Prognostic Value of CD44 Variant exon 6 Expression in Non-Small Cell Lung Cancer: a Meta-analysis

Zhao, Shuang,He, Jin-Lan,Qiu, Zhi-Xin,Chen, Nian-Yong,Luo, Zhuang,Chen, Bo-Jiang,Li, Wei-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Background: CD44v6 (CD44 variant exon 6) is the chief CD44 variant isoform regulating tumor invasion, progression, and metastasis. The prognostic value of CD44v6 expression in non small cell lung cancer (NSCLC) has been evaluated in many studies, but the results have remained controversial. Thus, we performed a meta-analysis of currently available studies to investigate the prognostic value of CD44v6 expression in NSCLC patients and the relationship between the expression of CD44v6 and clinicopathological features. Materials and Methods: Two independent reviewers searched the relevant literature in Pubmed, Medline and Embase from 1946 to January 2014. Overall survival (OS) and various clinicopathological features were collected from included studies. This meta-analysis was accomplished using STATA 12.0 and Revman 5.2 software. Pooled hazard ratios (HRs) with 95% confidence intervals (95%CIs) were calculated to estimate the effects. Results: A total of 921 NSCLC patients from ten studies met the inclusion criteria. The results showed that CD44v6 high expression was a prognostic factor for poor survival (HR=1.91, 95%CI=1.12-3.26, p<0.05). With respect to clinicopathological features, CD44v6 high expression was related to histopathologic type (squamous cell carcinoma versus adenocarcinoma: OR=2.72, 95%CI=1.38-5.38, p=0.004), and lymph node metastasis (OR=3.02, 95%CI=1.93-4.72, p<0.00001). Conclusions: Our results suggested CD44v6 high expression as a poor prognostic factor for NSCLC, and CD44v6 expression is associated with lymph node metastasis and histopathologic type. Therefore, CD44v6 expression can be used as a novel prognostic marker in NSCLC cases.

Thermal Conductive Epoxy Enhanced by Nanodiamond-Coated Carbon Nanotubes

Bo Zhao,Guohua Jiang 대한금속·재료학회 2017 ELECTRONIC MATERIALS LETTERS Vol.13 No.6

Nanodiamond (ND) particles were coated on the surface of carbonnanotubes (CNTs) by chemical reactions. Reliable bonding wasformed by the combination of acyl chloride on NDs and amine groupon CNTs. ND coated CNTs (CNT-ND) were dispersed into epoxy tofabricate thermal conductive resins. The results show that the surfaceenergy of CNTs is decreased by the coated NDs, which is contributedto the excellent dispersion of CNT-NDs in the epoxy matrix. Theheat-transfer channels were built by the venous CNTs cooperatingwith the coated NDs, which not only plays an effective role of heatconduction for CNTs and NDs, but also avoids the electrical leakageby the protection of NDs surrounding outside of CNTs. Electrical andthermal conductance measurements demonstrate that the influence ofthe CNT-ND incorporation on the electrical conductance is minor,however, the thermal conductivity is improved significantly for theepoxy filled with CNT-ND.

Marine Collagen Peptides Prepared from Chum Salmon (Oncorhynchus keta) Skin Extend the Life Span and Inhibit Spontaneous Tumor Incidence in Sprague-Dawley Rats

Jiang Liang,Xin-Rong Pei,Nan Wang,Zhao-Feng Zhang,Jun-Bo Wang,Yong Li 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.4

To observe the effects of marine collagen peptides (MCPs) prepared from chum salmon (Oncorhynchus keta) skin on life span and spontaneous tumor incidence, Sprague-Dawley rats were fed diets supplemented with MCP at concentrations of 0%, 2.25%, 4.5%, and 9% (wt/wt) from the age of 4 weeks until natural death. There were 40 rats in each group (male:female ratio=1:1). The results showed that the MCP did not significantly influence body weight or food consumption of rats of either sex throughout the life span; it did dose-dependently inhibit the age-related decrease in the activities of antioxidant enzymes and the age-related increase in the levels of lipid peroxidation product in both sexes. MCP notably increased the mean life span, the life span of the last 30% of the survivors, and the maximal life span; it decreased overall spontaneous tumor incidence of both sexes with significance in the 4.5% and 9% MCP-treated male groups and 9% MCP-treated female group. Compared to the control group, the incidence of death from tumors was decreased in MCP groups in comparison with the control group of both sexes. Therefore, we concluded that MCPs dose-dependently increase life span and decrease spontaneous tumor incidence in Sprague-Dawley rats. Moreover, the antioxidative property of MCPs may be responsible for the increased life span and protection against tumor development.

