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- 검색키워드
- 저자 : Jianlei Jia (검색결과 4 건)
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Jianlei Jia,Qian Chen,Lin-sheng Gui,Jipeng Jin,Yongyuan Li,Qiaohong Ru,Shengzhen Hou 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.7
Objective: The present study was to investigate the association of polymorphisms in exon-9 of the bone morphogenetic protein receptor-1B (BMPR-1B) gene (C864T) with litter size in 240 Dorset, 232 Mongolian, and 124 Small Tail Han ewes. Methods: Blood samples were collected from 596 ewes and genomic DNA was extracted using the phenol: chloroform extraction method. The 304-bp amplified polymerase chain reaction product was analyzed for polymorphism by single-strand conformation polymorphism method. The genotypic frequency and allele frequency of BMPR-1B gene exon-9 were computed after sequence alignment. The χ2 independence test was used to analyze the association of genotypic frequency and litter size traits with in each ewe breed, where the phenotype was directly treated as category. Results: The results indicated two different banding patterns AA and AB for this fragment, with the most frequent genotype and allele of AA and A. Calculated Chi-square test for BMPR-1B gene exon-9 was found to be more than that of p value at the 5% level of significance, indicating that the population under study was in Hardy-Weinberg equilibrium for all ewes. The χ2 independence test analyses indicated litter size differences between genotypes was not the same for each breed. The 304-bp nucleotide sequence was subjected to BLAST analysis, and the C864T mutation significantly affected litter size in singletons, twins and multiples. The heterozygosity in exon-9 of BMPR-1B gene could increase litter size for all the studied ewes. Conclusion: Consequently, it appears that the polymorphism BMPR-1B gene exon-9 detected in this study may have potential use in marker assisted selection for litter size in Dorset, Mongolian, and Small Tail Han ewes.
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A comprehensive evaluation method study for dam safety
Jia, Fan,Yang, Meng,Liu, Bingrui,Wang, Jianlei,Gao, Jiaorong,Su, Huaizhi,Zhao, Erfeng Techno-Press 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.63 No.5
According to the multi-index system of dam safety assessment and the standard of safety, a comprehensive evaluation model for dam safety based on a cloud model is established to determine the basic probability assignment of the Dempster-Shafer theory. The Dempster-Shafer theory is improved to solve the high conflict problems via fusion calculation. Compared with the traditional Dempster-Shafer theory, the application is more extensive and the result is more reasonable. The uncertainty model of dam safety multi-index comprehensive evaluation is applied according to the two theories above. The rationality and feasibility of the model are verified through application to the safety evaluation of a practical arch dam.
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A comprehensive evaluation method study for dam safety
Fan Jia,Meng Yang,Bingrui Liu,Jianlei Wang,Jiaorong Gao,Huaizhi Su,Erfeng Zhao 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.63 No.5
According to the multi-index system of dam safety assessment and the standard of safety, a comprehensive evaluation model for dam safety based on a cloud model is established to determine the basic probability assignment of the Dempster-Shafer theory. The Dempster-Shafer theory is improved to solve the high conflict problems via fusion calculation. Compared with the traditional Dempster-Shafer theory, the application is more extensive and the result is more reasonable. The uncertainty model of dam safety multi-index comprehensive evaluation is applied according to the two theories above. The rationality and feasibility of the model are verified through application to the safety evaluation of a practical arch dam.
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Lin-sheng Gui,Sayed Haidar Abbas Raza,Jianlei Jia 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.1
Objective: The oxidized low density lipoprotein receptor 1 (OLR1) gene plays an important role in the degradation of oxidized low-density lipoprotein and adipocyte proliferation in mammals. For this reason, we aimed at investigating the association of OLR1 gene polymorphisms with carcass quality traits in Chinese Qinchuan cattle. Methods: The single nucleotide polymorphism (SNP) was identified in the 3′ untranslated region of bovine OLR1 gene by DNA sequencing. In addition, the haplotype frequency and linkage disequilibrium estimates of three SNPs were evaluated in 520 individuals. Results: Results indicated that the studied three SNPs were within the range of moderate genetic diversity (0.25< polymorphism information content<0.5). Haplotype analysis of three SNPs showed that ten different haplotypes were identified, but only five haplotypes were listed as those with a frequency of <0.05 were excluded. The Hap3 (-G1T2C3-) had the highest haplotype frequency (42.10%). Linkage disequilibrium analysis showed that the three SNPs had a low linkage (r2<0.001). The T10588C and C10647T were significantly associated with backfat thickness and intramuscular fat content in Qinchuan cattle. Conclusion: Based on our results, we believe that the OLR1 gene could be a strong candidate gene for influencing carcass quality traits in Qinchuan cattle.
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