Complete mitochondrial genome of a forensically important muscid, <i>Hydrotaea chalcogaster</i> (Diptera: Muscidae), with notes on its phylogenetic position

Karagozlu, Mustafa Zafer,Choi, Tae-June,Park, Seong Hwan,Shin, Sang Eon,Kim, Chang-Bae 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.2 No.1

<P><B>Abstract</B></P> <P>Muscidae are a dipteran family which is important for forensic investigations. However, it has received limited attention in forensic entomological experiments as a reason of identification issues. It is hard to identify specimens by morphological methods, especially in developmental stages. Therefore, complete mitochondrial genome sequences can be important tool in forensic entomology for identifying species. In this study we sequenced and analyzed the first complete mitochondrial genome from a forensically important Muscidae species <I>Hydrotaea (=Ophyra) chalcogaster</I> by next-generation sequencing. The mitochondrial genome of the sequenced species is circular molecules of 17,076 bp which have the typical mitochondrial genome complement of 13 protein-coding genes, 22 tRNAs, two ribosomal RNA genes and a control region. Rearrangements of gene positions are identical with the ancestral insect genome. Furthermore, phylogenetic relationships of the family Muscidae were evaluated in regard to mitochondrial protein coding genes. The inferred trees indicate that the Muscidae is a paraphyletic family. These data provide additional information for molecular identification of muscid species.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Hydrotaea chalcogaster</I> has importance for forensic investigations. </LI> <LI> The mitochondrial genome of <I>Hydrotaea chalcogaster</I> sequenced and analyzed. </LI> <LI> This is the second complete mitochondrial genome from the genus <I>Hydrotaea.</I> </LI> <LI> Phylogenetic analysis supports monophyly of the superfamily Muscoidea. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Comparative analyses of the three complete mitochondrial genomes from forensic important beetle genus <i>Dermestes</i> with phylogenetic relationships

Karagozlu, Mustafa Zafer,An, Hyung-eun,Park, Seong Hwan,Shin, Sang Eon,Kim, Chang-Bae Elsevier 2019 Gene Vol.706 No.-

<P><B>Abstract</B></P> <P>Necrophagous <I>Dermestes</I> species have high forensic importance in relation to the estimation of elapsed time since death or death season. To further supplement the genome-level features for related species, the complete mitochondrial genome (mitogenome) of <I>Dermestes</I> species <I>D. essellatocollis</I>, <I>D. frischii</I> and <I>D. coarctatus</I> are amplified, sequenced, annotated, analyzed, and compared with other twelve species of the infraorder Bostrichoidea. The mitochondrial genomes were typical circular molecules with 16,218, 15,873 and 15,873 bp in length, respectively. They included 13 protein coding genes, two rRNAs, and 22 tRNAs, as well as the putative control region. The gene orders and orientations are identical to those of other recorded bostrichiformian species and had the ancestral insect gene composition. Furthermore, phylogenetic analyses based on all the mitochondrial protein coding genes for 13 Bostrichoidea and 16 outgroup taxa were performed using Bayesian and Maximum Likelihood analyses. The inferred trees indicate that the genus <I>Dermestes</I> is monophyletic. The monophyly of infraorder Bostrichiformia is not supported. This study provides genomic data for mitochondrial genome library of the genus <I>Dermestes</I> to investigate evolutionary and systematic studies.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The complete mitochondrial genome is useful for phylogenetic studies. </LI> <LI> This study provides the complete mitochondrial genome of three <I>Dermestes</I> species. </LI> <LI> <I>Dermestes tessellatocollis</I>, <I>D. frischii</I> and <I>D. coarctatus</I> are forensically important. </LI> <LI> Our findings supported the polyphyletic Bostrichiformia hypothesis. </LI> </UL> </P>

Potentiality of Green Fluorescent Protein (GFP) from Aequorea victoria - A Mini Review

Karagozlu, Mustafa Zafer,Kim, Se-Kwon The Korean Society for Marine Biotechnology 2011 한국해양바이오학회지 Vol.5 No.4

Green fluorescent protein (GFP), a very important biological agent that involves shifting the color of bioluminescence from blue to green in luminous coelenterates and to increase the quantum yield of light emission. GFP discovered in medusa, Aequorea victoria is a key factor of various biotechnological and cell biological applications. Beside these applications, GFP of A. victoria is generally stable, which does not require co-factors for activity and can be functionally expressed in different bacterial species. This property of GFPs from A. victoria permits them to be a unique tool to monitor gene expression and protein localization in different organisms. The present review brings out the past milestones and future perspectives on GFPs, with an elaborative reviewing on its applications.

