생약제제인 이공산(異功散)의 Cisplatin 유도 신장독성 보호 및 항산화 효과

Lee, Kyung-Tae,Ahn, Kyoo-Seok,Chang, Sung-Goo,Oh, Soo-Myung,Jung, Jee-Chang 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung-Tae Lee, Kyoo-Seok Ahn¹, Sung-Goo Chang², Soo-Myung Oh²and Jee-Chang Jung²¹College of Pharmacy, ²Oriental Medicine and ³College of Medicine, and East-West Medical Reserch Institute, Kyung Hee University, Seoul, Korea. Preventive and Antioxidative Effects of Crude Drug Preparation(E-kong-san) on Cisplatin Induced Nephrotoxicity. Proceedings of International Symposium on East-West Medicine, Seoul. 201-211, 1999. -Nephroprotective effects of a crude drug-prearation (E-kong-san) were determined from cisplatin on rabbit kidney proximal tubule and human renal cortical cells by MTT assays and sustained glucose consumption on ciplatin-induced human renal cortical tissue. Levels of creatinine and blood ure nitrogen(BUN) in serum after administration of cisplatin(0.75mg/kg,i.p.) to E-kong-san(0.75g/kg/day,p.o.)-pretreated rats were markedly lower compared to those of cisplatin-treated rats. Moreover, the administration of E-kong-san significantly inhibited the loss of body weight of cisplatin injected rts. These findings suggest that E-kong-san on the cisplatin induced nephrotoxicity led us to investigate whether the effect of this water extract was a result of anti-oxidation. E-kong-san showed strong free radical scavenger activities on 1,1-dipheny1-2picrylhydrazil(DPPH) radical and xanthine/xanthine oxidase(XOD) generated superoxide anion radical(O2-). We further studied the effects of E-kong-san on lipid peroxidation in rat liver microsomes induced by enzymatic and nonenzymatic methods. E-kong-san exhibited significant inhibition on both ascorbic acid/Fe2+and ADP/NADPH/Fe3+ induced lipid peroxidation in rat liver microsomes. Based on these results, we suggest that-E-kong-san attenuate the cisplatin induced cytotoxicity and its mechanism can be eplained by antioxidant.

급성기 뇌중풍 환자의 실증(實證) 및 허증(虛證)군 특성비교연구

임정태,김미영,최원우,민인규,정우상,문상관,조기호,김영석,Leem, Jung-Tae,Kim, Mi-Young,Choi, Won-Woo,Min, In-Kyu,Jung, Woo-Sang,Moon, Sang-Kwan,Cho, Ki-Ho,Kim, Young-Suk 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.4

Objectives : This study aimed to evaluate the characteristics of acute stroke patients between excess syndrome and deficiency syndrome groups. Method : We recruited stroke patients from the patients admitted to the Department of Internal Medicine of Kyunghee University Oriental Medical Center, Dongguk University Ilsan Oriental Medical Center, Kyungwon University Songpa Oriental Medical Center and Kyungwon University Incheon Oriental Medical Center from April 2007 to August 2008. We diagnosed acute stroke patients as either excess syndrome or deficiency syndrome and analyzed their characteristics for type of stroke, blood test result. Sasang constitution and lifestyle. Result : We found height, weight, BMI, W/H ratio, hypertension, diabetes, migraine, silent infarction, TG, total lipid, HDL-chol, RBC, Hb, hematocrit, alcohol, smoking and Sasang constitution (Tae-eum, So-yang) were more associated with the excess syndrome group. And we found sea food and Sasang constitution(So-eum) was more associated with the deficiency syndrome group. Conclusion : According to the analysis, we found that the excess syndrome group had more risk factors than the deficiency syndrome group. These results could be utilized in the future as a basis material for Oriental medicine therapy. Further studies will be needed to better understand the differences between excess syndrome and deficiency syndrome groups among acute stroke patients.

