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        Development of a conductivity-based photothermal absorbance detection microchip using polyelectrolytic gel electrodes

        Chun, Honggu,Dennis, Patty J.,Ferguson Welch, Erin R.,Alarie, Jean Pierre,Jorgenson, James W.,Ramsey, J. Michael Elsevier 2017 Journal of chromatography Vol.1523 No.-

        <P>The development and application of polyelectrolytic gel electrodes (PGEs) for a microfluidic photothermal absorbance detection system is described. The PGEs are used to measure changes in conductivity based on heat generation by analytes absorbing light and changing the solution viscosity. The PGEs are suitable for direct contact conductivity measurements since they do not degrade with exposure to high electric fields. Both a 2-electrode system with DC voltages and a 3-electrode system with AC voltages were investigated. Experimental factors including excitation voltage, excitation frequency, laser modulation frequency, laser power, and path length were tested. The limits of detection for the 3-electrode and 2-electrode systems are 500 nM and 0.55 nM for DABSYL-tagged glucosamine, respectively. In addition, an electrokinetic separation of a potassium, DABSYL-tagged glucosamine, Rhodamine 6G, and Rhodamine B mixture was demonstrated. (C) 2017 Elsevier B.V. All rights reserved.</P>

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        억새의 숙기별 영양가 변화에 관한 연구

        배동호,J . G . 웰츠 ( Dong Ho Bae,James G . Welch ) 한국축산학회 1979 한국축산학회지 Vol.21 No.6

        Transplanted Miscanthus sinessis was studied for the evaluation of nutritive value in relation to the stage of maturity. Plants were cut at eight stages of maturity beginning on June 9, and at ten-day intervals until August 23. The dry matter content of the plant was approximately constant until July 19, which was the early boot stage, and increased rapidly until August 23. The protein content decreased uniformly with each delay in cutting until July 29 which was the prebloom stage, and leveled off until August 23. The protein content until the prebloom stage was similar to that of timothy hay. The CWC and ADF contents increased fast in earlier stages of maturity and leveled off in later stages. While lignification accelerated in later stages of maturity. Until the early bloom stage, the CWC content of Miscanthus sinensis was not high compared to other grass hays. But after the midbloom stages, it was the same or a little higher `than coastal Bermuda grass pellets. A linear relationship (Y=-0.473x+94.3, R²= 0.986) between the in vitro DDM (Y) and cutting date (x) was found. The DDM declined at a rate of 0.47% per day for each day`s delay in harvesting after June There was no relationship between calcium and phosphorous content and cutting date. The average calcium content was lower than in the early bloom stage of timothy hay, and phosphorous content was similar to that of timothy hay.

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