대학생 잠복결핵 감염의 진단

육근돌 ( Keun-dol Yook ),양병선 ( Byoung-seon Yang ) 대한임상검사과학회 2016 대한임상검사과학회지(KJCLS) Vol.48 No.3

결핵이란 결핵균에 의한 만성 감염질환으로 결핵균이 포함된 비말액을 통해 공기감염을 일으킨다. 대부분의 결핵감염자는 전염력이 없는 잠복감염상태만 유지하나 10%의 감염자 중 절반은 감염 후1∼2년 안에 발병하게 된다. 결핵감염자와 접촉이 있는 학생 74명을 대상으로 결핵 감염자 접촉관련 사전 조사 및 흉부 X-선 검사, TST 검사 및 IGRA 검사를 실시하였다. 1차 TST 검사에서 양성자는 9명, 음성은 65명으로 나타났으며, 음성 판독자는 2차 TST검사를, 양성 판독자는 IGRA 검사를 실시하였다. 1차 TST 양성자 9명중 IGRA 검사에서 3명이 양성자로 나와 잠복 결핵감염 치료를 실시하였다. 1차 TST결과 음성자에 대한 재확인 TST 검사는 1차와 차이가 없었다. 결론적으로 잠복결핵감염율은 74명중 3명으로 4.05%로 나타났고, 잠복결핵감염자 색출을 위해서 다른 그룹에 대한 조사도 필요하다. Tuberculosis (TB) is caused by a chronic infectious agent known as Mycobacterium tuberculosis. It is transmitted in airborne particles, called droplet nuclei which was generated by cough, sneeze, shout, or sing of persons who have TB disease. Most infections of TB do not have symptoms, well known as latent tuberculosis infection (LTBI). However, about 10% of LTBI progress to active disease a one or two years after infection. To investigate the LTBI rate of college students who were in contacted with TB patients, we performed chest X-ray, tuberculin skin test (TST) and Interferon-gamma release assay (IGRA) to 74 college students. At a results, 65 students were showed negative and 9 students positive results at chest X-ray and 1st TST test. When confirmed the 65 students who were showed negative by 2st TST, the results showed correctly. But, 9 students who were showed positive results on chest X-ray and 1st TST by IGRA, the only 3 students (4.05%) showed positive results. In conclusion, the LTBI rate in this study showed 4.05% (3/74) and we suggest to investigate other students LTBI rate for decreasing tuberculosis.

지방산분석을 이용한 Pseudomonas aeruginosa의 동정

육근돌 ( Keun Dol Yook ) 대한임상검사과학회 2005 대한임상검사과학회지(KJCLS) Vol.37 No.3

Cellular fatty acid composition of 17 strains of the Pseudomonas aeruginosa was determined by gas-liquid chromatography isolated from environmental and clinical sample in a C university hospital. Straight-chain saturated acid of C16:0 and straight-chain unsaturated acid of C18:1 with a double bond were commonly found in all the strains tested. The presence of 12:0 3OH (3-10%), 16:0 (18-28%), and 18:1w7c (17-37%) showed the characteristics of the species in the Pseudomonas. Bacterial fatty acid composition was considered to be useful for the study of interrelation and for rapid identification of the bacteria.

대전지역에서 그람음성간균의 항생제 내성률 조사

육근돌 ( Keun Dol Yook ) 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.3

During 2006, the antibiotic resistance rate were investigated in gram negative bacteria. Resistance to piperacillin were detected at 60% in Escherichia coli, and 37% in Klebsiella pneumoniae. Ceftriaxone were detected 58% in Enterobacter cloacae, 52% in Acinetobacter baumannii, 43% in Enterobacter aerogenes and 76% were detected in Serratia marcescens. Between 1998 and 2007 antibiotic resistance rate were decreased in seven types antibiotic drugs. but, ceftazidime were increased from 12 to 20% during this times. In addition, E. coli, E. cloacae, A. baumannii and E. aerogenes were more isolated from May to June and K. pneumoniae and S. marcescens were more isolated from July to September. We should monitor and control antibiotic use and regularly survey antibiotic resistance patterns among pathogens in the hospital.