Characterization of Two GAS1 Genes and Their Effects on Expression and Secretion of Heterologous Protein Xylanase B in Kluyveromyces lactis

( Zhao Lian ),( Ing Bo Jiang ),( Shuang Chi ),( Guo Hua Guan ),( Ying Li ),( Ji Lun Li ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.12

β-1,3-glucanosyltransferases play essential roles in cell wall biosynthesis in yeast. Kluyveromyces lactis has six putative β-1,3-glucanosyltransferase genes. KlGAS1-1 and KlGAS1-2 are homologs of Saccharomyces cerevisiae gene GAS1. RT-qPCR indicated the transcription level of KlGAS1-1 was significantly reduced while heterologous protein (thermostable xylanase B) secretion was enhanced during medium optimization. To evaluate if these two events were related, and to improve xylanase B secretion in K. lactis, we constructed KlGAS1-1 and KlGAS1-2 single deletion strains and double deletion strain, respectively. KlGAS1-1 gene deletion resulted in the highest xylanase B activity among the three mutants. Only the double deletion strain showed morphology similar to that of the GAS1 deletion mutant in S. cerevisiae. The two single deletion strains differed in terms of cell wall thickness and xylanase B secretion. Transcription levels of β-1,3-glucanosyltransferase genes and genes related to protein secretion and transport were assayed. The β-1,3-glucanosyltransferase genes displayed transcription complementation in the cell wall synthesis process. KlGAS1-1 and KlGAS1-2 affected transcription levels of secretion- and transport-related genes. Differences in protein secretion ratio among the three deletion strains were associated with changes of transcription levels of secretion- and transport-related genes. Our findings indicate that KlGAS1-1 deletion is an effective tool for enhancing industrial-scale heterologous protein secretion in K. lactis.

Polyvinylchloride Ultrafiltration Membranes Modified with Different SiO2 Particles and Their Antifouling Mechanism for Oil Extraction Wastewater

Zhao, Fang-Bo,Yu, Zhen-Jiang,Park, Hee-Deung,Liu, Xu-Yang,Song, Xiang-Ru,Li, Zhan-Shuang American Society of Civil Engineers 2015 Journal of environmental engineering Vol.141 No.8

Establishment of a novel myocarditis mouse model based on cyclosporine A

Zhao Tian Hao,Jiang Yi Xuan,Chen Kai Qin,Qiu Dan,Xu Yan Zhe,Ye Chun,Ren Ting,Zhang Bo,Dai Bin,Hu Jue,Lu Jun,Zhou Fang Liang,Xiao Rong,Lu Fang Guo,Wei Ke 한국유전학회 2022 Genes & Genomics Vol.44 No.12

Background: Myocarditis is a myocardial injury that can easily cause adolescent death. Traditional research models of animal invasion with viral components, lipopolysaccharide (LPS) or porcine myocardial myosin, among others, have the shortcomings of potential biological safety hazards and high animal mortality. Objective: To explore the construction of a novel myocarditis model with cyclosporine A and the potential genes and pathways associated with it. Methods: BALB/c mice were used in this study, and cyclosporin A and LPS were injected into the peritoneal cavity of mice. The successful establishment of the model was assessed by detecting serum myocardial injury markers and inflammatory factors levels, HE, IHC staining, and RT-qPCR methods. Key genes were obtained using the GSE35182 dataset from the GEO database and validated with the RT-qPCR method. Results: We found that a large number of inflammatory cells infiltrated the myocardium of mice in each group of Cyclosporin A constructed model, while the expression of inflammatory factor indicators was increased, and this model has the characteristics of high degree of local inflammation in myocardial tissue, low mortality, and safe and non-toxic treatment. Using GSE35182 data, we selected 18 Hub genes and validated Hub genes in myocardial tissue with RT-qPCR and found that multiple signaling pathways such as Toll-likereceptor signaling pathway(TLRs), Rap1 signal pathway(Rap1), and Chemokine signaling pathway may be involved in the development of myocarditis. Conclusion: Cyclosporin A can construct a new myocarditis model, and TLRs, Chemokines and Rap1 signaling pathways may be the core pathways of myocarditis.