Anti-HIV activity of Novel Synthetic Tri-peptide Conjugated Chitosan Oligosaccharides

Mustafa Zafer KARAGOZLU,Fatih KARADENIZ,Se-Kwon KIM 한국생물공학회 2013 한국생물공학회 학술대회 Vol.2013 No.10

Potentiality of Green Fluorescent Protein (GFP) from Aequorea victoria

Mustafa Zafer Karagozlu,Se-Kwon Kim 한국해양바이오학회 2011 한국해양바이오학회지 Vol.5 No.1

Complete mitochondrial genome of a forensically important muscid, Hydrotaea chalcogaster (Diptera: Muscidae), with notes on its phylogenetic position

Mustafa Zafer Karagozlu,최태선,박성환,신상은,김창배 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4

Muscidae are a dipteran family which is important for forensic investigations. However, it has received limited attention in forensic entomological experiments as a reason of identification issues. It is hard to identify specimens by morphological methods, especially in developmental stages. Therefore, complete mitochondrial genome sequences can be important tool in forensic entomology for identifying species. In this study we sequenced and analyzed the first complete mitochondrial genome from a forensically important Muscidae species Hydrotaea (=Ophyra) chalcogaster by next-generation sequencing. The mitochondrial genome of the sequenced species is circular molecules of 17,076 bp which have the typical mitochondrial genome complement of 13 protein-coding genes, 22 tRNAs, two ribosomal RNA genes and a control region. Rearrangements of gene positions are identical with the ancestral insect genome. Furthermore, phylogenetic relationships of the family Muscidae were evaluated in regard to mitochondrial protein coding genes. The inferred trees indicate that the Muscidae is a paraphyletic family. These data provide additional information for molecular identification of muscid species.

Diethyl-aminoethylation of Low Molecular Weight Chitooligosaccharide Enhances Inducement of Apoptosis in Human Adenocarcinoma Cells

Mustafa Zafer Karagozlu,Fatih Karadeniz,Se Kwon Kim 한국키틴키토산학회 2013 한국키틴키토산학회지 Vol.18 No.1

Chitooligosaccharides are the degraded oligomer of chitosan which have various biological functions. Researchers have proved that chemical modifications of chitooligosaccharides (COS) could enhance their bioactivities. In this study, potential apoptotic activities of modified (diethyl-aminoethly chitoligosaccharide, DEAE-COS) and non-modified COS were evaluated and compared using AGS human gastric cancer cells. DEAE-COS was prepared with chemical synthesized by addition of diethyl-aminoethyl group to COS (< 1 kDa) and confirmed by IR spectra results in comparison to previous study. AGS cell viability was measured after chitosan oligomer treatment for 24 and 48 h. Inhibition of cell proliferation on AGS cells due to DEAE-COS and COS treatment showed by dose dependent manner. Furthermore changes in nuclear morphology and changes in cell morphology were observed by light and fluorescent microscopy. Moreover, protein and gene expressions of important regulators involved in apoptosis pathway such as Caspase-9, Bax, p53 and p21 were examined using RT-PCR and Western blot analysis. In compare analysis, even DEAE-COS and COS inhibited apoptosis on AGS cell, DEAE-COS showed significantly higher apoptotic activity than COS. The present results suggest that diethyl-aminoethly addition to low molecular weight COS enhances its apoptotic activity on AGS human adenocarcinoma cells.

