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Release of Renal Dipeptidase from Rabbit Renal Proximal Tubules and Its Inhibition by Gentamicin
Kang, Bok-Yun,We, Jeoung-Soon,Choi, Kyong,Lee, Hwanghee-Blaise,Han, Ho-Jae,Park, Haeng-Soon The Pharmaceutical Society of Korea 1999 Archives of Pharmacal Research Vol.22 No.4
Effects of several durgs on rabbit renal proximal tubules were examined for the applicability of renal dipeptidase (RDPase, EC 3. 4. 13. 11) release as a model system to study nephrotoxicity. The proximal tubule prepared by the method of Taub (1990) released RDPase spontaneously in the control experiment which was confirmed by Western blotting. RDPase was also released form cisplatin, lipopolysaccardie (LPS), and indomethacin-treated tubules. Gentamicin inhibited RDPase release in a concentration-dependent manner. This RDPase release system may not be a general model to screen nephrotoxicity but could be a useful source of RDPase purification in a simple and inexpensive way.
Release of Renal Dipeptidase from Rabbit Renal Proximal Tubules and Its Inhibition by Centamicin
Kang, Bok Yun,We, Jeoung Soon,Choi, Kyong,Lee, Hwanghee blaise,Han, Ho Jae,Park, Haeng Soon 전남대학교 약품개발연구소 1999 약품개발연구지 Vol.8 No.1
Effects of several drugs on rabbit renal proximal tubules were examined for the applicability of renal dipeptidase (RDPase, EC 3. 4. 13. 111 release as a model system to study nephrotoxicity. The proximal tubule prepared by the method of Taub (1990) released RDPase spontaneously in the control experiment which was confirmed by Western blotting. RDPase was also released from cisplatin, lipopolysaccharide (LPS), and indomethacin-treated tubules. Gentamicin inhibited RDPase release in a concentration-dependent manner. This RDPase release system may not be a general model to screen nephrotoxicity but could be a useful source of RDPase purification in a simple and inexpensive way.
Release of Renal Dipeptidase from Rabbit Renal Proximal Tubules and Its Inhibition by Gentamicin
Kang, Bok Yun,We, Jeoung Soon,Choi, Kyong,Lee, Hwanghee blaise,Han, Ho Jae,Park, Haeng Soon 전남대학교 약품개발연구소 2000 약품개발연구지 Vol.9 No.1
Effects of several drugs on rabbit renal proximal tubules were examined for the applicability of renal dipeptidase (RDPase, EC 3. 4. 13. 11) release as a model system to study nephrotoxicity. The proximal tubule prepared by the method of Taub (1990) released RDPase spontaneously in the control experiment which was confirmed by Westem blotting. RDPase was also released from cisplatin, lipopolysaccharide (LPS), and indomethacin-treated tubules. Gentamicin inhibited RDPase release in a concentration-dependent manner. This RDPase release system may not be a general model to screen nephrotoxicity but could be a useful source of RDPase purification in a simple and inexpensive way.
인삼이 적혈구세포의 해당과정 및 막 투과도에 미치는 영향
강복순(Kang, Bok-Soon),한경희(Han, Kyung-Hee) 대한생리학회 1983 대한생리학회지 Vol.17 No.2
Red cell glycolytic intermediates, metabolites and metabolic ratios were studied. Glycolytic intermediates were measured in neutralized perchloric acid extracts of red cell suspensions after 3 hr incubation at 37℃ in the presence and absence of saponin. Adenosine triphosphate(ATP), adenosine diphosphate(ADP), pyruvate and lactate were measured by enzymatic procedures involving stoichiometric oxidation or reduction of a pyridine nucleotide. Glucose was determined using glucose oxidase after zinc hydroxide extraction. The redox state was calculated from the lactate dehydrogenase equilibrium. Adenosine triphosphatase activity(ATPase) was measured by determining the amount of phosphate released from ATP by washed erythrocyte membranes(ghost) during 20 min. incubation. Both total hydrolysis and the amount of hydrolysis that occured in the presence of ouabain were measured. The second measurement yields Mg-ATPase and represents nonspecific ATPase activity of the membranes. The difference between total and Mg-ATPase activity can be attributed to Na-K-ATPase. For the measurement of sodium fluxes, human erythrocytes were preincubated in <sup>22</sup>Na for 3 hr at 37℃, washed and suspended in a tracer-free medium. The amount of <sup>22</sup>Na transported out of cells at any time was determined by analysis of supernatant samples taken at various time after addition of the labeled cells to isotope-free medium. The cells and medium were separated and the radioactivity appearing in the medium was measured. From the total radioactivity in the suspension and the radioactivity appearing in the medium at known time, the rate constant for sodium release was computed. The results are summarized as follows: 1) ATP and ATP/ADP were found to increase at every concentration of saponin tested whereas ADP declined at every cone. of saponin. The increase in pyruvate and lactate were observed at every cone, of saponin and thus NAD<sup>+</sup>/NADH computed from pyruvate/lactate also increased. Glucose utilization was stimulated by saponin. 2) Na<sup>+</sup>-K<sup>+</sup>-ATPase activities showed a biphasic response to saponin, first increasing in lower concentration and then decreasing in higher concentration of saponin. 3) The efflux of sodium was significantly increased by saponin in the range of 5 to 10 mg%. The stimulatory effect of saponin on the rate constants for active(ouabain-sensitive) sodium efflux was inhibited by addition of ouabain.
