A cisplatin-incorporated liposome that targets the epidermal growth factor receptor enhances radiotherapeutic efficacy without nephrotoxicity

JUNG, JOOHEE,JEONG, SEONG-YUN,PARK, SEOK SOON,SHIN, SEOL HWA,JU, EUN JIN,CHOI, JINHYANG,PARK, JAESOOK,LEE, JAE HEE,KIM, INKI,SUH, YOUNG-AH,HWANG, JUNG JIN,KURODA, SHUN’ICHI,LEE, JUNG SHIN,SONG, SI YEO Spandidos Publications 2015 International journal of oncology Vol.46 No.3

<P>Radiotherapy (RT) is one of the major modalities for non???small cell lung cancer (NSCLC), but its efficacy is often compromised by cellular resistance caused by various mechanisms including the overexpression of epidermal growth factor receptor (EGFR). Although cis???diamminedichloroplatinum(???) (cisplatin, CDDP) has been well characterized as an effective radiosensitizer, its clinical application is limited by its severe nephrotoxic effects. In our current study, we developed a CDDP???incorporated liposome (LP) conjugated with EGFR antibodies (EGFR:LP???CDDP) and evaluated its potential to radiosensitize EGFR???overexpressing cells without exerting nephrotoxic effects. EGFR:LP???CDDP showed higher cytotoxicity than non???targeting liposomal CDDP (LP???CDDP) in the cells expressing EGFR in vitro. In an A549 cell???derived xenograft tumor mouse model, increased delays in tumor growth were observed in the mice treated with a combination of EGFR:LP???CDDP and radiation. Notably, the EGFR:LP???CDDP???treated animals showed no differences in body weight loss, survival rates of nephrotoxicity compared with untreated control mice. In contrast, the use of CDDP caused lower body weights and poorer survival outcomes accompanied by a significant level of nephrotoxicity [e.g., decreased kidney weight, increased blood urea nitrogen (BUN) and creatinine, and pathological change]. These findings suggest the feasibility of using EGFR:LP???CDDP to radiosensitize cells in a targeted manner without inducing nephrotoxic effects. This compound may therefore have clinical potential as part of a tailored chemoradiotherapy strategy.</P>

Nanoparticulated docetaxel exerts enhanced anticancer efficacy and overcomes existing limitations of traditional drugs

Choi, Jinhyang,Ko, Eunjung,Chung, Hye-Kyung,Lee, Jae Hee,Ju, Eun Jin,Lim, Hyun Kyung,Park, Intae,Kim, Kab-Sig,Lee, Joo-Hwan,Son, Woo-Chan,Lee, Jung Shin,Jung, Joohee,Jeong, Seong-Yun,Song, Si Yeol,Cho Dove Medical Press 2015 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.10 No.-

<P>Nanoparticulation of insoluble drugs improves dissolution rate, resulting in increased bioavailability that leads to increased stability, better efficacy, and reduced toxicity of drugs. Docetaxel (DTX), under the trade name Taxotere™, is one of the representative anticancer chemotherapeutic agents of this era. However, this highly lipophilic and insoluble drug has many adverse effects. Our novel and widely applicable nanoparticulation using fat and supercritical fluid (NUFS™) technology enabled successful nanoscale particulation of DTX (Nufs-DTX). Nufs-DTX showed enhanced dissolution rate and increased aqueous stability in water. After confirming the preserved mechanism of action of DTX, which targets microtubules, we showed that Nufs-DTX exhibited similar effects in proliferation and clonogenic assays using A549 cells. Interestingly, we observed that Nufs-DTX had a greater in vivo tumor growth delay effect on an A549 xenograft model than Taxotere™, which was in agreement with the improved drug accumulation in tumors according to the biodistribution result, and was caused by the enhanced permeability and retention (EPR) effect. Although both Nufs-DTX and Taxotere™ showed negative results for our administration dose in the hematologic toxicity test, Nufs-DTX showed much less toxicity than Taxotere™ in edema, paralysis, and paw-withdrawal latency on a hot plate analysis that are regarded as indicators of fluid retention, peripheral neuropathy, and thermal threshold, respectively, for toxicological tests. In summary, compared with Taxotere™, Nufs-DTX, which was generated by our new platform technology using lipid, supercritical fluid, and carbon dioxide (CO<SUB>2</SUB>), maintained its biochemical properties as a cytotoxic agent and had better tumor targeting ability, better in vivo therapeutic effect, and less toxicity, thereby overcoming the current hurdles of traditional drugs.</P>

