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( Jung Hun Pak ),( Ju Sung Oh ),( Hye Jeong Kim ),( Mi Jin Kim ),( Hong Kyu Choi ),( Ho Won Jung ),( Kyung Ho Kang ),( Ji Ung Jeong ),( Young Soo Chung ) 한국육종학회 2013 Plant Breeding and Biotechnology Vol.1 No.2
A total of 34 T1 transgenic rice lines overexpressing OgPR1 from wild rice (Oryza grandiglumis) were produced in the previous study. Selection of transgenic plants using hygromycin selection medium was continuously done until T4 generation to find ten homozygous lines. These ten T4 lines were established in the Genetically Modified Organism (GMO) field of NICS (National Institute of Crop Science) in the year of 2007. Phenotypic uniformity and performance was evaluated and compared to the control Dongjin. Two lines were selected for Preliminary Yield Trial (PYT) and Replicated Yield Trial (RYT). Based on morpho-agronomic trait performance, the transgenic plants tended to head later than the control. Culm length was similar to the wild-type Dongjin but the panicle length was relatively reduced. In case of panicle number, transgenic lines showed a little increment compared to wild-type. The shape of grain was nearly the same to wild-type. Yield among lines showed a little difference but was statistically not significant. In terms of physiochemical property of the grain, the transgenic lines showed increased amylose content than the wild-type. When OgPR1-expressing transgenic rice plants were tested against rice blast disease, an enhanced resistance against the disease was observed in the transgenic plants.
Phylogenetic Analysis of Common Millet (Panicum miliaceum L.) using NTS of 5S Ribosomal DNA
Jung Hun Pak,Mi Jin Kim,Hye Jeong Kim,Sang Hyun Shin,Myung-Chul Seo,In-Seok Oh,Ki Young Kim,Tae Wook Jung,Young Soo Chung 한국육종학회 2012 한국육종학회지 Vol.44 No.4
Twenty two common millet (Panicum miliaceum L.) varieties collected from Korea, China and Russia were investigated for their phylogenetic relationship using 5S ribosomal DNA sequences with a hope to provide the basic information on their exact origin. Sequences of 5S rDNA were isolated by PCR. The primers, 5s-rRNA1 and 5s-rRNA2, were designed to isolate the complete NTS. Genomic DNA amplification produced two fragments with different length, 900 bp and 400 bp fragments, confirming the presence of two types of 5S rDNA repeats that differed from each other in the length of the NTS region. Amplified DNAs of 400 bp fragment were subcloned and used for further investigation. The obtained NTS sequences ranged from 200 to 300 bp and homology of sequences among plant materials was much higher than long repeat. CLUSTALW multiple aligment of 5S rDNA sequences from 22 different common millets revealed the clear difference by their origin. And critically different areas with insert or deletion were also confirmed. Those sequence difference seems to be used for discrimination of cultivars from different origin and use as molecular markers for origin identification. In phylogenic tree construction, the clear classification was shown where the genotypes from China and Russia is positioned together and stay away from domestic genotypes.
Jung-Hun Pak,Eun-Sook Chung,Sang-Hyun Shin,Eun-Hee Jeon,Mi-Jin Kim,Hye-Young Lee,Ji-Ung Jeung,Nam-In Hyung,이재헌,Young-Soo Chung 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.2
Oryza grandiglumis Chitinase IVa (OgChitIVa) cDNA encoding a class IV chitinase was cloned from wild rice (Oryza grandiglumis). OgChitIVa cDNA contains an open reading frame of 867 nucleotides encoding 288 amino acid residues with a predicted molecular weight of 30.4 kDa and isoelectric point of 8.48. Deduced amino acid sequences of OgChitIVa include the signal peptide and chitin-binding domain in the N-terminal domain and conserved catalytic domain. OgChitIVa showed significant similarity at the amino acid level with related monocotyledonous rice and maize chitinase, but low similarity with dicotyledoneous chitinase. Southern blot analysis showed that OgChitIVa genes are present as two copies in the wild rice genome. It was shown that RNA expression of Og- ChitIVa was induced by defense/stress signaling chemicals, such as jasmonic acid, salicylic acid, and ethephon or cantharidin and endothall or wounding, and yeast extract. It was demonstrated that overexpression of OgChitIVa in Arabidopsis resulted in mild resistance against the fungal pathogen, Botrytis cinerea, by lowering disease rate and necrosis size. RT-PCR analysis showed that PR-1 and PR-2 RNA expression was induced in the transgenic lines. Here, we suggest that a novel OgChitIVa gene may play a role in signal transduction process in defense response against B. cinerea in plants.
