Endobronchial Neurilemmoma Mimicking a Bronchial Polyp

Ryoung Eun Ko,Ryoung Eun Ko,Ryoung Eun Ko,So Ri Kim,Heung Bum Lee,Yong Chul Lee,Seoung Ju Park 순천향대학교 순천향의학연구소 2015 Journal of Soonchunhyang Medical Science Vol.21 No.2

Neurilemmomas are relatively uncommon, slowly growing tumors which originate from Schwann cells. Intrathoracic neurilemmomas often occur in the chest wall and posterior mediastinum, but endobronchial neurilemmomas are exceedingly rare. These tumors in trachea or bronchus are usually detected by radiologic examinations, mostly computed tomography scan of chest. An 88-year-old man was admitted for management of pneumonia in left lower lobe and parapneumonic effusion. On bronchoscopic examination, there was a small polypoid nodule less than 1 cm in diameter mimicking an endobronchial inflammatory polyp at the bifurcation of the right anterior segmental bronchus and lateral segmental bronchus and under auto-fluorescence imaging, the nodule showed reddish brown area with defined margin. The bronchoscopic biopsy revealed that the bronchial nodule was endobronchial neurilemmoma. This is an interesting case of endobronchial neurilemmoma mimicking a bronchial polyp that is detected incidentally via bronchoscopy.

Induction of Quinone Reductase, an Anticarcinogenic Marker Enzyme, by Extract from Chrysanthemum zawadskii var. latilobum K.

Kim, Ju-Ryoung,Kim, Jung-Hyun,Lim, Hyun-Ae,Jang, Chan-Ho,Kim, Jang-Hoon,Kwon, Chong-Suk,Kim, Young-kyun,Kim, Jong-Sang The Korean Society of Food Science and Nutrition 2005 Preventive Nutrition and Food Science Vol.10 No.4

Induction of NAD(P)H:(quinone-acceptor) oxidoreductase (QR) which promotes obligatory two electron reduction of quinones and prevents their participation in oxidative cycling and thereby the depletion of intracellular glutathione, has been used as a marker for chemopreventive agents. Induction of phase II enzyme is considered to be an important mechanism of cancer prevention. In our previous study, we assessed the quinone reductase QR-inducing activities of 216 kinds of medicinal herb extracts in cultured murine hepatoma cells, BPRc1 and hepalc1c7 cells. Among the 216 herbal extracts tested in that study, extracts from Chrysanthemum zawadskii showed significant induction of QR. In this study, we examined QR-inducing activity of solvent fractions of the herbal extract. The dichloromethane fraction of the herb showed the highest QR induction among the samples fractionated with four kinds of solvents with different polarity. The fraction also significantly induced the activity of glutathione S-transferase (GST), one of the major detoxifying enzymes, at $4{\mu}g/mL\;and\;2{\mu}g/mL$ in hepalc1c7 and BPRc1 cells, respectively. In conclusion, dichloromethane-soluble fraction of Chrysanthemum zawadskii which showed relatively strong induction of detoxifying enzymes merits further study to identify active components and evaluate their potential as cancer preventive agents.

Enhancer of polycomb1 acts on serum response factor to regulate skeletal muscle differentiation.

Kim, Ju-Ryoung,Kee, Hae Jin,Kim, Ji-Young,Joung, Hosouk,Nam, Kwang-Il,Eom, Gwang Hyeon,Choe, Nakwon,Kim, Hyung-Suk,Kim, Jeong Chul,Kook, Hoon,Seo, Sang Beom,Kook, Hyun American Society for Biochemistry and Molecular Bi 2009 The Journal of biological chemistry Vol.284 No.24