Prediction Model of Austenite Growth and the Role of MnS Inclusions in Non- Quenched and Tempered Steel

Bo Jiang,Meng Wu,He Sun,Zhilin Wang,Zhigang Zhao,Yazheng Liu 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.1

The austenite growth behavior of non-quenched and tempered steels (casted by continuous casting and moldingcasting processes) was studied. The austenite grain size of steel B casted by continuous casting process is smallerthan that of steel A casted by molding casting process at the same heating parameters. The abnormal austenitegrowth temperature of the steels A and B are 950 °C and 1000 °C, respectively. Based on the results, the models forthe austenite grain growth below and above the abnormal austenite growth temperature of the investigated steelswere established. The dispersedly distributed fine particles MnS in steel B is the key factor refining the austenitegrain by pinning the migration of austenite grain boundary. The elongated inclusions MnS are ineffective in preventingthe austenite grain growth at high heating temperature. For the non-quenched and tempered steel, the continuouscasting process should be adopted and the inclusion MnS should be elliptical, smaller in size anddistributed uniformly in order to refine the final microstructure and also improve the mechanical properties.

Carbohydrate metabolism genes dominant in a subtropical marine mangrove ecosystem revealed by metagenomics analysis

Huaxian Zhao,Bing Yan,Shuming Mo,Shiqing Nie,Quanwen Li,Qian Ou,Bo Wu,Gonglingxia Jiang,Jinli Tang,Nan Li,Chengjian Jiang 한국미생물학회 2019 The journal of microbiology Vol.57 No.7

Mangrove sediment microorganisms play a vital role in the energy transformation and element cycling in marine wetland ecosystems. Using metagenomics analysis strategy, we compared the taxonomic structure and gene profile of the mangrove and non-mangrove sediment samples at the subtropical estuary in Beibu Gulf, South China Sea. Proteobacteria, Bacteroidetes, and Firmicutes were the most abundant bacterial phyla. Archaeal family Methanosarcinaceae and bacterial genera Vibrio and Dehalococcoides were significantly higher in the mangrove sediments than in the nonmangrove sediments. Functional analysis showed that “Carbohydrate metabolism” was the most abundant metabolic category. The feature of carbohydrate-active enzymes (CZs) was analyzed using the Carbohydrate-Active EnZymes Database. The significant differences of CZs between mangrove and non-mangrove sediments, were attributed to the amounts of polyphenol oxidase (EC 1.10.3.-), hexosyltransferase (EC 2.4.1.-), and β-N-acetylhexosaminidase (EC 3.2.1.52), which were higher in the mangrove sediment samples. Principal component analysis indicated that the microbial community and gene profile between mangrove and non-mangrove sediments were distinct. Redundancy analysis showed that total organic carbon is a significant factor that affects the microbial community and gene distribution. The results indicated that the mangrove ecosystem with massive amounts of organic carbon may promote the richness of carbohydrate metabolism genes and enhance the degradation and utilization of carbohydrates in the mangrove sediments.

Partial-state Feedback Stabilization for a Class of Generalized Nonholonomic Systems with ISS Dynamic Uncertainties

Yan Zhao,Chunxiao Wang,Jiang-Bo Yu 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.1

In this paper, the problem of global stabilization control via partial-state feedback is addressed for a class of uncertain nonholonomic systems with the disturbed virtual control directions. The investigated nonholonomic system has the input-to-state stable (ISS) dynamic uncertainties. The small gain theorem and the changing supply function technique are employed to derive a global stabilization controller. Additionally, we develop a switching control strategy in order to get around the smooth stabilization burden associated with nonholonomic systems. The simulation results illustrate the efficacy of the presented algorithm.

Robust Output Tracking Control for a Class of Uncertain Nonlinear Systems Using Extended State Observer

Yan Zhao,Jiang-Bo Yu,Jie Tian 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.3

This paper investigates the global robust output tracking control problem via output feedback for a classof nonlinear uncertain systems with the integral input-to-state stable (iISS) dynamic uncertainties. By performingthe coordinates transformation and employing an extended state observer (ESO), it can be seen that the robust setpointtracking control is well solved. The proposed control strategy simplifies the control design procedure. Thiscontrol scheme finds its application in the mass-spring mechanical system. It is shown that the asymptotic trackingcontrol for any desired displacement can be achieved in the mass-spring mechanical system in the case of unknownparameters. The simulation results demonstrate that the proposed control scheme has a better tracking performance. This verifies the effectiveness of the developed method.