관상동맥 질환에서 도플러 심초음파도로 측정한 확장기 좌심실기능의 지표와 혈역학적 소견과의 비교 검토

임헌관,기신영,유창범,최태명,김성구,권영주 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.1

Background : Left ventricular diastolic dysfunction is now recognized as a significant cause of cardiac symptoms even in patient with normal systolic ventricular function. During the last several years, the use of Doppler echocardiography for assessing left ventricular diastolic function has attracted increased attention. Doppler study have demonstrated that patients with ischemic heart disease have abnormal diastolic filling pattern suggesting impaired relaxation. Methods : This study was designed to correlate Doppler mitral flow velocity patterns and measuared variance with hemodynamic findings in 33 patients: 10 with myocardial infarction(group 1), 10 with angina pectoris(group 2) and 13 with no coronary disease(group 3). Results: 1. E/A ratio (0.87±0.24 m/sec versus 1.09±0.91 m/sec, P<0.05) was lower in group 1 than in group 3. 2. Significant correlation were demonstrated between pulmonary capillary wedge pressure (PCWP) and isovolumic relaxation time(IVRT) (r=-0.58; p<0.001), and E velocity (r=0.61; p<0.005). 3. IVRT was (61±30msec versus 92±35msec, p<0.05) shorter in patients with PCWP>10mmHg than in patients with PCWP≤10mmHg. Conclusion: Doppler mitral flow recordings have partially clinical potentiality in assessing left ventricalar diastolic function in patient with ischmic heart disease.

Enzymatic synthesis of glycosylated herboxidiene using glycosyltransferase from Bacillus licheniformis ATCC 14580

Chang Boem Lee,Sushila Maharjan,Ye Rin Kim,Kwangkyoung Liou,Jae Kyung Sohng 한국당과학회 2011 한국당과학회 학술대회 Vol.2011 No.1

Polyketides are a large class of natural products that include clinically important antibiotics, antitumor compounds, immunosuppressants, pigments and herbicides. Herboxidiene is a polyketide natural product that was found to control several important biannual weeds at relatively low application rates (<250 g/hectare) without damaging wheat. Early study indicated that the epoxide and the C-18 hydroxyl groups are important for the activity of herboxidiene. While Sakai et al., 2002 reported that herboxidiene showed antitumor activity against human tumor cell lines, Koguchi et al., 1997 reported that herboxidiene up-regulated the gene expression of low density lipoprotein receptors. The interesting biological profile of this natural product has prompted us to produce its glycosylated analogues so as to alter its activity. It is well known that bioactive natural products are often complexed with sugar moieties which are essential for activity of the compound. In this study, we carried out in vitro experiments to generate glucosyl-herboxidiene and galactosyl-herboxidiene.

라즈베리파이 기반의 홈로봇용 3D 물체 인식 시스템

김범창(Boem Chang Kim),김도현(Do-Hyun Kim),안현식(Hyunsik Ahn) 대한전자공학회 2016 대한전자공학회 학술대회 Vol.2016 No.11

안구건조증에서 정량적 눈물액 삼투압 측정을 통한 레스타시스Ⓡ의 치료 효과

장인범(In Boem Chang),김태진(Tai Jin Kim) 대한검안학회 2012 Annals of optometry and contact lens Vol.11 No.1