맨드라미(Celosia cristata L.) 꽃 메탄올 추출물로부터 용매분획된 분획물의 항산화활성

김현영(Hyun Young Kim),고지연(Jee Yeon Ko),송석보(Seuk Bo Song),김정인(Jung In Kim),서혜인(Hye In Seo),이재생(Jae Saeng Lee),곽도연(Do Yeon Kwak),정태욱(Tae Wook Jung),김기영(Ki Young Kim),오인석(In Seok Oh),정헌상(Heon Sang Jeong),우 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.11

맨드라미꽃의 식품학적 이용가능성을 확인해 보고자 항산화성분 및 항산화활성에 대해 검토하였다. 맨드라미꽃의 항산화성분과 활성은 메탄올 추출물과 순차적 용매분획물에 대해 측정하였으며, 메탄올 추출물과 hexane, chloroform, ethyl acetate, butanol 및 물 분획물의 추출수율은 각각 23.33, 10.27, 20.00, 13.63, 17.55 및 38.54%였다. 맨드라미 꽃 메탄올 추출물의 총 polyphenol, flavonoid 및 tannin 함량은 각각 6.80 ㎎ GAE/g ME, 2.34 ㎎ CE/g ME 및 6.23 ㎎ TAE/g ME였다. 순차적 용매분획물 중 butanol 분획에서 각각 14.92 ㎎ GAE/g SF, 5.44 ㎎ CE/g SF 및 13.38 ㎎ TAE/g SF로 함량이 높았다. 총 proanthocyanidin 함량은 44.72 ㎍ CE/g ME였고 순차적 용매분획물은 각각 42.47, 44.43, 50.03, 49.12 및 41.80 ㎍ CE/g ER로 용매간 유의적인 차이를 보이지 않았다. 맨드라미꽃의 메탄올 추출물의 DPPH 및 ABTS radical 소거활성은 각각 5.24 및 10.70 ㎎ TE/g ME였고 butanol 분획에서 각각 12.53 및 21.09 ㎎ TE/g SF로 활성이 높았다. 메탄올 추출물의 SOD 유사활성은 7.96 unit/mL로 나타났고 hexane, chloroform, ethyl acetate, butanol 및 물 분획물은 각각 4.56, 6.15, 8.07, 12.36 및 5.21 unit/mL로 butanol 분획의 활성이 높았다. 맨드라미꽃 추출물의 항산화성분과 항산화활성 간에 높은 정의 상관관계를 보였고 맨드라미꽃 추출물이 체내 및 식품에서 유익한 도움을 줄 수 있을 것으로 생각된다. The purpose of this study was to evaluate the antioxidant contents and activities of solvent fractions from methanolic extracts of cockscome flowers. The yield of methanolic extracts from cockscome flowers was 23.33%, whereas those of its solvent fractions (hexane, chloroform, ethyl acetate, butanol, and water) were 10.27, 20.00, 13.63, 17.55, and 38.54%, respectively. Total polyphenolic, flavonoid, tannin, and proanthocyanidin contents of methanolic extracts (ME) were 6.80 ㎎ gallic acid equivalent (GAE)/g ME, 2.34 ㎎ catechin equivalent (CE)/g ME, 6.23 ㎎ tannic acid equivalent (TAE)/g ME, and 44.72 ㎍ CE/g ME, respectively. The highest total polyphenolic, flavonoid, and tannin contents of solvent fractions were 14.92 ㎎ GAE/g solvent fraction (SF), 5.44 ㎎ CE/g SF, and 13.38 ㎎ TAE/g SF in the butanol fraction, respectively. The total proanthocyanidin contents were 42.47, 44.43, 50.03, 49.12, and 41.80 ㎍ CE/g SF, respectively. The DPPH and ABTS radical scavenging activities from cockscome flowers were 5.24 and 10.70 ㎎ Trolox equivalent (TE)/g ME, respectively. The highest DPPH and ABTS radical scavenging activities of the solvent fractions were 12.53 and 21.09 ㎎ TE/g SF in the butanol fraction, respectively. SOD-like activities of methanolic extracts from cockscome flowers were 7.96 units/mL, whereas those of its solvent fractions were 4.56, 6.15, 8.07, 12.36, and 5.21 units/mL, respectively. The results of this study show that notable antioxidant activities in cockscome flowers have significant health benefits.

