Trichome density of main stem is tightly linked to PepMoV resistance in chili pepper (Capsicum annuum L.)

Kim, Hyun Jung,Han, Jung-Heon,Kim, Seungill,Lee, Heung Ryul,Shin, Jun-Sung,Kim, Jeong-Ho,Cho, Juok,Kim, Young Ho,Lee, Hee Jae,Kim, Byung-Dong,Choi, Doil Springer-Verlag 2011 TAG. Theoretical and applied genetics. Theoretisch Vol.122 No.6

β-cell serotonin production is associated with female sex, old age, and diabetes-free condition

Kim, Yeong Gi,Moon, Joon Ho,Kim, Kyuho,Kim, Hyeongseok,Kim, Juok,Jeong, Ji-Seon,Lee, Junguee,Kang, Shinae,Park, Joon Seong,Kim, Hail Academic Press 2017 Biochemical and biophysical research communication Vol. No.

<P><B>Abstract</B></P> <P>Serotonin is known to be present in pancreatic β-cells and to play several physiological roles, including insulin secretion, β-cell proliferation, and paracrine inhibition of α-cells. However, the serotonin production of different cell lines and islets has not been compared based on age, sex, and diabetes related conditions. Here, we directly compared the serotonin concentrations in βTC and MIN6 cell lines, as well as in islets from mice using ultra-performance liquid chromatography tandem mass spectrometry. The average serotonin concentration was 5–10 ng/mg protein in the islets of male and non-pregnant female mice. The serotonin level was higher in females than males at 8 weeks, although there was no difference at 1 year. Furthermore, we observed serotonin by immunofluorescence staining in the pancreatic tissues of mice and human. Serotonin was detected by immunofluorescence staining in a portion of β-cells from islets of old female mice, but not of male or young female mice. A similar pattern was observed in human pancreas as well. In humans, serotonin production in β-cells was associated with a diabetes-free condition. Thus, serotonin production in β-cells was associated with old age, female sex, and diabetes-free condition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Actual serotonin concentrations in cell lines and islets are compared. </LI> <LI> Serotonin over-production in mouse β-cell is aged and female specific. </LI> <LI> Ovariectomy lowers serotonin concentrations in islets. </LI> <LI> Serotonin over-production in human β-cell is aged, female, and non-diabetes specific. </LI> </UL> </P>

The effect of spiritual well-being on stress coping

Kim, Jungae,Kim, Juok The International Promotion Agency of Culture Tech 2019 International Journal of Advanced Culture Technolo Vol.7 No.3

This study was a cross-sectional descriptive investigation study that analyzed the effects of spiritual well-being on stress coping in twenties. Data collection period was from May 1, 2019 to May 20, and 207 people voluntarily participated in the study. The sub-factors of spiritual well-being consisted of religious well-being and existential well-being. The types of stress coping consist of active coping, passive coping, aggressive coping, evasive coping, and social supportive coping. Data was analyzed using frequency analysis, t-test, Pearson correlation and multiple regression analysis using SPSS 18.0. As a result of the analysis, 90 (43.5%) were male and 117 (56.5%) were female. The 71.0% of the participants had no religion, the Buddhists were 14.5%, other religions were 5.8%, Protestants were 4.3%, Catholics were 2.9%, and Won Buddhists were 1.4%. Active coping in stress coping was correlated with religious well-being (r=-.357, p<0.01) and existential well-being (r=.301, p<0.01) under statistical significance. Religious well-being was significantly higher in males (Mean=2.87, SD=.753) than females (Mean=2.49, SD=.772, p<0.01). Existential well-being was also higher in males (Mean=4.25, SD=.841) than in females (Mean=3.95, SD=.694, p<0.01). The religious well-being was significantly higher than active coping (t=6.183, p<0.01), passive coping (t=-3.595, p<0.01), aggressive coping (t=1.991, p<0.05). Existential well-being was significantly higher than active coping (t=5.339, p<0.01), aggressive coping (t=1.659, p<0.05), evasive coping (t=-3.709, p<0.05) at the statistical significance level. Based on the results, it can be suggested that it was necessary to systematically plan spiritual well-being program as a way to cope with stress by knowing that spiritual well-being positively affects active, aggressive stress coping.

