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Jeong, Soon-Jeong,Kim, Sung-Shin,Bae, Chun-Sik,Park, Jin-Ju,Choi, Baik-Dong,Wang, Guanlin,Jung, Myung-Ju,Jang, Hyun-Sun,Kim, Byung-Ock,Lim, Do-Seon,Cho, Young-Sik,Jeong, Moon-Jin UNKNOWN 2012 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.29 No.2
<P>Secretory leukocyte protease inhibitor (SLPI) and estrogen promote wound healing through a decrease in the excessive inflammatory response, accelerating re-epithelialization and increasing the amount of collagen deposition. The excessive administration of estradiol valerate (EV) using hormonal therapy decreases the concentration of estrogen abruptly and induces the polycystic ovary syndrome (PCOS). In this study, the PCOS rat skin wound area was wider than that of the normal groups and the rate of keratinocyte migration in PCOS was lower than the normal group. The numbers of inflammatory cells and macrophages recruited in the PCOS group were larger than that of the normal group. More collagen was deposited in the healing area of the normal group than in the PCOS group. The level of SLPI expression was higher in the PCOS group than the normal group after wounding, with the exception of the epithelium. On the other hand, mRNA and protein expression levels of transforming growth factor-β1 (TGF-β1) were lower in the PCOS group than in the normal group. Matrix metalloproteinase-2 (MMP-2) and MMP-9 levels in the PCOS group were significantly lower than that of the normal group. Therefore, increased SLPI in PCOS skin wounds may help prevent an excessive inflammatory response and aberrant collagen deposition but not are sufficient to accelerate PCOS skin wound healing, suggesting that SLPI may act as a local rather than a systemic modulating molecule in PCOS rat skin wounds.</P>
Ock, Sun-A,Baregundi Subbarao, Raghavendra,Lee, Yeon-Mi,Lee, Jeong-Hyeon,Jeon, Ryoung-Hoon,Lee, Sung-Lim,Park, Ji Kwon,Hwang, Sun-Chul,Rho, Gyu-Jin Hindawi Publishing Corporation 2016 Stem cells international Vol.2016 No.-
<P>Mesenchymal stromal/stem cells (MSCs) demonstrate immunomodulation capacity that has been implicated in the reduction of graft-versus-host disease. Accordingly, we herein investigated the capacity of MSCs derived from several tissue sources to modulate both proinflammatory (interferon [IFN] <I>γ</I> and tumor necrosis factor [TNF] <I>α</I>) and immunosuppressive cytokines (transforming growth factor [TGF] <I>β</I> and interleukin [IL] 10) employing xenogeneic human MSC-mixed lymphocyte reaction (MLR) test. Bone marrow-derived MSCs showed higher self-renewal capacity with relatively slow proliferation rate in contrast to adipose-derived MSCs which displayed higher proliferation rate. Except for the lipoprotein gene, there were no marked changes in osteogenesis- and adipogenesis-related genes following in vitro differentiation; however, the histological marker analysis revealed that adipose MSCs could be differentiated into both adipose and bone tissue. TGF<I>β</I> and IL10 were detected in adipose MSCs and bone marrow MSCs, respectively. However, skin-derived MSCs expressed both IFN<I>γ</I> and IL10, which may render them sensitive to immunomodulation. The xenogeneic human MLR test revealed that MSCs had a partial immunomodulation capacity, as proliferation of activated and resting peripheral blood mononuclear cells was not affected, but this did not differ among MSC sources. MSCs were not tumorigenic when introduced into immunodeficient mice. We concluded that the characteristics of MSCs are tissue source-dependent and their in vivo application requires more in-depth investigation regarding their precise immunomodulation capacities.</P>
OCK, Chan Young,LIM, Yun Jeong,KIM, Yoon Jae,CHUNG, Jun Won,KWON, Kwang An,KIM, Ju Hyun,HAHM, Ki Baik Blackwell Publishing Asia 2011 Journal of digestive diseases Vol.12 No.2
