Intracellular IL-4, IL-10, and IFN-gamma levels of leukemic cells and bone marrow T cells in acute leukemia.

Park, Hun Hee,Kim, Myungshin,Lee, Bong-Hee,Lim, Jihyang,Kim, Yonggoo,Lee, Eun Jung,Min, Woo Sung,Kang, Chang Suk,Kim, Won Il,Shim, Sang In,Han, Kyungja Institute for Clinical Science] 2006 Annals of clinical and laboratory science Vol.36 No.1

<P>The quantitative levels of intracellular cytokines IL-4, IL-10, and IFN-gamma (ie, the number of bound PE-conjugated antibody molecules/cell) of leukemic cells and bone marrow T cells (bmT cells) of acute leukemia patients were analyzed by flow cytometry. One hundred, thirty-one (95 AML, 25 ALL, 11 ABL) patients were studied. The leukemic cell IL-4 level was highest in the monocytic AML group (1735 +/- 1056) and lowest in the dysplastic AML group (960 +/- 545). The IFN-gamma level was highest in the acute promyelocytic leukemia (APL) group (495 +/- 159), and lowest in the ALL group (252 +/- 119). The IL-10 level was not significantly different among the diagnosis groups. In bmT cells, the IL-10 level was highest in the dysplastic AML group (972 +/- 1049) and lowest in the APL group (397 +/- 352). The leukemic cell cytokine levels were lowest and bmT cell cytokine levels were highest in the dysplastic AML group. There were no significant correlations of these cytokine levels with 2-yr survival rate, complete remission (CR) rate, or relapse rate. The cytokine levels of bmT cells at the time of CR became normal and were not different among the diagnosis groups. In summary, leukemic cell and bmT cell cytoplasmic expression profiles of IL-4, IL-10, and IFN-gamma are characteristic for each diagnostic group of acute leukemia patients and the profiles of bmT cells are normal at the time of CR.</P>

인간 복막 중피 세포의 Transforming Growth Factor-β1(TGF-β1) 합성에 관한 연구

한대석,최진희,윤견일,강덕희,임현정,홍영숙 대한신장학회 1999 Kidney Research and Clinical Practice Vol.18 No.3

Objective:to investigate the effect of high glucose and spent peritoneal dialysate on the TGF-β1 synthesis of cultured human peritoneal MC(HPMC); to examine the effect of costimulation with high glucose or dialysate and cytokines, interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α), on transforming growth factor(TGF-β1) synthesis of HPMC. Design:HPMCs were exposed to different concentrations of glucose(30, 60 & 90 mM/L) or spent peritoneal dialysate for 48 hours in the absence or presence of IL-1β(1ng/ml) and TNF-α(1ng/ml). TGF-β1 mRNA expression was assessed by Northern blot analysis and TGF-β1 protein synthesis and release by Western blot analysis with immunoprecipitation. Results:Exposure of MC to high glucose condition(30mM, 60mM & 90mM of D- glucose) induced 2.3-, 3.6- and 4.0-fold increases in TGF-β1 mRNA expression of MC with enhanced TGF-β1 protein synthesis and secretion into the media. Incubation with spent dialysate also significantly increased TGF-β1 mRNA expression & protein pared to control media(P$lt;0.05) Stimulation with IL-1β(1ng/ml) or TNF-α(1ng/ml) significantly increased TGF-β1 mRNA expression after 48 hours above the control level by 2.7-fold and 2.1-fold, respectively. However, TNF-α-induced increase in TGF-β1 mRNA expression was not translated into TGF-β1 protein secretion whereas IL-1β stimulation induced a significant increase in TGF-β1 protein secretion as well as TGF-β1 mRNA expression. Combined stimulation of high glucose or spent dialysate together with IL-1β or TNF-α showed a greater increase in TGF-β1 mRNA expression and protein secretion compared to stimulation with high glucose or spent dialysate alone. Conclusion:Our results clearly show that high glucose concentration of peritoneal dialysate and spent dialysate themselves might be sufficient to stimulate the production of TGF-β1 by peritoneal mesothelial cell. This state of chronic induction of TGF-β1 is further exaggerated in the presence of peritonitis because of stimulatory effect of proinflines, resulting in the augmented TGF-β1 synthesis, thus promoting peritoneal fibrosis.

