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Total Synthesis of Swinholide A: An Exposition in Hydrogen-Mediated C–C Bond Formation
Shin, Inji,Hong, Suckchang,Krische, Michael J. American Chemical Society 2016 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.138 No.43
<P>Diverse hydrogen-mediated C–C couplings enable construction of the actin-binding marine polyketide swinholide A in only 15 steps (longest linear sequence), roughly half the steps required in two prior total syntheses. The redox-economy, chemo- and stereoselectivity embodied by this new class of C–C couplings are shown to evoke a step-change in efficiency.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2016/jacsat.2016.138.issue-43/jacs.6b10645/production/images/medium/ja-2016-10645f_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja6b10645'>ACS Electronic Supporting Info</A></P>
Cytochrome $C_{550}$ is Related to Initiation of Sporulation in Bacillus subtilis
Shin Inji,Ryu Han-Bong,Yim Hyung-Soon,Kang Sa-Ouk The Microbiological Society of Korea 2005 The journal of microbiology Vol.43 No.3
The effect of cytochrome $c_{550}$ encoded by cccA in Bacillus subtilis during the event of sporulation was investigated. The sporulation of cccA-overexpressing mutant was significantly accelerated, while disruptant strain showed delayed sporulation in spite of the same growth rate. Activity of sporulation stage-0-specific enzyme, extracellular $\alpha-amylase$ of mutant strains was similar to that of the control strain, but cccA-overexpressing mutant exhibited higher activity of stage-II-specific alkaline phosphatase and stage-III-specific glucose dehydrogenase when compared to deletion mutant and control strain. Northern blot analysis also revealed that cccA-overexpressing mutant showed high level of spo0A transcripts, while the disruptant rarely expressed spo0A. These results suggested that although cytochrome $c_{550}$ is dispensable for growth and sporulation, expression of cccA may play an important role for initiation of sporulation through regulation of spo0A expression.
InJi Heo,Seulki Kim,Dong Young Hwang,Ji Won Jang,Young Seo Hong,Kui-Jae Lee,Tae Young Shin 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.10
Cryptotympana atrata belongs to the family Cicadidae, has long been recognized as a damaging plant-sucking pest, and is distributed in East Asian countries. In addition, their cries cause direct harm to us through noise pollution and also reported twig damage in the forest environments. In this study, we isolated strains of the entomopathogenic fungus Metarhizium that occurred from C. atrata collected this year. Here, we provide the morphological character and molecular phylogenetic relationship of this species. This is the first record of the entomopathogenic fungus Metarhizium viridulum isolated from C. atrata in Korea and provides a candidate strain with potential use for biological agents.
유한봉,Inji Shin,Hyung-Soon Yim,Sa-Ouk Kang 한국미생물학회 2006 The journal of microbiology Vol.44 No.2
In this study, we have attempted to characterize the functions of YlaC and YlaD encoded by ylaC and ylaD genes in Bacillus subtilis. The GUS reporter gene, driven by the yla operon promoter, was expressed primarily during the late exponential and early stationary phase, and its expression increased as the result of hydrogen peroxide treatment. Northern and Western blot analyses revealed that the level of ylaC transcripts and YlaC increased as the result of challenge with hydrogen peroxide. A YlaC-overexpressing strain evidenced hydrogen peroxide resistance and a three-fold higher peroxidase activity as compared with a deletion mutant. YlaC-overexpressing and YlaD-disrupted strains evidenced higher sporulation rates than were observed in the YlaC-disrupted and YlaD-overexpressing strains. Analyses of the results of native polyacrylamide gel electrophoresis of recombinant YlaC and YlaD indicated that interaction between YlaC and YlaD was regulated by the redox state of YlaD in vitro. Collectively, the results of this study appear to suggest that YlaC regulated by the YlaD redox state, contribute to oxidative stress resistance in B. subtilis.