Dae-Weon Lee,Jong Bok Seo,Jae Soon Kang,A-YoungKim,Won Tae Kim,Yeon Ho Je,Kook Jin Lim,Si Hyeock Lee,Young Ho Koh 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10
There are increasing interests in developing methods specifically detecting pathogenic Bursaphelenchus xylophilus. In order to develop a detecting method for B .xylophilus, at first we generated monoclonal antibodies (MAbs) specific to B. xylophilus, discriminating from other pine tree resident nematodes. Among 2304 hybridoma fusions screened. We finally selected a MAb clone, 9F10 and used for further study. To identify the antigenic target of MAb-9F10, we employed several biochemical methods such as SDS-PAGE, 2 dimensional electrophoresis, anion exchange chromatography, and immunoprecipitation to separate and isolate an antigenic target. Proteins from above methods were analyzed via nano-LC-ESI-Q-IT-MS. Peptides of GaLECtin were always detected from several proteomic analyses, suggesting that GaLECtin is the antigenic target of MAb-9F10.
강완복,윤재득,김동수,김문보,김기순,서순덕 대한감염학회 1996 감염 Vol.28 No.4
목 적 : 뇌막염은 뇌막의 염증성 반응으로 여러 가지 병원균에 의해서 올 수 있다. 특히 소아에서는 무균성 뇌막염이 많은 것으로 알려져 있고 이중 대다수가 바이러스에 의한 것이다. 특히 장바이러스가 무균성 뇌막염의 약 85 % 를 차지하고 있다. 1993년도 우리 나라에서는 echovirus 9, 30 에 의한 뇌막염의 전국적인 대유행이 있었고 1994년도에는 echovirus 3 에 의하여 뇌막염이 초래되었다. 이러한 뇌막염의 원인 바이러스는 매년 달라질 수 있으므로 1995년도 봄철에 뇌막염으로 입원하는 환자들의 원인 바이러스를 동정하여 보는 바이다. 방 법 : 1995년도 5월과 6월에 걸쳐 연세대학교 의과대학 세브란스병원 소아과에 입원하여 무균성 뇌막염으로 진단된 환아 19 례 를 대상으로 임상적 관찰 및 바이러스동정을 시행하였다. 가검물은 환아의 뇌척수액과 대변을 동시에 채취하여 배양된 RD 세포와 HEp-2 세포에 접종하여 세포병변을 관찰한 후 enterovirus serum pool 에 의한 중화시험을 하였다. Piore 와 Nicoletta 의 방법에 의하여 세포배양액에서 RNA 를 추출하고 PCR 에 의하여 oligonucleotide 를 합성하였다. 그 후 noncoding RNA PCR 에 의하여 DNA 를 합성하고 전기영동을 거쳐 확인하였다. 결 과 : 1) 임상양상 및 경과는 전형적인 무균성 뇌막염의 양상을 보였다. 2) 총 19 례 중에서 8 례 에서 enterovirus 가 자랐으며, 이 중 5례 에서는 coxsackie B3 가 동정되었으며 3 례 에서는 echovirus 7 이 1 례 에서는 echovirus 3 이 동정되었다. 이중 1 례 에서는 coxsackie B3 와 echovirus7 이 동시에 동정되었다. 3) PCR product도 전형적인 장 바이러스에 합당한 소견을 보였다. 결 론 : 1995년도 봄철에 연세대학교 의과대학 세브란스병원 소아과에 무균성 뇌막염으로 입원한 환자의 원인바이러스는 coxsackie B3 와 echovirus 7 에 의한 감염이었다. Background : Aseptic meningitis is a common illness during childhood. Although the etiologic agent is not always identified, viruses are the usual responsible agents. Enterovirus especially causes approximately 85% of all cases of aseptic meningtis. In 1993, there was a nationwide epidemic of aseptic meningitis by echovirus 9 and 30. And we reported that the cause of aseptic meningitis in 1994 was echovirus 3. This study was done to detect the causative virus of aseptic meningitis in 1995. Methods : To isolate the causative viruses, stool and cerebrospinal fluid specimens from the patients with aseptic meningitis, who were admitted to Severance Hospital in 1995, were collected. Cultured RD cells and HEp-2 cells were inoculated with specimens to see the cytopathic effects. Neutralizing antibody tests using enterovirus serum pool were done on the specimens with the cytopathic effects. RNA was isolated from the cultured supernatants of the infected cells by Piore and Nicoletta method. Oligonucleotide was synthesized by PCR, which was run on polyacrylamide gel after purification with HPLC. After running the DNA produced by using Geneamp RNA PCR Kit, electrophoresis was done. Results : Enterovirus was isolated from 8 out of 19 patients. Among these eight, Coxsackie B3 was isolated in 5 patients. Echovirus 7 was isolated in 3 patients. In one of these patients, Coxsackie B3 and echovirus 7 was isolated simultaneously. Echovirus 20 was isolated in one patient. PCR product from these viruses showed a 154 bp band on gel electrophoresis, which was the same PCR product from echovirus 3 supplied from ATCC. Conclusion : The causative viruses of aseptic meningitis in patients who were admitted to Severance Hospital during the spring season of 1995 were Coxsackie B3 and echovirus 7.