비만여성들의 C-Reactive Protein과 염증반응지표에 미치는 유산소훈련의 효과

신윤아(Yun A Shin),임강일(Kang Il Lim),석민화(Min Hwa Suk) 한국사회체육학회 2007 한국사회체육학회지 Vol.0 No.30

The purpose of this study was investigate the effect of aerobic exercise on high sensitivity C-reactive protein(hs=CRP), inflammatory markers, and cardiovascular risk factors in obese women. Nineteen obese women(body mass indexs≥25) aged 40 to 53 years were randomly assigned to either a training or control group. The training program consisted of 12 weeks of supervised aerobic exercise(60%VO2R, 60min) three times a week on treadmill. Body weight, BMI, percent of body fat, and waist circumference were significantly decreased after training. Aerobic exercise significantly decreased cardiovascular risk factors and inflammatory markers, including fibrinogen, leptin and IL-6 whereas CRP and adiponectin did not change. The reduction of weight after training was positively associated with the decrease of SBP, TC, TC/HDL-C, TG, CRP, leptin, and IL-6. The change of CRP was positively associated with that of fibrinogen and leptin(p<.05), and also a positive correlation between the changes of leptin and IL-6 was observed(p<.01). As a results, a 12-week aerobic exercise intervention improved cardiovascular risk factors as well as inflammatory markers including fibrinogen, IL-6 and leptin, but had no effect on the modification of CRP despite the improvement of body composition in obese women.

Bog blueberry anthocyanins alleviate photoaging in ultraviolet-B irradiation-induced human dermal fibroblasts

Bae, Ji-Young,Lim, Soon Sung,Kim, Sun Ju,Choi, Jung-Suk,Park, Jinseu,Ju, Sung Mi,Han, Seoung Jun,Kang, Il-Jun,Kang, Young-Hee WILEY-VCH Verlag 2009 Molecular Nutrition & Food Research (Print) Vol.53 No.6

<P>Fruits of bog blueberry (Vaccinium uliginosum L.) are rich in anthocyanins that contribute pigmentation. Anthocyanins have received much attention as agents with potentials preventing chronic diseases. This study investigated the capacity of anthocyanin-rich extract from bog blueberry (ATH-BBe) to inhibit photoaging in UV-B-irradiated human dermal fibroblasts. BBe anthocyanins were detected as cyanidin-3-glucoside, petunidin-3-glucoside, malvidin-3-glucoside, and delphinidin3-glucoside. ATH-BBe attenuated UV-B-induced toxicity accompanying reactive oxygen species (ROS) production and the resultant DNA damage responsible for activation of p53 and Bad. Preincubation of ATH-BBe markedly suppressed collagen degradation via blunting production of collagenolytic matrix metalloproteinases (MMP). Additionally, ATH-BBe enhanced UV-B-downregulated procollagen expression at transcriptional levels. We next attempted to explore whether ATH-BBe mitigated the MMP-promoted collagen degradation through blocking nuclear factor κB (NF-κB) activation and MAPK-signaling cascades. UV-B radiation enhanced nuclear translocation of NF-κB, which was reversed by treatment with ATH-BBe. The UV-B irradiation rapidly activated apoptosis signal-regulating kinase-1 (ASK-1)-signaling cascades of JNK and p38 mitogen-activated protein kinase (p38 MAPK), whereas ATH-BBe hampered phosphorylation of c-Jun, p53, and signal transducers and activators of transcription-1 (STAT-1) linked to these MAPK signaling pathways. ATH-BBe diminished UV-B augmented-release of inflammatory interleukin (IL)-6 and IL-8. These results demonstrate that ATH-BBe dampens UV-B-triggered collagen destruction and inflammatory responses through modulating NF-κB-responsive and MAPK-dependent pathways. Therefore, anthocyanins from edible bog blueberry may be protective against UV-induced skin photoaging.</P>

Effects of Aralia cordata Thunb. on Proteoglycan Release, Type II Collagen Degradation and Matrix Metalloproteinase Activity in Rabbit Articular Cartilage Explants

Baek, Yong-Hyeon,Seo, Byung-Kwan,Lee, Jae-Dong,Huh, Jeong-Eun,Yang, Ha-Ru,Cho, Eun-Mi,Choi, Do-Young,Kim, Deog-Yoon,Cho, Yoon-Je,Kim, Kang-Il,Park, Dong-Suk The Korean Acupuncture Moxibustion Medicine Societ 2005 대한침구의학회지 Vol.33 No.4

