난관배양액이 처녀발생유기된 돼지난포란의 체외발달에 미치는 영향

문승주,이경호,김호,김창렬,은대숙,김광현,나진수,김재홍 全南大學校 農業科學技術硏究所 1997 農業科學技術硏究 Vol.32 No.-

본 연구는 난관배양액이 돼지수정란의 체외발달에 미치는 효과를 규명키 위하여 수행하였다. 돼지 미성숙 난포란은 TCM-199, Ham's F-10 그리고 Whitten's 배양액에 10% 난포액과 호르몬(PMSG : 10IU/㎖, HCG : 10IU/㎖)을 첨가 20시간 배양하고 호르몬을 첨가하지 않는 배양액에서 20시간 추가 배양하여 총 40시간동안 배양하여 체외성숙을 유도하였다. 체외성숙후 0.1% hyaluronidase로 난구세포를 제거하고 15% FCS가 함유된 TCM-199으로 3회 세척하고 TCM-199에 15% FCS와 10% ethanol 혼합액에 세척한 난자를 옮겨 10분간 배양 처녀발생을 유기하였다. 처녀발생 6시간후 전핵형성율은 체외성숙배양액으로 TCM-199을 사용했을 때 56.4%, Ham's F-10의 경우 58.3%, Whitten's 배양액의 경우 74.0%를 보였다. 처녀발생 유기 48시간째 난할율은 TCM-199을 사용했을 때 45.7%, Ham's F-10에서 45.4%, Whitten's배양액에서 39.2%를 보였으며 세종류의 배양액에 POCM을 첨가 배양했을 때 TCM-199에 44.8%, Ham's F-10에서 45.4%, Whitten's배양액에서 43.7%로 나타났다. 처녀발생육 난자를 96시간 체외배양시킨 결과 상실배 발달율이 POCM을 첨가 했을 때 첨가하지 않은 시험구에 비하여 유의적으로 높았다(P<0.05) The effect of porcine oviductal conditioned medium(POCM) on in vitro development of chemically activated porcine oocytes was studied. Porcine oocytes were cultured in TCM-199, Ham's F-10 and Whitten's medium with hormonal supplements for 20h and 40h additional culture without hormonal supplements. After in vitro maturation, the denuded oocytes were washed 3 times with TCM-199 contaning 15%(v/v) ethanol to induce pathenogenetic activation. At 6h after activation, pronuclea formation rates were 56.4% in TCM-199, 59.3% in Ham's F-10 and 74.0% in Whitten's maturation medium. At 48h after activation, 45.7%, 45.4% and 39.2% of oocytes claved in TCM-199, Ham's F-10 and Whitten's culture medium, respectively. And 44.8%, 45.5% and 43.7% of oocytes were claved in TCM-199, Ham;s F-10 and Whitten's culture medium supplemented with POCM, respectively. The rates of moular were higher in culture medium with POCM than without POCM at 96h after activation.(P<0.05)

An Active Fraction on Phagocytosis of Murine Peritoneal Macrophage in Lithospermi Radix

Eun, J.-S 한국생약학회 1998 생약학회지 Vol.29 No.4

Ultraviolet photodissociation spectroscopy of cold, isolated adenine complexes with a potassium cation

Baek, J.Y.,Choi, C.M.,Eun, H.J.,Park, K.S.,Choi, M.C.,Heo, J.,Kim, N.J. North Holland 2015 Chemical physics letters Vol.635 No.-

We obtain the ultraviolet photodissociation spectrum of adenine complexes with K<SUP>+</SUP> ion stored in a cold ion trap. The spectrum near the origin band of the S<SUB>0</SUB>-S<SUB>1</SUB> transition exhibits well-resolved vibronic bands, all of which are assigned from a single isomer by UV-UV hole-burning (HB) spectroscopy. Comparing the spectrum with theoretical spectra, we determine the structure of the isomer, where K<SUP>+</SUP> is bound not to 9H-adenine (A9) but to 7H-adenine (A7). We suggest that K<SUP>+</SUP>A7 ions are formed in solution through tautomerization, for which the energy barrier varies greatly depending on the binding site of the K<SUP>+</SUP> ion on A9.

