초임계 유체를 이용한 미세조류 바이오디젤의 직접 생산

유종훈,문성식 水原大學校 2014 論文集 Vol.28 No.-

In this study, we attempted to evaluate the feasibility of a supercritical methanol process for direct production of biodiesel from microalgae. The effects of various factors such as temperature, reaction time, water content and pretreatment on the efficiency of biodiesel conversion were investigated. The conversion efficiency increased with both temperature and extraction/conversion time. The supercritical methanol process produced a fatty acid methyl ester (FAME) yield of around 88% within 20 minutes. The optimal conditions for this process were found to be a microalgae/methanol ratio of around 1:9, a reaction temperature of about 270℃, and a reaction time of approximately 20 minutes. The effect of water content in microalgae samples on the FAME conversion was also evaluated. The supercritical methanol process was not affected by the water content up to 250 wt%. Our experiments demonstrated that the supercritical methanol process is more effective compared with conventional in situ transesterification processes.

김치가 노화촉진쥐 간의 유리기 생성 및 항산화효소 활성에 미치는 영향

김종현,권명자,이소영,류재두,문갑순,최홍식,송영옥 부산대학교 김치연구소 2002 김치의 과학과 기술 Vol.8 No.-

본 연구는 김치가 노화 억제에 미치는 영향을 살펴보기 위하여 AIN-76마우스 식이에 배추, 갓김치, 갓배추김치를 각 동결건조 김치를 5%씩 첨가하여 만든 후 김치식이를 노화촉진쥐인 SAMP8에게 1년간 섭취시키면서 간의 노화관련 특성인 유리기 농도와 항산화효소계의 효소활성 변화를 살펴 보았다. 노화촉진쥐 간의 총유리기, OH radical, H_2O_2 그리고 TBARS모두 가령에 따라 증가되었으며, 간에서 생성된 유리기 농도와 TBARS 농도는 대조군에 비해 김치섭취시 유의적으로 감소되었다(p<0.05). 그러나 생성된 유리기를 제거하기 위한 SOD활성은 오히려 김치섭취군에서 유의적으로 증가되었으며(p<0.05), catalase와 GSH-px, GSH/GSSG의 비율도 김치섭취군에서 유의적으로 증가하였다(p<0.05). 따라서 김치섭취는 노화에 따라 발생되는 간의 유리기 및 TBARS 생성은 억제시키면서, 이를 제거하는 항산화계 효소는 활성화시켜 노화를 억제한 것으로 생각된다. 김치종류 중에서는 배추김치보다 배추갓김치와 갓김치가 유리기 생성 억제 및 항산화 효소 활성을 더욱 증진시킨 것으로 관찰되어 이는 첨가된 갓의 항산화물질 때문인 것으로 생각된다. This study was performed to investigate the effect of kimchi intake on antiaging characteristics in the brain of senescence-accelerated mouse (SAM) in terms of free radical production and anti-oxidative enzymes. Two hundreds twenty SAM (20 mice) were divided into four groups and fed kimchi diet for 12 months. Experimental groups were kimchi free AIN-76 diet (control) group, Korean cabbage kimchi diet (KCK)group, 30% mustard leaf added Korean cabbage kimchi diet (MKCK) group, and mustard leaf kimchi diet (MLK) group. Concentrations of total free radical, OH radical and H_2O_2 of control group increased up to 123%, 262% and 174% of initial value (p<0.05) after one year. Increase in free radical production in kimchi groups due to aging was decreased by kimchi feeding. Among kimchi groups. MKCK and MLK groups showed greater inhibiting effect against free radical production than KCK. The concentration of TBARS in the bruin of control group also significantly increased up to 362% of initial value as aged (p<0.05) and production of TBARS in kimchi groups were decreased. When the activities of Cu, Zn-SOD, Mn-SOD, GSH-px and catalase of kimchi groups were compared to those of control at the same experimental period, anti-oxidative enzyme activities of kimchi groups were lower than those of control (p<0.05). But GSH/GSSG in kimchi groups were higher compared to control. In conclusion, decrease in free radical production and increase in anti-oxidative enzyme activities were observed from kimchi groups suggesting that kimchi might have important role on retarding aging. Among kimchi variety tested in this experiment, MKCK and MLK seem to have greater effect on inhibiting free radical production and increasing anti-oxidative enzyme activities than KCK. Further study is needed to find out the mechanism of kimchi on retarding aging.

