<0.05), in accordance with significant improvement of VAS pain scores after treatment(p<0.05). And there was highly correlation between the changes of temperature and VAS(p<0.05). Therefore, it is suggested that the infrared thermography is very useful device for the evaluation of CPSP and its treatment.
Kim, Dong Yeop,Ko, Kyung Ok,Lim, Jae Woo,Yoon, Jung Min,Song, Young Hwa,Cheon, Eun Jung The Korean Pediatric Society 2018 Clinical and Experimental Pediatrics (CEP) Vol.61 No.12
Purpose: Adenotonsillar hypertrophy (ATH) that causes upper airway obstruction might lead to chronic hypoxemic pulmonary vasoconstriction and right ventricular (RV) dysfunction. We aimed to evaluate whether adenotonsillectomy (T&A) in children suffering from obstructive sleep apnea (OSA) due to severe ATH could improve RV function. Methods: Thirty-seven children (boy:girl=21:16; mean age, $9.52{\pm}2.20years$), who underwent T&A forsleep apnea due to ATH, were included. We analyzedthe mean pulmonary artery pressure (mPAP), the presence and the maximal velocity of tricuspid regurgitation (TR), the tricuspid annular plane systolic excursion (TAPSE), and the right ventricular myocardial performance index (RVMPI) with tissue Doppler echocardiography (TDE) by transthoracic echocardiography pre- and post-T&A. The follow-up period was $1.78{\pm}0.27years$. Results: Only the RVMPI using TDE improved after T&A ($42.18{\pm}2.03$ vs. $40{\pm}1.86$, P=0.001). The absolute value of TAPSE increased ($21.45{\pm}0.90mm$ vs. $22.30{\pm}1.10mm$, P=0.001) but there was no change in the z score of TAPSE pre- and post-T&A ($1.19{\pm}0.34$ vs. $1.24{\pm}0.30$, P=0.194). The mPAP was within normal range in children with ATH, and there was no significant difference between pre- and post-T&A ($19.6{\pm}3.40$ vs. $18.7{\pm}2.68$, P=0.052). There was no difference in the presence and the maximal velocity of TR (P=0.058). Conclusion: RVMPI using TDE could be an early parameter of RV function in children with OSA due to ATH.
Kim, Ok-Tae,Bang, Kyong-Hwan,Shin, Yu-Su,Lee, Min-Jeong,Jung, Su-Jin,Hyun, Dong-Yun,Kim, Young-Chang,Seong, Nak-Sul,Cha, Seon-Woo,Hwang, Baik Springer 2007 Plant cell reports Vol.26 No.11
<P>Transformed root ('hairy root') cultures have been shown to be a good model for the study of many secondary metabolites. However, economically important compounds such as asiaticoside and madecassoside are produced in insignificant amounts in the root of Centella asiatica (L.) Urban. To overcome this problem, C. asiatica was transformed using Agrobacterium rhizogenes strain R1000 that harbors pCAMBIA1302 encoding the hygromycin phosphotransferase (hpt) and green fluorescence protein (mgfp5) genes and the hairy culture was coupled with elicitation technique. Hairy roots were obtained at a frequency of up to 14.1% from a tissue junction between the leaf and petiole. Abundant hairy roots were observed when co-cultivation of the plant with A. rhizogenes was done for 7 days (36.1%). Transformation was confirmed by PCR and Southern blot analyses. Five weeks after inoculation, no asiaticoside was detected in the hairy root samples. However, when 0.1 mM methyl jasmonate (MJ) was applied as an elicitor to the culture medium for 3 weeks, a large quantity of asiaticoside was generated (7.12 mg/g, dry wt). In the case of gene expression, 12 h after MJ treatment the expression of the CabAS (C. asiatica putative beta-amyrin synthase) gene in the hairy roots is significantly different from that of the control and this level of transcripts was maintained for 14 days. Our results showed that production of C. asiatica hairy roots could be optimized and the resulting cultures could be elicited with MJ treatment for enhanced production of asiaticoside.</P>