A reference measurement procedure for amino acids in blood using isotope dilution ultra-performance liquid chromatography-tandem mass spectrometry

Kim, Juok,Tran, Thi Thanh Huong,Hong, Seon-Pyo,Jeong, Ji-Seon Elsevier 2017 Journal of chromatography. B, Analytical technolog Vol.1055 No.-

<P><B>Abstract</B></P> <P>We described a reference measurement procedure for amino acid (AA) quantification in blood samples based on deproteinization with 5-sulfosalicylic acid (SSA) and an isotope dilution-ultra performance liquid chromatography-tandem mass spectrometry (LC–MS) method. The serum was deproteinized with 15% v/v SSA and the supernatant was injected directly into the LC–MS system without further processing. Compared with the use of other precipitants and water as a control, five model AAs—valine, isoleucine, leucine, tyrosine, and phenylalanine—in the SSA-treated samples showed ionization enhancement as well as stable background signals without significant ion suppression effects. Five analytes were clearly separated within 3min using gradient elution and ion-pair chromatography of water and acetonitrile containing 0.1% v/v trifluoroacetic acid. The limit of detection range of this method was 2–52fmol, and the RSDs of accuracy and precision from intra- and inter-day assays were within 2.7%. The method was applied to various blood samples including serum, whole blood and plasma, with no reasonable measurement bias revealed. The quantification accuracy of this method was then assessed using commercially available plasma certified reference material (CRM) for AA, and the results agreed well within certified values. We finally applied this method to the determination of candidate serum CRM. The optimized protocol was found to be suitable for the accurate quantification of five AAs in serum, and may satisfactorily serve as a primary method for AA measurement in various blood matrices.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Improvement of measurement sensitivity and ionization stability with 5-sulfosalicylic acid. </LI> <LI> The excellent measurement commutability in various blood matrices such as serum, plasma and whole blood as a reference measurement procedure. </LI> <LI> Assessment of the quantification accuracy of the proposed method using certified reference material. </LI> <LI> Determination of five amino acids in serum with strict estimation of measurement uncertainty using proposed method. </LI> </UL> </P>

학생 스스로 모순을 해결하도록 인도하는 언어기교

김주옥(Kim Juok) 길림성민족사무위원회 2014 중국조선어문 Vol.190 No.-

The effect of spiritual well-being on stress coping

Jungae Kim,Juok Kim 국제문화기술진흥원 2019 International Journal of Advanced Culture Technolo Vol.7 No.3

This study was a cross-sectional descriptive investigation study that analyzed the effects of spiritual wellbeing on stress coping in twenties. Data collection period was from May 1, 2019 to May 20, and 207 people voluntarily participated in the study. The sub-factors of spiritual well–being consisted of religious well-being and existential well-being. The types of stress coping consist of active coping, passive coping, aggressive coping, evasive coping, and social supportive coping. Data was analyzed using frequency analysis, t-test, Pearson correlation and multiple regression analysis using SPSS 18.0. As a result of the analysis, 90 (43.5%) were male and 117 (56.5%) were female. The 71.0% of the participants had no religion, the Buddhists were 14.5%, other religions were 5.8%, Protestants were 4.3%, Catholics were 2.9%, and Won Buddhists were 1.4%. Active coping in stress coping was correlated with religious well-being (r=-.357, p<0.01) and existential well-being (r=.301, p<0.01) under statistical significance. Religious well-being was significantly higher in males (Mean=2.87, SD=.753) than females (Mean=2.49, SD=.772, p<0.01). Existential well-being was also higher in males (Mean=4.25, SD=.841) than in females (Mean=3.95, SD=.694, p<0.01). The religious wellbeing was significantly higher than active coping (t=6.183, p<0.01), passive coping (t=-3.595, p<0.01), aggressive coping (t=1.991, p<0.05). Existential well-being was significantly higher than active coping (t=5.339, p<0.01), aggressive coping (t=1.659, p<0.05), evasive coping (t=-3.709, p<0.05) at the statistical significance level. Based on the results, it can be suggested that it was necessary to systematically plan spiritual well-being program as a way to cope with stress by knowing that spiritual well-being positively affects active, aggressive stress coping.