김치가 노화촉진쥐 뇌의 유리기 생성 및 항산화효소 활성에 미치는 영향

김종현,류재두,이한기,박정희,문갑순,최홍식,송영옥 부산대학교 김치연구소 2002 김치의 과학과 기술 Vol.8 No.-

본 연구는 김치가 노화와 직접적인 관련이 있는 유리기 생성 및 항산화 효소활성에 미치는 영향을 살펴보기 위하여 배추김치, 배추갓김치 그리고 갓김치를 식이중량의 5% 첨가하여 실험식이를 제조하여 이를 노화촉진쥐인 SAMP8에게 1년간 섭취시켰다. 총유리기, OH radical, H_2O_2농도 그리고 TBARS 농도 모두 가령에 따라 증가하였으며, 김치섭취시 유의적으로 감소되었다(p<0.05), 김치군에 있어서 생성된 유리기를 제거하기 위해 항산화계 효소인 SOD, catalase, GSH-px 그리고 GSH/GSSG는 모든 김치군에서 유의적으로 증가 하였다(P<0.05). 따라서 김치섭취는 먼저 가령에 의해 생성되는 유리기의 생성은 억제시키면서, 둘째 이를 제거하기 위한 항산화계 효소 활성은 더욱 활성화시키므로써 항노화 기능을 발휘한다고 생각된다. 김치종류 중에서는 배추갓김치와 갓김치가 배추김치보다 유리기 생성을 억제하고, 항산화계 활성을 증진시킨 것으로 보아 이들의 항노화 효과가 클것으로 생각된다. This study was performed to investigate the effect of kimchi intake on antiaging characteristics in the brain of senescence-accelerated mouse (SAM) in terms of free radical production and anti-oxidative enzymes. Two hundreds twenty SAM (20 mice) were divided into four groups and fed kimchi diet for 12 months. Experimental groups were kimchi free AIN-76 diet (control) group, Korean cabbage kimchi diet (KCK)group, 30% mustard leaf added Korean cabbage kimchi diet (MKCK) group, and mustard leaf kimchi diet (MLK) group. Concentrations of total free radical, OH radical and H_2O_2 of control group increased up to 123%, 262% and 174% of initial value (p<0.05) after one year. Increase in free radical production in kimchi groups due to aging was decreased by kimchi feeding. Among kimchi groups. MKCK and MLK groups showed greater inhibiting effect against free radical production than KCK. The concentration of TBARS in the bruin of control group also significantly increased up to 362% of initial value as aged (p<0.05) and production of TBARS in kimchi groups were decreased. When the activities of Cu, Zn-SOD, Mn-SOD, GSH-px and catalase of kimchi groups were compared to those of control at the same experimental period, anti-oxidative enzyme activities of kimchi groups were lower than those of control (p<0.05). But GSH/GSSG in kimchi groups were higher compared to control. In conclusion, decrease in free radical production and increase in anti-oxidative enzyme activities were observed from kimchi groups suggesting that kimchi might have important role on retarding aging. Among kimchi variety tested in this experiment, MKCK and MLK seem to have greater effect on inhibiting free radical production and increasing anti-oxidative enzyme activities than KCK.

디젤승용 차량에서 배출되는 가스상 물질과 극미세 입자상 물질을 차량시험모드에 따른 실험적 분석

김세준,정민원,김병준,유종식,차경옥 明知大學校 産業技術硏究所 2007 産業技術硏究所論文集 Vol.26 No.-

Recently, the nano-PM's number concentration emitted by diesel internal combustion engine has drawn attentions because this particulate matters are suspected being hazardous of human health. In this study, The nano-PM mass and size of diesel passenger vehicles were measured on chassis dynamometer test bench. The particulate matters (PM) emissions of these vehicles were investigated for number concentration too. A condensation particle counter (CPC) system was applied to measure the particle number and size concentration of diesel exhaust particles at the end of dilution tunnel along the NEDC(ECE1 5+EUDC) and CVS-75 vehicle test mode. As the research result, the characteristic of vehicle test mode on the diesel nano-particle number and size distribution was investigated in this study.

