Oral Delivery of Near-Infrared Quantum Dot Loaded Micelles for Noninvasive Biomedical Imaging

Khatun, Zehedina,Nurunnabi, Md,Cho, Kwang Jae,Lee, Yong-kyu American Chemical Society 2012 ACS APPLIED MATERIALS & INTERFACES Vol.4 No.8

<P>The purpose of this study is to design, develop, and characterize an optical imaging agent for oral administration. The hydrophobic, nanosized (7 nm), near-infrared (NIR) quantum dots (QDs) have been loaded into deoxycholic acid (DOCA) conjugated low molecular weight heparin (LMWH) micelles. The QD-loaded LMWH-DOCA (Q-LHD) nanoparticles have been characterized by electrophoretic light scattering (ELS) and a transmission electron microscope (TEM) which shows the average particle size was 130–220 nm in diameter. The Q-LHD nanoparticles also show the excellent stability in different pH conditions, and the release profile demonstrates the slow release of QDs after 5 days of oral administration. Concfocal laser microscopic scanning images show that the Q-LHD nanoparticles penetrate the cell membrane and are located inside the cell membrane. The real time pharmacokinetics studies show the absorption, distribution, metabolism, and elimination profile of Q-LHD nanoparticles, observed by the Kodak molecular imaging system (KMIS). This study has demonstrated that the orally administered Q-LHD nanoparticles are absorbed in the small intestine through the bile acid transporter and eliminated through the kidneys.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2012/aamick.2012.4.issue-8/am301048m/production/images/medium/am-2012-01048m_0010.gif'></P>

Oral absorption mechanism and anti-angiogenesis effect of taurocholic acid-linked heparin-docetaxel conjugates

Khatun, Z.,Nurunnabi, M.,Cho, K.J.,Byun, Y.,Bae, Y.H.,Lee, Y.k. Elsevier Science Publishers 2014 Journal of controlled release Vol.177 No.-

Oral delivery is the preferred route to deliver therapeutics via nanoparticles due to ease of administration and patient acceptance. Here, we report on the findings of the absorption pathway of taurocholic acid (TCA)-linked heparin and docetaxel (DTX) conjugate, which we refer to as HDTA. We studied the oral absorption of HDTA using a Caco-2 cell transport system and an animal model. We have also used other absorption enhancers, such as ethylene glycol tetraacetic acid (EGTA), or inhibitors, such as sodium azide, to compare the relative permeability of HDTA conjugates. In vivo comparative studies were conducted using free TCA as a pre-administration and exhibited the maximum absorption site of the organ after oral administration of HDTA conjugates. HDTA was found to be absorbed mainly in the ileum and Caco-2 cell monolayer through passive diffusion and bile acid transporters. High fluorescence intensity of HDTA in mice came from the ileum, and it was eliminated from the body through colon. This novel formulation could be further investigated by clinical trials to find the prospect of oral anti-cancer drug delivery through anti-angiogenic treatment strategies.

Bioreducible Poly(ethylene glycol)–Triphenylphosphonium Conjugate as a Bioactivable Mitochondria-Targeting Nanocarrier

Khatun, Zehedina,Choi, Yeon Su,Kim, Yu Gyeong,Yoon, Kwonhyeok,Nurunnabi, Md,Li, Li,Lee, Eunji,Kang, Han Chang,Huh, Kang Moo AMERICAN CHEMICAL SOCIETY 2017 Biomacromolecules Vol. No.

<P>Bioactivable nanocarrier systems have favorable characteristics such as high cellular uptake, target specificity, and an efficient intracellular release mechanism. In this study, we developed a bioreducible methoxy polyethylene glycol (mPEG) triphenylphosphonium (TPP) conjugate (i.e., mPEG (ss-TPP)(2) conjugate) as a vehicle for mitochondrial drug delivery. A bioreducible linkage with two disulfide bond containing end groups was used at one end of the hydrophilic mPEG for conjugation with lipophilic TPP molecules. The amphiphilic mPEG (ss-TPP)(2) self-assembled in aqueous media, which thereby formed core shell structured nano particles (NPs) with good colloidal stability, and efficiently encapsulated the lipophilic anticancer drug doxorubicin (DOX). The DOX-loaded mPEG (ss-TPP)(2) NPs were characterized in terms of their physicochemical and morphological properties, drug-loading and release behaviors, in vitro anticancer effects, and mitochondria-targeting capacity. Our results suggest that bioreducible DOX-loaded mPEG (ss-TPP)(2) NPs can induce fast drug release with enhanced mitochondrial uptake and have a better therapeutic effect than nonbioreducible NPs.</P>

