http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Bae, Jungdon,Kim, Suk Min,Lee, Sun Bok Springer 2015 Extremophiles Vol.19 No.2
<P>We identified the non-phosphorylated L-rhamnose metabolic pathway (Rha_NMP) genes that are homologous to those in the thermoacidophilic archaeon Thermoplasma acidophilum in the genome of the thermoacidophilic bacterium Sulfobacillus thermosulfidooxidans. However, unlike previously known 2-keto-3-deoxy-L-rhamnonate (L-KDR) dehydrogenase (KDRDH) which belongs to the short chain dehydrogenase/reductase superfamily, the putative KDRDHs in S. thermosulfidooxidans (Sulth_3557) and T. acidophilum (Ta0749) belong to the medium chain dehydrogenase/reductase (MDR) superfamily. We demonstrated that Sulth_3559 and Sulth_3557 proteins from S. thermosulfidooxidans function as L-rhamnose dehydrogenase and KDRDH, respectively. Sulth_3557 protein is an NAD(+)-specific KDRDH with optimal temperature and pH of 50 C and 9.5, respectively. The K m and V max values for L-KDR were 2.0 mM and 12.8 U/mg, respectively. Sulth_3557 also showed weak 2,3-butanediol dehydrogenase activity. Phylogenetic analysis suggests that Sulth_3557 and its homologs form a new subfamily in the MDR superfamily. The results shown in this study imply that thermoacidophilic archaea metabolize L-rhamnose to pyruvate and L-lactate by using the MDR-family KDRDH similarly to that of the thermoacidophilic bacterium S. thermosulfidooxidans.</P>
in vitro Cell Factory for Functional Carbohydrates
Jungdon Bae,Dooil Kim,miri Park,Jung Eun Park,Bo-Hyun Park,Dae-Sil Lee 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1
Thermus caldophilus GK24 (TCA) is an extremothermophile growing at 70℃ ,providing industrially important enzymes. From TCA genome sequence, all of gene sresponsible for carbohydrate biosynthetic network were identified .Among them, 120 enzyme genes have been expressed in E. coli and their reactions were characterized. In this lecture, Thermus-derived Carbohydrate Biosynthetic Network’ will be introduced, particularly focused on in vitro operation of a-glucan network’, glycolysis and pentose-phosphate pathway. 1) ‘a-Glucan Network’ showed to be the practical synthetic methods of various sugars such as trehalose, fructose, phosphosugars, sugar-nucleotides, a-glucan and so on. 2) in vitro Glycolysis using glycolytic enzymes have been operated for synthesizing pyruvate from glucose or glucose-1-phosphate. Accordingly, we could have managed to operate the glycolysis in flask. 3) And one pot reaction containing a-glucan phosphorylase, phospho glucomutase, glucose-6-phosphate isomerase, transaldolase and transketolase could synthesize sedoheptulose-7-phosphate from starch. Overall, ‘in vitro Cell Factory’ utilizing thermostable carbohydrate enzymes turned out to be a practical synthetic model to produce C3-C9 sugars, phosphosugars, sugar-nucleotides and biopolymers.
Park, Miri,Bae, Jungdon,Lee, Dae-Sil John Wiley Sons, Ltd. 2008 Phytotherapy research Vol.22 No.11
<P>Ginger (Zingiber officinale Roscoe) has been used widely as a food spice and an herbal medicine. In particular, its gingerol-related components have been reported to possess antimicrobial and antifungal properties, as well as several pharmaceutical properties. However, the effective ginger constituents that inhibit the growth of oral bacteria associated with periodontitis in the human oral cavity have not been elucidated. This study revealed that the ethanol and n-hexane extracts of ginger exhibited antibacterial activities against three anaerobic Gram-negative bacteria, Porphyromonas gingivalis ATCC 53978, Porphyromonas endodontalis ATCC 35406 and Prevotella intermedia ATCC 25611, causing periodontal diseases. Thereafter, five ginger constituents were isolated by a preparative high-performance liquid chromatographic method from the active silica-gel column chromatography fractions, elucidated their structures by nuclear magnetic resonance spectroscopy and electrospray ionization mass spectrometry and their antibacterial activity evaluated. In conclusion, two highly alkylated gingerols, [10]-gingerol and [12]-gingerol effectively inhibited the growth of these oral pathogens at a minimum inhibitory concentration (MIC) range of 6–30 µg/mL. These ginger compounds also killed the oral pathogens at a minimum bactericidal concentration (MBC) range of 4–20 µg/mL, but not the other ginger compounds 5-acetoxy-[6]-gingerol, 3,5-diacetoxy-[6]-gingerdiol and galanolactone. Copyright © 2008 John Wiley & Sons, Ltd.</P>
Song, Min-Jung,Bae, Jungdon,Lee, Dae-Sil,Kim, Chang-Ho,Kim, Jun-Seok,Kim, Seung-Wook,Hong, Suk-In Elsevier 2006 Journal of bioscience and bioengineering Vol.101 No.2
<P>To date, prodigiosin and its analogues which have been shown to have anticancer, cytotoxic and immunosuppressive activities have been isolated from <I>Serratia, Pseudomonas</I> and <I>Streptomyces</I> species, and chemically synthesized. In a previous study, the red pigment content in <I>Serratia</I> sp. KH-95 was enhanced using a casein-enriched medium. Recently, an integrated bioreactor with an internal adsorbent has been developed to increase the production yield and allow easy recovery of the pigment. Thus, this study focused on both purifying and identifying a single red pigment from several pigments attached to the adsorbent in an integrated bioreactor. The red pigment was extracted directly from the internal adsorbent using acidified methanol and phase separation. Subsequently, it was purified by silica gel chromatography and high performance liquid chromatograph (HPLC). As a result, pure prodigiosin was identified by structural studies as a pigment. Also, this downstream procedure that uses the integrated bioreactor can be applied to the direct production and purification of other prodigiosin analogues and hydrophobic alkaloid compounds from several microorganisms.</P>
Lee, Boo-Ja,Kim, Sung-Kyu,Choi, Soo Bok,Bae, Jungdon,Kim, Ki-Jeong,Kim, Young-Jin,Paek, Kyung-Hee Elsevier 2009 FEBS letters Vol.583 No.13
<P><B>Abstract</B></P><P><I>Capsicum annuum</I> L. <I>Bugang</I> exhibits a hypersensitive response against <I>Tobacco mosaic virus</I> (TMV) P<SUB>0</SUB> infection. The <I>C. annuum</I> <I>UDP-glucosyltransferase 1</I> (<I>CaUGT1</I>) gene was upregulated during resistance response to TMV and by salicylic acid, ethephon, methyl viologen, and sodium nitroprusside treatment. When the gene was downregulated by virus-induced gene silencing, a delayed HR was observed. In addition, free and total SA concentrations in the <I>CaUGT1</I>-downregulated hot pepper were decreased by 52% and 48% compared to that of the control plants, respectively. This suggested that the <I>CaUGT1</I> gene was involved in resistance response against TMV infection by controlling the accumulation of SA.</P>