바이오 디젤유를 차량 적용 시 입자 배출가스 특성에 관한 연구

김세준,정민원,김병준,유종식,차경옥 明知大學校 産業技術硏究所 2007 産業技術硏究所論文集 Vol.26 No.-

In this study, diesel passenger vehicle were measured on. chassis dynamometer test bench. this base engine was investigated by number measurement. so the particulate matter and gaseous emission were measured each test vehicle, fuel, mode condition on chassis dynamometer test bench that using the apparatus recommended by PMP(Particle Measurement Program) A condensation particle counter (CPC) system was applied to measure the particle number and size concentration of diesel exhaust particles at the end of dilution tunnel along the NEDC vehicle test mode. The effects of Bio diesel fuel on the Diesel nano particle number and size distribution was investigated in this study.

홈이 있는 내부 원통에서 홈의 깊이가 Taylor Vortex 형성에 미치는 영향

승삼선,민종식,유신오 三陟大學校 産業科學技術硏究所 2003 産業科學技術硏究論文集 Vol.8 No.2

We have used the technique of visualization to study influences on Taylor vortex flow formation in a fluid contained between concentric cylinders with grooved rotating inner cylinders. Where the grooved rotating inner cylinders mean having the different radius ratio compared with another straight part of inner cylinder. And they have different groove's depth in each grooved inner cylinders. Major parameters in these experiments are radius ratio by the shapes of inner cylinder. and angular velocity of inner rotating cylinder. So we found that results of this experimental study as follows. 1) A radius ratio has an effect on width of bands in rotating inner cylinder. In grooved sections. band width is wider than other straight section of inner cylinder. Especially in case of 1G9 and 1G12, groove's depth is related to unifying one wide band in grooved section. 2) In higher angular velocity. strong source flow occurred toward center of grooved section in case of 1G9 and 1G12. So it is guessed that groove's depth plays a important role in the process to promote turbulent Taylor vortex.

Status of Chemical Composition to Rainwater at an Agricultural Watershed

Jong-Soo Ryu,Jin-Ho Kim,Jong-Sik Lee,Won-Il Kim,Goo-Bok Jung 한국토양비료학회 2005 한국토양비료학회 학술발표회 초록집 Vol.2005 No.9

Experimental investigation of Mg isotope fractionation during mineral dissolution and clay formation

Jong-Sik Ryu,N. Vigier,A. Decarreai,Sin-Woo Lee,Kwang-Sik Lee,Hyoung-Suk Song,S. Petit 대한지질학회 2015 대한지질학회 학술대회 Vol.2015 No.10

A Case Study to Estimate the Greenhouse-Gas Mitigation Potential on Conventional Rice Production System

Jong-Hee Ryu,Jong-Sik Lee,Kye-Hoon Kim,Gun-Yeob Kim,Eun-Jung Choi 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.6