Pak, Jaewoo,Lee, Jung Hun,Pak, Natalie,Pak, Yoon,Park, Kwang Seung,Jeon, Jeong Ho,Jeong, Byeong Chul,Lee, Sang Hee MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.7
<P>Adipose tissue-derived stem cells (ASCs) in the form of stromal vascular fraction (SVF) and cultured expansion have been applied in clinical settings in some countries to treat osteoarthritis (OA) of knees, one of the most common debilitating, incurable disorders. Since the first report of successful cartilage-like tissue regeneration with autologous adipose SVF containing ASCs, there has been a gradual increase in the number of publications confirming such results. Thus far, most of the reports have been limited to treatments of OA of knees. Recently, successful applications of adipose SVF in treating OA of ankles and hips have been reported. In addition, several groups have reported modified methods of applying adipose SVF, such as combining bone marrow stimulation with adipose SVF or adding additional extracellular matrix (ECM) in treating OA. Here, we present an updated, systematic review of clinical effectiveness and safety in treating OA of knees, ankles, and one hip since 2016 using ASCs in the form of adipose SVF or in cultured expansion, along with a description and suggestion of potential biological mechanisms of cartilage regeneration.</P>
Jung Hun Pak,Ha Neui Hong,Quyen Thi Nguyen,Ju Sung Oh,Sang Hyun Shin,Myung-Chul Seo,In-Seok Oh,Ki Young Kim,Tae Wook Jung,Young Soo Chung 한국육종학회 2012 한국육종학회지 Vol.44 No.4
Totally, 26 collections, 17 from Korea and 9 from China, were investigated for their sequences of 5S rDNA, especially the non-transcribed spacers (NTSs). Sequences of 5S rDNA were isolated by PCR using the primers, 5s-rRNA1 and 5s-rRNA2. Genomic DNA PCR produced single amplification of 300, 330, or 350 base pair fragments. Sequence analysis revealed that all inserts contained the part of 5S rDNA gene sequence and the full length of the NTS region. Three different sizes of the fragments were confirmed due to different size of NTS and their length were 300bp, 330bp and 350bp, respectively. Among 17 Korean foxtail millets tested, 14 collections showed single 300bp amplification. Longest fragment amplification, 350bp, was obtained only from the foxtail millet from China origin, even though 2 of them include 300bp fragment. CLUSTALW multiple alignments of 26 foxtail millets clearly revealed 4 areas with certain degree of sequence heterogeneity (region I, II, III, IV). Among 4 boxed areas, foxtail millet genotypes from China have distinct insertion especially in region III. Five of them have extra insertion of sequence and their additional sequences were either 45 or 48 base pair. Three Korean foxtail millets have 32 bp insertion. Other 8 Korean collections have short insert sequences (6 to 8 bp), 3 with 8 bp and 5 with 6 bp. In addition to insert, deletion sequences were also confirmed as major deletion was observed in region II of Chinese collection. The size of deletion was 7 bp long. According to phylogenic tree constructed using MEGA4 program, clear grouping was not revealed. To obtain more convincing results various collections from many countries should be obtained and analyzed to distinguish different germplasm from different origin.
Flanking sequence analysis of soybean transgenic plants with three recombinant cry1Ac genes
Jung Hun Pak,Mi Jin Kim,Hye Jeong Kim,Su Yeong Yun,Young Soo Chung 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Bacillus thuringiensis(Bt) crystal protein (Cry1Ac) genes encode insecticidal δ-endotoxins that are widely used for the development of insect-resistant crops. Common soybean is a crop of economic and nutritious importance in many parts of the world. Korea soybean variety Kwangan was transformed with Bacillus thuringiensis(Bt) crystal protein genes. We transformed three difference Cry1Ac (Cry1Ac and two modified Cry1Ac) genes into Kwangan using highly efficient soybean transformation system. Transgenic plants with Bt crystal protein genes were confirmed for gene introduction and their expression using PCR, real-time PCR, and RT-PCR. We generated 30 independent lines of transgenic soybean plants. Analysis of the flanking sequences isolated by Inverse PCR revealed complex T-DNA insertion patterns and preferential integration of T-DNA into the intergenic spacer region of the soybean genome. We found 5 different intergenic transgenic soybean lines of soybean genome. Currently, the confirmation of stable gene introduction with Bt genes is also performing by southern blot analysis, physiology test, and agronomic characters are investigating.