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Seokhun Lee(이석훈),Seunggyu Kim(김승규),Jessie Sungyun Jeon(전성윤) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.4
Wet Age-related macular degeneration(AMD), the leading cause of blindness, is a severe ophthalmic disease developed in the outer blood-retinal barrier(oBRB), composed of two cells, the retinal pigment epithelium(RPE) and the choriocapillaris endothelial cell(CCE). Usually, oBRB sustains homeostasis, however, if it is broken for unknown reasons, Choroidal neovascularization(CNV) would develop. Unfortunately, the pathogenesis is still unclear and the risk of the only effective therapy (Anti-VEGF injection) has been consistently argued. Also, oBRB is hard to observe in vivo, and in vitro models have been struggled to mimic physiological features properly. Here, we produced the advanced oBRB model, which has proper maturation features. And using our model, we induced the CNV with chronic stress, without any exogenous cytokines. To enhance the similarity, the distance between two cells, which is important for cell communications, was tuned to the several micrometers. Also CCE was vascularized with perfusable lumen. To build this model, using microfluidic chip, HUVEC embedded fibrin gel was injected into the gel layer, and the ARPE-19 was coated directly on the gel layer. After 4 days of incubation, HUVEC finished the vascularization, and ARPE-19 constructed the confluent monolayer. Similar to the animal wet AMD experiments, the CNV disease model was incubated in hypoxia circumstance, and it showed distinguishable upregulated vascularization. We believe our model can help the research to reveal the wet AMD pathogenesis.