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A comparison of dimensional standard of several nickel-titanium rotary files
김기원,조경모,박세희,최기열,Bekir Karabucak,김진우 대한치과보존학회 2014 Restorative Dentistry & Endodontics Vol.39 No.1
Objectives: The aim of this study was to compare the dimensional standard of severalnickel-titanium (Ni-Ti) rotary files and verify the size conformity. Materials andMethods: ProFile (Dentsply Maillefer), RaCe (FKG Dentaire), and TF file (SybronEndo)#25 with a 0.04 and 0.06 taper were investigated, with 10 in each group for a totalof 60 files. Digital images of Ni-Ti files were captured under light microscope (SZX16,Olympus) at 32×. Taper and diameter at D1 to D16 of each files were calculated digitallywith AnalySIS TS Materials (OLYMPUS Soft Imaging Solutions). Differences in taper,the diameter of each level (D1 to D16) at 1 mm interval from (ANSI/ADA) specificationNo. 101 were statistically analyzed using one-way ANOVA and Scheffe’s post–hoc testat 95% confidence level. Results: TF was the only group not conform to the nominaltaper in both tapers (p < 0.05). All groups except 0.06 taper ProFile showed significantdifference from the nominal diameter (p < 0.05). Conclusions: Actual size of Ni-Ti file,especially TF, was different from the manufacturer’s statements.
A comparison of dimensional standard of several nickel-titanium rotary files
Kim, Ki-Won,Cho, Kyung-Mo,Park, Se-Hee,Choi, Ki-Yeol,Karabucak, Bekir,Kim, Jin-Woo The Korean Academy of Conservative Dentistry 2014 Restorative Dentistry & Endodontics Vol.39 No.1
Objectives: The aim of this study was to compare the dimensional standard of several nickel-titanium (Ni-Ti) rotary files and verify the size conformity. Materials and Methods: ProFile (Dentsply Maillefer), RaCe (FKG Dentaire), and TF file (SybronEndo) #25 with a 0.04 and 0.06 taper were investigated, with 10 in each group for a total of 60 files. Digital images of Ni-Ti files were captured under light microscope (SZX16, Olympus) at $32{\times}$. Taper and diameter at $D_1$ to $D_{16}$ of each files were calculated digitally with AnalySIS TS Materials (OLYMPUS Soft Imaging Solutions). Differences in taper, the diameter of each level ($D_1$ to $D_{16}$) at 1 mm interval from (ANSI/ADA) specification No. 101 were statistically analyzed using one-way ANOVA and Scheffe's post-hoc test at 95% confidence level. Results: TF was the only group not conform to the nominal taper in both tapers (p < 0.05). All groups except 0.06 taper ProFile showed significant difference from the nominal diameter (p < 0.05). Conclusions: Actual size of Ni-Ti file, especially TF, was different from the manufacturer's statements.
Ahn, So-Yeon,Kim, Nam-Hoon,Kim, Sunil,Karabucak, Bekir,Kim, Euiseong Elsevier 2018 JOURNAL OF ENDODONTICS - Vol.44 No.4
<P>A mandibular molar with a thick buccal bone plate is a challenging problem in endodontic surgery despite the increase in the success rate of endodontic surgery nowadays. This report describes the application of a surgical template to guide osteotomy and facilitate apex localization in a mandibular molar with a thick buccal bone plate. A 57-year-old woman visited the authors' clinic for pain in tooth 19 and was diagnosed with symptomatic apical periodontitis in this previously treated tooth. Nonsurgical retreatment was performed; however, 2 years later, the patient reported pain in the same tooth. A periapical lesion was confirmed using cone-beam computed tomographic (CBCT) imaging, and endodontic surgery on the mesial root of tooth 19 was planned. After CBCT imaging and cast scan data were transferred to implant surgical planning software, the data were superimposed. In the superimposed model, an anchor pin was designed to target the mesial root apex of tooth 19. The surgical template was then printed using a 3-dimensional printer. Endodontic microsurgery included application of this printed surgical template. A computer-aided design/computer-aided manufacturing (CAD/CAM)-guided surgical template minimized the extent of osteotomy and enabled precise targeting of the apex in this case. There were no postoperative complications. A CAD/CAM-guided surgical template is useful in endodontic surgery for complicated cases.</P>
Biocompatibility of two experimental scaffolds for regenerative endodontics
Dephne Jack Xin Leong,Frank C. Setzer,Martin Trope,Bekir Karabucak 대한치과보존학회 2016 Restorative Dentistry & Endodontics Vol.41 No.2
Objectives: The biocompatibility of two experimental scaffolds for potential use in revascularization or pulp regeneration was evaluated. Materials and Methods: One resilient lyophilized collagen scaffold (COLL), releasing metronidazole and clindamycin, was compared to an experimental injectable poly(lactic-co-glycolic) acid scaffold (PLGA), releasing clindamycin. Human dental pulp stem cells (hDPSCs) were seeded at densities of 1.0 x 104, 2.5 x 104, and 5.0 x 104. The cells were investigated by light microscopy (cell morphology), MTT assay (cell proliferation) and a cytokine (IL-8) ELISA test (biocompatibility). Results: Under microscope, the morphology of cells coincubated for 7 days with the scaffolds appeared healthy with COLL. Cells in contact with PLGA showed signs of degeneration and apoptosis. MTT assay showed that at 5.0 x 104 hDPSCs, COLL demonstrated significantly higher cell proliferation rates than cells in media only (control, p < 0.01) or cells co-incubated with PLGA (p < 0.01). In ELISA test, no significant differences were observed between cells with media only and COLL at 1, 3, and 6 days. Cells incubated with PLGA expressed significantly higher IL-8 than the control at all time points (p < 0.01) and compared to COLL after 1 and 3 days (p < 0.01). Conclusions: The COLL showed superior biocompatibility and thus may be suitable for endodontic regeneration purposes.
Biocompatibility of two experimental scaffolds for regenerative endodontics
Leong, Dephne Jack Xin,Setzer, Frank C.,Trope, Martin,Karabucak, Bekir The Korean Academy of Conservative Dentistry 2016 Restorative Dentistry & Endodontics Vol.41 No.2
Objectives: The biocompatibility of two experimental scaffolds for potential use in revascularization or pulp regeneration was evaluated. Materials and Methods: One resilient lyophilized collagen scaffold (COLL), releasing metronidazole and clindamycin, was compared to an experimental injectable poly(lactic-co-glycolic) acid scaffold (PLGA), releasing clindamycin. Human dental pulp stem cells (hDPSCs) were seeded at densities of $1.0{\times}10^4$, $2.5{\times}10^4$, and $5.0{\times}10^4$. The cells were investigated by light microscopy (cell morphology), MTT assay (cell proliferation) and a cytokine (IL-8) ELISA test (biocompatibility). Results: Under microscope, the morphology of cells coincubated for 7 days with the scaffolds appeared healthy with COLL. Cells in contact with PLGA showed signs of degeneration and apoptosis. MTT assay showed that at $5.0{\times}10^4$ hDPSCs, COLL demonstrated significantly higher cell proliferation rates than cells in media only (control, p < 0.01) or cells co-incubated with PLGA (p < 0.01). In ELISA test, no significant differences were observed between cells with media only and COLL at 1, 3, and 6 days. Cells incubated with PLGA expressed significantly higher IL-8 than the control at all time points (p < 0.01) and compared to COLL after 1 and 3 days (p < 0.01). Conclusions: The COLL showed superior biocompatibility and thus may be suitable for endodontic regeneration purposes.