플라보노이드의 세포 신호전달 조절

이응룡,강근호,강용진,김우열,최혜연,김봉우,정효순,조쌍구 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

Many studies revealed the neuroprotective, cardioprotective, and chemopreventive actions of dietary flavonoids. The plausible mechanistic interpretation of the various effects of flavonoids was concentrated on the anti-oxidant or free radical-scavenging properties of these phytochernicals, both in model systems and under in vivo conditions. While there has been a major focus on the anti-oxidant properties. there is an emerging view that flavonoids and their in vivo metabolites. do not act as conventional hydrogen-donating anti-oxidants. but they may exert regulatory functions in cells through actions at protein kinase or lipid kinase signaling pathways. Flavonoids and more recently their metabolites. have been reported to act at phosphoinositide 3-kinase(PI 3-kinase). Akt/protein kinase B(Akt/PKB), protein kinase C (PKC), mitogen activated protein kinase(MAP kinase), and various tyrosine kinases signaling cascades. Inhibitory or stimulatory actions at these pathways are likely to affect cellular function profoundly by altering the phosphorylation state of target molecules and by modulating gene expression. A clear understanding of the mechanisms of action of flavonoids, either as anti-oxidants or modulators of cellular signaling pathways, and the influence of their metabolism on these properties are key to the evaluation of these potent biomolecules as anti-cancer agents, cardio-protectants, and inhibitors of neurodegeneration.

Seasonal Variations and Associated Factors of Gout Attacks: a Prospective Multicenter Study in Korea

Hyo Jin Choi,Ki Won Moon,Hyun-Ok Kim,Yeon-Ah Lee,Seung-Jae Hong,Ju-Yang Jung,Hyoun-Ah Kim,Chang Hee Suh,하유정,In Je Kim,Jisoo Lee,Eun-Kyoung Park,이승근,Mi Ryoung Seo,Han Joo Baek,최상태,송정수 대한의학회 2020 Journal of Korean medical science Vol.35 No.20

Background: We purposed to evaluate the seasonality and associated factors of the incidence of gout attacks in Korea. Methods: We prospectively enrolled patients with gout attacks who were treated at nine rheumatology clinics between January 2015 and July 2018 and followed them for 1-year. Demographic data, clinical and laboratory features, and meteorological data including seasonality were collected. Results: Two hundred-five patients (men, 94.1%) were enrolled. The proportion of patients with initial gout attacks was 46.8% (n = 96). The median age, body mass index, attack duration, and serum uric acid level at enrollment were 50.0 years, 25.4, 5.0 days, and 7.4 mg/ dL, respectively. Gout attacks were most common during spring (43.4%, P < 0.001) and in March (23.4%, P < 0.001). A similar pattern of seasonality was observed in the group with initial gout attacks. Alcohol was the most common provoking factor (39.0%), particularly during summer (50.0%). The median diurnal temperature change on the day of the attack was highest in the spring (9.8°C), followed by winter (9.3°C), fall (8.6°C), and summer (7.1°C) (P = 0.027). The median change in humidity between the 2 consecutive days (the day before and the day of the attack) was significantly different among the seasons (3.0%, spring; 0.3%, summer; −0.9%, fall; −1.2%, winter; P = 0.015). One hundred twenty-five (61%) patients completed 1-year follow-up (51% in the initial attack group). During the follow-up period, 64 gout flares developed (21 in the initial attack group). No significant seasonal variation in the follow-up flares was found. Conclusion: In this prospective study, the most common season and month of gout attacks in Korea are spring and March, respectively. Alcohol is the most common provoking factor, particularly during summer. Diurnal temperature changes on the day of the attack and humidity changes from the day before the attack to the day of the attack are associated with gout attack in our cohort.

Label/Quencher-Free Detection of Exon Deletion Mutation in Epidermal Growth Factor Receptor Gene Using G-Quadruplex-Inducing DNA Probe

( Hyo Ryoung Kim ),( Il Joon Lee ),( Dong-eun Kim ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.1

Detection of exon 19 deletion mutation in the epidermal growth factor receptor (EGFR) gene, which results in increased and sustained phosphorylation of EGFR, is important for diagnosis and treatment guidelines in non-small-cell lung cancer. Here, we have developed a simple and convenient detection system using the interaction between G-quadruplex and fluorophore thioflavin T (ThT) for discriminating EGFR exon 19 deletion mutant DNA from wild type without a label and quencher. In the presence of exon 19 deletion mutant DNA, the probe DNAs annealed to the target sequences were transformed into G-quadruplex structure. Subsequent intercalation of ThT into the G-quadruplex resulted in a light-up fluorescence signal, which reflects the amount of mutant DNA. Due to stark differences in fluorescence intensity between mutant and wild-type DNA, we suggest that the induced G-quadruplex structure in the probe DNA can report the presence of cancer-causing deletion mutant DNAs with high sensitivity.

