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Avila-Peltroche, Jose,Won, Boo Yeon,Cho, Tae Oh The Korean Society of Phycology 2022 ALGAE Vol.37 No.2
Light-emitting-diodes (LEDs) are a lighting source useful for the precise evaluation of light quality effect on biological systems. Despite the importance of light spectra on the regeneration of land plant protoplasts ("naked cells"), this factor has not been tested yet on protoplasts from multicellular algae. This study reports on the effects of pure primary colors (red, blue, and green), dichromatic (red plus blue, RB, 1 : 2) and white LEDs on protoplast regeneration from male and female Undaria pinnatifida gametophytes. We also evaluated the effect of different light spectra on pigment composition (chlorophyll a, chlorophyll c, and fucoxanthine), and the light intensities under the best condition on the regeneration process. In the early stages, blue or RB LEDs increased the percentage of dividing female protoplasts, whereas red, blue, and RB LEDs enhanced that of dividing male protoplasts. In the later stages, RB LEDs showed a positive effect only on the percentage of multiple rhizoid-like protrusions (male gametophyte). They also increased the final area of both regenerated gametophytes. The LEDs did not affect pigment composition in female gametophytes. In male gametophytes, in contrast, they reduced chlorophyll c, while blue, RB, and green LEDs decreased fucoxanthin. Under RB LEDs, the optimal light intensity was 80 µmol photons m<sup>-2</sup> s<sup>-1</sup> for female gametophytes and 40 to 60 µmol photons m<sup>-2</sup> s<sup>-1</sup> for male gametophytes. Our results suggest that dichromatic LED illumination (red-blue) improves regeneration of U. pinnatifida gametophyte-isolated protoplasts. Thus, dichromatic LEDs might a suitable light source for enhancing protoplast regeneration in brown seaweeds.
A new record of Feldmannia chitonicola from Korea based on laboratory culture and molecular data
Jose Avila-Peltroche,Antony Otinga Oteng'o,정소영,원부연,조태오 한국환경생물학회 2019 환경생물 : 환경생물학회지 Vol.37 No.3
Feldmannia chitonicola is reported as a new record from Korea based on morphological studies in laboratory-cultured materials and molecular analyses. F. chitonicola is mainly characterized by a small size (1-2 mm), erect filaments mostly unbranched, plurilocular sporangia produced on both prostrate and laterally on the basal part of erect filaments, and 2 types (lanceolate and ovate) of sporangia. In our cultures, sporangia production was slower at 10°C than in 16°C and 20°C. Our molecular analyses of rbcL and cox1 genes supported its independence from other congeners reported for Korea. This is the first report of F. chitonicola for western Pacific.
Protoplast Production from Sphacelaria fusca (Sphacelariales, Phaeophyceae) Using Commercial Enzymes
Jose Avila-Peltroche,Boo Yeon Won 한국해양바이오학회 2020 한국해양바이오학회지 Vol.12 No.1
Sphacelaria is a filamentous brown algal genus that can be epibiotic on macroalgae, marine plants, and sea turtles. Its important role in benthic ecosystems, exposure to different stressors (e.g., grazing), and use as a model organism make Sphacelaria ideal for assessing physiological responses of organisms to environmental inputs. Single-cell RNA sequencing is a powerful new probe for understanding environmental responses of organisms at the molecular (transcriptome) level, capable of delineating gene regulation in different cell types. In the case of plants, this technique requires protoplasts (“naked” plant cells). The existing protoplast isolation protocols for Sphacelaria use non-commercial enzymes and are low-yielding. This study is the first to report the production of protoplasts from Sphacelaria fusca (Hudson) S.F. Gray, using a combination of commercial enzymes, chelation, and osmolarity treatment. A simple combination of commercial enzymes (cellulase Onozuka RS, alginate lyase, and driselase) with chelation pretreatment and an increased osmolarity (2512 mOsm/L H₂O) gave a protoplast yield of 15.08 ± 5.31 × 10⁴ protoplasts/g fresh weight, with all the Sphacelaria cell types represented. Driselase had no crucial effect on the protoplast isolation. However, the increased osmolarity had a highly significant and positive effect on the protoplast isolation, and chelation pretreatment was essential for optimal protoplast yield. The protocol represents a significant step forward for studies on Sphacelaria by efficiently generating protoplasts suitable for cellular studies, including single-cell RNA sequencing and expression profiling.
A new record of Feldmannia chitonicola from Korea based on laboratory culture and molecular data
Avila-Peltroche, Jose,Oteng'o, Antony Otinga,Jeong, So Young,Won, Boo Yeon,Cho, Tae Oh Korean Society of Environmental Biology 2019 환경생물 : 환경생물학회지 Vol.37 No.3
Feldmannia chitonicola is reported as a new record from Korea based on morphological studies in laboratory-cultured materials and molecular analyses. F. chitonicola is mainly characterized by a small size (1-2 mm), erect filaments mostly unbranched, plurilocular sporangia produced on both prostrate and laterally on the basal part of erect filaments, and 2 types (lanceolate and ovate) of sporangia. In our cultures, sporangia production was slower at 10℃ than in 16℃ and 20℃. Our molecular analyses of rbcL and cox1 genes supported its independence from other congeners reported for Korea. This is the first report of F. chitonicola for western Pacific.