IMx Glycated Hemoglobin측정법에 관한 연구

황성준,박종성,이승관,이창규,류정록,변대훈,박은병 高麗大學校 倂設 保健大學 保健科學硏究所 1994 保健科學論集 Vol.20 No.1

Percent glycated hemoglobin(%GHb) is an indirect measure of the mean blood glucose level in an individual over the previous two to three months. A fully automated assay for quantitating %GHb in human whole blood has been developed using the Abbot IMx R analyzer. The assay is accomplished by specific solution phase tagging of glycated hemoglobin by a soluble polyanionic affinity reagent, and subsequent capture of the anionic complex by a cationic solid phase matrix. Both glycated hemoglobin(GHb) and total hemoglobin(Hb) are quantitated by measuring quenching of the fluorescence from an added dye by home. A correlation study was conducted accross IMx R instruments with fully automated HPLC (Hi-Auto A_(IC TM), Kyoto Daiiclii co.) as the reference assay. The correlation statistics obtained for 20 diabetic specimens were: IMx=0.84 HPLC+1.75, correlation coefficient= 0.969. Studies from linear relationship statistics had showed that there ere no evidence for difference between two methods. IMx glycated hemoglobin assay was don-sidered to be compatible with HPLC method.

Cu-Al-Ni계 초탄성 합금의 변태특성 및 변형거동에 미치는 미세조직의 영향

박정수,김경훈,은상원,조무현,장우양,양권승,강조원 조선대학교 생산기술연구소 2004 生産技術硏究 Vol.26 No.2

The effect of microstructure on transformation characteristics and deformation behavior in Cu-Al-Ni based superelastic alloy has been studied in alloy by using metallography, calorimetry, compression test and XRD. When the alloy betatized at the elevated temperature above 600℃, the r₂ phase completely dissolved into the matrix. The β₁' or r₁' martensites from β₁ patent phase was transformed in as-cast state but the parent phase stabilized by betatizing at 600℃. With increasing betatizing temperatures above 600℃. both₁' and r₁' martensites again transformed from β₁ parent phase and the heat flow of r₁' martensite was higher than that of β₁' martensite for reverse transformation. The compressed specimen, showing pseudoelastic behavior, was completely recovered upon unloading. However, the stress-strain curves for compression test were different by betatizing temperature; the stress-strain curves of the specimen betatized at 600℃ and 700℃ were linear but those of the specimen betatized at 800℃ and 900℃ were non-linear. The r₁' martensite was retained after compression test and the volume fraction of that increased with increasing betatizing temperature and strain.

