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Microwave-assisted Approach for the Rapid Enzymatic Digestion of Rapeseed Meal
Ju-Fang Li,Fang Wei,Lu-Lu Guo,Gang-You Yuan,Feng-Hong Huang,Mu-Lan Jiang,Yuan-Di Zhao,Xu-Yan Dong,Guang-Ming Li,Hong Chen 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.2
This study demonstrates the use of a new microwave-assisted approach for accelerating the enzymatic digestion of rapeseed meal. The effects of different microwave parameters, such as the time, temperature, and power level, on the degree of hydrolysis (DH) were investigated by using response surface methodology (RSM). The maximum predicted DH value (10.2%) was in good agreement with the value obtained experimentally using an alkaline protease, which was 12.57% under optimal conditions. In only 7 min, the microwave-assisted method achieved a DH value similar to that obtained by the conventional enzymatic digestion method (4 hr). Therefore,this new technique for rapid enzymatic digestion will improve the application of rapeseed meal in the preparation of protein hydrolysates for use in food and feed.
Oxidation behavior of YAG-ZrB2 ceramics prepared by spark plasma sintering
Jie-Guang Song,Da-Ming Du,Yin-Yan Ju,Yang-Liang Li,Shi-Bin Li,Gang-Chang Ji 한양대학교 세라믹연구소 2010 Journal of Ceramic Processing Research Vol.11 No.4
ZrB2 and YAG are widely applied because of some excellent properties, but ZrB2 is easily oxidized in high-temperature air. To make ZrB2 ceramics have better oxidation resistance, high-density YAG-ZrB2 ceramics were prepared. The oxidation behavior of YAG-ZrB2 ceramics is investigated at different temperatures. The results indicate that the enhanced weight change differs according to the temperature range, slightly in the temperature 900-1000oC, sharply from 1100-1300 and smoothly above 1300oC. The weight change rate of YAG-ZrB2 ceramics is increased by prolonging the oxidation time, but the weight change rate of YAG-ZrB2 ceramics is gradually reduced. The oxidation layer is porous as compared to the YAG-ZrB2 ceramics layer.
Zhang, Li-Ying,Yuan, You-Qing,Zhou, Dong-Ming,Wang, Zi-Yan,Ju, Song-Guang,Sun, Yu,Li, Jun,Fu, Jin-Xiang Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.1
In this investigation, global DNA methylation patterns and the specific methylation status of 5 genes were studied in DNA from peripheral blood (PB) and impact on progression free survival (PFS) and overall-survival (OS) in patients with de novo or relapsed acute myeloid leukemia (AML) treated with decitabine-based regimens waas assessed. DNA was isolated from PB samples at the time of -1, 1, and 7 days of chemotherapy. Global methylation was determined by ELISA, and the CpG island DNA methylation profile of 5 genes using a DNA methylation PCR system. Our data demonstrated that patients with a high level of 5-mC had a poor prognosis after demethylation therapy and those who have low levels of 5-mC in PB achieved higher CR and better SO, but there was no significant correlation found between the 5-mC levels and other clinical features before treatment except the disease status. Higher methylation status of Sox2 and Oct4 genes was associated with differential response to demethylation therapy. A relatively low methylation percentage in one or both of these two genes was also associated with longer OS after decitabine based chemotherapy. We also suggest that global DNA and Oct-4/Sox2 methylation might impact on the pathogenesis of leukemia and play an important role in the initiation and progression. Moreover, dynamic analysis of 5-mC and Oct-4/Sox2 in peripheral blood nucleated cells of leukemia patients may provide clues to important molecular diagnostic and prognostic targets.
Effect of synthesis conditions on the particle size and morphology of YAG powder
Jie-Guang Song,Fang Wang,Ming-Han Xu,Yin-Yan Ju,Yang-Liang Li,Shi-Bin Li,Gang-Chang Ji 한양대학교 세라믹연구소 2012 Journal of Ceramic Processing Research Vol.13 No.2
Yttrium aluminum garnet (YAG) is currently a widely applied structural and functional material. To prepare highperformance YAG ceramics we prepared pure YAG powder, after calcining the precursor at 1100 ºC, by co-precipitation with ammonia as the precipitant and aluminum nitrate as well as yttrium nitrate as raw materials. The preparation conditions for the YAG precursor were a pH of 9, a titration rate of 10 ml/ minute and a reaction time of 60 minutes. The mean paricle size of the YAG powder was 11ìm. The mean particle size for the YAG powder increased with an increase in the pH and at a higher pH the YAG particles were more irregular in shape. The mean particle size and particle size distribution range of the YAG powder increased with an increase in the titration rate. For the YAG powder with a higher mean particle size, the particle size distribution range was wider but the size of most YAG particles was still small and the YAG particles were nearly spherical.
