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- 저자 : Kihwan Lee (검색결과 249 건)
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Lee, Hyungbeen,Lee, Sang Won,Lee, Gyudo,Lee, Wonseok,Nam, Kihwan,Lee, Jeong Hoon,Hwang, Kyo Seon,Yang, Jaemoon,Lee, Hyeyoung,Kim, Sangsig,Lee, Sang Woo,Yoon, Dae Sung The Royal Society of Chemistry 2018 Nanoscale Vol.10 No.2
<P>Here, we demonstrate a powerful method to discriminate DNA mismatches at single-nucleotide resolution from 0 to 5 mismatches (<I>χ</I>0 to <I>χ</I>5) using Kelvin probe force microscopy (KPFM). Using our previously developed method, we quantified the surface potentials (SPs) of individual DNA-capped nanoparticles (DCNPs, ∼100 nm). On each DCNP, DNA hybridization occurs between ∼2200 immobilized probe DNA (<I>p</I>DNA) and target DNA with mismatches (<I>t</I>DNA, ∼80 nM). Thus, each DCNP used in the bioassay (each <I>p</I>DNA-<I>t</I>DNA interaction) corresponds to a single ensemble in which a large number of <I>p</I>DNA-<I>t</I>DNA interactions take place. Moreover, one KPFM image can scan at least dozens of ensembles, which allows statistical analysis (<I>i.e.</I>, an ensemble average) of many bioassay cases (ensembles) under the same conditions. We found that as the <I>χ</I>n increased from <I>χ</I>0 to <I>χ</I>5 in the tDNA, the average SP of dozens of ensembles (DCNPs) was attenuated owing to fewer hybridization events between the <I>p</I>DNA and the <I>t</I>DNA. Remarkably, the SP attenuation <I>vs.</I> the <I>χ</I>n showed an inverse-linear correlation, albeit the equilibrium constant for DNA hybridization exponentially decreased asymptotically as the <I>χ</I>n increased. In addition, we observed a cascade reaction at a 100-fold lower concentration of <I>t</I>DNA (∼0.8 nM); the average SP of DCNPs exhibited no significant decrease but rather split into two separate states (no-hybridization <I>vs.</I> full-hybridization). Compared to complementary <I>t</I>DNA (<I>i.e.</I>, <I>χ</I>0), the ratio of no-hybridization/full-hybridization within a given set of DCNPs became ∼1.6 times higher in the presence of tDNA with single mismatches (<I>i.e.</I>, <I>χ</I>1). The results imply that our method opens new avenues not only in the research on the DNA hybridization mechanism in the presence of DNA mismatches but also in the development of a robust technology for DNA mismatch detection.</P>
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Development of an infant formula certified reference material for the analysis of organic nutrients
Lee, Joonhee,Kim, Byungjoo,Lee, Sun Young,Choi, Jongoh,Kang, Dukjin,Lee, Hwasim,Choi, KiHwan,Lee, Hyeyoung,Sim, Hee-Jung,Baek, Song-Yee,Lee, Honghee,Hyung, Seok-Won,Ahn, Seonghee,Seo, Dongwon,Hwang, J Applied Science Publishers 2019 Food chemistry Vol.298 No.-
<P><B>Abstract</B></P> <P>Infant formula certified reference material (CRM, KRISS CRM 108-02-003) were developed for the analysis of organic nutrients. The CRM is a milk-based infant formula powder, packaged at 14 g per unit. Ten thousand units were prepared and stored at −70 °C. For the certification of each nutrient, ten units were analyzed for simultaneous value-assignment and homogeneity test. Analytical methods used were isotope dilution mass spectrometry (IDMS) based on liquid chromatography mass spectrometer (LC/MS) or gas chromatography mass spectrometer (GC/MS) as higher-order reference methods.13 vitamins, 3 fatty acids, and total cholesterol were certified. The between-unit relative standard deviation of measurement results for each nutrient ranged 0.2% to 2.5%, showing very good homogeneity. The expanded relative uncertainties of the certified values ranged from 1% to 8%, indicating that they have higher-order metrological quality. The values of proximates (proteins, lipids, carbohydrates, water, and ash) were assigned through inter-laboratory comparisons.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An infant formula CRMs for the analysis of organic nutrients was developed. </LI> <LI> Organic nutrients were certified by IDMS approaches as higher-order reference methods. </LI> <LI> Homogeneities and stability of the CRM were evaluated by IDMS approaches. </LI> <LI> Metrological qualities of the certified values were proved by their small uncertainties. </LI> <LI> Five proximates were value-assigned by interlaboratory comparison. </LI> </UL> </P>
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주크박스 뮤지컬 패턴에 대한 고찰: 국내 어트리뷰트 뮤지컬을 중심으로