Effect of Ethyl Alcohol Fraction of Cervus nippon on Mouse T-Lymphocyte

Jeon, K.-J 한국생약학회 1998 생약학회지 Vol.29 No.4

Development and Application of Efficacy Evaluation Techniques for Barrier Creams

Suh, D. H 大韓臨床藥理學會 1997 臨床藥理學會誌 Vol.5 No.1

난관액, 난관배양액 및 난포액이 돼지정자의 운동성과 수정능 획득에 미치는 영향

문승주,이경호,김호,은대숙,김창렬,김광현,나진수,김재홍 全南大學校 農業科學技術硏究所 1997 農業科學技術硏究 Vol.32 No.-

본 연구는 돼지 정자의 운동성과 첨체반응에 대한 난관액, 난관배양액 및 난포액의 효과를 조사하였다. 수정능획득배양액에 난관액(10%)을 첨가 공배양했을 때 정자의 운동성은 처리구에서 유의적으로 높았다(P<0.05). CTC 염색방법으로 정자의 첨체반응을 조사한 결과 난관액과 난포액이 첨가된 수정능획득 배양액에서 1.5, 3, 4.5시간동안 전배양을 실시하였을 때 정자의 첨체반응이 대조구에 비하여 유의적으로 높았으며(P<0.05), 난관배양액에서 1.5, 3, 4.5시간동안 전배양을 실시하였을때도 대조구에 비하여 정자첨체반응이 유의적으로 높았다(P<0.05). 이러한 연구결과는 난관과 난포액등에 함유되어있는 어떤인자가 정자의 운동성과 첨체반응율을 높힌다고 사료된다. The effects of oviductal fluid, oviductal conditioned medium and follicular fluid on porcine sperm mortility and capacitation were studied. The addition of oviductal fluid, oviductal conditioned medium and follicular fluid to the prefertilization medium increased sperm mortility(P<0.05). Chloroterracycline(CTC) flourescence patterns were used to determine incidence of acrosome reaction. The proportion of acrosome reaction increased when spermatozoa were exposed for 1.5, 3 and 4.5h to oviductal fluid and follicular fluid(P<0.05). In sperm-POCM(porcine oviductal conditioned medium) co-culture period of 4.5 h, acrosome reaction rates was higher than other groups(P<0.05). These results suggest that the factor(s) in secreation from oviduct and follicular fluid increased sperm mortility and acrosome reaction.

Evaluation of Pharmacokinetics and Skin Irritation of Transdermal Ketoprofen Plaster(Kefen[R]) in Healthy Volunteers

Yim, D. S 大韓臨床藥理學會 1996 臨床藥理學會誌 Vol.4 No.2

Moutan radix 의 mouse sarcoma 180 cell 로 유발한 mouse ascites cancer 에 대한 항암효과

반경녀(Kyeong N . Bahn),이은주(Eun J . Lee),양민석(Min S . Yang),김정옥(Jeong O . Kim),하영래(Yeong L . Ha) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.4

Anticarcinogenic activity of Moutan radix for mouse ascites cancer induced by mouse Sarcoma 180 (S-180) cells was investigated. Methanol extract of Moutan radix including other folk medicinal plants (Taxus cuspidata, Curcuma Tonga, Artemisia capillaris, Ligrstri fructus, and Liriope platyphylla) used to remedy or cure many chronic human diseases like cancer was fractionated into hexane, chloroform (CHCl₃), ethylacetate (EtOAc), and butanol (BuOH) fractions. Anticarcinogenic activity of the fractions, exhibited a strong cytotoxicity for L1210 and S-180 cells, was examined for mouse ascites cancer induced by S-180 cells. Male ICR mice (7 mice/treatment, 5∼6 weeks of age, 23±1 g) were injected i.p. with S-180 cells (1×10^7 cell/1 ㎖ PBS). One day later, each mouse was given 0.1 ㎖ of 10% DMSO containing sample (30 ㎍/g body weight) every day for 10 consecutive days. Control mice were only given 0.1 ㎖ S-180 cells and 0.1 ㎖ 10% DMSO. Mice treated with EtOAc fraction of Moutan radix showed 28.7 days of life, which is 167% of control mice`s life. Based on the dose-dependant experiment, mice treated with 30 ㎍ showed longer life relative to mice treated with other doses (5, 15, 60 ㎍) and mice treated with 60 ㎍ exhibited toxic symptoms. Body weight of mice treated with Moutan radix was significantly reduced relative to that of control mice (p$lt;0.05). GC-MS analysis in conjunction with silica-gel column chromatography revealed that the EtOAc fraction contained 2-methoxylphenol, benzoic acid, 1-(4-hydroxy-3-methoxyphenyl)ethanone, 8-methyl-2,4(1H,3H)pteridinedione and 2,5-furan-dicarboxylic dimethyl ester as regards to the anticarcinogenic property of the EtOAc fraction. These results suggest that Moutan radix might be included as an anticarcinogenic medicinal plant for treatment of ascites cancer.