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Prostaglandin F receptor expression in intrauterine tissues of pregnant rats
Elvan Anadol,Halit Kanca,Atiye Seda Yar,Fatma Helvacioğlu,Sevda Menevşe,Engin Ҫalgüner,Deniz Erdoğan 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.1
In this investigation, we studied the expression andlocalization of rat prostaglandin F (FP) receptor in uterinetissues of rats on gestational Days 10, 15, 18, 20, 21, 21.5 andpostpartal Days 1 and 3 using Western blotting analysis,real-time PCR, and immunohistochemistry. A high level ofimmunoreactivity was observed on gestational Days 20, 21,and 21.5 with the most significant signals found on Day 20. FP receptor protein was expressed starting on gestationalDay 15, and a fluctuating unsteady increase was observeduntil delivery. Uterine FP receptor mRNA levels were lowbetween Days 10 and 18 of gestation (p < 0.05). Thetranscript level increased significantly on Day 20 andpeaked on Day 21.5 just before labor (p < 0.05). There wasa positive correlation between FP receptor mRNAexpression and serum estradiol levels (rs = 0.78; p < 0.01)along with serum estradiol/progesterone ratios (rs = 0.79; p < 0.01). In summary, we observed an increase FP receptorexpression in rat uterus with advancing gestation, a markedelevation of expression at term, and a concominant decreaseduring the postpartum period. These findings indicate a rolefor uterine FP receptors in the mediation of uterinecontractility at term.
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Properties and Corrosion Resistance of AISI H13 Hot-Work Tool Steel with Borided B4C Powders
Ali Günen,İsmail Hakki Karahan,Mustafa Serdar Karakaş,Bülent Kurt,Yusuf Kanca,Vedat Veli Çay,Murat Yıldız 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.9
In this study, the surface of AISI H13 steel was borided with powder blends of B4Cand NaBF4using the powder-pack methodat 800, 900 and 1000 °C for 2, 4 and 6 h. The structural and mechanical characteristics of the boride layers formed on thesurface were characterized using scanning electron microscopy, energy dispersive spectroscopy, X-ray diffractometry, 2Dsurface profilometry, microhardness and electrochemical corrosion (3.5 wt% NaCl) tests. The boride layer exhibited a singlephase structure (Fe2B) in samples coated at 800 °C and a dual-phase structure (FeB + Fe2B) at higher boriding temperatures(900 and 1000 °C). The boride layers were compact and crack-free in all boriding conditions. Depending on boridingparameters, the thickness, hardness and average surface roughness (Ra) of the coatings were found to range between 5.81and 102.46 μm, 1635–1915 HV and 0.315–0.650 μm, respectively. The borided AISI H13 steel displayed up to 33.5 timesand 2.4 times higher corrosion resistance than untreated AISI H13 steel and martensitic AISI 431 steel, respectively. Thissuggests potential use of borided AISI H13 steel in the steam turbines and marine applications as an alternative to the morecostly martensitic and duplex stainless steel grades. The corrosion resistance depended on the phase structure (single- ordual-layer), density, thickness and surface roughness of the boride coatings.
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