Preparation and properties of ZrB2-YAG-Al2O3 ceramics
Jie-Guang Song,Gang-Chang Ji,Shi-Bin Li,Yang-Liang Li,Da-Ming Du,Lian-Meng Zhang,Yin-Yan Ju 한양대학교 세라믹연구소 2009 Journal of Ceramic Processing Research Vol.10 No.4
ZrB2, YAG and Al2O3 are widely applied because of some excellent properties, but ZrB2 is easily oxidized in high-temperature air. To achieve better properties in ZrB2 ceramics, high-density ZrB2-YAG-Al2O3 ceramics were prepared. Below 1000℃, the shrinkage of ZrB2-YAG-Al2O3 ceramics is less than that of ZrB2-YAG ceramics. From 1000℃ to 1600℃, the second largest shrinkage occurs. Above 1600℃, the shrinkage of ZrB2-YAG-Al2O3 ceramics is more than that of ZrB2-YAG ceramics. The fracture toughness of sintered ceramics from coated raw materials is higher than that of sintered ceramics with mixed raw materials with the same phases and phase content, the fracture toughness of ZrB2-YAG-Al2O3 ceramics is higher than that of ZrB2-YAG ceramics with the same raw materials. The weight gain of all types of ceramics is increased with all increase in the oxidation temperature, the weight gain of ceramics is reduced with all increase in the YAG-Al2O3 content and Al2O3 proportion, especially above 1500℃. ZrB2, YAG and Al2O3 are widely applied because of some excellent properties, but ZrB2 is easily oxidized in high-temperature air. To achieve better properties in ZrB2 ceramics, high-density ZrB2-YAG-Al2O3 ceramics were prepared. Below 1000℃, the shrinkage of ZrB2-YAG-Al2O3 ceramics is less than that of ZrB2-YAG ceramics. From 1000℃ to 1600℃, the second largest shrinkage occurs. Above 1600℃, the shrinkage of ZrB2-YAG-Al2O3 ceramics is more than that of ZrB2-YAG ceramics. The fracture toughness of sintered ceramics from coated raw materials is higher than that of sintered ceramics with mixed raw materials with the same phases and phase content, the fracture toughness of ZrB2-YAG-Al2O3 ceramics is higher than that of ZrB2-YAG ceramics with the same raw materials. The weight gain of all types of ceramics is increased with all increase in the oxidation temperature, the weight gain of ceramics is reduced with all increase in the YAG-Al2O3 content and Al2O3 proportion, especially above 1500℃.
Effect of raw materials on the properties of ZrB2-YAG-Al2O3 multi-phase ceramics
Jie-Guang Song,Fang Wang,Da-Ming Du,Yin-Yan Ju,Shi-Bin Li,Gang-Chang Ji,Lian-Meng Zhang 한양대학교 세라믹연구소 2014 Journal of Ceramic Processing Research Vol.15 No.2
ZrB2 belongs to a class of ceramics defined ultra-high-temperature ceramics with extremely high melting temperatures, but ZrB2 ceramics is difficultly sintered and easily oxidized. To make ZrB2 ceramics possess the high relative density and the better oxidation resistance. The effects of raw materials on the properties of ZrB2 composite were investigated. YAG and Al2O3 help for the densification of ZrB2 ceramics. Fracture toughness of sintered ceramics with coated powder is higher than that of sintered ceramics with mixed powder. The mechanical property of ZrB2-YAG-Al2O3 materials is higher than that of ZrB2-YAG materials. Oxidation layer thickness of sintered ceramics with coated powder is thinner than that of sintered ceramics with mixed powder. These results show the sintered ZrB2 based multi-phase ceramics with coated microstructure help to increase the mechanical properties and oxidation resistance. The oxidation resistance of ZrB2-YAG-Al2O3 ceramics is better than that of ZrB2-YAG materials.
SNPs of Excision Repair Cross Complementing Group 5 and Gastric Cancer Risk in Chinese Populations
Yang, Wan-Guang,Zhang, Shan-Feng,Chen, Ju-Wu,Li, Li,Wang, Wan-Peng,Zhang, Xie-Fu Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12
We conducted a case-control study to determine the association between several potential SNPs of excision repair cross complementing group 5 (XPG) and gastric cancer susceptibility, and roles of XPG polymorphisms in combination with H.pylori infection in determining risk of gastric cancer. In our study, we collected 337 newly diagnosed gastric cancer cases and 347 health controls. Three SNPs of XPG, rs2296147T>C, rs2094258C>T and rs873601G>A, were genotyped using the Taqman real-time PCR method with a 7900 HT sequence detector system. H. pylori infection was diagnosed by ELISA. By multivariate logistic regression analysis, the rs2296147 CC genotype was associated with a decreased risk of gastric cancer (OR=0.52, 95% CI=0.27-0.97), and rs2094258 TT was associated with elevated risk (OR=2.13, 95% CI=1.22-3.35). Positive H.pylori individuals with rs2094258 TT genotypes demonstrated increased risk of gastric cancer (OR=2.13, 95% CI=1.22-3.35), while rs2296147 CC was associated with lower risk among patients with negative H.pylori (OR=0.45, 95%CI=0.22-0.89). Our findings suggested that XPG polymorphisms might contribute to risk of gastric cancer among Chinese populations, but the effect needs to be further validated by larger sample size studies.