Molecular Lines Observed with DRAO 2mm Reciever

Hyo-Ryoung Kim,Chang-Hoon Lee,Hyun-Soo Chung,Seon-Tae Han,Kwanh-Dong Kim 한국천문학회 1995 天文學會報 Vol.20 No.-

Facilitation of Polymerase Chain Reaction with Poly(ethylene glycol)-Engrafted Graphene Oxide Analogous to a Single-Stranded-DNA Binding Protein

Kim, Hyo Ryoung,Baek, Ahruem,Lee, Il Joon,Kim, Dong-Eun American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.49

<P>Polymerase chain reaction (PCR), a versatile DNA amplification method, is a fundamental technology in modern life sciences and molecular diagnostics. After multiple rounds of PCR, however, nonspecific DNA fragments are often produced and the amplification efficiency and fidelity decrease. Here, we demonstrated that poly(ethylene glycol)-engrafted nanosized graphene oxide (PEG-nGO) can significantly improve the PCR specificity and efficiency. PEG-nGO allows the specificity to be maintained even after multiple rounds of PCR, allowing reliable amplification at low annealing temperatures. PEG-nGO decreases the nonspecific annealing of single-stranded DNA (ssDNA), such as primer dimerization and false priming, by adsorbing excess primers. Moreover, PEG-nGO interrupts the reannealing of denatured template DNA by preferentially binding to ssDNA. Thus, PEG-nGO enhances the PCR specificity by preferentially binding to ssDNA without inhibiting DNA polymerase, which is analogous to the role of ssDNA binding proteins.</P>

A Spectral Line Survey of Orion-KL from 124.5 to 138.3 GHz

Hyo-Ryoung Kim,Hyun-Soo Chung,Se-Hyung Cho,Duk-Gyoo Roh,Hyun-Goo Kim,Young-Chol Minh 한국천문학회 1998 天文學會報 Vol.23 No.-

3,3′-Diindolylmethane Inhibits Lipopolysaccharide-Induced Microglial Hyperactivation and Attenuates Brain Inflammation

Kim, Hyo Won,Kim, Jiyoung,Kim, Jaekyoon,Lee, Siyoung,Choi, Bo-Ryoung,Han, Jung-Soo,Lee, Ki Won,Lee, Hyong Joo Oxford University Press 2014 TOXICOLOGICAL SCIENCES Vol.137 No.1

<P>Recent studies have revealed that microglial hyperactivation and neuroinflammation are implicated in development and progression of neurodegenerative diseases. In this study, we examined the beneficial effects of 3,3′-diindolylmethane (DIM) and indole-3-carbinol (I3C), dietary components found in cruciferous vegetables, on brain inflammation. DIM, a major metabolite of I3C, suppressed lipopolysaccharide (LPS)–induced expression of inducible nitric oxide synthase and cyclooxygenase-2 in BV-2 microglia, but I3C did not. DIM, but not I3C, attenuated DNA-binding activity of nuclear factor-κB (NF-κB) and phosphorylation of inhibitor of κB, suggesting that DIM might inhibit microglial hyperactivation by attenuating inflammatory transcription factor NF-κB. In addition, DIM, but not I3C, protected primary cortical neurons from inflammatory toxicity induced by the conditioned media from LPS-stimulated BV-2 microglia, indicating that DIM might attenuate microglial hyperactivation–mediated neuronal death. In an <I>in vivo</I> model of neuroinflammation, DIM suppressed LPS-induced brain inflammation in mouse hippocampus, as determined by the number of Iba-1-positive cells and the mRNA expression of F4/80. Taken together, these results suggest that DIM may have beneficial potential against brain inflammation and neurodegenerative diseases through the negative regulation of the NF-κB signal pathway in microglia.</P>