<P>Skeletal muscle differentiation is well regulated by a series of transcription factors. We reported previously that enhancer of polycomb1 (Epc1), a chromatin protein, can modulate skeletal muscle differentiation, although the mechanisms of this action have yet to be defined. Here we report that Epc1 recruits both serum response factor (SRF) and p300 to induce skeletal muscle differentiation. Epc1 interacted physically with SRF. Transfection of Epc1 to myoblast cells potentiated the SRF-induced expression of skeletal muscle-specific genes as well as multinucleation. Proximal CArG box in the skeletal alpha-actin promoter was responsible for the synergistic activation of the promoter-luciferase. Epc1 knockdown caused a decrease in the acetylation of histones associated with serum response element (SRE) of the skeletal alpha-actin promoter. The Epc1.SRF complex bound to the SRE, and the knockdown of Epc1 resulted in a decrease in SRF binding to the skeletal alpha-actin promoter. Epc1 recruited histone acetyltransferase activity, which was potentiated by cotransfection with p300 but abolished by si-p300. Epc1 directly bound to p300 in myoblast cells. Epc1+/- mice showed distortion of skeletal alpha-actin, and the isolated myoblasts from the mice had impaired muscle differentiation. These results suggest that Epc1 is required for skeletal muscle differentiation by recruiting both SRF and p300 to the SRE of muscle-specific gene promoters.</P>

The Effects of Kaempferol-Inhibited Autophagy on Osteoclast Formation

Kim, Chang-Ju,Shin, Sang-Hun,Kim, Bok-Joo,Kim, Chul-Hoon,Kim, Jung-Han,Kang, Hae-Mi,Park, Bong-Soo,Kim, In-Ryoung MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.1

<P>Kaempferol, a flavonoid compound, is derived from the rhizome of <I>Kaempferia galanga L</I>., which is used in traditional medicine in Asia. Autophagy has pleiotropic functions that are involved in cell growth, survival, nutrient supply under starvation, defense against pathogens, and antigen presentation. There are many studies dealing with the inhibitory effects of natural flavonoids in bone resorption. However, no studies have explained the relationship between the autophagic and inhibitory processes of osteoclastogenesis by natural flavonoids. The present study was undertaken to investigate the inhibitory effects of osteoclastogenesis through the autophagy inhibition process stimulated by kaempferol in murin macrophage (RAW 264.7) cells. The cytotoxic effect of Kaempferol was investigated by MTT assay. The osteoclast differentiation and autophagic process were confirmed via tartrate-resistant acid phosphatase (TRAP) staining, pit formation assay, western blot, and real-time PCR. Kaempferol controlled the expression of autophagy-related factors and in particular, it strongly inhibited the expression of p62/SQSTM1. In the western blot and real time-PCR analysis, when autophagy was suppressed with the application of 3-Methyladenine (3-MA) only, osteoclast and apoptosis related factors were not significantly affected. However, we found that after cells were treated with kaempferol, these factors inhibited autophagy and activated apoptosis. Therefore, we presume that kaempferol-inhibited autophagy activated apoptosis by degradation of p62/SQSTM1. Further study of the <I>p62/SQSTM1</I> gene as a target in the autophagy mechanism, may help to delineate the potential role of kaempferol in the treatment of bone metabolism disorders.</P>

Expression of Neurotrophic Factors, Tight Junction Proteins, and Cytokines According to the Irritable Bowel Syndrome Subtype and Sex

( Ju Yup Lee ),( Nayoung Kim ),( Ji Hyun Park ),( Ryoung Hee Nam ),( Sun Min Lee ),( Chin-hee Song ),( Geun Kim ),( Hee Young Na ),( Yoon Jin Choi ),( Jin Joo Kim ),( Dong Ho Lee ) 대한소화기 기능성질환·운동학회 2020 Journal of Neurogastroenterology and Motility (JNM Vol.26 No.1