폐렴 막대균에 대한 현삼(Scrophularia ningpoensis hemsl) 추출액의 항균력과 세포독성 시험

육근돌(Keun-Dol Yook) 한국컴퓨터정보학회 2016 韓國컴퓨터情報學會論文誌 Vol.21 No.5

Scrophularia ningpoensis hemsl has been traditionally used in China and Vietnam for treatment of bacteria, atopy, pimple, tonsillitis, angina and encephalitis for a long time. The main objectives of this study were to evaluate the antibacterial activity of the Scrophularia ningpoensis hemsl extract on biofilm formation of Klebsiella pneumoniae. Antibacterial activity was conducted using disc diffusion assay and minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were determined using the broth micro dilution method in accordance to Clinical and Laboratory Standards Institute guidelines(CLSI). Furthermore, cytotoxicity on L929 were assessed using animal cell culture for the proliferation test(MTT cell assay) and the biofilm forming capacity of the K. pneumoniae were determined using the colony forming unit (CFU) assay. The extract exhibited considerable antibacterial activity. K. pneumoniae was susceptible to the extract with the MIC and MBC of 0.1875 and 1.5 ㎎/㎖ respectively. Cytoxicity test in L929 showed no sign of toxicity at the concentration of 0.75㎎/㎖ and at the same concentration the extract caused inhibition of bacterial biofilm formation. The extract of Scrophularia ningpoensis hemsl possesses an in vitro antibacterial antibiofilm activities against K. pneumoniae, with no sign of cytoxicity on L929.

Extended-Spectrum β-Lactamase 생성 Klebsiella pneumoniae 균주의 유전형 검출

육근돌(Yook, Keun-Dol),양병선(Yang, Byoung-Seon),박진숙(Park, Jin-Sook) 한국산학기술학회 2013 한국산학기술학회논문지 Vol.14 No.3

임상검체에서 분리되는 그람음성 막대균의 제 3세대 cephalosporin에 대한 내성율의 증가는 임상적으로 심각한 문제가 되고 있다. 3세대 cephalosporin 및 monobactam계 항균제에 대한 내성은 주로 Extended-Spectrum β-Lactamase (ESBL)의 생성에 기인한다. 따라서 ESBL 유전자의 정확한 검출은 병원내의 감염경로 파악을 위한 감시 및 역학조사를 위해 필수적이다. 본 연구는 2012년 2월부터 8월까지 대전, 충남, 충북지역의 대학병원으로부터 ESBL 생성 Klebsiella penumoniae 46균주를 분리하여 Clinical and Laboratory Standards Institute (CLSI)에 따라 ceftazidime (CAZ)과 CAZ/clavulanate (CLA)를 이용한 combination disk test (CDT) 방법에 의해 표현형을 조사하고, 유전형 특이 프라이머를 이용한 multiplex PCR을 수행하여 유전형을 검출하였다. CDT 결과 42균주가 ESBL생성균주로 확인되었다. PCR 결과, 46균주 모두 TEM형이었으며, 37균주는 SHV형, 14균주는 CTX-M형으로 나타났으며 10균주가 TEM, SHV, CTX-M 유전 자를 모두 가지고 있었다. Multiplex PCR에 의한 유전형 검출 방법은 임상에서 분리한 ESBL생성 K. penumoniae균주의 감별과 검출에 유용한 방법으로 사료된다. Among Gram-negative pathogens in Korea, the incidence of resistance to third generation cephalosporins is becoming an ever-increasing problem. The production of extended-spectrum β-lactamase (ESBL) is the main mechanism of bacterial resistance to a third-generation cephalosporins and monobactams. Accurate identification of the ESBL genes are necessary for surveillance and epidemiological studies of the mode of transmission in the hospital. This study was conducted to detect the genes encoding ESBL of 46 K. penumoniae isolated from Daejeon, Chungnam and Chungbuk regional university hospitals from February to August in 2012. The phenotypes of the isolated specimens were examined according to the combination disc test (CDT) by the Clinical and Laboratory Standards Institute (CLSI). Forty two ESBL producing K. penumoniae isolates could be detected using ceftazidime (CAZ) discs with and without clavulanate (CLA). By CDT, 42 K. pneumoniae strains were confirmed to be ESBL strains. Genotyping was performed by multiplex PCR with type-specific primers. By PCR analysis, TEM gene in 46 strains, SHV gene in 37 strains and CTX-M genes in 14 strains were identified. Ten isolates did carry genes encoding ESBLs of all types TEM, SHV and CTX-M. The multiplex polymerase chain reaction (PCR) analysis was better to detect and differentiate ESBL producing K. penumoniae strains in clinical isolates.

