[PB-0062] Isolation of soybean ARGONAUTE protein involved in seed coat color pigmentation

Jinwon Lee(Jinwon Lee),Gyu Tae Park(Gyu Tae Park),Hyun Jo(Hyun Jo),Jeongyun Ko(Jeongyun Ko),Junbeom Park(Junbeom Park),Jeong-Dong Lee(Jeong-Dong Lee),Jong Tae Song(Jong Tae Song) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

[PB-0064] Isolation of Ab-rg rich saponin mutants from EMS-induced population in soybean

Junbeom Park(Junbeom Park),Jagadeesh Sundaramoorthy(Jagadeesh Sundaramoorthy ),Jinwon Lee(Jinwon Lee),Jeong-Dong Lee(Jeong-Dong Lee),Hak Soo Seo(Hak Soo Seo),Jong Tae Song(Jong Tae Song) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Development of a Two-Dimensional Nano-Displacement Measuring System Utilizing a 2D Combined Optical and X-ray Interferometer

Jinwon Park,Cheon Il Eom,Jaegun Jo,Jaewan Kim,Sangho Byun,Taebong Eom 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.48 No.I

A two-dimensional nano-displacement measuring system utilizing a combined optical and X-ray interferometer (COXI) was developed. The system consists of optical interferometers for twodimensional displacements and an X-ray interferometer. The X-ray interferometer was used to calibrate the nonlinearity of the optical interferometers. The X-ray interferometer can subdivide an optical interference signal with 0.2 nm linear scales. The measured nonlinearity of the heterodyne optical interferometer was less than 2 nm. The calibrated optical interferometers were used to measure two-dimensional nano-scale displacements, and the accuracy of the optical interferometers was enhanced to enable sub-nanometer measurements after the compensation.愧

inner side

Jinwon Park(박진원) 한국디자인학회 2022 한국디자인학회 국제초대전도록 Vol.2022 No.9

Slowdown of spring green-up advancements in boreal forests

Park, Hoonyoung,Jeong, Su-Jong,Ho, Chang-Hoi,Park, Chang-Eui,Kim, Jinwon Elsevier 2018 Remote sensing of environment Vol.217 No.-

<P><B>Abstract</B></P> <P>There is a consensus that the spring phenology of deciduous forests is advancing in response to global warming. Since the late 1990s, however, this tendency of spring phenology advancement has been weakened in over 60% of boreal forests, particularly in Siberia (−0.58 day yr<SUP>−1</SUP> for 1982–1997 vs. −0.17 day yr<SUP>−1</SUP> for 1982–2013) and northwestern North America (NWNA; −0.42 day yr<SUP>−1</SUP> for 1982–1997 vs. 0.07 day yr<SUP>−1</SUP> for 1982–2013). This study investigated the major factor in the weakening trends in the advancement of the start of the growing season (SOS) based on the satellite-observed normalized difference vegetation index (NDVI) in two regions by quantifying the effects of four climatic fields—winter duration (WD; the number of freezing days), pre-season temperature (PT; accumulated temperature from late winter to early spring), green-up temperature (GT; accumulated temperature around the green-up date), and pre-season precipitation (PR; accumulated precipitation before the green-up date)—on changes in the spring green-up trend. The GT explained the majority of the slowdown in the SOS trends in recent decades. In Siberia, the GT increases contributed to the advancement of the SOS during the 1980s and 1990s; however, the GT increase reduced to less than half of these periods resulting in a slowdown of the SOS advancing trend since the early 2000s. In NWNA, GT increases and WD shortening drove the SOS advancement until the late 1990s; however, both effects have been diminished to near zero to result in no further SOS advancements. This study demonstrates that the recent slowdown of the SOS advancing trends over Siberia and NWNA was largely attributed to the weakening of the warming trends, rather than the sensitivity changes of spring phenology to climate variables. This study suggests that the natural climate variability strongly affects the decadal variations in the boreal forest spring phenology.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Satellite data reveals a shift in large-scale spring phenology trends. </LI> <LI> The advancing of spring phenology weakened in boreal forests after the late 1990s. </LI> <LI> We assessed the influence of climate variables on the slowdown of phenology trends. </LI> <LI> This slowdown is attributed to weakened warming rather than changes in sensitivity. </LI> </UL> </P>

Metabolic profiling of Klebsiella oxytoca: evaluation of methods for extraction of intracellular metabolites using UPLC/Q-TOF-MS.

