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Sejong Oh,Jeongran Lee,Gi An Lee,Jae Young Song,Tsvetelina,Lilia,Yeon Gyu Kim,Ju Hee Lee,Myung Chul Lee 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
The amount of genetic variability of a species is essential for its survival and adaptation in different environments, and studies of genetic diversity using molecular markers are necessary to understand the genetic structure of a population and to orientate effective strategies of germplasm conservation. The aim of current study was to determine the SSR markers that can be used rapidly and reliably to evaluated the pepper of Bulgaria landraces, and applied the markers to assement of introduce genetic diversity of the pepper germplasm. We used 22 polymorphic microsatellite markers to analysis of genetic diversity within 61 pepper collection of Bulgaria landraces germplasm, all SSR primers pairs produced 80 polymorphic and reproducible amplification fragments. An average value of polymorphic information contents (PIC) were 0.334 with a range of 0.061 to 0.63. The mean values of observed (HO) and expected heterozygosity (HE) were 0.383 and 0.154, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into three distinct groups, which was the landrace, moderate and wilde type, genetic distance (GD) value was 0.540. An average day of flowering time was 53 days with a range of 45 to 60 days. The everage od fruit length and width were 9.38cm with a range 2.1 to 23.6cm, and 3.51cm with a range 0.6 to 8.9cm, respectively. Molecular data were complemented with morphological measurements according to the descriptor list for the pepper collection of Bulgaria landraces germplasm.
Lee Jeongran,Lee Keunpyo,Kang Kyung-Ho,Oh Jeong Ho,Mamadou Sock,Baboucarr Manneh,Lee Sang Bok 한국국제농업개발학회 2022 한국국제농업개발학회 학술대회 Vol.2022 No.09
Sustainable capacity building of the rice value chain in Africa is essential in achieving the rice self-sufficiency and poverty alleviation. The breeding capacity enhancement has also been at the heart of AfricaRice’s mandate. Therefore, the Korea-Africa Food and Agriculture Cooperation Initiative (KAFACI) has concentrated breeding capacity enhancement for young breeders or breeding technicians of national research institutes in the KAFACI member countries. Four-month trainings have been conducted at the Regional Training Center located in Saint Louis, Senegal. The actual practice training is as follows; First is to learn practical skills and techniques of key aspects of rice breeding which are field preparation and layout, sowing, transplanting, fertilizer application, weed control, water management, hybridization/crossing, selection, yield and yield components determination, and rice palatability testing. Second is to learn some theoretical aspects of plant breeding through lectures including basic principles of breeding, evaluation for biotic and abiotic stresses, and statistical analysis. Third is to learn about the accurate use of field and lab equipment for rice breeding. Forth is to learn about proper scientific reporting and presentation. The courses were organized twice per year following two rice cultivation seasons in Senegal. From 2019, a total of 24 trainees participated in the 4 month courses. We believe they have acquired the basic knowledge and skills to implement rice breeding activities indpendently in their countries.
Lee Jeongran,Lee Keunpyo,Kang Kyung-Ho,Oh Jeong Ho,Mamadou Sock,Baboucarr Manneh,Lee Sang Bok 한국국제농업개발학회 2022 한국국제농업개발학회 학술대회 Vol.2022 No.09
The Korea-Africa Food and Agriculture Cooperation Initiative (KAFACI) has been cooperating with AfricaRice since 2014 for the eradication of hunger and poverty in Africa through sustainable agriculture and its industrialization. At first, rice breeding activities for staple food production started with KAFACI member countries by sharing high-yielding Tongil-type rice breeding lines and varieties. The Africa Rice Development Partnership project entitled “Enhancement of high-yielding rice germplasm and breeding capacity of rice producing countries in Africa” is implemented for the period from 2016 to 2025 with three phases. The actual rice breeding activities have been conducted by applying anther culture haploid breeding technology in the laboratory and field of AfricaRice Saint-Louis center. The 1,547 elite lines selected through participatory variety selection were distributed to 21 national breeders of KAFACI member countries. These lines derived from the 1,181 cross combination and 34,616 breeding lines on Korea and AfricaRice germplasm crossed. Among them 11 new Tongil-type rice varieties have been selected and registered in the national variety catalogue of Senegal, Mali, Rwanda, Malawi, and Tanzania.
