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Differential Diagnosis of Human Sparganosis Using Multiplex PCR
Hyeong-Kyu Jeon,Kyu-Heon Kim,Woon-Mok Sohn,Keeseon S. Eom 대한기생충학열대의학회 2018 The Korean Journal of Parasitology Vol.56 No.3
Human sparganosis was diagnosed by morphological and genetic analyses in Korea. The complete mitochondrial genomes of Spirometra erinaceieuropaei and S. decipiens isolated in Korea have been recorded. Present study was performed to provide information to diagnose the etiologic agent of sparganosis by multiplex PCR using mitochondrial genome sequences of S. erinaceieuropaei and S. decipiens. In an effort to examine the differential diagnosis of spirometrid tapeworms, multiplex PCR assays were performed on plerocercoid larvae of S. erinaceieuropaei and S. decipiens. The PCR products obtained using species-specific primers were positively detected in all PCR assays on mixture of S. erinaceieuropaei and S. decipiens DNA. S. erinaceieuropaei-specific bands (239 bp and 401 bp) were obtained from all PCR assays using a mixture of S. erinaceieuropaei-specific primers (Se/Sd-1800F and Se-2018R; Se/Sd-7955F and Se-8356R) and S. erinaceieuropaei template DNA. S. decipiens-specific bands (540 bp and 644 bp) were also detected in all PCR assays containing mixtures of S. decipiens-specific primers (Se/Sd-1800F and Sd-2317R; Se/Sd-7955F and Sd-8567R) and S. decipiens template DNA. Sequence analyses on these species-specific bands revealed 100% sequence identity with homologous regions of the mtDNA sequences of S. erinaceieuropaei and S. decipiens. The multiplex PCR assay was useful for differential diagnosis of human sparganosis by detecting different sizes in species-specific bands.
전형규,Hansol Park,Dongmin Lee,최성준,강예슬,BIA MOHAMMED MEBAREK,이상화,Keeseon S. Eom 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.1
This study was undertaken to determine the complete mitochondrial DNA sequence and structure of the mito- chondrial genome of Spirometra ranarum, and to compare it with those of S. erinaceieuropaei and S. decipiens. The aim of this study was to provide information of the species level taxonomy of Spirometra spp. using the mitochondrial ge- nomes of 3 Spirometra tapeworms. The S. ranarum isolate originated from Myanmar. The mitochondrial genome se- quence of S. ranarum was compared with that of S. erinaceieuropaei (GenBank no. KJ599680) and S. decipiens (Gen- Bank no. KJ599679). The complete mtDNA sequence of S. ranarum comprised 13,644 bp. The S. ranarum mt genome contained 36 genes comprising 12 protein-coding genes, 22 tRNAs and 2 rRNAs. The mt genome lacked the atp8 gene, as found for other cestodes. All genes in the S. ranarum mitochondrial genome are transcribed in the same direction and arranged in the same relative position with respect to gene loci as found for S. erinaceieuropaei and S. decipiens mt ge- nomes. The overall nucleotide sequence divergence of 12 protein-coding genes between S. ranarum and S. decipiens differed by 1.5%, and 100% sequence similarity was found in the cox2 and nad6 genes, while the DNA sequence diver- gence of the cox1, nad1, and nad4 genes of S. ranarum and S. decipiens was 2.2%, 2.1%, and 2.6%, respectively.
Mitochondrial DNA Sequence Variability of Spirometra Species in Asian Countries
전형규,Keeseon S. Eom 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.5
Mitochondrial DNA sequence variability of Spirometra erinaceieuropaei in GenBank was observed by reinvestigation of mitochondrial cox1 and cytb sequences. The DNA sequences were analyzed in this study, comprising complete DNA sequences of cox1 (n=239) and cytb (n=213) genes. The 10 complete mitochondrial DNA sequences of Spirometra species were compared with those of Korea, China and Japan. The sequences were analyzed for nucleotide composition, conserved sites, variable sites, singleton sites and parsimony-informative sites. Phylogenetic analyses was done using neighbor joining, maximum parsimony, Bayesian inference and maximum-likelihood on cox1 and cytb sequences of Spirometra species. These polymorphic sites identified 148 (cox1) and 83 (cytb) haplotypes within 239 and 213 isolates from 3 Asian countries. Phylogenetic tree topologies were presented high-level confidence values for the 2 major branches of 2 Spirometra species containing S. erinaceieuropaei and S. decipiens, and S. decipiens sub-clades including all sequences registered as S. erinaceieuropaei in cox1 and cytb genes. These results indicated that mitochondrial haplotypes of S. erinaceieuropaei and S. decipiens were found in the 3 Asian countries.