목적: 건성안에서 눈물액 삼투압 측정하여 레스타시스Ⓡ (Allergan Inc., Irvine, CA, USA)의 치료효과를 알아보았다. 대상과 방법: 건성안을 진단받은 환자 50명, 100안을 대상으로 레스타시스Ⓡ와 인공누액을 사용하며, LASEK 수술을 받은 군 22명 44안과 안과적 수술을 받지 않은 군 28명 56안에 대해 전향적 실험연구를 하였다. 설문조사, 쉬르머 검사, 눈물막파괴검사 및 눈물액 삼투압 검사는 치료 전과 치료 후 1, 2, 3개월 및 6개월에 시행되었다. 결과: 설문조사에서 안구 증상은 두 군 모두 레스타시스Ⓡ 점안 후 1개월째부터 호전되기 시작하였고, 쉬르머 검사, 눈물막파괴검사 및 눈물액 삼투압도 유의하게 개선되었다(p<0.05). 시기별 평균 삼투압변화는 치료시간과 유의한 상관성을 보였다(p<0.05). 두 그룹 모두에서 설문조사와 눈물액 삼투압수치는 감소했으며, 쉬르머 검사와 눈물막파괴시간 수치는 증가하였다. 두 그룹의 평균 삼투압은 레스타시스Ⓡ 점안 후 1, 2개월 및 6개월에서 유의한 차이를 보였다. 결론: 눈물액 삼투압 측정법은 건성안의 진단 및 치료효과 평가에 객관적이고 재현성 높은 검사로서 사용될 수 있을 것이다. Purpose: To evaluate the effect of 0.05% cyclosporine emulsion on tear osmolarity measured by TearLabTM(OccuLogix, San Diego, CA, USA) in dry eye syndrome. Methods: From patients diagnosed with dry eye syndrome, 0.05% cyclosporine emulsion and lubricant eye drop were administered to 100 eyes of 50 patients. The prospective study was enrolled with 44 eyes of 22 patients who had been performed with laser ephithelial keratomileusis (LASEK Group) previously and 56 eyes of 28 patients with no previous ocular surgical history (Non LASEK Group). From each patient, symptoms questionnaire based on ocular surface disease index (OSDI), Schirmer test with anesthesia, Tear film break up time (TBUT) and osmolarity were evaluated. The clinical parameters and symptoms were checked before and 1, 2, 3, and 6 months after treatment. Results: Ocular symptoms evaluated by OSDI symptoms questionnaire in LASEK and Non-LASEK group started to improve from 1 month after use of RestasisⓇ (Allergan Inc., Irvine, CA, USA) and Schirmer test, TBUT and osmolarity improved significantly (p<0.05). The changes of average values with time had a high correlation from 0.586 to 0.993 (p<0.05). Both OSDI and Tear osmolarity values decreased, and Schirmer test and TBUT values increased in each two group. The average values of tear osmolarity were statistically different at 1 month, 2 months and 6 months after administration of RestasisⓇ between two groups. Conclusions: The measurement of osmolarity of tear film can be used not only to diagnose the dry eye diease, but also to evaluate the therapeutic effect of RestasisⓇ as more objective and reproducible tests.

마우스에서 Cisplatin과 방사선조사로 유발된 구내염에 대한 재조합 표피성장인자의 효과

나재범(Jae Boem Na),김혜정(Hye Jung Kim),채규영(Gyu Young Chai),이상욱(Sang Wook Lee),이강규(Kang Kyoo Lee),장기철(Ki Churl Chang),최병옥(Byung Ock Choi),장홍석(Hong Seok Jang),정배권(Bea Keon Jeong),강기문(Ki Mun Kang) 대한방사선종양학회 2007 Radiation Oncology Journal Vol.25 No.4

목 적: 마우스 모델에서 cisplatin과 방사선조사로 구내염을 유발시킨 후 재조합 표피성장인자 (recombinant human epidermal growth factor, rhEGF)을 처치하여 그 효과를 알아보고자 하였다. 대상 및 방법: 마우스 24마리를 대상으로 정상대조군 8마리와 실험군은 각각 rhEGF 처치군과 미처치군으로 8마리씩 분류하였으며 실험군은 10 mg/kg의 cisplatin을 방사선조사 첫날 동시에 1회 복강 내 투여하였고, 방사선조사는 5일간 1회 5 Gy씩, 5일간 25 Gy를 조사하였다. RhEGF 처치군은 방사선조사 2일 전부터 2일간 1 mg/kg의 rhEGF를 피하주사하였으며 방사선조사 3일째부터 3일간 1 mg/kg를 피하주사하여 총 5 mg/kg의 rhEGF를 투여하였다. 마우스의 체중변화, 먹이섭취 및 조직학적 변화를 평가하였다. 결 과: 마우스 체중변화는 rhEGF 처치군이 실험 3일째부터 5일간 rhEGF 미처치군과 비교하여 통계적으로 유의한 체중의 차이를 관찰할 수 있었다. 먹이섭취는 실험군(rhEGF 처치군과 미처치군)에서 실험 5일째까지 감소하였다가 13일째부터 먹이섭취의 증가를 보여주었다. Cisplatin과 방사선조사 후 7일째의 조직학적 검사에서는 rhEGF 처치군 에서 마우스의 점막 표피층의 변성이 관찰되었으나 rhEGF 미처치군에서는 점막층의 염증 반응이 관찰되었다. 결 론: Cisplatin과 방사선 조사로 마우스에서 발생한 구내염에서 rhEGF를 투여한 경우 체중 변화와 먹이섭취의 유의한 개선을 관찰 하였으며 조직학적 검사에서 점막 손상의 회복을 확인하였다. 향후 임상적으로 rhEGF가 항암화학요법과 방사선치료로 인한 구내염을 줄일 수 있는 가능성을 확인하였다. Purpose: To study the effect of recombinant human epidermal growth factor (rhEGF) on oral mucositis induced by cisplatin and radiotherapy in a mouse model. Materials and Methods: Twenty-four ICR mice were divided into three groups— the normal control group, the no rhEGF group (treatment with cisplatin and radiation) and the rhEGF group (treatment with cisplatin, radiation and rhEGF). A model of mucositis induced by cisplatin and radiotherapy was established by injecting mice with cisplatin (10 mg/kg) on day 1 and with radiation exposure (5 Gy/day) to the head and neck on days 1∼5. rhEGF was administered subcutaneously on days -1 to 0 (1 mg/kg/day) and on days 3 to 5 (1 mg/kg/day). Evaluation included body weight, oral intake, and histology. Results: For the comparison of the change of body weight between the rhEGF group and the no rhEGF group, a statistically significant difference was observed in the rhEGF group for the 5 days after day 3 of the experiment. The rhEGF group and no rhEGF group had reduced food intake until day 5 of the experiment, and then the mice demonstrated increased food intake after day 13 of the of experiment. When the histological examination was conducted on day 7 after treatment with cisplatin and radiation, the rhEGF group showed a focal cellular reaction in the epidermal layer of the mucosa, while the no rhEGF group did not show inflammation of the oral mucosa. Conclusion: These findings suggest that rhEGF has a potential to reduce the oral mucositis burden in mice after treatment with cisplatin and radiation. The optimal dose, number and timing of the administration of rhEGF require further investigation.

