http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Are Lumbar Fusion Guidelines Followed? A Survey of North American Spine Surgeons
Thiago S. Montenegro,Christopher Elia,Kevin Hines,Zorica Buser,Jefferson Wilson,Zoher Ghogawala,Shekar N. Kurpad,Daniel M. Sciubba,James S. Harrop 대한척추신경외과학회 2021 Neurospine Vol.18 No.2
Objective: To evaluate the use of guidelines for lumbar spine fusions among spine surgeons in North America. Methods: An anonymous survey was electronically sent to all AO Spine North America members. Survey respondents were asked to indicate their opinion surrounding the suitability of instrumented fusion in a variety of clinical scenarios. Fusion indications in accordance with North America Spine Society (NASS) guidelines for lumbar fusion were considered NASS-concordant answers. Respondents were considered to have a NASS-concordant approach if ≥70% (13 of 18) of their answers were NASS-concordant answers. Comparisons were performed using bivariable statistics. Results: A total of 105 responses were entered with complete data available on 70. Sixty percent of the respondents (n=42) were considered compliant with NASS guidelines. NASS-discordant responses did not differ between surgeons who stated that they include the NASS guidelines in their decision-making algorithm (5.10±1.96) and those that did not (4.68± 2.09) (p=0.395). The greatest number of NASS-discordant answers in the United States. was in the South (5.75±2.09), with the lowest number in the Northeast (3.84±1.70) (p< 0.01). For 5 survey items, rates of NASS-discordant answers were ≥40%, with the greatest number of NASS-discordant responses observed in relation to indications for fusion in spinal deformity (80%). Spine surgeons utilizing a NASS-concordant approach had a significant lower number of NASS-discordant answers for synovial cysts (p=0.03), axial low back pain (p<0.01), adjacent level disease (p<0.01), recurrent stenosis (p<0.01), recurrent disc herniation (p=0.01), and foraminal stenosis (p<0.01). Conclusion: This study serves an important role in clarifying the rates of uptake of clinical practice guidelines in spine surgery as well as to identify barriers to their implementation.
( Clayton P. Santiago ),( Laura N. Quick ),( James W. Wilson ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.11
The IncP plasmid R995 has been a useful selftransmissible, broad-host-range vector for a number of applications including the recombinase/conjugation-based cloning of large genomic DNA segments. However, R995 derivatives (or related plasmids) expressing a wide range of different resistance markers and Flp recombinase target sites do not exist in the literature. In addition, documented strategies for applying such plasmids in cloning applications that take advantage of conjugation for the convenient isolation and recovery of constructs are extremely limited. Here, we report a new series of R995 plasmids with alternative markers to increase options for applications in backgrounds already expressing resistance to a particular antibiotic(s). These R995 plasmids have been engineered to contain FRT sites that can be used for recombinase-based cloning. We demonstrate the utility of this approach by cloning 20 kb regions from the Salmonella Typhimurium and Escherichia coli genomes and by cloning DNA from an exogenous plasmid source. To our knowledge, this represents the first systematic engineering of an intact, self-transmissible IncP plasmid with a series of alternative antibiotic markers and FRT sites.
최명석,Ankush Gupta,서정화,S. Velmathi,James N. Wilson,Jong S. Park 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.1
In this study, we report the efficient identification of proteins using (6-hydroxy-2-naphthyl)ethenyl pyridinium dyes (HNEPs). These dyes exhibited characteristic fluorescence responses, showing enhanced fluorescence with a red shift in the case of bovine serum albumin. The nature of the interaction between the dye and the protein was examined using a combination of various spectroscopic techniques. A time-dependent density functional theory analysis supported the strong environment-dependent fluorescence emission properties of the dyes. The current investigation clearly demonstrates that a selective optical response HNEPs can find potential usefulness as bio-sensing probes.