Park, Changhun,Yun, Seokhun,Lee, Sang Yup,Park, Kyungmoon,Lee, Jinwon Humana Press 2012 Applied biochemistry and biotechnology Vol.167 No.3

<P>The global pool of intracellular metabolites is a reflection of all the metabolic functions of an organism. In the absence of in situ methods capable of directly measuring metabolite pools, intracellular metabolite measurements need to be performed after an extraction procedure. In this study, we evaluated the optimization of technologies for generation of a global metabolomics profile for intracellular metabolites in Klebsiella oxytoca. Intracellular metabolites of K. oxytoca were extracted at the early stationary phase using six different common extraction procedures, including cold methanol, boiling ethanol, methanol/chloroform combinations, hot water, potassium hydroxide, and perchloric acid. The metabolites were subsequently collected for further analysis, and intracellular metabolite concentration profiles were generated using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry. During analysis, the stability of metabolites extracted using cold methanol was clearly higher than that obtained by other extraction methods. For the majority of metabolites, extracts generated in this manner exhibited the greatest recovery, with high reproducibility. Therefore, the use of cold ethanol was the best extraction method for attaining a metabolic profile. However, in another parallel extraction method, perchloric acid may also be required to maximize the range of metabolites recovered, particularly to extract glucose 1-phosphate and NADPH.</P>

Performance Feedback and Risk Taking

Park Jinwon,Lee Kyungmook 한국경영학회 2018 한국경영학회 통합학술발표논문집 Vol.2018 No.8

We contribute to the performance feedback model by developing a theoretical framework that predicts the moderating effects of evaluation deadline proximity, remaining contract duration of CEO, and audience pressure between performance feedback and risk taking behaviors. We contend that in a negative attainment discrepancy context, deadline-proximity adjusted performance-aspiration gaps will increase the risk taking propensity of CEOs and remaining contract duration of CEOs and audience pressure will intensify the effects of deadline-proximity adjusted performance-aspiration gaps on risk taking behaviors. The analysis of the Major League Baseball games from 2007 to 2016 yields the following results. First of all, deadline-proximity adjusted performance-aspiration gaps showed predicted positive effects on risk taking behaviors. Second, the remaining contract duration of CEOs intensified the positive effect of the deadline-proximity adjusted performance-social aspiration gap on risk taking behaviors. Contrary to our predictions, however, the positive effect of the deadline-proximity adjusted performance-historical aspiration gap on risk taking behaviors decreased by audience pressure.

Targeted next-generation sequencing of well-differentiated rectal, gastric, and appendiceal neuroendocrine tumors to identify potential targets

Park, Ha Young,Kwon, Mi Jung,Kang, Ho Suk,Kim, Yun Joong,Kim, Nan Young,Kim, Min Jeong,Min, Kyueng-Whan,Choi, Kyung Chan,Nam, Eun Sook,Cho, Seong Jin,Park, Hye-Rim,Min, Soo Kee,Seo, Jinwon,Choe, Ji-Yo Elsevier 2019 Human pathology Vol.87 No.-

<P><B>Summary</B></P> <P>Rectal neuroendocrine tumors (NETs) are the most common gastrointestinal (GI) NETs with an uncertain malignant potential despite their small size. There are limited data about driver mutations in rectal NETs, which may explain the tumors' unexpected behavior or common histologic morphology with other GI-NETs. Here, we investigated the clinically and pathologically relevant mutations of rectal and nonrectal NETs and compared the frequency and clinical significance of detected mutations between them. We sequenced 84 primary GI-NETs (69 rectal, 7 gastric, 5 appendiceal, and 3 sigmoid colon NETs) and 3 metastatic GI-NETs using targeted next-generation sequencing. Twenty-one rectal NETs (30.4%) showed at least 1 mutation in 24 cancer-related genes; the most common mutations were <I>TP53</I> (10.1%) and <I>FBXW7</I> (7.2%), of which 73% were pathogenic/likely pathogenic mutations. <I>TP53</I> (p.R337C and p.R213*), <I>PTEN</I> (p.W111*, p.Q214*), <I>CDKN2A</I> (p.W110*), <I>FBXW7</I> (p.R465H), and <I>AKT1</I> (p.R23Q) were repetitive mutations found exclusively in rectal NETs, whereas <I>SMAD4</I> (p.R361C) and <I>STK11</I> (p.D176N) were repetitive mutations found only in gastric NETs. <I>PTEN</I> (p.G129K), <I>EGFR</I> (p.E709K), and <I>KIT</I> (p.V555I) were shared mutations between rectal and appendiceal NETs, whereas <I>SMAD4</I> (p.R361C), <I>ALK</I> (p.G1202R), <I>VHL</I> (p.Q132*), and <I>IDH1</I> (p.R132H) were concurrently detected between rectal and gastric NETs. GI-NETs with higher histologic grades, lymphovascular invasion, or recurrence tended to have higher numbers of mutation variants than other tumors; however, there was no significant difference. In conclusion, rectal NETs commonly carried pathogenic/likely pathogenic mutations. Because most mutations were identified in nonhotspot positions, next-generation sequencing is useful in identifying potential drug targets in rectal NETs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>TP53</I>, <I>PTEN</I>, and <I>SMAD4</I> are commonly mutated in gastrointestinal NETs. </LI> <LI> Seventy-five percent of mutations in rectal NETs are pathogenic/likely pathogenic mutations. </LI> <LI> Eighty-seven percent of genes are mutated in nonhotspot positions. </LI> </UL> </P>