Background/Aims Emerging evidence shows that the mechanism of irritable bowel syndrome (IBS) is associated with neurotrophic factors and tight junction proteins (TJPs). It is known that there are sex differences in the pathophysiology of IBS. The aim of the present study is to determine expression levels of neurotrophic factors, TJPs, and cytokines according to IBS subtype and sex. Methods From 59 IBS (33 IBS-constipation, 21 IBS-diarrhea, and 5 IBS-mixed) and 36 control patients, colonic mucosa mRNA expression levels of transient receptor potential vanilloid-1 (TRPV1), nerve growth factor (NGF), glial cell-derived neurotrophic factor (GDNF), and various TJPs were assessed by real-time polymerase chain reaction. Western blot was performed to determine levels of zonular occludens-1 (ZO-1). Serum levels of cytokines were measured by enzyme-linked immunosorbent assay. Results TRPV1, GDNF, and NGF mRNA levels were significantly increased in those with IBS-constipation compared to those in controls (all P < 0.05). However, they showed no significant difference between those with IBS-diarrhea and controls. Expression level of TRPV1 correlated with that of GDNF (r = 0.741, P < 0.001) and NGF (r = 0.935, P < 0.001). ZO-1 RNA expression levels were lower (P = 0.021) in female IBS-diarrhea than those in controls, although they showed no significant differences between male IBS-diarrhea and controls. Serum IL-1β levels in female IBS were significantly higher than those of male IBS, especially in IBS-constipation (P < 0.001). Conclusion Our results suggest that neurotrophic factors and IL-1β are closely related to IBS-constipation and that decrease of ZO-1 is an important factor in female with IBS-diarrhea.

Regulation of mouse steroidogenesis by WHISTLE and JMJD1C through histone methylation balance

Kim, Sung-Mi,Kim, Ji-Young,Choe, Nak-Won,Cho, Ick-Hyun,Kim, Ju-Ryoung,Kim, Dong-Wook,Seol, Jin-Ee,Lee, Song Eun,Kook, Hoon,Nam, Kwang-Il,Kook, Hyun,Bhak, Young-Yil,Seo, Sang-Beom Oxford University Press 2010 Nucleic acids research Vol.38 No.19

<P>The dynamic exchange of histone lysine methylation status by histone methyltransferases and demethylases has been previously implicated as an important factor in chromatin structure and transcriptional regulation. Using immunoaffinity TAP analysis, we purified the WHISTLE-interacting protein complexes, which include the heat shock protein HSP90α and the jumonji C-domain harboring the histone demethylase JMJD1C. In this study, we demonstrate that JMJD1C specifically demethylates histone H3K9 mono- and di-methylation, and mediates transcriptional activation. We also provide evidence suggesting that both WHISTLE and JMJD1C performs functions in the development of mouse testes by regulating the expression of the steroidogenesis marker, <I>p450c17</I>, via SF-1-mediated transcription. Furthermore, we demonstrate that WHISTLE is recruited to the <I>p450c17</I> promoter via SF-1 and represses the transcription of prepubertal stages of steroidogenesis, after which JMJD1C replaces WHISTLE and activates the expression of target genes via SF-1-mediated interactions. Our results demonstrate that the histone methylation balance mediated by HMTase WHISTLE and demethylase JMJD1C perform a transcriptional regulatory function in mouse testis development.</P>

Masticator space abscess in a 47-day-old infant

Kim, Eun-Hee,Jeon, Ju-Hee,Shim, Yoon-Hee,Lee, Kyu-Seok,Kim, So-Young,Kim, Eun-Ryoung The Korean Pediatric Society 2011 Clinical and Experimental Pediatrics (CEP) Vol.54 No.8

A 47-day-old male infant presented with fever, poor oral intake, irritability, and right-sided bluish buccal swelling. Contrast-enhanced computed tomography of the neck showed a round mass lesion of about $2.0{\times}1.5cm$ that suggested abscess formation in the right masticator space. Ultrasound-guided extraoral aspiration of the abscess at the right masseter muscle was successful. Staphylococcus aureus was identified in the culture from the aspirated pus and blood. Appropriate antibiotics were given and the patient recovered. The patient underwent follow-up ultrasonography that showed an improved state of the previously observed right masseter muscle swelling at about 1 month after hospital discharge. A masticator space abscess usually originates from an odontogenic infection in adults. We report a case of masticator space abscess in a 47-day-old infant in whom septicemia without odontogenic infection was suspected.