2011~2012년 2년간 전북 군산과 익산 지역의 관행농 벼를 재배하는 농가를 대상으로 온실가스 배출량 산정을 위한 인벤토리 목록을 구축하였다. 2년 누적 평균 데이터를 사용하여 전과정평가를 수행하고, 탄소성적 산출 및 온실가스배출의 주요인을 분석하였다. 분석된 온실가스 배출 주요인자들을 대상으로 민감도 분석을 수행하여 온실가스 잠재량을 산정하고, 대상지역 농가들이 적용할 수 있는 온실가스저감 영농법을 제안하고자 하였다. 관행농 쌀 생산농가를 대상으로 전과정 목록분석을 수행한 결과 탄소성적은 쌀 1 kg 생산을 기준으로 2.21 kg CO₂-eq. kg<SUP>-1</SUP>가 발생되었다. 온실가스 중 CO₂ 배출량이 가장 많았으나, 지구온난화 지수를 곱하여 이산화탄소 등가 (CO₂-eq.)로 환산하면 벼 생산체계의 탄소성적에서 메탄발생 기여도가 가장 컸다. 전체 CO₂ 배출량 중 복비생산 공정에서 37%가 발생하였고, 단비생산으로 10%, 벼 재배과정 중 40%가 발생하였다. 벼 재배 중 CO₂ 발생원은 농기계의 화석연료 사용에 의한 불완전 연소이다. CH₄는 대부분 벼 재배 중에 발생되었으며, 벼논의 메탄 발생 요인은 혐기조건의 담수논이다. N₂O은 대부분 벼 재배과정에서 배출되었고, 벼 재배 중 N₂O의 발생요인은 복비, 요소 비료, 퇴비 등의 비료시용이었다. 에너지 사용량 변화에 따른 민감도 분석결과 에너지원 중 경유의 민감도가 가장 높았고, 경유사용량을 10% 줄였을 때 약 2.5%의 CO₂ 감축 잠재량이 산정되었다. 복비 시용량을 10% 줄였을 때 CO₂는 약 1%, N₂O는 약 1.8%의 감축잠재량이 산정되었다. 퇴비시용을 10% 줄이면 약 1.5%의 메탄발생이 감소하고, 아산화질소는 약 1% 감소효과가 나타났다. 물떼기 일수가 10일 증가하면 메탄발생량이 약 4.5% 감소되었다. 투입량의 변화에 따른 온실가스 감소 효과가 가장 큰 요인은 벼논 물떼기 일수의 증가 및 경운과 수확시 사용하는 농기계용 경유사용량 감소였다. 그에 따라 중간낙수 및 무경운 등이 탄소배출 저감 영농법으로 제시되었다. To estimate greenhouse gas (GHG) emission, we established inventory of conventional rice cultivation from farmers in Gunsan and Iksan, Jeonbuk province in 2011∼2012. This study was to calculate carbon footprint and to analyse the major factor of GHGs. We carried out a sensitivity analysis using the analyzed main factors of GHGs and estimated the mitigation potential of GHGs. Also we tried to suggest agricultural methods to reduce GHGs that farmers of this case study can apply. Carbon footprint of rice production unit of 1 kg was 2.21 kg CO₂ .-eq. kg<SUP>-1</SUP>. Although amount of CO₂ emissions is largest among GHGs, methane had the highest contribution of carbon footprint on rice production system after methane was converted to carbon dioxide equivalent (CO₂ -eq.) multiplied by the global warming potential (GWP). Source of CO₂ in the cultivation of rice farming is incomplete combustion of fossil fuels used by agricultural machinery. Most of the CH₄ emitted during rice cultivation and major factor of CH₄ emission is flooded paddy field in anaerobic condition. Most of the N₂ O emitted from rice cultivation process and major sources of N₂ O emission is application of fertilizer such as compound fertilizer, urea, orgainc fertilizer, etc. As a result of sensitivity analysis due to the variation in energy consumption, diesel had the highest sensitivity among the energies inputs. If diesel consumption is reduced by 10%, it could be estimated that CO₂ potential reduction is about 2.5%. When application rate of compound fertilizer reduces by 10%, the potential reduction is calculated to be approximately 1% for CO₂ and approximately 1.8% for N₂ O. When drainage duration is decreased until 10 days, methane emissions is reduced by approximately 4.5%. That is to say drainage days, tillage, and reducing diesel consumption were the main sources having the largest effect of GHG reduction due to changing amount of inputs. Accordingly, proposed methods to decrease GHG emissions were no-tillage, midsummer drainage, etc.

Isotopic and elemental hydrogeochemistry of the Han River, South Korea

Jong-Sik Ryu,Kwang-Sik Lee,Ho-Wan Chang 대한지질학회 2003 대한지질학회 학술대회 Vol.2003 No.