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        Synthesis and characterization of PANI/MOF-199/Ag nanocomposite and its potential application as non-enzymatic electrochemical sensing of dopamine

        Nisar Asia,Khan Muzamil Ahmad,Hussain Zakir 한국세라믹학회 2022 한국세라믹학회지 Vol.59 No.3

        In this research study, a highly selective and sensitive electrochemical sensor was designed by coating the glassy carbon electrode (GCE) with the novel PANI/MOF-199/Ag nanocomposite for the non-enzymatic electrochemical detection of dopamine (DA). PANI/MOF-199/Ag nanocomposite was prepared by the in situ chemical oxidative polymerization of aniline monomer in the presence of MOF-199 followed by the deposition of Ag nanoparticles using an in situ chemical reduction route. Characterization techniques such as Scanning electron microscopy (SEM), X-ray powder diffraction (XRD), Fourier transform infra-red spectroscopy (FTIR), and Raman analysis were used to find out the successful synthesis of the prepared samples. Meanwhile, the electrochemical performance was investigated by chronoamperometry (CA) and cyclic voltammetry (CV) not only at different molar concentrations of DA but also with various compositions of Ag nanoparticles loaded. The PANI/MOF-199/Ag-3% modified electrode exhibited good electro-catalytic activity with high sensitivity of 1.421 μA μM−1 cm− 2 and a low detection limit (LOD) of 0.22 μM (S/N = 3) towards DA. Moreover, in the presence of common interfering biological compounds such as glucose (Glu), uric acid (UA), and ascorbic acid (AA), the fabricated electrochemical sensor was found selective to DA detection.

      • KCI등재후보

        Effect of Saffron (Crocus sativus) on Neurobehavioral and Neurochemical Changes in Cerebral Ischemia in Rats

        Fakhrul Islam,Sofiyan Saleem,Muzamil Ahmad,Abdullah Shafique Ahmad,Seema Yousuf,Mubeen Ahmad Ansari,M. Badaruzzaman Khan,Tauheed Ishrat 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.2

        The modifying effects of Crocus sativus(CS) stigma extract on neurobehavioral activities, malondialdehyde(MDA), reduced glutathione (GSH), glutathione peroxidase, glutathione reductase, glutathione S-transferase, superoxide dis-mutase (SOD), catalase (CAT), and Na. ,K. -ATPase activities, and glutamate (Glu) and aspartate (Asp) content were exam-ined in the middle cerebral artery (MCA) occlusion (MCAO) model of acute cerebral ischemia in rats. The right MCA ofmale Wistar rats was occluded for 2 hours using intraluminal 4-0 monofilament, and reperfusion was allowed for 22 hours.MCAO caused significant depletion in the contents of GSH and its dependent enzymes while significant elevation of MDA,Glu, and Asp. The activities of Na. ,K. -ATPase, SOD, and CAT were decreased significantly by MCAO. The neurobehav-ioral activities (grip strength, spontaneous motor activity, and motor coordination) were also decreased significantly in theMCAO group. All the alterations induced by ischemia were significantly attenuated by pretreatment of CS (100 mg/kg ofbody weight, p.o.) 7 days before the induction of MCAO and correlated well with histopathology by decreasing the neuronalcell death following MCAO and reperfusion. The present results may suggest the effectiveness of CS in focal ischemia mostprobably by virtue of its antioxidant property.

      • KCI등재후보

        Behavioral and Histologic Neuroprotection of Aqueous Garlic Extract After Reversible Focal Cerebral Ischemia

        Fakhrul Islam,Sofiyan Saleem,Muzamil Ahmad,Abdullah Shafique Ahmad,Seema?ousuf,Mubeen Ahmad Ansari,M. Badruzzaman Khan,Tauheed Ishrat 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.4

        The objective of the present study was to investigate the effects of aqueous garlic extract (AGE) on neurobe-havioral activities, malondialdehyde (MDA) and reduced glutathione (GSH) levels, glutathione peroxidase (GPx), glutathionereductase (GR), glutathione S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), and sodium-potassium ATPase (Na. ,K. -ATPase) activities, and glutamate and aspartate content in a middle cerebral artery (MCA) occlusion(MCAO) model of acute cerebral ischemia in rats. The right MCA of male Wistar rats was occluded for 2 hours using intra-luminal 4-0 monofilament, and reperfusion was allowed for 22 hours. MCAO caused significant depletion in GSH and its dependent enzymes (GPx, GR, and GST) and significant elevation of MDA, glutamate, and aspartate. The activities of Na. ,K. -ATPase, SOD, and CAT were decreased significantly by MCAO. The neurobehavioral activities (grip strength, spontaneousmotor activity, and motor coordination) were also decreased significantly in the MCAO group. All of the alterations inducedby ischemia were significantly attenuated by pretreatment with AGE (500 mg/mL/kg of body weight, i.p.) 30 minutes beforethe induction of MCAO and correlated well with histopathology by decreasing the neuronal cell death following MCAO andreperfusion. These findings suggest that AGE effectively modulates neurobehavioral and neurochemical changes in focal is-chemia, most probably by virtue of its antioxidant properties.

      • KCI등재

        Preparation of porous ZIF-67 network interconnected by MWCNTs and decorated with Ag nanoparticles for improved non-enzymatic electrochemical glucose sensing

        Elizbit,Liaqat Usman,Hussain Zakir,Baig Mutawara Mahmood,Khan Muzamil Ahmad,Arif Dooa 한국세라믹학회 2021 한국세라믹학회지 Vol.58 No.5

        Due to porous structure, larger specifi c area, and potential to functionalize further, metal–organic frameworks (MOFs) are gaining attention in various fi elds, including electrochemical sensing of various analytes. MOFs are known to have excellent electrochemical activity because they mainly constitute a rich metallic center, possess huge specifi c area, and have multiple valence states. However, electrocatalytic activities of MOFs are greatly reduced due to their poor electronic conductivity for which high conductivity species are incorporated into the MOFs. In the present work, Co-based MOF (ZIF-67) was used as a porous matrix to encapsulate silver nanoparticles (Ag NPs) and on the surface of ZIF-67 multi-walled carbon nanotubes (MWCNTs) were decorated to obtain Ag@ZIF-67/MWCNT nanocomposite for the non-enzymatic electrochemical detection of glucose. The surface morphology of the synthesized nanocomposite was investigated by scanning electron microscopy, while crystallographic characterization was performed by X-ray powder diff raction. Cyclic voltammetry and chronoamper- ometry techniques were used to for the electrochemical monitoring of glucose. The electrochemical results demonstrated that Ag@ZIF-67/MWCNT showed an improved catalytic activity towards glucose in the concentration range of 33 μM to 400 μM with a low detection limit of 0.49 μM (S/N = 3), and reaction sensitivity of 13.014 μA μM −1   cm −2 as well as good selectivity in presence of ascorbic acid and uric acid.

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