Molecular Characterization of Selected Local and Exotic Cattle Using RAPD Marker

Khatun, M. Mahfuza,Hossain, Khondoker Moazzem,Rahman, S.M. Mahbubur Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.6

In order to develop specific genetic markers and determine the genetic diversity of Bangladeshi native cattle (Pabna, Red Chittagong) and exotic breeds (Sahiwal), randomly amplified polymorphic DNA (RAPD) analysis was performed using 12 primers. Genomic DNA was extracted from 20 cattle (local and exotic) blood samples and extracted DNA was observed by gel electrophoresis. Among the random primers three were matched and found to be polymorphic. Genetic relations between cattle's were determined by RAPD polymorphisms from a total of 66.67%. Statistical analysis of the data, estimating the genetic distances between cattle and sketching the cluster trees were estimated by using MEGA 5.05 software. Comparatively highest genetic distance (0.834) was found between RCC-82 and SL-623. The lowest genetic distance (0.031) was observed between M-1222 and M-5730. The genetic diversity of Red Chittagong and Sahiwal cattle was relatively higher for a prescribed breed. Adequate diversity in performance and adaptability can be exploited from the study results for actual improvement accruing to conservation and development of indigenous cattle resources.

Effect of Aminopeptidase N on functions and fertility of mouse spermatozoa in vitro

Khatun, Amena,Kang, Kyu-Ho,Ryu, Do-Yeal,Rahman, Md Saidur,Kwon, Woo-Sung,Pang, Myung-Geol Elsevier 2018 Theriogenology Vol.118 No.-

<P><B>Abstract</B></P> <P>Aminopeptidase N (APN) is defined as a multifunctional enzyme, which regulate cellular physiology of a wide variety of cells in human. Earlier studies reported that mammalian semen shares this common enzyme as a major protein of seminal plasma that has correlation with male fertility, while the regulatory mechanisms of APN in spermatozoa are still far from being well understood. Present study was designed to investigate the role of APN in biological and chemical functions of spermatozoa using an in vitro antagonistic approach. Results showed that lower APN activity in sperm culture medium significantly increased sperm motility and the percentage of high speed spermatozoa and decreased the percentage of slow speed spermatozoa after a dose dependent inhibitor treatment (10, 100, and 1000 μM leuhistin) on epididymal mouse spermatozoa in a capacitating media for 90 min. Both 100 μM and 1000 μM decreased APN activity, while only 1000 μM decreased cell viability and increased PKA activity significantly compared to control. Nonetheless capacitation status, acrosome reaction status, and lactate dehydrogenase activity were not affected. Intriguingly, the treatment affected embryonic development through decreasing tyrosine phosphorylation of proteins and increasing reactive oxygen species levels. Further <I>in silico</I> analysis revealed associated regulatory proteins, which have critical functional role for male fertility.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Inhibition of APN activity in sperm culture medium significantly increased motility. </LI> <LI> Treatments for APN inhibition have decreased APN activity and increased PKA levels. </LI> <LI> Intriguingly, inhibition of APN activity in sperm culture medium had a detrimental effect on early embryogenesis. </LI> <LI> In silico analysis of this enzyme predicted associated regulatory proteins, which have critical functional role in male fertility. </LI> </UL> </P>

A review on the recent advancements in nanomaterials for nonenzymatic lactate sensing

Khatun A. Jannath,Md Mobarok Karim,Heru Agung Saputra,Kyeong-Deok Seo,김광복,심윤보 대한화학회 2023 Bulletin of the Korean Chemical Society Vol.44 No.5

Lactate is one of the major biomarkers to assess the physical fitness of human in clinical or sports medicine. The proper and well-timed lactate determination can avoid some exigent conditions including hemorrhage, respiratory distress, sepsis, and hypoxia. Therefore, rapid, facile, selective, and reliable detection of lactate have gained appreciation lately. Among the various lactate detection methods, electrochemical method is the most rapid, convenient, and sensitive technique. Specifically, nanomaterial-based nonenzymatic electrochemical lactate sensors are much desirable to solve the demerits and stability issues of enzymatic lactate sensors. Numerous materials including metal and metal oxide nanoparticles, metal–organic frameworks, molecularly imprinted polymers, and carbons were used as electrocatalysts to achieve highly sensitive lactate sensors. This present review mainly focuses on the recent developments of these materials for enzyme-free lactate oxidation and the future prospective of electrode modification catalysts for lactate sensors.