이기환(Lee Kihwan),이인호(Lee Inho) 한국문화융합학회 2021 문화와 융합 Vol.43 No.1
국내 뮤지컬은 배우의 질적 향상과 해외 스태프와의 협업으로 1990년 이후 가파른 성장을 이루었다. 시장의 발전에 따라 국내 뮤지컬의 제작 편수는 매년 증가하였고, 2005년 이후로는 해외 라이선스 뮤지컬의 제작 편수를 넘어서게 되었다. 그러나 공연 기간과 관객 만족도를 살펴보면 개선이 필요한 실정이다. 이런 상황에서 대안으로 떠오른 것이 과거 히트곡으로 뮤지컬을 제작하는 주크박스 뮤지컬이었다. 본 연구는 국내 어트리뷰트 형식의 주크박스 뮤지컬을 연구하여 뮤지컬에 활용된 원곡이 어떠한 변화를 통해 뮤지컬 넘버로 되었는지를 분석하기 위한 연구이다. 2010년 이후 국내 어트리뷰트 형식의 뮤지컬 중 최근까지 재공연이 지속되고 있는 이영훈의 <광화문연가>, 김광석의 <그날들>, <바람이 불어오는 곳> 세 작품의 대본을 중심으로 공통적인 패턴 변화를 고찰하였다. 그 결과 세 가지의 공통적인 변화 패턴을 알 수 있었는데, 첫째는 가사의 의미변화, 둘째는 필요에 의한 곡의 구조 변형, 마지막으로 극에 맞는 곡 가창 인원의 변화였다. 변형 패턴은 단일보단 중복으로 나타난다는 점이 중요한 부분이었고 모두 극적 효과와 전달하려는 메시지에 중점을 두고 상황에 맞게 이루어졌다. 주크박스 뮤지컬은 원곡이 뮤지컬을 염두에 두고 창작된 곡이 아니기에 상황에 맞는 곡 변형 패턴에 대한 고찰은 주크박스 뮤지컬 제작에 큰 도움이 될 것이다. Since 1990, domestic musicals have developed rapidly due to the actor’s quality improvement and collaboration with overseas staff. As the market grows, the number of domestic musical productions has been increasing annually, and it has exceeded that of foreign licensed musicals since 2005. However, the data on the periods of performances and the audience’s ratings show that there is a need for improvement. In light of the above, Jukebox Musical, which refers to musicals produced using old hit songs, has been proposed as an alternative. This study examines domestic Attribute Jukebox Musicals and analyzes how the original songs change into musical numbers. Among all the domestic Attribute Jukebox Musicals since 2010, “Gwang Hwa Moon Yoen Ga” by Lee Young-hoon, “Gue Nal Duel,” and “Baramee Bureo Onuen Got” by Kim Gwang-seok were studied based on their scripts. Three common patterns of change have been observed: the change of meanings of the lyrics, the adaption of the structure of the songs, and the change in the number of singers who fit the drama. Multiple changes appear more often than just a single touch, which is critically important. The study of these pattern changes will contribute to the production of Jukebox Musicals since the original songs were not created to be performed in a form of musicals.
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Nam, Kihwan,Eom, Kilho,Yang, Jaemoon,Park, Jinsung,Lee, Gyudo,Jang, Kuewhan,Lee, Hyungbeen,Lee, Sang Woo,Yoon, Dae Sung,Lee, Chang Young,Kwon, Taeyun The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.44
<P>We have developed a horizontally aligned carbon nanotube sensor that enables not only the specific detection of biomolecules with ultra-sensitivity, but also the quantitative characterization of binding affinity between biomolecules and/or interaction between a carbon nanotube and a biomolecule, for future applications in early diagnostics. In particular, we have fabricated horizontally aligned carbon nanotubes, which were functionalized with specific aptamers that are able to specifically bind to biomolecules (<I>i.e.</I> thrombin). Our detection system is based on scanning probe microscopy (SPM) imaging for horizontally aligned aptamer-conjugated carbon nanotubes (ACNTs) that specifically react with target biomolecules at an ultra-low concentration. It is shown that the binding affinity between thrombin molecule and ACNT can be quantitatively characterized using SPM imaging. It is also found that the smart carbon nanotube sensor coupled with SPM imaging permits us to achieve the high detection sensitivity even up to ∼1 pM, which is much higher than that of other bioassay methods. Moreover, we have shown that our method enables a quantitative study on small molecule-mediated inhibition of specific biomolecular interactions. In addition, we have shown that our ACNT-based system allows for the quantitative study of the effect of chemical environment (<I>e.g.</I> pH and ion concentration) on the binding affinity. Our study sheds light on carbon nanotube sensor coupled with SPM imaging, which opens a new avenue to early diagnostics and drug screening with high sensitivity.</P> <P>Graphic Abstract</P><P>ACNT, constructed through the chemical conjugation of aptamer to individual nanotube on the silicon substrate, serves as a nanoscale pattern (without lithography) leading to biomolecular recognition with high sensitivity. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm33688j'> </P>
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Park, In Soo,Lee, Jaewoo,Lee, Gyudo,Nam, Kihwan,Lee, Taewoo,Chang, Woo-Jin,Kim, Hansung,Lee, Sei-Young,Seo, Jongbum,Yoon, Dae Sung,Lee, Sang Woo American Chemical Society 2015 ANALYTICAL CHEMISTRY - Vol.87 No.12
