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      • Non-enzymatic biosensing of glucose based on silver nanoparticles synthesized from Ocimum tenuiflorum leaf extract and silver nitrate

        Dayakar, T.,Venkateswara Rao, K.,Park, Jinsub,Sadasivuni, Kishor Kumar,Ramachandra Rao, K.,Jaya rambabu, N. Elsevier 2018 Materials chemistry and physics Vol.216 No.-

        <P><B>Abstract</B></P> <P>An enzyme-free glucose sensor developed based on silver nanoparticles (Ag NPs) via bio-mediated route using <I>Ocimum tenuiflorum</I> leaves extract. The Ag NPs were characterized by X-ray diffraction (XRD), ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, Particle size analyzer (PSA), Scanning electron microscopy (SEM), Energy-dispersive X-ray (EDAX) spectroscopy, and Transmission electron microscopy (TEM), to study structural, optical and morphological properties. The electrocatalytic activity of Ag NPs towards the oxidation of glucose in the 0.1 M NaOH electrolyte solution was analyzed. The Ag NPs was coated on Glassy carbon electrode (GCE) and reports indicating the outstanding capability such as high sensitivity (895.8 μAmM<SUP>−1</SUP>cm<SUP>−2</SUP>), linear range (1–8.9 mM), response time (<4s), low detection limit (0.0048 μM, S/N = 3), long-term stability, reproducibility, repeatability, and selectivity of the sensor. Therefore, the bio-synthesized Ag NPs can markedly helpful to fabricate non-enzymatic, sustainable, simple, low cost, and eco-friendly glucose monitoring devices.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ocimum tenuiflorum leaf extract and silver nitrate were used to prepare Ag NPs. </LI> <LI> Structural, optical and morphological properties of Ag NPs were studied. </LI> <LI> The GCE modified with Ag NPs showed a sensitivity of 895.8 μAmM<SUP>−1</SUP>cm<SUP>−2</SUP>. </LI> <LI> Bio-mediated synthesized NPs showed sustainable glucose sensing properties. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

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