하드디스크 데이타 I/O 속도 측정용 유틸리티 blockwrite 개발과 응용

김효령(Hyo-Ryoung Kim),송민규(Min-Gyu Song) 한국전자통신학회 2020 한국전자통신학회 논문지 Vol.15 No.6

하드디스크의 속도 성능을 탐색해 볼 수 있는 유틸리티를 개발하였다. 하드디스크에 적용하여 속도 곡선을 자세히 볼 수 있음을 보이고, 이동평균 방법을 적용하면 하드디스크의 실린더 구조와 속도 곡선의 변화를 선명하게 볼 수 있도록 하였다. 확장성을 위해 일반 대용량 스토리지에 적용하였으며, 최근에 새로운 저장 매체로 각광 받고 있는 SSD의 I/O 성능을 측정하였다. 10Gbps급 이상의 속도를 보이는 M.2 NVME를 이용하여, 리눅스 O/S에서 제공하느 cp와 속도 비교 실험을 수행함으로서, 유틸리티의 신뢰성을 검증하였다. In order to investigate the speed profile of data I/O of HDD, we have delveloped an utility program. The application to HDD reveals the detail properties of the speed profile of HDD and the relation between the cylinder structrure of HDD and the velocity profile, also. For the extent application, the experiment of the large volume storage was performed, and the profile of SSD media, which is known as the new rapid media, was measured. The new M.2 NVME SSD, which has the ability of over 10Gbps, we can compare the velocities between cp under linux O/S and the utility, and shows that the preformance of the utility can be reliable.

blockwrite를 이용한 대형 스토리지 성능 측정 및 최적화 연구

김효령(Hyo-Ryoung Kim),송민규(Min-Gyu Song),강용우(Yong-Woo Kang) 한국전자통신학회 2021 한국전자통신학회 논문지 Vol.16 No.6

1.4P 대형 스토리지의 성능을 최적화하기 위하여 청크모드별 특성을 조사하고, I/O 속도면에서 청크 512K 모드를 선택하였다. NVME 스토리지 시스템을 구성하여 대형 스토리지의 데이터 서버 성능 측정에 활용하였다. 1.4P 대형 스토리지의 쓰레드 수에 따른 스루풋의 변화를 측정하여 대형 스토리지 시스템의 특성을 파악하였으며, 스루풋의 최대값은 블록크기 32KB에서 133Gbps 성능을 보임을 확인하였다. GridFTP 방식의 globus-url-copy를 이용하여 데이터 송수신 실험을 수행한 결과, 이 대형 스토리지는 33Gbps의 스루풋을 가지고 있음을 알 수 있었다. In order to optimize the performance of 1.4P large storage, the characteristics of each chunk mode were investigated, and the chunk 512K mode was selected in terms of I/O speed. NVME storage system was configured and used to measure data server performance of large storage. By measuring the change in throughput according to the number of threads of the 1.4P large storage, the characteristics of the large storage system were identified, and it was confirmed that the performance was up to 133Gbps with a block size of 32KB. As a result of data transmission/reception experiment using globus-url-copy of GridFTP, it was found that this large storage has a throughput of 33Gbps.

Regulation of apoptosis by modified naringenin derivatives in human colorectal carcinoma RKO cells

Lee, Eung-Ryoung,Kang, Yong-Jin,Kim, Hyun-Jeong,Choi, Hye-Yeon,Kang, Geun-Ho,Kim, Jung-Hyun,Kim, Bong-Woo,Jeong, Hyo-Soon,Park, Yong-Sun,Cho, Ssang-Goo Wiley Subscription Services, Inc., A Wiley Company 2008 Journal of cellular biochemistry Vol.104 No.1

<P>Flavonoids are micronutrients that are widely detected in foods of plant origin and have been ascribed pharmacological properties. Several biological functions of flavonoids have been thus far identified, whereas there currently exists a lack of evidence to support the relationship between the structure-activity relationship and apoptosis-inducing activity. In an attempt to determine the importance of the OH group or substitution of the 5- or 7-carbon in the diphenylpropane skeleton of flavonoids, we selected 14 different flavonoids with different structures, particularly with regard to the 5- or 7-carbon, and found that naringenin treatment caused a slight decrease in the cell viability of the human colorectal carcinoma RKO cells. Next, in order to characterize the effects of specific substitutions of the 7-carbon of naringenin on apoptosis-regulatory activities, and in an attempt to develop anti-proliferative flavonoid derivatives that would be more effective against colon cancer, we originally synthesized several modified naringenin derivatives (MNDs) including 7-O-benzyl naringenin (KUF-1) and 7-O-(m-metoxybenzyl) naringenin (KUF-2). Treatment with KUF-1 or KUF-2 resulted in significant apoptosis-inducing effects concomitant with losses in mitochondrial membrane potential, caspase activation, intracellular ROS production, and sustained ERK activation. Our data show that KUF-1 or KUF-2 regulate the apoptosis of RKO cells via intracellular ROS production coupled with the concomitant activation of the ERK signaling pathway, thereby implying that hydroxylation or substitution at C7 is critical for the apoptosis-inducing activity of flavonoids. J. Cell. Biochem. 104: 259–273, 2008. © 2007 Wiley-Liss, Inc.</P>