http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : J. Hamlin (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
David J. Lemon,Matthew K. Kay,James K. Titus,April A. Ford,Wen Chen,LCDR Nicholas J. Hamlin,Yoon Y. Hwang 한국미생물학회 2019 The journal of microbiology Vol.57 No.6
Bacteriophage therapy was an ascendant technology for combating bacterial infections before the golden age of antibiotics, but the therapeutic potential of phages was largely ignored after the discovery of penicillin. Recently, with antibioticresistant infections on the rise, these phages are receiving renewed attention to combat problematic bacterial infections. Our approach is to enhance bacteriophages with antimicrobial peptides, short peptides with broad-spectrum antibiotic or antibiofilm effects. We inserted coding sequences for 1018, an antimicrobial peptide previously shown to be an effective broad-spectrum antimicrobial and antibiofilm agent, or the fluorescent marker mCherry, into the T7Select phage genome. Transcription and production of 1018 or mCherry began rapidly after E. coli cultures were infected with genetically modified phages. mCherry fluorescence, which requires a 90 min initial maturation period, was observed in infected cultures after 2 h of infection. Finally, we tested phages expressing 1018 (1018 T7) against bacterial planktonic cultures and biofilms, and found the 1018 T7 phage was more effective than the unmodified T7Select phage at both killing planktonic cells and eradicating established biofilms, validating our phage-driven antimicrobial peptide expression system. The combination of narrow-spectrum phages delivering relatively high local doses of broad-spectrum antimicrobials could be a powerful method to combat resistant infections. The experiments we describe prove this combination is feasible in vitro, but further testing and optimization are required before genetically modified phages are ready for use in vivo.
-
New materials for glass-ceramic X-ray storage phosphors
A. Edgar,M. Secu,S. Schweizer,J. -M. Spaeth,G. V. M. Williams,J. Hamlin 한국물리학회 2004 Current Applied Physics Vol.4 No.2-4
The development of transparent glass ceramics as X-ray storage phosphors and their advantages over their crystalline coun-terparts are briey reviewed. Measurements of conversion eciency, stimulation eciency, storage times, and optical transparencyare reported for the particular case of europium-doped uorobromozirconate glass, in which rubidium and lithium replace the usualsodium component fraction. Annealing at 280.C converts the as-prepared glass into a glassceramic which contains Rb2BaBr4 andRbBa2Br5 nano- and micro-crystallites. Only the RbBa2Br5 structure shows signicant photo-stimulated luminescence after X-irradiation, and the maximum storage phosphor eciency is 8% of that for the standard commercial storage phosphor BaFBr:Eu.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