Alveolar ridge augmentation with the perforated and nonperforated bone grafts
de Avila, Erica Dorigatti,Filho, Jose Scarso,de Oliveira Ramalho, Lizete Toledo,Real Gabrielli, Mario Francisco,Pereira Filho, Valfrido Antonio Korean Academy of Periodontology 2014 Journal of Periodontal & Implant Science Vol.44 No.1
Purpose: Autogenous bones are frequently used because of their lack of antigenicity, but good osteoconductive and osteoinductive properties. This study evaluated the biological behavior of perforated and nonperforated cortical block bone grafts. Methods: Ten nonsmoking patients who required treatment due to severe resorption of the alveolar process and subsequent implant installation were included in the study. The inclusion criteria was loss of one or more teeth; the presence of atrophy of the alveolar process with the indication of reconstruction procedures to allow rehabilitation with dental implants; and the absence of systemic disease, local infection, or inflammation. The patients were randomly divided into two groups based on whether they received a perforated (inner surface) or nonperforated graft. After a 6-month healing period, a biopsy was performed and osseointegrated implants were installed in the same procedure. Results: Fibrous connective tissue was evident at the interface in patients who received nonperforated grafts. However, full union between the graft and host bed was visible in those who had received a perforated graft. Conclusions: We found that cortical inner side perforations at donor sites increased the surface area and opened the medullary cavity. Our results indicate an increased rate of graft incorporation in patients who received such perforated grafts.
Antony Otinga Oteng’o,Jose Avila-Peltroche,정소영,원부연,조태오 한국환경생물학회 2019 환경생물 : 환경생물학회지 Vol.37 No.3
Acinetospora filamentosa and Microspongium stilophorae are reported as new records from South Korea based on morphological and molecular analyses. A. filamentosa is mainly characterized by having the sparsely branched erect filaments, the scattered meristematic zones, forming crampons, plurilocular sporangia on both prostrate filaments and lower part of erect filaments, and spherical to oval unilocular sporangia formed either sessile or with a pedicel. M. stilophorae is an epiphytic thalli mostly on Stilophora sp. It is characterized by prostrate filaments with irregular cells, short erect filaments with short ramuli, phaeophycean hairs, uniseriate plurilocular sporangia on the terminal part of erect filament. Our molecular analyses of rbcL and cox1 genes reveals that A. filamentosa and M. stilophorae are nested within the clades of Acinetospora and Microspongium, respectively.
Anti-Inflammatory Activity of Aqueous and Alkaline Extracts from Mushrooms (Agaricus blazei Murill)
Padilha, Marina M.,Avila, Ana A.L.,Sousa, Pergentino J.C.,Cardoso, Luis Gustavo V.,Perazzo, Fabio F.,Carvalho, Jose Carlos T. The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.2
The effects of aqueous and alkaline extracts from Agaricus blazei Murill, an edible mushroom used as folk medicine in Brazil, Japan, and China to treat several illnesses, were investigated on the basis of the inflammatory process induced by different agents. Oral administration of A. blazei extracts marginally inhibited the edema induced by nystatin. In contrast, when complete Freund's adjuvant was used as the inflammatory stimulus, both extracts were able to inhibit this process significantly (P < .05, analysis of variance followed by Tukey-Kramer multiple comparison post hoc test), although it inhibited the granulomatous tissue induction moderately. These extracts were able to decrease the ulcer wounds induced by stress. Also, administration of extracts inhibited neutrophil migration to the exudates present in the peritoneal cavity after carrageenin injection. Therefore, it is possible that A. blazei extracts can be useful in inflammatory diseases because of activation of the immune system and its cells induced by the presence of polysaccharides such as $\beta$-glucans.
Antony Otinga Oteng’o,Jose Avila-Peltroche,So Young Jeong,Boo Yeon Won,Tae Oh Cho 한국환경생물학회 2018 환경생물 : 환경생물학회지 Vol.36 No.3
Acinetospora asiatica and Botrytella reinboldii are reported as two new records from S. Korea based on morphological studies and molecular analyses. A. asiatica is mainly characterized by the presence of sparsely branched uniseriate filaments with diffused meristematic zones, the formation of crampons at right angles, and the formation of plurilocular sporangia on both prostrate and erect filaments. B. reinboldii is characterized by the presence of irregularly alternating branched uniseriate filaments attached by rhizoids, and single or clustered plurilocular sporangia with a single opening. Molecular analyses of rbcL gene revealed that A. asiatica and B. reinboldii are placed within each clade of Acinetospora and Botrytella, respectively.
Oteng'o, Antony Otinga,Avila-Peltroche, Jose,Jeong, So Young,Won, Boo Yeon,Cho, Tae Oh Korean Society of Environmental Biology 2018 환경생물 : 환경생물학회지 Vol.36 No.3
Acinetospora asiatica and Botrytella reinboldii are reported as two new records from S. Korea based on morphological studies and molecular analyses. A. asiatica is mainly characterized by the presence of sparsely branched uniseriate filaments with diffused meristematic zones, the formation of crampons at right angles, and the formation of plurilocular sporangia on both prostrate and erect filaments. B. reinboldii is characterized by the presence of irregularly alternating branched uniseriate filaments attached by rhizoids, and single or clustered plurilocular sporangia with a single opening. Molecular analyses of rbcL gene revealed that A. asiatica and B. reinboldii are placed within each clade of Acinetospora and Botrytella, respectively.