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        Cloning and different expression of ATP synthase genes between propargite resistant and susceptible strains of Tetranychus cinnabarinus (Acarina: Tetranychidae)

        Shu-Jun Wei,Jing Ni,Kuanyu Zheng,Zhenguo Yang,Daoyan Xie,Aisi Da,Jianping Chai,Xiujun Jiang,Shaoxiang Li 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

        The carmine spider mite, Tetranychus cinnabarinus (Boisduval), is a serious phytophagous mite damaging importantcrops and can rapidly develop resistance to acaricides. Mitochondrial ATP synthase (F1F0 ATP synthase)is an important target site of acaricides. The role of ATP synthase in acaricide resistance remains unclear at themolecular level. In this study, twelve full-length cDNAs of ATP synthase genes were cloned and characterizedfrom T. cinnabarinus and their expression levels were determined for both progargite-resistant and susceptiblestrains. The effect of propargite exposure on gene expression was also evaluated. Analyses of gene expressionrevealed that TcATPsynU-2, TcATPsynF0-2 and TcATPsynF0-4 were significantly down-regulated in the progargite-resistant strain. TcATPsynF0-2 and TcATPsynF0-4 had a strong response to progargite exposure. Theresults suggest that lower levels of TcATPsynU-2, TcATPsynF0-2 and TcATPsynF0-4 expression might be related topropargite-resistance observed in the resistant T. cinnabarinus. This is the first attempt to identify specific ATPasegenes involved in propargite resistance in T. cinnabarinus.

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        The influence of the extent of lymph node metastasis on the prognosis for patients with intrahepatic cholangiocarcinoma

        Jianping Wang*,Man Shu*,Hong Peng,Shaoqiang Li,Dongming Li,Jingxian Shen,Ming Kuang,Ying Zhang,Zebin Chen 대한외과학회 2023 Annals of Surgical Treatment and Research(ASRT) Vol.104 No.5

        Purpose: Reports showed that some of intrahepatic cholangiocarcinoma (ICC) patients with lymph node metastasis (LNM) may also gain survival benefit undergone resection. However, the effect of the extent of LNM on prognosis and surgical indication is barely discussed. Methods: From September 1994 to November 2018, primary ICC patients undergone initial curable surgery were enrolled. Based on the extent of LNM, we divided these patients into 4 groups, including patients with no LNM (group N0), LNM to hepatoduodenal ligament or common hepatic artery (region A, group A), LNM to gastrohepatic lymph nodes for left liver ICC and periduodenal and peripancreatic lymph node for right liver ICC (region B, group B), or LNM beyond these regions (region C, group C). Multivariable Cox regression analysis was performed to identify the prognostic factors for recurrence- free survival (RFS) and overall survival (OS) in all groups. Results: A total of 133 patients were enrolled. There were 56, 21, 17, and 39 patients in groups N0, A, B, and C, respectively. There was significant difference between groups N0 and C in RFS (P < 0.001) and OS (P = 0.002). When we compared group N0 + A + B with group C, we also found that RFS (P < 0.001) and OS (P = 0.007) were significantly different. In multivariable analysis, the extent of LNM was an independent risk factor for RFS (P < 0.050). Conclusion: ICC patients with the LNM to regions A and B could still achieve good prognosis with resection. Surgery should be carefully considered when LNM to region C.

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        Comparison of pre-indwelling double-J stents versus ureteral catheters for artificial hydronephrosis in percutaneous nephrolithotomy: A retrospective cohort study

        Tingshuai Yan,Keli Quan,Cong Yan,Tong Yang,Yingqi Zhao,Jianping Shu,Gaofei He 대한비뇨의학회 2022 Investigative and Clinical Urology Vol.63 No.4

        Purpose: To compare the clinical efficacy and safety of pre-indwelling double-J stents versus ureteral catheters for artificial hydronephrosis in percutaneous nephrolithotomy (PCNL). Materials and Methods: We retrospectively analyzed the data of 1,258 patients who underwent PCNL for kidney stones from August 2017 to July 2020 in our hospital. Among them, 682 patients had double-J stents inserted (DJ group) and 576 patients had ureteral catheters (UC group). We analyzed baseline patient characteristics, perioperative outcomes, and complications in both groups. Results: The puncture success rate was 97.9% and 97.4% in the DJ and UC groups, respectively (p>0.05). The operation time was 74.5±37.8 minutes in the DJ group compared with 80.8±38.5 minutes in the UC group (p=0.004). The total stone-free rate in the DJ and UC groups was 80.5% and 78.7%, respectively (p>0.05). The incidence of perioperative complications was relatively low in both groups and showed no obvious differences. In the subgroup analysis, the operation time for patients with no obvious or mild hydronephrosis preoperatively was significantly shorter in the DJ group than in the UC group (p<0.05). However, there were no significant differences among patients who had moderate or severe hydronephrosis preoperatively. Conclusions: It is feasible, safe, and effective to create artificial hydronephrosis by insertion of pre-indwelling double-J stents in PCNL surgery. Furthermore, the operation time was significantly shorter in the DJ group than in the group with pre-indwelling ureteral catheters, especially in patients who had no obvious or mild hydronephrosis preoperatively.

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