2030세대의 국민연금의 필요성에 대한 태도와 영향요인

문주옥(Juok Mun),김민정(Minjung Kim) 한국FP학회 2023 Financial Planning Review Vol.16 No.1

건축물 연면적 현황과 도시기본계획의 중심지와의 비교 분석을 통한 물리적 중심지 검증 연구

서주옥(Suh, Juok),김도년(Kim, Donyun),이성창(Lee, Seongchang) 한국도시설계학회 2017 도시설계 : 한국도시설계학회지 Vol.18 No.1

본 연구는 서울의 도시기본계획인 ‘2030서울플랜’이 2014년에 확정ㆍ고시됨에 도시기본계획의 물리적 계획인 중심지계획에 따라 중심지가 재대로 관리ㆍ육성되는지를 검증하고, 중심지 형성요인을 파악하여 향후 계획시 검토방향을 제시하는 것이 목적이다. 중심지는 기존 시가지에 중심지를 지정하는 방식과 신규개발지에 중심지를 지정하는 방식으로 구분할 수 있다. 신규개발 중심지는 상암, 문정을 들 수 있으며 이들은 공공주도 사업으로 조성되어 10~20년 내에 물리적인 변화가 나타났다. 기존 시가지를 중심지로 지정한 경우는 청량리ㆍ왕십리, 창동상계, 미아 등으로 성장가능성 등을 고려하여 지정했음에도 불구하고 물리적인 변화가 나타나지 않았다. 이외에도 도시기본계획상 중심지가 아님에도 자생적으로 성장하여 중심지를 형성한 신사, 서초, 양재, 동대문이 있다. 이들은 대부분 상업용도의 비율이 높은 지역으로 관광객 등이 유동인구가 많아 도시가 자생적으로 변모한 경우이다. 이를 통해 중심지를 형성하는 요인을 살펴보면 신규개발지는 도시계획적 수단이 매우 효과적인 것으로 나타났고, 기존 시가지는 도시계획적인 수단보다는 집객이나 도시활동 등이 중심지 형성에 더 많은 영향을 끼치는 것으로 나타났다. 이와 같이 지역의 성격에 따라 그에 적합한 중심지계획을 수립하여야 할 것이다. According to the confirmation and notification of ‘2030 Seoul Plan’ in 2014, this study aims to examine the current status of the urban centers and build up the framework for future plan based on the factors forming the urban center. The urban centers were categorized based on the realization of plan and purpose of designation. The findings for this study are as followed: newly-developed centers such as Sangam, MunJung have been greatly changed in urban environment for the last 10-20 years by public development projects. In the case of designating the existing city area as the urban center, the physical change did not occur even though the designation was made considering the possibility of growth as Cheongryangri/Wangsinri, Changdong Sanggye and Mia. Though these centers had growth potential, there has no physical changes noticeably. Yangjae, Seocho, Shinsa, Dongdaemun, etc. are not the urban centers of Seoul urban basic planing have experienced substantial growth with high agglomeration of neighborhood commercial facilities and a large floating population. The principal influencing factors to form urban center are urban policy or city planning for newly-developed areas. The growth of urban centers in old city, on the other hand, are influenced by visitor attraction factors, urban activities, etc. The findings suggest that considering the characteristics of urban center is a key factor for effective urban planning.