비수리(Lespedeza cuneata G. Don) 메탄올 추출물로부터 분획된 용매분획물의 항산화활성과 α-Glucosidase 저해활성

김현영(Hyun Young Kim),고지연(Jee Yeon Ko),송석보(Seuk Bo Song),김정인(Jung In Kim),서혜인(Hye In Seo),이재생(Jae Saeng Lee),곽도연(Do Yeon Kwak),정태욱(Tae Wook Jung),김기영(Ki Young Kim),오인석(In Seok Oh),정헌상(Heon Sang Jeong),우 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.11

비수리의 식품학적 이용가능성을 확인해 보고자 항산화성분 및 항산화활성, superoxide dismutase 유사활성, α-glucosidase 저해활성 등에 대해 검토하였다. 비수리 메탄올 추출물과 hexane, chloroform, ethyl acetate, butanol 및 물 분획 등 순차적 용매분획물의 총 폴리페놀 함량은 각각 12.44, 3.61, 6.39, 27.11, 20.00 및 9.32 ㎎ GAE/g extract residue(ER)로 나타났으며, 총 플라보노이드 함량은 각각 2.94, 9.92, 7.77, 9.27, 5.11 및 2.66 ㎎ CE/g ER, 총 탄닌 함량은 각각 8.75, 10.04, 7.42, 17.32, 11.65 및 7.61 ㎎ TAE/g ER, 총 프로안토시아니딘의 함량은 346.09, 63.50, 103.76, 288.62, 231.99 및 358.48 ㎍ CE/g ER로 나타났다. 비수리 메탄올 추출물과 hexane, chloroform, ethyl acetate, butanol 및 물 분획물의 DPPH radical 소거활성은 각각 20.62, 5.16, 9.29, 20.80, 20.00 및 20.79 ㎎ TE/g ER, ABTS radical 소거활성은 각각 33.86, 9.24, 17.36, 33.76, 33.49 및 33.86 ㎎ TE/g ER로 나타났다. SOD 유사활성은 각각 4.12, 0.61, 2.01, 9.89, 13.47 및 11.82 unit/mL의 활성을 보여 butanol 분획과 물 분획에서 유의적으로 높은 활성을 나타내었고 α-glucosidase 저해활성은 물 분획 50 및 25 ㎍/mL에서 각각 93.85 및 61.64%로 높은 저해활성을 보이는 것으로 나타났다. 이상의 결과에서 비수리 추출물은 항산화성분 및 항산화활성, SOD 유사활성, α-glucosidase 저해활성을 가지는 물질을 함유한 것으로 보이며, 체내 및 식품에서 활성산소 종 제거에 도움을 줄 수 있을 것으로 보인다. The purpose of this study was to evaluate the antioxidant contents and antioxidant activities of solvent fractions from methanolic extract of sericea lespedeza. To determine the antioxidant compounds in solvent fractions from methanolic extract, total polyphenolic, flavonoid, tannin, and proanthocyanidin contents were measured by spectrophotometric methods. Solvent fractions were evaluated for antioxidative capacity according to DPPH and ABTS radical scavenging activities. Total polyphenolic contents were 12.44, 3.61, 6.39, 27.11, 20.00, and 9.32 ㎎ gallic acid equivalent (GAE)/g extract residue (ER), respectively. Total flavonoid contents were 2.94, 9.92, 7.77, 9.27, 5.11, and 2.66 ㎎ catechin equivalent (CE)/g ER, respectively. Total tannin contents were 8.75, 10.04, 7.42, 17.32, 11.65, and 7.61 ㎎ tannic acid equivalent (TAE)/g ER, respectively. Total proanthocyanidin contents were 346.09, 63.50, 103.76, 288.62, 231.99, and 358.48 ㎍ CE/g ER, respectively. DPPH radical scavenging activities of solvent fractions from methanolic extract of sericea lespedeza were 20.62, 5.16, 9.29, 20.80, 20.00 and 20.79 ㎎ Trolox equivalent (TE)/g ER, and ABTS radical scavenging activities were 33.86, 9.24, 17.36, 33.76, 33.49, and 33.86 ㎎ TE/g ER, respectively. SOD-like activities were 4.12, 0.61, 2.01, 9.89, 13.47, and 11.82 units/mL, and α-glucosidase inhibition activities were 93.85 and 61.64% at concentrations of 50 and 25 ㎍/mL in the water fraction, respectively. The results of this study show that notable antioxidant activities in sericea lespedeza have significant health benefits.