<P>Quantitative detection of the biological properties of living cells is essential for a wide range of purposes, from the understanding of cellular characteristics to the development of novel drugs in nanomedicine. Here, we demonstrate that analysis of cell biological properties within a microfluidic dielectrophoresis device enables quantitative detection of cellular biological properties and simultaneously allows large-scale measurement in a noise-robust and probeless manner. Applying this technique, the static and dynamic biological responses of live B16F10 melanoma cells to the small-molecule drugs such as <I>N</I>-ethylmaleimide (NEM) and [(dihydronindenyl)oxy]alkanoic acid (DIOA) were quantitatively and statistically examined by investigating changes in movement of the cells. Measurement was achieved using subtle variations in dielectrophoresis (DEP) properties of the cells, which were attributed to activation or deactivation of K<SUP>+</SUP>/Cl<SUP>–</SUP> cotransporter channels on the cell membrane by the small-molecule drugs, in a microfluidic device. On the basis of quantitative analysis data, we also provide the first report of the shift of the complex permittivity of a cell induced by the small-molecule drugs. In addition, we demonstrate interesting quantifiable parameters including the drug effectiveness coefficient, antagonistic interaction coefficient, kinetic rate, and full width at half-maximum, which corresponded to changes in biological properties of B16F10 cells over time when NEM and DIOA were introduced alone or in combination. Those demonstrated parameters represent very useful tools for evaluating the effect of small-molecule drugs on the biological properties of cells.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2015/ancham.2015.87.issue-12/ac5041549/production/images/medium/ac-2014-041549_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac5041549'>ACS Electronic Supporting Info</A></P>
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Lee, Sangyeob,Lee, Jun Su,Jang, Jiseong,Hong, Ki-Ha,Lee, Doh-Kwon,Song, Soomin,Kim, Kihwan,Eo, Young-Joo,Yun, Jae Ho,Gwak, Jihye,Chung, Choong-Heui Elsevier 2018 Nano energy Vol.53 No.-
<P><B>Abstract</B></P> <P>We demonstrate the ability to fabricate high-quality nanoscale electrical contact between silver nanowires (AgNWs) and underlying semiconducting layers in chalcogenide thin film solar cells. AgNW electrodes have attracted many interests due to their ability for low temperature solution processing. However, they have a drawback that the interfacial defects can be generated between AgNWs and underlying rugged semiconductor layers making it difficult to form high-quality junction. To enhance the junction properties, conducting matrix layers have been adapted. Yet, the issues regarding the AgNW/semiconductor junction have not been fully resolved. We developed a facile method to form robust nanoscale contact between AgNWs and semiconducting thin films to achieve high performance chalcogenide thin film solar cells. The method is to deposit an ultra-thin semiconductor layer on devices using aqueous chemical bath deposition. The chemical bath deposition has capability to effectively fill even nanoscale gap and to form chemically stable bonds as well as an intimate junction. As a proof of concept, a CdS layer (~ 10 nm) was deposited using the chemical bath deposition on Cu(In,Ga)Se<SUB>2</SUB> (CIGS) solar cells with a structure of AgNW/CdS/CIGS/Mo/Glass. We also identified that the key factor governing the current-voltage characteristic is the electrical contact between the AgNW electrode and the CdS buffer layer in CIGS thin film solar cells. The power conversion efficiency of the CIGS cell was dramatically improved from 4.9% to 14.2% owing to high-quality AgNW-CdS electrical contact produced by chemical bath deposition of the additional CdS layer as thin as 10 nm.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A facile method to form robust nanoscale contact between AgNWs and semiconductors for high performance solar cell. </LI> <LI> Formation of high quality junction between AgNWs and a CdS buffer layer in CIGS solar cells by chemical bath deposition. </LI> <LI> Due to the high quality contact, the efficiency of CIGS solar cells dramatically improves from 4.9% to 14.2%. </LI> <LI> The cells with high quality junction show high performance at any illumination conditions. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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