Molecular Characterization and Expression Profiling of Tomato GRF Transcription Factor Family Genes in Response to Abiotic Stresses and Phytohormones

Khatun, Khadiza,Robin, Arif Hasan Khan,Park, Jong-In,Nath, Ujjal Kumar,Kim, Chang Kil,Lim, Ki-Byung,Nou, Ill Sup,Chung, Mi-Young MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.5

<P>Growth regulating factors (GRFs) are plant-specific transcription factors that are involved in diverse biological and physiological processes, such as growth, development and stress and hormone responses. However, the roles of GRFs in vegetative and reproductive growth, development and stress responses in tomato (<I>Solanum lycopersicum</I>) have not been extensively explored. In this study, we characterized the 13 <I>SlGRF</I> genes. In silico analysis of protein motif organization, intron–exon distribution, and phylogenetic classification confirmed the presence of GRF proteins in tomato. The tissue-specific expression analysis revealed that most of the <I>SlGRF</I> genes were preferentially expressed in young and growing tissues such as flower buds and meristems, suggesting that <I>SlGRFs</I> are important during growth and development of these tissues. Some of the <I>SlGRF</I> genes were preferentially expressed in fruits at distinct developmental stages suggesting their involvement in fruit development and the ripening process. The strong and differential expression of different <I>SlGRFs</I> under NaCl, drought, heat, cold, abscisic acid (ABA), and jasmonic acid (JA) treatment, predict possible functions for these genes in stress responses in addition to their growth regulatory functions. Further, differential expression of <I>SlGRF</I> genes upon gibberellic acid (GA3) treatment indicates their probable function in flower development and stress responses through a gibberellic acid (GA)-mediated pathway. The results of this study provide a basis for further functional analysis and characterization of this important gene family in tomato.</P>

Genetic diversity and geographic distribution of the <i>Bemisia tabaci</i> species complex in Bangladesh

Khatun, M.F.,Jahan, S.M.H.,Lee, Sukchan,Lee, Kyeong-Yeoll Elsevier 2018 Acta Tropica Vol.187 No.-

<P><B>Abstract</B></P> <P> <I>Bemisia tabaci</I> (Gennadius) is a species complex consisting of at least 40 cryptic species. Although the genetic diversity of <I>B. tabaci</I> has been studied in various regions, little is known about distribution in Bangladesh, which is covered by the Bengal delta, the largest delta on Earth. We conducted an extensive survey throughout the country and determined the nucleotide sequence of mitochondrial cytochrome <I>c</I> oxidase subunit 1 (COI) from 110 individuals. We then examined phylogenetic relationships. The results identified four cryptic species that expressed distinct interspecific variation but low intraspecific variation. Asia I was the most abundant, both Asia II 1 and Asia II 5 were moderately abundant, and Asia II 10 was found only in the central region. COI sequences of each cryptic species were distinctive and differentiated into many haplotypes. Our study provides important information to better understand the genetic diversity and geographic distribution of cryptic species in Bangladesh and nearby countries.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Genetic diversity of <I>Bemisia tabaci</I> was determined in Bangladesh. </LI> <LI> We identified four indigenous cryptic species but not MEAM1 and MED invasive cryptic species. </LI> <LI> Asia I was abundant, both Asia II 1 and Asia II 5 were moderate, and Asia II 10 was found only in the central region. </LI> <LI> Our study provides important information on the genetic diversity and geographic distribution of <I>B. tabaci</I> in Bangladesh. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Expression Profiling of the CSDP Transcription Factor Gene Family Points to Roles in Organ Development and Abiotic Stress Response in Tomato (Solanum lycopersicum L.)

Khatun, Khadiza,Nath, Ujjal Kumar,Park, Jong-In,Kim, Chang Kil,Nou, Ill Sup,Chung, Mi-Young Springer-Verlag 2018 Plant molecular biology reporter Vol.36 No.2

Visible-Light Photoredox-Catalyzed Hydroalkoxymethylation of Activated Alkenes Using α-Silyl Ethers as Alkoxymethyl Radical Equivalents

Khatun, Nilufa,Kim, Myeong Jun,Woo, Sang Kook American Chemical Society 2018 ORGANIC LETTERS Vol.20 No.19

<P>A new neutral silicon-based traceless activation group (TAG) for visible-light photoredox-catalyzed hydroalkoxymethylation of alkenes is presented. This reaction involves in-situ-generated alkoxymethyl radical via single electron oxidation (SET) of α-TMS-substituted ethers, followed by subsequent conjugate addition to activated alkenes. Various functional groups were tolerated both under mild metal and metal-free conditions to provide good to excellent yields. Furthermore, the addition products were transformed to valuable synthetic building blocks, such as carboxylic acids, γ-butyrolactones, and complex aryl alkyl ethers.</P> [FIG OMISSION]</BR>