내부회계관리･운영조직 인력의 특성이 회계정보의 가치관련성에 미치는 영향

마희영(Ma HeeYoung),정진향(Jung JinHyang) 한국산업경영학회 2016 經營硏究 Vol.31 No.2

본 연구는 회계정보의 신뢰성을 제고하기 위해 도입된 내부회계관리제도가 도입 목적에 부합하는 지를, 내부회계관리･운영조직 인력의 특성(규모와 전문성)이 회계정보의 가치관련성에 미치는 영향을 실증 분석함으로써 살펴보고자 한다. 2004년부터 2012년까지 유가증권시장에 상장된 12월 결산, 비금융업을 분석하였고 표본 수 (firm-year)는 4,432개이다. 실증분석결과 첫째, 내부회계관리･운영조직 인력의 규모와 가치관련성 은 유의한 양(+)의 관계를 보여, 내부회계관리업무를 담당하는 인원의 수가 많을수록 가치관성은 증가하는 것으로 나타났다. 둘째, 내부회계관리･운영조직 중 회계부서의 인력규모와 가치관련성은 유의한 양(+)의 관계를 보였다. 또한, 내부회계관리업무를 담당하는 인력 중 공인회계사 자격증 보유자의 수와 가치관련성은 유의한 양(+)의 관계로 나타났다. 이러한 결과는 내부회계관리업무를 담당하는 인력이 회계에 대한 전문성이 있을 때 회계정보의 가치관련성이 증가하는 것으로 해석할 수 있다. 본 연구의 결과는 내부회계관리제도가 실효성 있게 운영되기 위해서는 내부회계관리업무를 담 당하는 인적 자원에 대한 투자가 중요함을 나타낸다. This study presents analysis on the effectiveness of internal accounting control to the reliability of accounting information. This study also aims to identify certain factors (i.e. company size and company profiling) which can influence the effectiveness of internal accounting control. A sample size of 4,432 numbers from selected stock market accounts (from December 2004 to December 2012) was used and analyzed for this study. Results show that the size of the company’s organization (number of personnel) is directly proportional to the increase in effectiveness of the system. In addition, more positive results were expected when the personnel in charge of the internal accounting controls are adept with accounting and finance e.g. certified public accountant. These results translate to the importance of a person’s expertise with respect to the quality of delivered work or system. Based from this study, it is recommended that investment in human resources with relevant skills and competencies should be given higher priority and value in order to have effective and efficient internal accounting controls system.

교양교육 체계 마련 및 적용 가능 연구

이수재 ( Soojae Lee ),정진향 ( Jinhyang Jung ),김지아 ( Jia Kim ),김정진 ( Jungjin Kim ) 한국고등직업교육학회 2023 한국고등직업교육학회논문집 Vol.21 No.1

대학의 중장기 발전계획과 졸업생의 요구에 따라 미래지향적 사회 비전을 반영한 Y대학교의 특성화 교양교육시스템 구축 및 적용 연구가 필요한 실정이다. 이에 따라 본 연구에서는 Y대학교 교양교육 프로그램의 역할을 재정립하고, 교양교육의 질을 체계적으로 높일 수 있는 기반을 마련하고자 하였다. In accordance with the university’s medium to long-term development plan and the implementation of the desired qualities of graduates, there is a demand for the establishment and applicable research of a specialized liberal arts education system at Y University that reflects a future-oriented social vision. In line with this, the goal is to redefine the role of Y University’s liberal arts education program and provide a foundation of resources to systematically enhance the quality of liberal arts education.

Novel peptides functionally targeting in vivo human lung cancer discovered by in vivo peptide displayed phage screening.

Lee, Kyoung Jin,Lee, Jae Hee,Chung, Hye Kyung,Choi, Jinhyang,Park, Jaesook,Park, Seok Soon,Ju, Eun Jin,Park, Jin,Shin, Seol Hwa,Park, Hye Ji,Ko, Eun Jung,Suh, Nayoung,Kim, InKi,Hwang, Jung Jin,Song, S Springer-Verlag ; Springer 2015 Amino acids Vol.47 No.2

<P>Discovery of the cancer-specific peptidic ligands have been emphasized for active targeting drug delivery system and non-invasive imaging. For the discovery of useful and applicable peptidic ligands, in vivo peptide-displayed phage screening has been performed in this study using a xenograft mouse model as a mimic microenvironment to tumor. To seek human lung cancer-specific peptides, M13 phage library displaying 2.9 ?? 10(9) random peptides was intravenously injected into mouse model bearing A549-derived xenograft tumor through the tail vein. Then the phages emerged from a course of four rounds of biopanning in the xenograft tumor tissue. Novel peptides were categorized into four groups according to a sequence-homology phylogenicity, and in vivo tumor-targeting capacity of these peptides was validated by whole body imaging with Cy5.5-labeled phages in various cancer types. The result revealed that novel peptides accumulated only in adenocarcinoma lung cancer cell-derived xenograft tissue. For further confirmation of the specific targeting ability, in vitro cell-binding assay and immunohistochemistry in vivo tumor tissue were performed with a selected peptide. The peptide was found to bind intensely to lung cancer cells both in vitro and in vivo, which was efficiently compromised with unlabeled phages in an in vitro competition assay. In conclusion, the peptides specifically targeting human lung cancer were discovered in this study, which is warranted to provide substantive feasibilities for drug delivery and imaging in terms of a novel targeted therapeutics and diagnostics.</P>

갑상선 수질암의 임상적 고찰

육종필,신정혜,황승욱,정진향,박호용,Jongpil Ryuk,Junghye Shin,Seunguk Hwang,Jinhyang Jung,and Hoyong Park 대한갑상선-내분비외과학회 2007 The Koreran journal of Endocrine Surgery Vol.7 No.1