Study of Police Response to Radiation Accidents and Terrorism

Sang Hoon Kim(김상훈),Eun-Tae Park(박은태),Jung Hoon Kim(김정훈) 한국방사선학회 2017 한국방사선학회 논문지 Vol.11 No.7

본 연구는 원자력 발전소가 인접해 있는 부산지역 경찰관들의 원자력 사고와 방사능 테러에 대한 인식과 대처 방안을 파악함으로써, 이와 관련한 정책 수립에 기초자료를 제공하고자 하였다. 부산광역시에 근무하는 221명의 경찰을 대상으로 하였으며, 조직구성, 지휘능력, 여론운영 등으로 설문을 구성하여 재난대응 인식도를 분석하였다. 그 결과, 성별 및 지휘계급에 따른 재난대응 인식은 큰 차이가 없는 것으로 나타났다(p>.05). 연령의 경우 지휘능력에 대한 통계적 차이(p<.05)를 보였으며, 근무경력에 따른 지휘능력도 통계적 차이(p<.05)를 나타냈다. 상관관계 분석 결과, 조직구성에 대한 인식과 지휘능력, 여론운영 3가지 항목이 +0.5 이상으로 정적상관을 나타냈다(p<.01). 전반적으로 경찰 체제에 대해 긍정적인 것으로 나타났으며, 방사선사고 및 테러 발생 시 경찰의 업무를 잘 인식하고 있었다. The purpose of this study is to provide basic data for policy making by understanding preparation plans and awareness of nuclear accidents and radiation terrorism among police officers in the vicinity of nuclear power plants. 221 police officers working in Busan metropolitan city were surveyed and their perception of disaster response was analyzed by organizational structure, command ability, and opinion management. As a result, there was no significant difference in disaster response perception by gender and command class (p>.05). There was statistically significant difference in the ability of command in age and ability according to work experience. As a result of the correlation analysis, there were statistically significant correlations among the three items of organizational structure perception, command ability, and opinion management (+0.5) (p<.01). Overall, it was positive for the police system, and it was well aware of the police work in case of radiation accidents and terrorist attacks. The purpose of this study is to provide basic data for policy making by understanding the coping and awareness of nuclear accidents and radiation terrorism among police officers in the vicinity of nuclear power plants.

Complete chloroplast genome sequence of Capsicum baccatum var. baccatum

Tae-Sung Kim,Jung-Ro Lee,Sebastin Raveendar,Gi-An Lee,Young-Ah Jeon,Ho-Sun Lee,Eun Seong Park,Kyung-Ho Ma,Sok-Young Lee,Jong-Wook Chung 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

The chloroplast (cp) is an organelle with its own genome that encodes a number of cp-specific components. Resequencing technology via next-generation sequencing has recently been successfully applied to cp genome characterization. The field of cp characterization is rapidly growing due to its wide versatility and two complete chloroplast (cp) genome sequences of Capsicum species have been reported. We herein report the complete chloroplast genome sequence of Capsicum baccatum var. baccatum, a wild Capsicum species. The total length of the chloroplast genome is 157,145 bp with 37.7% overall GC content. One pair of inverted repeats, 25,910 bp in length, was separated by a small single-copy region (17,974 bp) and large single-copy region (87,351 bp). This region contains 86 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. Eleven genes contain one or two introns. Pair-wise alignments of cp genome were performed for genome-wide comparison. Analysis revealed a total of 134 simple sequence repeat (SSR) motif and 282 insertions or deletions variants in the C. baccatum var. baccatum cp genome.

Development and characterization of endophyte free tall fescue variety Greenmaster3ho

Sang-Hoon Lee,Ki-Won Lee,Ki-Yong Kim,Hee Jung Ji,Tae Young Hwang,Hyung Soo Park,Hyun Seok Chae 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

In order to improve high persistence and forage quality, through selection of various superior parental varieties for breeding and synthesis of them with new lines, there are ongoing worldwide studies aiming to enhance the quality of tall fescue through a traditional breeding method by selection and hybridization. A new tall fescue variety (Festuca arundinacea Schreb.), named Greenmaster3ho, was developed by the National Institute of Animal Science, Rural Development Administration in Korea from 2010 to 2014. For synthetic seed production of this new variety, five superior clones, 09XFa02, 09XFa03, 09XFa11, 09XFa13, and 09XFa14 were selected and polycrossed. The agronomic growth characteristics and forage production capability of the seeds were studied at Cheonan from 2010, and regional trials were conducted in Cheonan, Hoengseong, Jeju, and Jinju from 2012 to 2014. Greenmaster3ho showed enhanced disease resistance, persistence, and regrowth ability as compared to Fawn. The dry matter yield of Greenmaster 2 was 29% higher (15,119 kg/ha) than that of Fawn. However, the nutritive value of both varieties was similar. This study developed a new tall fescue variety with excellent environmental adaptability, aiming to make a contribution to the vitalization of the Korean grassland industry.