Fasudil Increases the Establishment of Somatic Cell Nuclear Transfer Embryonic Stem Cells in Mouse

So, Seongjun,Karagozlu, Mustafa Zafer,Lee, Yeonmi,Kang, Eunju The Korean Society of Animal Reproduction and Biot 2020 한국동물생명공학회지 Vol.35 No.1

Somatic cell nuclear transfer derived embryonic stem cells (NT-ESCs) have significant advantages in various fields such as genetics, embryology, stem cell science, and regenerative medicine. However, the poor establishment of NT-ESCs hinders various research. Here, we applied fasudil, a Rho-associated kinase (ROCK) inhibitor, to develop somatic cell nuclear transfer (SCNT) embryos and establish NT-ESCs. In the study, MII oocytes were isolated from female B6D2F1 mice and performed SCNT with mouse embryonic fibroblasts (MEFs). The reconstructed NT-oocytes were activated artificially, and cultured to blastocysts in KSOM supplemented with 10 μM fasudil. Further, the blastocysts were seeded on inactivated MEFs in embryonic stem cell medium supplemented with 10 μM fasudil. A total of 26% of embryos formed into blastocysts in the fasudil treated group, while this ratio was 44% in the fasudil free control group. On the other hand, 30% of blastocysts were established NT-ESCs after exposure of fasudil, which was significantly higher than the control group (10%). The results suggest that fasudil reduced blastocyst development after SCNT due to inhibition of 2 cell cleavage while improved the establishment of NT-ESCs through the anti-apoptotic pathway.

Phylogenetic investigations of Lingula anatina among some northwestern Pacific populations, based on mitochondrial DNA cytochrome c oxidase subunit I gene

김성근,Mustafa Zafer Karagozlu,김창배 국립중앙과학관 2017 Journal of Asia-Pacific Biodiversity Vol.10 No.2

In this study, five tailed mussels were collected from the western coastal area of South Korea, and their DNA analyses were assessed by sequencing the partial mitochondrial cytochrome c oxidase subunit I (COI) gene. All DNA sequences were identified as Lingula anatina. These are the first COI record of the L. anatina from South Korea. Furthermore, the COI gene sequences of L. anatina reported from China, Japan, and Hong Kong were retrieved from GenBank, and phylogenetic relationships and genetic distances of the organisms were analyzed. Phylogenetic analysis and genetic distances suggest that the most related population with L. anatina of the present study is the Chinese population. The Japanese population diverged early from a linkage that includes Korean and Chinese populations. The Hong Kong population and one Japanese specimen examined were the most distantly related to other populations. This study provides additional data for phylogenetic study of L. anatina.

Fasudil Increases the Establishment of Somatic Cell Nuclear Transfer Embryonic Stem Cells in Mouse

Seongjun So,Mustafa Zafer Karagozlu,Yeonmi Lee,Eunju Kang 한국동물생명공학회(구 한국동물번식학회) 2020 Journal of Animal Reproduction and Biotechnology Vol.35 No.1

Somatic cell nuclear transfer derived embryonic stem cells (NT-ESCs) have significant advantages in various fields such as genetics, embryology, stem cell science, and regenerative medicine. However, the poor establishment of NT-ESCs hinders various research. Here, we applied fasudil, a Rho-associated kinase (ROCK) inhibitor, to develop somatic cell nuclear transfer (SCNT) embryos and establish NT-ESCs. In the study, MII oocytes were isolated from female B6D2F1 mice and performed SCNT with mouse embryonic fibroblasts (MEFs). The reconstructed NT-oocytes were activated artificially, and cultured to blastocysts in KSOM supplemented with 10 μM fasudil. Further, the blastocysts were seeded on inactivated MEFs in embryonic stem cell medium supplemented with 10 μM fasudil. A total of 26% of embryos formed into blastocysts in the fasudil treated group, while this ratio was 44% in the fasudil free control group. On the other hand, 30% of blastocysts were established NT-ESCs after exposure of fasudil, which was significantly higher than the control group (10%). The results suggest that fasudil reduced blastocyst development after SCNT due to inhibition of 2 cell cleavage while improved the establishment of NT-ESCs through the anti-apoptotic pathway.