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Mok, Il-Kyoon,Lee, Jae Kwon,Kim, Jeong Hwa,Pan, Cheol-Ho,Kim, Sang Min Elsevier 2018 Food chemistry Vol.258 No.-
<P><B>Abstract</B></P> <P>Our previous study reported the improved stability of fucoxanthin (FX) fortified in whole milk (WM) and skimmed milk (SM). In this study, <I>in vivo</I> and <I>in vitro</I> FX bioavailability were investigated using FX-fortified milk (FX-SM and FX-WM) and microalga <I>Phaeodactylum tricornutum</I> biomass (Pt-powder). Organ tissue accumulation of FX and its metabolites (FXOH: fucoxanthinol, AXA: amarouciaxanthin A) after repeated oral administration was in the following order: FX-SM > FX-WM > Pt-powder. <I>In vivo</I> pharmacokinetic study with a single oral administration also demonstrated that the absorption of FXOH and AXA was the highest for FX-SM. To reinforce the <I>in vivo</I> results, <I>in vitro</I>-simulated digestion and Caco-2 cell uptake assays were performed, which revealed that FX-SM showed the highest FX bioaccessibility (release from food matrices) and cellular uptake efficiency of FX and FXOH. In conclusion, skimmed milk was validated as an excellent food matrix for FX application in terms of stability and bioavailability.</P> <P><B>Highlights</B></P> <P> <UL> <LI> FX-SM showed the highest organ tissue accumulation and plasma absorption. </LI> <LI> Higher bioaccessibility and cellular uptake efficiency of FX-SM increased FX bioavailability. </LI> <LI> Higher protein and Ca<SUP>2+</SUP> ion and lower fat accounted for the higher bioaccessibility and cellular uptake efficiency of FX-SM. </LI> </UL> </P>
1H NMR spectroscopy를 이용한 수용액 상의 vitamin C와 EGCG의 보존성 분석
목일균 ( Il Kyoon Mok ),엄병헌 ( Byung Hun Um ) 충북대학교 과학교육연구소 2014 과학교육연구논총 Vol.29 No.1
The NMR (nuclear magnetic resonance) techniques were developed for the simultaneous quantitative analysis of L-ascorbic acid (L-ascorbic acid), EGCG (epigallocatechin gallate) and its degradation products in food storage conditions. Several NMR experimental conditions including water-suppression techniques, acquisition (relaxation delay, acquisition time, etc) and processing methods were optimized for quantification of food components. Sampling method with micro NMR-tube was also developed for food components stability study with normal water (H2O) without using deuterium. The quantities of L-ascorbic acid, EGCG and its degradation products were measured by the quantitative NMR method and HPLC analysis method, respectively. The analysis results of the two methods were compared. The NMR method showed the structure of degradation products, which is not possible in HPLC methods. The NMR is quick and simple to use. In stability study of food storage, This modified NMR technique provides simultaneous and quantitative information with chemical structure of food components without time consuming separation procedure and expensive deuterium solvent.
Koo, Song Yi,Mok, Il-Kyoon,Pan, Cheol-Ho,Kim, Sang Min American Chemical Society 2016 Journal of agricultural and food chemistry Vol.64 No.49
<P>To facilitate the utilization of fucoxanthin (FX), a valuable marine carotenoid, in the food industry, FX-loaded casein nanoparticles (FX-CN) and chitosan-coated FX-CN (FX-CS-CN) were developed using the FX-enriched fraction from Phaeodactylum tricornutum. Two nanoscale particles (237 +/- 13 nm for FX-CN and 277 +/- 26 nm for FX-CN-CN) with spherical and smooth surfaces showed over 71% encapsulation efficiency and polydispersity index (PDI) value of 0.31-0.39 in water. Owing to the chitosan coating, FX-CS-CN showed a positive zeta potential (24.00 mV), whereas that of FX-CN was negative (-12.87 mV). In vitro simulated digestion demonstrated better FX bioaccessibility from the nanoparticles versus P. tricornutum powder (Pt-powder) and from FX-CN versus FX-CS-CN. However, in C57BL/6 mice, fucoxanthinol absorption to the blood circulation was two times higher for FX-CS-CN versus FX-CN, possibly due to increased retention or adsorption to mucin by the cationic biopolymer in the chitosan-coated particles. These results demonstrate that FX-CS-CN can enable the application of FX, with improved bioavailability and water dispersibility, in the food industry.</P>
Yu, Shin-Hye,Kwak, So-Hyung,Nguyen, Thi Thanh Hanh,Seo, Ye-Seul,Song, Chaeri,Mok, Il Kyoon,Kim, Doman Springer Netherlands 2018 Biotechnology letters Vol.40 No.2
<P><B>Objectives</B></P><P>To develop preventive canine oral health bio-materials consisting of probiotics and glucanase to reduce insoluble glucan and volatile sulfur compound formation.</P><P><B>Results</B></P><P>Co-cultivation of <I>Enterococcus faecium</I> T7 with <I>Streptococcus mutans</I> at inoculation ratio of 3:1 (v/v) resulted in 25% reduction in the growth of <I>Streptococcus mutans</I>. Amounts of soluble and insoluble glucans produced by <I>S. mutans</I> were decreased to 70 and 55%, respectively. Insoluble glucan was decreased from 0.6 µg/ml in <I>S. mutans</I> culture to 0.03 µg/ml in <I>S. mutans</I> co-cultivated with <I>E. faecium</I> T7 in the presence of <I>Lipomyces starkeyi</I> glucanase. Volatile sulfur compound, a main component of halitosis produced by <I>Fusobacteria nucleatum</I>, was decreased by co-cultivating <I>F. nucleatum</I> with <I>E. faecium.</I></P><P><B>Conclusion</B></P><P><I>E. faecium</I> and glucanase can be combined as potentially active ingredients of oral care products for pets by reducing plaque-forming bacteria growth and their by-products that cause cavity and periodontal disease.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (10.1007/s10529-017-2478-z) contains supplementary material, which is available to authorized users.</P>