Differences of arthropod community composition between coniferous and deciduous forests

Tae-Sung Kwon,Seung-Jae Jung,Cheol Min Lee,Young-Kyu Park,Joo Han Sung,Young Geun Lee,Young-Seuk Park 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.04

Distribution and abundance of species are influenced by their environmental condition. Among various environmental factors, a vegetation is one of the most influential factors, because it is used as habitats as well as food resources. Arthropods including insects play a important role in forest ecosystems as grazers, scavenger, predators, etc. Deciduous and coniferous forests have different plant community composition, resulting in different environmental condition. In this study, we collected arthropod communities at two different deciduous and coniferous forest stands in the Mountain Gariwang. At each stand, three different sampling methods were used: soil core for soil macroinvertebrate, pitfall trap for wandering insects, and sweeping net for insects in the shrups. Therefore, sampling was conducted at vertical habitats in the forest. The results showed that there were no difference in the number of orders from soil at both coniferous and deciduous stands. However, the abundance was higher at coniferous stand than at deciduous stand. Collembola was the dominant taxa in soil. Meanwhile, there were no statistically significant differences of community composition between coniferous stand and deciduous stands. Multivariate analyses was conducted to characterize differences of communities at different stands as well as vertical distribution in forest.

Differentially expressed proteins between two Korean inbred lines under drought stress at vegetative stage

Sang Gon Kim,Seonghyu Shin,Hwan Hee Bae,Jin-Seok Lee,Jung-Tae Kim,Min Jung Seo,Beom-Young Son,Jeom Ho Lee,Seong-Bum Baek 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Understanding the response of a crop to water deficiency is the first step towards breeding drought-tolerant varieties. In this study, inbred maize (Zea mays L.) lines KS140 and KS141 were subjected to drought stress by withholding water for 10 days at the V5 or V6 leaf stage. Water-deficient plants experienced a decrease in relative leaf water content, stomatal conductance, net CO2 assimilation rate, and water use efficiency compared to well-watered plants. This was accompanied by a decrease in the relative leaf water content that resulted in severe growth retardation in KS140 and KS141. However, leaf chlorophyll content in KS140 was unchanged. To understand the proteome dynamics during the 10-day drought stress in maize leaves, comparative proteome analysis was carried out between the well-watered and water-withheld leaves. Differential expression was observed for 29 protein spots from KS140 and 14 protein spots from KS141, and these were identified using MALDI-TOF mass spectrometry. Among identified proteins, metabolism and stress related proteins were highly were increased by drought stress. This study provides a protein profile of a Korean maize inbred line during drought stress, which will be valuable for future studies of the molecular mechanisms underlying drought resistance and for development of selective breeding markers for drought tolerance in maize.

Nucleotide sequencing of cDNAs from Brassica rapa

Sang Ik Song,Chan Seok Shin,Ji Young Lee,Jong Tae Song,Jung Hun Jin,Kyoung Ha Han,Yang Do Choi 한국응용생명화학회(구 한국농화학회) 1993 한국응용생명화학회 학술발표회 Vol.1993 No.-

To isolate the useful and interesting plant genes in large quantities from Brassica raga, random partial sequencing of leaf and inflorescence cDNA clones of Brassica rapa was carried out. cDNA libraries were made with mRNA prepared from leat and inflorescence organ of Brassica rapa, respectively. Approximately 50% of the cDNA clones contained cDNA insert longer than 300bp. Most of the clones have the poly(A) tail sequences. Partial nucleotide sequences of individual cDNA clone from both organs have been determined and compared with nucleotide: sequences in EMBL and amino acid sequences in PIR data bank. Preliminary results promise a good chance to get useful genes by this attempt.