Background & Objective: Articular cartilage is a potential target for drugs designed to inhibit the activity of matrix metalloproteinases (MMPs) to stop or slow the destruction of the proteoglycan and collagen in the cartilage extracellular matrix. The purpose of this study was to investigate the effects of Aralia cordata Thunb. in inhibiting the release of glycosaminoglycan (GAG), the degradation of collagen, and MMP activity in rabbit articular cartilage explants. Methods : The cartilage-protective effects of Aralia cordata Thunb. were evaluated by using glycosaminoglycan degradation assay, collagen degradation assay, colorimetric analysis of MMP activity, measurement of lactate dehydrogenase activity and histological analysis in rabbit cartilage explants culture. Results : Interleukin-la (IL-1a) rapidly induced GAG, but collagen was much less readily released from cartilage explants. Aralia cordata Thunb. significantly inhibited GAG and collagen release in a concentration-dependent manner. Aralia cordata Thunb. dose-dependently inhibited MMP-3 and MMP-13 expression and activities from IL-1a-treated cartilage explants cultures when tested at concentrations ranging from 0.02 to 0.2 mg/ml. Aralia cordata Thunb. had no harmful effect on chondrocytes viability or cartilage morphology in cartilage explants. Histological analysis indicated that Aralia cordata Thunb. reduced the degradation of the cartilage matrix compared with that of IL -1a-treated cartilage explants.

Effects of Aralia cordata Thunb. on Proteoglycan Release, Type II Collagen Degradation and Matrix Metalloproteinase Activity in Rabbit Articular Cartilage Explants

Baek, Yong-Hyeon,Seo, Byung-Kwan,Lee, Jae-Dong,Huh, Jeong-Eun,Yang, Ha-Ru,Cho, Eun-Mi,Choi, Do-Young,Kim, Deog-Yoon,Cho, Yoon-Je,Kim, Kang-Il,Park, Dong-Suk The Korean AcupunctureMoxibustion Medicine Society 2005 대한침구의학회지 Vol.22 No.2

Background & Objective: Articular cartilage is a potential target for drugs designed to inhibit the activity of matrix metalloproteinases (MMPs) to stop or slow the destruction of the proteoglycan and collagen in the cartilage extracellular matrix. The purpose of this study was to investigate the effects of Aralia cordata Thunb. in inhibiting the release of glycosaminoglycan (GAG), the degradation of collagen, and MMP activity in rabbit articular cartilage explants. Methods : The cartilage-protective effects of Aralia cordata Thunb. were evaluated by using glycosaminoglycan degradation assay, collagen degradation assay, colorimetric analysis of MMP activity, measurement of lactate dehydrogenase activity and histological analysis in rabbit cartilage explants culture. Results : Interleukin-la (IL-1a) rapidly induced GAG, but collagen was much less readily released from cartilage explants. Aralia cordata Thunb. significantly inhibited GAG and collagen release in a concentration-dependent manner. Aralia cordata Thunb. dose-dependently inhibited MMP-3 and MMP-13 expression and activities from IL-1a-treated cartilage explants cultures when tested at concentrations ranging from 0.02 to 0.2 mg/ml. Aralia cordata Thunb. had no harmful effect on chondrocytes viability or cartilage morphology in cartilage explants. Histological analysis indicated that Aralia cordata Thunb. reduced the degradation of the cartilage matrix compared with that of IL -1a-treated cartilage explants.

탄력섬유성 위황색종과 동반된 혈관무늬망막병증 1례

강일석,성열오 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

혈관무늬망막병증은 시신경 유두주위에서 불규칙하게 방사상으로 뻗어나가는 무늬를 나타내는 비교적 드문 질환으로서 보통 양안성이며 후극부에서 잘 관찰할 수 있다. 혈관무늬망막병증은 흔히 전신질환을 잘 동반하는데 가장 흔히 동반되는 질환으로는 탄력섬유성 위황색종, Paget씨 병, 겸상적혈구빈혈이 있다. 탄력섬유성 위황색종은 피부, 눈, 위장관, 심혈관계 등의 탄력섬유를 침범하는 전신질환이다. 저자들은 탄력섬유성 위황색종으로 진단된 환자에게 동반된 혈관무늬망막병증 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Angioid streaks are rare funus lesions that are irregular, spokelike, curvilinear streaks. Angioid streaks radiate outward from the peripapillary area in all directions. These lesions are usually bilateral, and are characteristically found in the posterior pole. Angioid streaks are often associated with a systemic condition, most frequently pseudoxanthoma elasticum, Paget's disease, and the sickle hemoglobinopathies. Pseudoxanthoma elasticum is a form of systemic elastorrhexis that affects mainly the skin, eyes, gastrointestinal system, and cardiovascular system. This report describes a 26-year-old woman who presented with angioid streaks associated with pseudoxanthoma elasticum.