Hyeong-Kyu Jeon,Hansol Park,Dongmin Lee,Seongjun Choe,Keeseon S. Eom 대한기생충학열대의학회 2018 The Korean Journal of Parasitology Vol.56 No.1
Morphological and molecular characteristics of spirometrid tapeworms, Spirometra decipiens, were studied, which were recovered from a heavily infected stray cat road-killed in Eumseong-gun, Chungcheongbuk-do (Province), the Republic of Korea (=Korea). A total of 134 scolices and many broken immature and mature proglottids of Spirometra tapeworms were collected from the small intestine of the cat. Morphological observations were based on 116 specimens. The scolex was 22.8-32.6 mm (27.4 mm in average) in length and small spoon-shape with 2 distinct bothria. The uterus was coiled 3-4 times, the end of the uterus was ball-shaped, and the vaginal aperture shaped as a crescent moon was closer to the cirrus aperture than to the uterine aperture. PCR amplification and direct sequencing of the cox1 target fragment (377 bp in length and corresponding to positions 769-1,146 bp of the cox1 gene) were performed using total genomic DNA extracted from 134 specimens. The cox1 sequences (377 bp) of the specimens showed 99.0% similarity to the reference sequence of S. decipiens and 89.3% similarity to the reference sequence of S. erinaceieuropaei. In the present study, we report a stray cat heavily infected with S. decipiens identified by mitochondrial cox1 sequence analysis and morphological examinations of the adult worms.
충북 영동군 일부 농촌지역 주민의 장내기생충 감염 실태
엄기선,박남준,한광희 충북대학교 의과대학 충북대학교 의학연구소 1998 忠北醫大學術誌 Vol.8 No.1
연구목적 : 충북 영동군 일부 농촌지역의 기생충 감염상을 파악하고자 하였다 대상 및 방법 : 영동군 상촌면 6개리 주민 300명 중 검사에 응한 190례(남82 및 여180례)에 대하여 셀로판후층도말법과 포르말린 에테르 집란법을 실시하고, 이들 중 181례에 대한 설문 조사를 병행하였다 결과 : 충란 양성율은 2.1%(4/190)로서 간흡층이 0.53%, 메타고니무스흡층이 1.58%이었다. 남자의 감염율은 3.65%(3/82), 여자의 감염율은 0.93%(1/108)이었고 이들은 모두 40, 50대 연령층 이었다. 민물고기를 생식한 경험이 있는 대상자는 18.8% (34/181)이었다. 결론 : 패류매개성 기생충만 발견되었으며 민물고기를 생식하는 습관이 있기 때문인 것으로 파악되었다. Purpose : A survey was performed to observe the present status of helminthic infections in inhabitances of Youngdong-Gun, Chungbuk province. Materials and Methods : Total 190(male:82 female:108) stool specimens were collected and examined by formalin-ether concentration technique and cellophane thick smear. Quetionaire survey was also done with 181 subjects. Results : Total infection rate of 2.1% with two kinds of helminth ova was observed: Clonorchis sinensis 0.53% and Metagonimus sp. 1.58%. All of them were 40-50 years old and the infection rate of male(3.65%) was higher than that of female(0.93%). Questionaire survey revealed 18.5%(34/181) of the subjects had experiences of eating raw freshwater fished. Conclusion : Snail-transmitted parasites were only observed in this area and it seemed to have a close relationship with the eating habit of raw fishes.
New record of two Korean feather mites (Acari: Sarcoptiformes: Astigmata) isolated from water birds
Han, Yeong-Deok,Choe, Seongjun,Eom, Keeseon S.,Min, Gi-Sik The National Institute of Biological Resources 2017 Journal of species research Vol.6 No.no.spc
Two feather mites, Scutomegninia phalacrocoracis Dubinin and Dubinina, 1940 and Ptiloxenus major ($M{\acute{e}}gnin$ and Trouessart, 1884) are reported for the first time in Korea. Specimens of S. phalacrocoracis and P. major were collected from the great cormorant, Phalacrocorax carbo and great crested grebe, Podiceps cristatus, respectively. The genera Scutomegninia Dubinin, 1951 and Ptiloxenus Hull, 1934 are also new reports for South Korea. Here, we provide morphological descriptions and illustrations of these two species. Additionally, we provide partial sequences of the mitochondrial cytochrome c oxidase subunit I (COI) as DNA barcodes.
Seongjun Choe,Dongmin Lee,Hansol Park,Mihyeon Oh,Hyeong-Kyu Jeon,Keeseon S. Eom 대한기생충학열대의학회 2014 The Korean Journal of Parasitology Vol.52 No.5
Surveys on helminthic fauna of the nutria, Myocastor coypus, have seldom been performed in the Republic of Korea. In the present study, we describe Strongyloides myopotami (Secernentea: Strongyloididae) recovered from the small intestine of feral nutrias. Total 10 adult nutrias were captured in a wetland area in Gimhae-si (City), Gyeongsangnam-do (Province) in April 2013. They were transported to our laboratory, euthanized with ether, and necropsied. About 1,300 nematode specimens were recovered from 10 nutrias, and some of them were morphologically observed by light and scanning electron microscopies. They were 3.7-4.7 (4.0±0.36) mm in length, 0.03-0.04 (0.033) mm in width. The worm dimension and other morphological characters, including prominent lips of the vulva, blunted conical tail, straight type of the ovary, and 8-chambered stoma, were all consistent with S. myopotami. This nematode fauna is reported for the first time in Korea.