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Jiao Yifan,Zhang Sihui,Yang Jing,Lai Xin-He,Dong Kui,Cheng Yanpeng,Xu Mingchao,Zhu Wentao,Lu Shan,Jin Dong,Pu Ji,Huang Ying,Liu Liyun,Wang Suping,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.2
Two facultatively anaerobic, short rod-shaped, non-motile, Gram-stain-positive, unknown bacterial strains (JY-X040T and JY-X174) were isolated from fluvial sediments of Tongtian River in Yushu Tibetan Autonomous Prefecture, Qinghai province, China. Cells formed translucent, gray, round and convex colonies, with a diameter of less than 0.5 mm after 5 days of incubation at 30°C on brain heart infusion-5% sheep blood agar. The 16S rRNA gene sequence similarity between strain JY-X040T and Fudania jinshanensis 313T is 93.87%. In the four phylogenetic trees constructed based on the 16S rRNA gene and 423 core genes, the two isolates form an independent branch, phylogenetically closest to F. jinshanensis 313T, but could not be classified as a member of the genus Fudania or any other genus of the family Arcanobacteriaceae. The DNA G + C content of strain JY-X040T was 57.8%. Calculation results of average nucleotide identity, digital DNADNA hybridization value and amino acid identity between strain JY-X040T and F. jinshanensis 313T are 69.9%, 22.9%, and 64.1%. The major cellular fatty acids were C16:0 (23%) and C18:1ω9c (22%). The cell-wall peptidoglycan type was A5α (L-Lys-L-Ala-L-Lys-D-Glu). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and four unidentified components. The whole-cell sugars contained rhamnose and ribose. MK-10(H4) was the sole respiratory quinone. The minimum inhibitory concentration of streptomycin was 32 μg/ml. All physiological, biochemical, chemotaxonomic and genomic characteristics support that strains JY-X040T and JY-X174 represent members of a novel species in a new genus, Changpingibacter yushuensis gen. nov., sp. nov. The type strain is JY-X040T (GDMCC 1.1996T = KCTC 49514T).
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Huang Yuyuan,Jiao Yifan,Zhang Sihui,Tao Yuanmeihui,Zhang Suping,Jin Dong,Pu Ji,Liu Liyun,Yang Jing,Lu Shan 한국미생물학회 2023 The journal of microbiology Vol.61 No.4
Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37′13″N, 96°05′37″E) in July 2019. Both strains were shown to grow at 15–35 °C and pH 7.0–10.0, and in the presence of 0–6.0% (w/v) NaCl. The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6–98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5–98.6%) and O. murale 01-Gi-040T (98.3–98.5%). The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0–23.9% and 70.8–80.4%, respectively, all below the respective recommended 70.0% and 95–96% cutoff point. Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9. Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids. β-cryptoxanthin ( C40H56O) can be extracted from strain JY.X270T, and its content is 6.3 μg/ml. Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp. nov. is proposed (type strain JY.X270T = CGMCC 1.19147T = JCM 34882T).
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Ma Shili,Xie Liquan,Ji Yifan,Su Tsung-chow 대한조선학회 2022 International Journal of Naval Architecture and Oc Vol.14 No.1
Suction caissons are widely used as a promising alternative foundation for offshore structures under variable offshore environments. In this paper, a three-dimensional finite-element model which takes into account soil inelasticity was used to study the seismic behavior of a suction caisson in clay taken from an offshore wind farm in the Hebei province of China. The results showed that the maximum acceleration of the soil increases with increasing depth. There is a distinct amplification for relatively low frequencies when seismic waves propagate to the mudline. It was assumed that the rotation center of suction caisson under seismic conditions was virtually existent. The rotation center was obtained by the proposed formula to explore the movement positions of the suction caisson. The time of the peak of lateral displacement of the lid did not synchronize with that of the changing direction of displacement of the rotation center, indicating that the displacement of the lid can not be a comprehensive assessment of the position state of suction caissons. The point mass at the top of the wind turbine tower was responsible for the increase of vibration amplitude of displacement.
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