일부 소방공무원의 음주와 직무스트레스와의 관련성

강경화,이강숙,김석일,맹광호,홍현숙,정춘화 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.4

목적 : 소방공무원의 음주량과 직종별 직무 스트레스에 대해 알아보고 보다 나은 스트레스 관리방안과 문제성 음주예방을 위한 프로그램을 모색하기 위해 시도되었다. 대상 : 2000년 7월부터 8월까지 소방공무원 중 구조대원 97명, 구급대원 97명, 화재진압대원 179명,행정대원 116명, 기타대원 49명을 대상으로 설문지를 이용하여 수집된 자료를 분석하였다. 결과 : 소방공무원은 모든 직종에서 승진 및 임금제도의 스트레스 요인 점수가 가장 높았다. 직종별로 직무 내적 요인, 조직에서의 역할, 대인관계, 조직과 풍토, 직장환경과 스트레스 결과 점수에 있어 유의한 차이를 보였다 과음 주자인 경우 직무 내적요인, 직무 스트레스 수준, 개인적 특성, 대처방식의 점수가 높았으나 유의하지 알았다. 음주량은 직무내적 요인 중 업무 피드백과 음의 관련성을, 직무 스트레스 요인 중 역할 갈등은 음주량을 증가시키는 것으로 나타났다. 결론 소방공무원의 가장 높은 직무 스트레스 요인으로는 승진 및 임금제도인 것으로 나타났다. 음주량은 역할 갈등과 같은 직무 스트레스 요인과 관련이 있었으며, 향후 전향적인 음주행태조사를 통해 직무 스트레스 요인이 음주에 미치는 영향을 파악하기 위한 연구가 필요하다고 생각된다. 따라서 본 연구결과를 기초로 소방공무원의 직무 스트레스 요인에 대한 적절한 관리와 정책이 필요한 것으로 사료된다. Objectives : To establish the relationship between alcohol use and job stress among firemen, and to develop a comprehensive health promotion program including stress management and the prevention of problem drinking. Methods : Data was collected from July to August, 2000. Study subjects included 97 rescue workers, 97 emergency medical personnel, 179 fire extinguishers, 116 administrators and 49 others. Results : The firemen displayed higher scores of stress due to promotion-related pres-sures and salary. We found significant differences in job internal factors, job role, inter-personal relationship, organizational norm, work environment and stress outcome among the various jobs. Job internal factors, job stress factors, personal characteristics, coping skills, and stress outcome were higher in heavy drinkers compared with the light and moderate drinkers. Job internal factors and smoking habit showed a positive correlation with the amount of drinking, although coping skill, exercise frequency and monthly income showed a negative relation with the amount consumed. Conclusion : This study suggested that alcohol drinking is associated with job stress. This suggests that a comprehensive health promotion program including stress management and problem drinking prevention and control should be developed.

치환된 알킬요드화물 또는 크산테이트 화합물과 에틸 아크릴산 또는 메틸 비닐케톤과의 분자간 라디칼 탄소-탄소 결합형성반응

강석구,박영원,박천호,조일환,오원택 성균관대학교 1992 論文集 Vol.42 No.2

생리활성천연물인 lipoxin A, carpenter bee 페로몬, 뇌염모기 페로몬, exobrevicomin 등의 합성의 주요 키탈 중간체들을 치환된 요드화물 또는 크산테이트 화합물과 에틸아크릴산 또는 메틸비닐케톤과의 분자간 라디칼 탄소-탄소 형성 반응에 의해 합성하였다. The chiral building blocks for the synthesis of biologically active natural products such as lipoxin A, carpenter bee pheromone, mosquito ov-iposition pheromone, and exo-brevicomins were synthesized by intermol-ecular radical C-C bond formation of the appropriate substituted iodides or xanthates with ethyl acrylate or methyl vinyl ketone.

포르피린증 환자에서 보인 가역적 자기공명영상소견 1례

강석윤,마효일,곽규환,이상암 대한신경과학회 1998 대한신경과학회지 Vol.16 No.2