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Methylation이 心筋細胞膜에서의 ATP에 의한 Calcium Transport에 미치는 影響
金柱炳,昔廷鎬,李載欣 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2
For the purpose of studying the properties of calcium pump, the effect of methy lation on the ATP-induced Ca-transport in the isolated porcine sarcolemmal vesicles which were loaded with high KCI was investigated. 1) Ca-transport by ATP was increased significantly, compared to control sarcolemmal vesicles. 2) Ca-transport of the vesicles preincubated in the methylation medium without PM II was increased by ATP, but less than control sarcolemmal vesicles. 3) Ca-transport of the vesicles preincubated in the methylation medium with PM II was decreased by ATP in the KCl medium, but increased in NaCI medium. But Ca-transport by ATP in the methylated vesicles in the reaction medium (NaCI and KC1) was significantly decreased, compared with Ca-transport by ATP in the nonmethylated vesicles. From the above results, it was suggested that methylation of membrane protein by PM II can change the membrane properties or the activity of calcium ATPase transporting calcium.