High MET copy number and MET overexpression: poor outcome in non-small cell lung cancer patients.

Park, Sanghui,Choi, Yoon-La,Sung, Chang Ok,An, Jungsuk,Seo, Jinwon,Ahn, Myung-Ju,Ahn, Jin Seok,Park, Keunchil,Shin, Young Kee,Erkin, Ozgur Cem,Song, Kyung,Kim, Jhingook,Shim, Young Mog,Han, Joungho Gutenberg 2012 Histology and histopathology Vol.27 No.2

<P>The aim of this study was to evaluate the prevalence and prognostic role of increased gene copy number and protein expression of MET and EGFR in non-small cell lung cancer (NSCLC) patients. Samples were collected from 380 patients with surgically resected NSCLC, and fluorescence in situ hybridisation (FISH) and immunohistochemistry (IHC) were performed. EGFR amplification and high polysomy (EGFR FISH-positive) were observed in 9.7% and 17.4% of the patients, respectively. EGFR was overexpressed (EGFR IHC-positive) in 19.2% of the patients. Neither EGFR FISH-positive nor EGFR IHC-positive status affected survival after resection. Increased MET copy number (MET FISH-positive by University of Colorado Cancer Center criteria) was observed in 11.1% of the patients (high polysomy, 8.7%; gene amplification, 2.4%). According to the Cappuzzo system, 7.1% of the patients were MET FISH-positive. MET FISH positivity was a negative prognostic factor, especially in patients with adenocarcinoma histology (p=0.040), female gender (p=0.010), old age (p=0.084), and EGFR FISH negativity (p=0.020) at the univariate level but not at the multivariate level. MET was overexpressed (MET IHC-positive) in 13.7% of the patients and associated with shorter overall and disease-free survival (p=0.010 and p=0.056, respectively). Multivariate analysis revealed that MET IHC-positive patients had a significantly increased risk of death (hazard ratio, 1.618; 95% confidence interval, 1.066-2.456; p=0.024). Increased MET copy number and MET overexpression are negative prognostic factors for surgically resected NSCLCs.</P>

Bioactive Lipids and Their Derivatives in Biomedical Applications

( Jinwon Park ),( Jaehyun Choi ),( Dae-duk Kim ),( Seunghee Lee ),( Bongjin Lee ),( Yunhee Lee ),( Sanghee Kim ),( Sungwon Kwon ),( Minsoo Noh ),( Mi-ock Lee ),( Quoc-viet Le ),( Yu-kyoung Oh ) 한국응용약물학회 2021 Biomolecules & Therapeutics(구 응용약물학회지) Vol.29 No.5

Lipids, which along with carbohydrates and proteins are among the most important nutrients for the living organism, have a variety of biological functions that can be applied widely in biomedicine. A fatty acid, the most fundamental biological lipid, may be classified by length of its aliphatic chain, and the short-, medium-, and long-chain fatty acids and each have distinct biological activities with therapeutic relevance. For example, short-chain fatty acids have immune regulatory activities and could be useful against autoimmune disease; medium-chain fatty acids generate ketogenic metabolites and may be used to control seizure; and some metabolites oxidized from long-chain fatty acids could be used to treat metabolic disorders. Glycerolipids play important roles in pathological environments, such as those of cancers or metabolic disorders, and thus are regarded as a potential therapeutic target. Phospholipids represent the main building unit of the plasma membrane of cells, and play key roles in cellular signaling. Due to their physical properties, glycerophospholipids are frequently used as pharmaceutical ingredients, in addition to being potential novel drug targets for treating disease. Sphingolipids, which comprise another component of the plasma membrane, have their own distinct biological functions and have been investigated in nanotechnological applications such as drug delivery systems. Saccharolipids, which are derived from bacteria, have endotoxin effects that stimulate the immune system. Chemically modified saccharolipids might be useful for cancer immunotherapy or as vaccine adjuvants. This review will address the important biological function of several key lipids and offer critical insights into their potential therapeutic applications.