충청 지역에서 분리된 대장균이 생성하는 Extended Spectrum β-Lactamase 유형의 검출 빈도

육근돌(Yook, Keun-Dol),박진숙(Park, Jin-Sook) 한국산학기술학회 2014 한국산학기술학회논문지 Vol.15 No.4

장내세균 가운데서 가장 빈번하게 나오는 대장균에서 Extended-Spectrum β-Lactamase(ESBL)를 생성하는 효소의 검출빈도와 이들 균주의 ESBL효소의 유형을 구분하는 것이 이 연구의 목적이다.균주는 충청지역 (대전,충남,충북)병원에 서 2013년 2월부터 7월까지 6개월간 282대장균 균주 중 ESBL을 생성하는 74균주(26.2%)를 수집하였다.충청지역에 ESBL 균주를 포함한 대장균의 항균제 내성률은 Aztreonam 30.8%,Cefotaxim 30.9%,그리고 Ceftazidime32.2%로 나타났으며 ESBL을 생성하는 균주는 β-lactam 항균제인 Aztreonam에 58.1%,Cefotaxim 100%,그리고 Ceftazidime63.5%의 내성률을 보였다.동정된 ESBL균의 CTX-M-2은 48균주,CTX-M-8은 20균주,PER-128균주,그리고 VEB-126균주에서 나왔 다.GES-1형은 74균주 중 2균주가 충남에서만 유일하게 나타났다.ESBL검출빈도와 유형의 정확한 파악은 병원감염관리와 항균제 처방에 도움이 될 것이다. The study aims primarily to evaluate the resistance of antibiotics and the prevalence of these enzymes among Escherichia coli the most frequent isolate of Enterobacteriaceae producing Extended-Spectrum β -Lactamase(ESBLs), to differentiate the types of enzymes in these isolates. Total 74(26.2%) Strains of producing ESBLs among the 282 E. coli isolates were isolated from hospitals of Chungcheong area (Daejeon, Chungnam, and Chungbuk) during a 6 month-period from February to July, 2013. 282 E. coli isolates including ESBL shown resistance rates of aztreonam 30.8%, Cefotaxim 30.9%, and Ceftazidime 32.2%, 74 isolates producing ESBLs in E. coli were resistant rates to Aztreonam 58.1%, Cefotaxim 100%, and Ceftazidime 63.5% of β-lactam antibiotics. CTX-M-2 (48 isolates) was the most prevalent type of ESBLs identified. Followed the order of frequency by PER-1 (28 isolates), VEB-1 (26 isolates) and CTX-M-8 (20 isolates), of the 74 isolates, 2 isolates only showed GES-1 in Chungnam province. Accurate identification type of ESBLs would aid in hospital infection control. This would give aid to the physician to prescribe more appropriate antibiotics.

Evaporating Heat Transfer and Pressure Drop of Hydrocarbon Refrigerants in 9.52 and 12.70㎜ Horizontal Tube

Keun-Tae Lee,Ho-Saeng Lee,Choon-Geun Moon,Jae-Dol Kim,Jung-In Yoon 한국태양에너지학회 2004 한국태양에너지학회 학술대회논문집 Vol.- No.-

냉동공조장치의 냉매 가스압력에 의한 열교환기 홴 구동에 관한 실험적 연구

이근태(Keun-Tae Lee),김재돌(Jae-Dol Kim),한인근(In-Geun Han),윤정인(Jung-In Yoon) 한국마린엔지니어링학회 2004 한국마린엔지니어링학회 학술대회 논문집 Vol.2004 No.-

A Refrigeration and air-conditioning industry has been developed in proportion to the development of industry and the improvement of the standard of living. And many studies have been performed for efficiency improvement, economy energy. simplifying facility of the industry. The purpose of this study is the efficiency improvement and economy energy of vapor compression type refrigeration and air conditioning cycle by using fan rotation power which gets from the gas motor driven the pressure of oneself's working medium. The experimental apparatus is composed of a compressor, an expansive valve and each condenser and each evaporator that has the fan driven by electricity and pressure of refrigerant to compare the cycle of exiting electric driving type with the cycle of fan driven by the pressure of refrigerant The test results show that the motor is working by refrigerant and the power consumption of the whole equipment for refrigerant pressure driving type is less than that of the existing electric driving type.