<P><B>OBJECTIVE: </B> Heat shock proteins (HSPs) are crucial for the maintenance of cellular integrity during normal cell growth as well as pathophysiological conditions. While acting as molecular chaperones with their folding activities, HSPs play a cytoprotective role to rescue epithelial cells from several gastric damages including non‐steroidal anti‐inflammatory drugs (NSAIDs) and <I>Helicobacter pylori</I>. Since the exact relationship between HSP27 phosphorylation and biological function remains unknown in NSAID‐induced gastropathy, we hypothesized that revaprazan, a novel acid pump antagonist, can secure significant cytoprotection from NSAID damages through HSP27 accentuation.</P><P><B>METHODS: </B> We evaluated protective actions of revaprazan against either <I>in vivo</I> animal model of indomethacin induced gastropathy or <I>in vitro</I> cell model focused on HSP27 expression and activation.</P><P><B>RESULTS: </B> Indomethacin induced significant cytotoxicities accompanied with phosphorylated HSP27 and attenuated levels of HSP27 in the <I>in vitro</I> cell experiment and revaprazan administration protected stomach from indomethacin‐induced gastric damages in accordance with HSP27 dephosphorylation in the <I>in vivo</I> animal experiment. HSP27 siRNA abolished these cytoprotective privileges of revaprazan. Indomethacin, 40 mg/kg, <I>po</I>, administration provoked significant levels of gastric damages accompanied with decrement in HSP27, while rats administrated with revaprazan prior to indomethacin imposed the accentuation of HSP27, of which levels were significantly correlated with the prevention of the indomethacin‐induced gastric damages.</P><P><B>CONCLUSION: </B> HSP27 phosphorylation with resultant decrease in HSP27 level was one of the mechanisms of indomethacin‐induced cytotoxicity, of which post‐translational modifications were prevented with revaprazan administration in the presence of indomethacin. Therefore, acid pump antagonist could be a choice for the prevention of NSAID‐induced gastropathy backed up with distinctive cytoprotective action beyond acid suppressor.</P>
CCI엔진연소의 운전영역확장을 위한 성층화효과에 대한 연구
임옥택(Ock Taeck Lim),표영덕(Young Dug Pyo),정수진(Soo-Jin Jeong) 한국연소학회 2010 KOSCOSYMPOSIUM논문집 Vol.- No.41
The purpose of this study is to investigate the mechanism and potential of stratified charge for reducing PRR on HCCI combustion. The numerical calculation with multi-zones model is run to know the potential of stratified charge for reducing PRR. DME is used as a fuel. As a result, following conclusions were obtained. 1) Stratified charge makes the gas temperature difference before reaction start by the differences of initial gas temperature, equivalence ratio and EGR ratio. As a result, the PRR reduction is occurred by the difference of reaction start timing. 2) gain the PRR reduction effect of 50%, temperature difference of 20K in case of thermal stratification, equivalence ratio difference of 0.16 in case of mixing stratification, EGR ratio difference of 15% in case of EGR stratification are needed. 3) When thermal, mixing and EGR stratifications are combined, “in case of relatively rich mixture with high temperature and relatively lean mixture with low temperature” and “in case of relatively rich EGR with low temperature and relatively lean EGR with high temperature” show the greatest potential for reducing PRR.
Lee, Jeong-Hyeon,Lee, Won-Jae,Jeon, Ryoung-Hoon,Lee, Yeon-Mi,Jang, Si-Jung,Lee, Sung-Lim,Jeon, Byung-Geun,Ock, Sun-A,King, W. Allen,Rho, Gyu-Jin Mary Ann Liebert 2014 Cellular reprogramming Vol.16 No.6
<P>The present study compared the potential of porcine bone marrow mesenchymal stem cells (pBMSCs) at different passages as nuclear transfer (NT) donors and the developmental efficiency of NT embryos from donor cells transfected with/without Oct4 and Sox2. Early-passage pBMSCs showed higher proliferation and expression of Oct4 and Sox2 and differentiation potential into mesenchymal lineages than middle- and late-passage pBMSCs. Cleavage rate did not differ among pBMSCs at different passages, but NT embryos with early-passage pBMSCs and middle-passage pBMSCs transfected with Oct4 (Oct4-pBMSCs) had significantly (p<0.05) higher blastocyst development than those with middle-passage pBMSCs. The incidence of apoptotic bodies in NT blastocysts from late-passage pBMSCs and Sox2-transfected middle-passage pBMSCs (Sox2-pBMSCs) was significantly (p<0.05) higher than others. The transcriptional levels of Oct4, Sox2, Nanog, Cdx2, Dnmt3a, and Igf2r genes were significantly (p<0.05) higher in Oct4- and Sox2-pBMSCs NT embryos. Middle-passage pBMSCs NT embryos revealed lower transcriptional levels of Bcl2 than others, except Sox2-pBMSCs NT embryos. The transcriptional level of Bax increased gradually in NT embryos derived from pBMSCs following extended passages and was significantly (p<0.05) higher in Sox2-pBMSCs NT embryos. Our results demonstrated that early-passage pBMSCs are more potent in expressing transcription factors and displayed higher differentiation ability, and middle-passage pBMSCs transfected with Oct4 improved the developmental efficiency of NT embryos, suggesting that high Oct4 expression cells are more efficient as NT donors.</P>
HCCI엔진연소의 압력상승률 저감의 메카니즘에 대한 이해
임옥택(Ock Taeck Lim),정수진(Soo-Jin Jeong),표영덕(Young Dug Pyo) 한국자동차공학회 2010 한국자동차공학회 학술대회 및 전시회 Vol.2010 No.11
HCCI engine is able to achieve low NOx and particulate emissions as well as high efficiency. However, its operation range is limited by the knocking at high load, which results from an excessive . To solve the knocking problem, the PRR must be reduced, and stratified charge has been suggested. It is the method to disperse the auto-ignition timing at each local gas in combustion chamber by using thermal, mixing and stratifications. The purpose of this study is to investigate the mechanism and potential of stratified charge for reducing PRR on HCCI combustion. The numerical calculation with multi-zones model is run to know the potential of stratified charge for reducing PRR. DME is used as a fuel. As a result, following conclusions were obtained. 1) Stratified charge makes the gas temperature difference before reaction start by the differences of initial gas temperature, equivalence ratio and EGR ratio. As a result, the PRR reduction is occurred by the difference of reaction start timing. 2) gain the PRR reduction effect of 50%, temperature difference of 20K in case of thermal stratification, equivalence ratio difference of 0.16 in case of mixing stratification, EGR ratio difference of 15% in case of EGR stratification are needed. 3) When thermal, mixing and EGR stratifications are combined, “in case of relatively rich mixture with high temperature and relatively lean mixture with low temperature” and “in case of relatively rich EGR with low temperature and relatively lean EGR with high temperature” show the greatest potential for reducing PRR.
PLGA 담체의 제조방법에 따른 골수간엽줄기세포의 골분화 유도
소정원 ( Jeong Won So ),김순희 ( Soon Hee Kim ),백미옥 ( Mi Ock Baek ),임지예 ( Ji Ye Lim ),노혜원 ( Hye Won Roh ),이나리 ( Na Ri Lee ),유규하 ( Gyu Ha Ryu ),조양하 ( Yang Ha Cho ),이승진 ( Seung Jin Lee ),강길선 ( Gilson Khang ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.1
A key component in tissue engineering for bone regeneration is the scaffold that serves as a template for cell interaction and the formation of bone-extracellular matrix to provide structural support to the newly formed tissue. In this study, poly(L-Lactide-co-glicolide)(PLGA) scaffolds were prepared by solvent casting/salt-leaching, ice particle-leaching and gas-forming/salt-leaching. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazole- 2-yl)-2,5-diphenyltetrazolium-bromide(MTT) test. Osteogenic differential cells were analyzed by ALP activity. Through RT-PCR analysis, we observed ALP and osteocalcin m RNA expression. Scaffold with BMSCs were implanted into the back of athymic nude mouse to observe in vivo behavior. Thin section were cut from paraffin embedded tissues and histological sections were stained by trichrome, immunohistochemical, von kossa staining. The cell proliferation and ALP activity of BMSCs in the scafoolds was the highest in the scaffolds using solvent casting/salt-leaching method. In RT-PCR results, in the all type of scaffolds observed specific gene expression of ALP, osteocalcin. By histological staining, we could observed collagen and calcification region in scaffolds by solvent casting/salt leaching method. Thus Solvent casting/salt-leaching method provided highly porous structure with good interconnections between each pores, which can support the surface of cell proliferation, differentiation. This result indicates that solvent casting/salt-leaching is useful for osteogenesis of BMSCs.