Se-Jong Oh,Jae-Young Song,Jeongran Lee,Gi-An Lee,Ho-Cheol Ko,T. Stoilova,L. Krasteva,Yeon-Gyu Kim,Ju-Hee Rhee,Jae-Gyun Gwag,Na-Young Ro,On-Sook Hur,Myung-Chul Lee 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.5
본 연구는 농업유전자원센터에 보존되어있는 불가리아 재래종 고추 유전자원 61 점을 대상으로 농업형질을 조사하고, 22개의 분자마커(SSR marker)를 이용하여 불가리아 재래종 고추 유전자원의 유전적 다양성 및 집단분석을 통하여, 자원보존 및 효율적인 작물 육종을 위한 기초정보를 제공하고자 본실험을 수행하였으며 결과는 다음과 같다. 1. 파종 후 발아까지 소요일수는 최소 11일에서 최대 26 일,평균 16.9 일이었고 개화 소요일수는 최소 48일, 최대 65일, 평균 56.9일이었으며 성숙까지의 소요일수는 최소 73일, 최대 98일, 평균 90.7일이었다. 2. 농업형질의 특성을 바탕으로 PCA 분석을 이용하여 불가리아 고추의 다양성을 분석한 결과, 파종 후 개화까지의 소요일수에 따라 조생종, 중생종, 만생종 3개의 그룹으로 나눌 수있었다. 3. 61점의 고추자원에 대하여 22개 SSR 마커에 의해 나타난 대립유전자 (allele)수는 총 82개였다. 마커당 평균 allele수는 3.7 개였고, allele 수의 범위는 2개에서 5개로 확인되었다. 유전적 다양성을 나타내는 PIC 값의 범위는 0.061-0.636이었으며 평균 PIC 값은 0.349로 확인되었다. 4. 분자마커(SSR)를 이용하여 UPGMA, PCoA, STUCTURE 분석을 통한 고추의 다양성 및 집단 구조를 분석한 결과, 3개의 그룹으로 나뉘어졌다. 결론적으로, 농업형질 특성을 바탕으로 한 불가리아 재래종고추의 다양성과 분자학적 특성을 이용한 다양성 결과와는 차이가 있었다. The amount of genetic variability of a species is essential for its survival and adaptation in different environments, and studies of genetic diversity using molecular markers are necessary to understand the genetic structure of a population and to orientate effective strategies of germplasm conservation. The aim of current study was to determine the SSR markers that can be used rapidly and reliably to evaluated the pepper of Bulgaria landraces, and applied the markers to assessment of introduce genetic diversity of the pepper germplasm. We used 22 polymorphic microsatellite markers to analysis of genetic diversity within 61 pepper collection of Bulgaria landraces germplasm, all SSR primers pairs produced 82 polymorphic and reproducible amplification fragments. Results showed a relatively low level of genetic diversity among pepper accessions from Bulgaria for both molecular and morphological markers. An average value of polymorphic information contents (PIC) were 0.334 with a range of 0.061 to 0.63. The mean values of observed (HO) and gene diversity (GD) were 0.383 and 0.154, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into three distinct groups according to genetic difference among the accessions. An average day of flowering time was 56.9 days with a range of 48 to 65 days. The average of fruit length andwidth were 6.9cm with a range 2.6 to 13.9cm, and 1.9cm with a range 0.5 to 4.2cm, respectively.
Taxonomic Review of the Genus Echinochloa in Korea (I)
Jeongran Lee,Chang-Seok Kim,In-Yong Lee 한국잡초학회·한국잔디학회 2014 Weed & Turfgrass Science Vol.3 No.3
The genus Echinochloa (L.) P. Beauv. comprised of approximately 30-40 species in the tropical and warm temperate regions of the world, including numerous interspecific and intraspecific types which make the genus difficult to identify. As an attempt to identify the species within the genus easier, the taxonomy of the genus Echinochloa, Poaceae in Korea was reviewed on the basis of sequencing data derived from nuclear ribosomal DNA internal transcribed spacer (ITS) and external transcribe spacer and chloroplast DNA trnL intron, trnL-F intergenic spacer and matK regions using a total of 46 accessions representing all the species in Korea. The results of maximum parsimony found separate lineage comprised of E. colona and E. frumentaceae which are not Korean species, but no resolution within Korean Echinochloa species, supporting the suggestion of Yamaguchi group that E. crus-galli, E. oryzoides, and E. esculenta should be considered to belong to the same species. However, the relationship between these three species and the other species, i.e. E. oryzicola should be better understood with more detail studies.