소구경관을 이용한 흡수기의 열ㆍ물질전달 특성에 관한 실험적 연구

이근태(Keun-Tae Lee),이호생(Ho-Saeng Lee),문춘근(Choon-Geun Moon),강기철(Ki-chol Akng),김재돌(Jae-Dol Kim),윤정인(Jung-In Yoon) 대한설비공학회 2005 대한설비공학회 학술발표대회논문집 Vol.2005 No.6

다이오드 레이저와 PhotoMed, Methyl Pheophorbide A, RadachlorinⓇ을 이용한 녹농균에 대한 항균 광역학 요법

Young-Kyu SONG,Keun-Dol YOOK,Ji-Won KIM 대한임상검사과학회 2024 대한임상검사과학회지(KJCLS) Vol.56 No.1

광역학 요법(photodynamic therapy)은 특정 파장의 빛에 의해 활성화되는 광민감제(photosensitizer)를 사용하여 세포내 산소를 활성화시키는 치료 방법으로, 항생제 내성균에 의한 상처 감염의 치료에 유망한 접근 방법이다. 일반적으로 건강한 사람에게 비병원성인 녹농균(Pseudomonas aeruginosa, P. aeruginosa)은 특정 병원성 징후를 보이지 않지만, 피부 손상이나 면역력이 저하된 사람들에서는 패혈증과 같은 심각한 질병을 유발할 수 있다. 항생제는 P. aeruginosa 감염에 대한 전통적인 치료법이나 약물 오용으로 인한 항생제 내성의 증가는 이러한 감염을 관리하는 데 큰 어려움을 준다. 본 연구에서는 P. aeruginosa의 억제제로서 광민감제(PhotoMed, Methyl pheophorbide A, RadachlorinⓇ)와 다이오드 레이저를 이용한 광역학 치료 효과를 조사하는 것을 목표로 했다. P. aeruginosa 현탁액과 광민감제(PhotoMed, Methyl pheophorbide A, RadachlorinⓇ)를 페트리 접시에 접종하여 30분 후 다이오드 레이저를 사용하여 3 J/cm2의 에너지 밀도로 조사했다. 그 결과, P. aeruginosa는 PhotoMed에서 79.65%, Methyl pheophorbide A에서 47.36%, RadachlorinⓇ에서 40.91% 의 사멸률을 보였다. 이번 연구는 P. aeruginosa를 억제하기 위한 가장 효과적인 접근법이 PhotoMed와 다이오드 레이저를 이용한 광역학 치료임을 보여준다. Photodynamic therapy (PDT) activates intracellular oxygen using a photosensitizer activated by light of a specific wavelength and is a potential means of treating wound infections caused by antibiotic-resistant bacteria. Pseudomonas aeruginosa (P. aeruginosa) is typically non-pathogenic in healthy individuals but can induce severe illnesses like sepsis in the immunocompromised. Antibiotics have been conventionally used to treat P. aeruginosa infections, but increasing antibiotic resistance caused by drug misuse poses a growing challenge to the management of these infections. This study aimed to investigate the ability of PDT using photosensitizers (PhotoMed, Methyl pheophorbide A, or RadachlorinⓇ) and a diode laser to inhibit P. aeruginosa. Suspensions of P. aeruginosa and a photosensitizer were inoculated into Petri dishes and incubated for 30 minutes. Samples were then irradiated with the laser at 3 J/cm2, and after incubation, colony areas were measured. P. aeruginosa killing rates were 79.65% for PhotoMed, 47.36% for Methyl pheophorbide A, and 40.91% for RadachlorinⓇ. This study shows that PDT using a diode laser and a photosensitizer constitutes an effective practical therapeutic approach for inhibiting P. aeruginosa.