Genome re-sequencing of rice core set for breeding: Practical comparative genomics through whole genome-wide allele mining of agronomic traits

Yong-Jin Park,Chang-Yong Lee,Tae-Sung Kim,Boem-Seok Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Genetic resources play a great role in crop breeding because of containing a broad array of useful genes. Currently, the harder are rice breeders trying to develop new rice cultivars with the improved traits, they are more often handicapped by the limited availability of germplasm resources. Thus, a desirable core or heuristic (HS) set of germplasm with maximum genetic diversity can be usefully exploited to breakthrough the present and future challenges of the rice breeding. As such we previously developed the rice HS sets of 166 diverse accessions out of a total 24,368 rice germplasms. Here, we report a large-scale analysis of the patterns of genome-wide genetic variations accumulated in the HS as well as Korean rice over the time. We characterized a total of about 11.8 millions of single nucleotide polymorphisms (SNPs) across the rice genome from resequencing a total of 295 rice genomes including 137 HS and 158 KB rice sets, with an average of approximately 10x depth and > 90% coverage. Using about 460,000 high-quality SNPs (HQSNPs), we specified the population structure, confirming our HS set covers all the rice sub-populations. We further traced the relative nucleotide variabilities of HQSNPs and found the level of the diversity was dynamically changing across the KB genome, which reveals the selection history of KB lines in the past and present. In addition, the results of our genome wide association study (GWAS) suggests that our HS can be also a good reservoir of valuable alleles, pinpointing those alleles underlying the important rice agronomical traits. Overall, the resequencing of our HS set re-illuminates the past, present of the germplasm utilization, which will support the Korean rice breeding in the future.

A Reliable Colorimetrical Assay for Measurement of rhGM-CSF Biological Activity

Chang-Koo Yun,Kyu-Boem Han,In-Seop Kim 한국생물공학회 2008 한국생물공학회 학술대회 Vol.2008 No.4

Effect of Heat Treatment and Glycosylation on in vitro Stability of rrhGM-CSF

Chang-Koo YUN,Kyu-Boem HAN,Youn-Je PARK 한국생물공학회 2012 한국생물공학회 학술대회 Vol.2012 No.4

Purification of Hyaluronidase from Bovine Testis and Comparison with Recombinant Hyaluronidase

Chang-Koo YUN,Kyu-Boem HAN,In-Seop KIM 한국생물공학회 2009 한국생물공학회 학술대회 Vol.2009 No.10

대학생의 기부행동과 기부인식

고희범, 김윤식, 장동명, 이희숙 충북대학교 생활과학연구소 2013 생활과학연구논총 Vol.17 No.2

The purpose of this study was to find charity act and awareness of university. Questionnaire survey was conducted from 11 July to 18 July 2011. And 300 university students who were attending universities in Cheongju area. Major findings as follows. First, most university students(86.1%) had experienced to donate money instead of their time or talents. And they donate to mostly religious institutes. These result implied that university students would be better time or talent donation rather than money, since they had no earned income yet. For this, it would be important that university headquarters or social institutes make opportunities to donate time or talents for university students. Second, it was found that why university students did not donate during university period. That is, first, university students did not believe charity institutes, second, university students have not been asked to donate, and university students thought that they had no money enough to donate. These results implied that charity institutions should have clear management system, and university students would be better time or talent donation rather than money again. Third, it was found that charity awareness of university students was more likely to be positive, and that the students who had donation experience showed more positive charity awareness than the counter part. This result implied to expecially parents that it is important socialization of children's donation behaviors and awareness.