중소병원 간호사의 의료기관인증제 인식 및 직무스트레스, 조직몰입이 이직의도에 미치는 영향

최인(In Choi),황혜민(Hye Min Hwang),김주옥(Juok Kim) 한국산학기술학회 2017 한국산학기술학회논문지 Vol.18 No.5

본 연구는 중소병원에 근무하는 간호사들의 의료기관인증제 인식, 직무스트레스, 조직몰입과 이직의도를 파악하고이 요인들이 이직의도에 미치는 영향을 확인하고자 시도되었다. 연구 대상은 경기도에 소재한 500병상 이하의 의료기관에 근무하는 간호사 202명이었다. 자료수집을 위한 설문조사는 2015년 12월 1일부터 2016년 3월 30일까지 최근 인증평가를 실시한 5곳의 중소병원에 연구자가 직접 방문하여 기관의 승인 후 간호사들에게 설문조사를 하는 방법으로 실시하였다. 연구결과 중소병원 간호사의 이직의도에 유의한 영향을 주는 일반적 특성은 연령(F=4.68, p<.001), 결혼상태(F=3.92, p<.001), 부서유형(F=3.23, p<.008)이었다. 간호사의 이직의도와 의료기관인증제 인식(r=-.46, p<.001), 조직몰입과는 음의 관계(r=-.35, p<.001), 직무스트레스와는 양의 관계(r=.21, p=.002)로 나타났다. 회귀분석 결과 중소병원 간호사의 이직의도에 유의한 영향을 미친 변수는 조직몰입, 근무유형, 직무스트레스가 유의하였으며 전체 22.0%의 설명력을 가졌다. 본 연구 결과를 통해 의료기관 인증제를 실시하는 중소병원 간호사의 이직의도를 낮추기 위해서 중소병원 관계자는 간호사가 직무에 몰입할 수 있는 환경과 제도적 개선에 힘써야 할 것으로 보이며 직무스트레스의 경감을 위한 방안을 마련해야 할 것이다. 또한 근무유형별 이직의도에 차이가 있는 것으로 나타났으므로 특수파트, 병동, 외래 등의 근무유형별로 차별화된 접근이 필요할 것으로 사료된다. This study examined the awareness of the healthcare accreditation system, job stress, organizational commitment, and the turnover intention to determine the effects of these factors on the turnover intention of small and medium sized hospitals. A total of 202 nurses who worked in hospitals with less than 500 beds located in Gyeonggi-do, participated in this study. Questionnaires were given to the nurses who experienced healthcare accreditation, and data were collected between 1 December 2015 and 30 March 2016. As a result, the factors and general characteristics, influencing the turnover intention were found to be age (F=4.68, p<.001), marital condition (F=3.92, p<.001), and department of work (F=3.23, p<.008). The turnover intention was negatively correlated with the awareness of the healthcare accreditation system (r=-.46, p<.001) and nursing organizational commitment(r=-.35, p<.001), but positively correlated with the job stress (r=.21, p=.002). Regression analysis showed that the turnover intention of nurses from small and medium sized hospitals had 22.0% on the organizational commitment, department type, and job stress. Therefore, to decrease the nurses’ the turnover intention, managers of small and medium sized hospitals need to draw up measures to support job involvement and policy improvement. The results also showed the difference in turnover intention according to the department type. Accordingly, there is a need for a differentiated approach according to the department of work, such as a special unit, ward, and outpatient department.

지리산국립공원 구상나무의 수직적 분포 현황

전영문(Youngmoon Chun),박은희(Eunhui Park),이창수(Changsu Lee),조숙경(Sukgyeong Jo),신명화(Myeonghwa Sin),최영래(Yeongrae Choe),윤주옥(Juok Yoon),김명니(Myeongni Kim),채현진(Hyeonjin Chae),조성봉(Seongbong Jo),정명희(Myeonghui Jeong) 국립공원연구원 2023 국립공원연구지 Vol.14 No.2