Studies on Constituents of Kotean Basidiomycetes (L) Antitmor Components Extracted from Cultured Mycelia of Several Basidiomycetes

Kim, Byong-Kak,Choi, Eung-Chil,Chung, Kyeong-Soo,Park, Hee-Ju,Kim, Hye-Ryoung,Kim, Yang-Sup,Park, Yong-Hwan,Shim, Mi-Ja The Pharmaceutical Society of Korea 1983 Archives of Pharmacal Research Vol.6 No.2

To find anititumor metabolites in Korean basidiomycetes, the shake-cultured mycelia of eight of the higher fungi were extracted with hot water and the extracts, after being partially purified, were subjected to in vivo antitumor test. When administered i. p. at the dose of 30mg/kg/day for ten consecutive days into the female ICR mice, which had been implanted with $1{\times}10^{6}$ / cells of sarcoma 180 twenty four hours before the first injection, the extracts of Agaricus campestris, Lyophyllum decastes, Lyophyllum ulmarium, Armillaria Tabescence and Calvatia exipuliformis respectively showed inhibition ratios of 64.1%, 65.45, 60.-%, 53.0 and 49.3%. These five species were selected for further study, whereas the extracts of Phallus impudicus, Coprinus comatus and Pholiota squarrosa whih showed the inhibition ratios of 31.2%, 33.5% and 19.0% were discontinued.

[P10-145] Induction of Apoptosis by Sesquiterpenes Isolated from Inula helenium in Mouse Hepatoma Cells

Ju Ryoung Kim,Hyun Ae Lim,Chan Ho Jang,Jang Hoon Kim,Jong Sang Kim,Young Kyoon Kim 한국식품영양과학회 2005 한국식품영양과학회 학술대회발표집 Vol.2005 No.10

High-capacity nanostructured manganese dioxide cathode for rechargeable magnesium ion batteries

Kim, Ju-Sik,Chang, Won-Seok,Kim, Ryoung-Hee,Kim, Dong-Young,Han, Dong-Wook,Lee, Kyu-Hyoung,Lee, Seok-Soo,Doo, Seok-Gwang Elsevier 2015 Journal of Power Sources Vol.273 No.-

<P><B>Abstract</B></P> <P>Nanostructured λ-MnO<SUB>2</SUB> and α-MnO<SUB>2</SUB> are investigated for use in rechargeable Mg ion battery (MIB) cathodes. In order to prepare nanosized particles, the manganese dioxides are prepared by the acid treatment of spinel MgMn<SUB>2</SUB>O<SUB>4</SUB> synthesized using the Pechini method. X-ray diffraction analysis indicates that the resulting MnO<SUB>2</SUB> consists of multiple phases, λ-MnO<SUB>2</SUB>, α-MnO<SUB>2</SUB>, and β-MnO<SUB>2</SUB>, depending on the leaching time in acid solution. Upon the first charge–discharge cycle in acetonitrile electrolyte, the λ-MnO<SUB>2</SUB> based electrode shows larger reversible capacity of ∼330 mAh g<SUP>−1</SUP> compared to an electrode containing a large amount of α-MnO<SUB>2</SUB>. This enhanced capacity is associated with the facile charge-transfer reaction of Mg ions at the MnO<SUB>2</SUB>/electrolyte interfaces. The capacity fading of MnO<SUB>2</SUB> in different electrolytes is also discussed in terms of the formation of a surface layer at the electrode/electrolyte interface during the charging process.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Nanostructured λ-MnO<SUB>2</SUB> and α-MnO<SUB>2</SUB> were prepared by the acid treatment of MgMn<SUB>2</SUB>O<SUB>4</SUB>. </LI> <LI> λ-MnO<SUB>2</SUB> shows higher capacity for Mg insertion than α-MnO<SUB>2</SUB>. </LI> <LI> The improved performance of λ-MnO<SUB>2</SUB> is caused by the facile interfacial reaction. </LI> <LI> We explain the capacity fading in terms of the formation of a surface film. </LI> </UL> </P>