저장용액의 온도에 따른 치주인대세포의 생존율

조재현,김성오,최형준,이제호,손흥규,최병재 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.1

외상성 손상 후 치아 탈구 시 치아를 저장하는 저장용액의 종류와 온도가 치주인대세포의 생존율에 어떠한 영향을 미치는지 연구하기 위하여 치주인대세포를 10% fetus bovine serum(FBD) 함유 α-minimal essential medium(α-MEM)에서 37℃ 5% CO_(2) 공기 혼합 배양기에서 배양하고 4.25, 37℃의 Hank's balanced sa1t solution(HBSS)과 α-MEM, 우유(S회사, P회사), tap water에 저장하고 60분이 지난 후 각 군에 대해 치주인대세포의 생존율을 측정하기 위하여 MTT assay를 시행하여 다음과 같은 결과를 얻었다. 1. 4℃ 저장용액의 치주인대세포의 생존율은 α-MEM과 P회사우유에서 가장 높았고 HBSS, S회사우유, tap water 순으로 낮았다. 2. 25℃ 저장용액의 치주인대세포의 생존율은 α-MEM에서 가장 높았고 P회사우유, HBSS, S회사우유, tap water 순으로 낮았다. 3. 37℃ 저장용액의 치주인대세포의 생존율은 α-MEM과 P회사우유, HDSS, S회사우유에서 높았고 tap water에서 가장 낮았다. 4. α-MEM의 치주인대세포의 생존율은 4℃에서 가장 높았고 25℃와 37℃ 순으로 낮았으며 HBSS에서는 4℃에서 높았고 25℃와 37℃에서 낮았다. 5. S회사와 P회사우유에서 치주인대세포의 생존율은 4℃와 25℃에서 높았고 37℃에서 낮았다. 이상의 결과를 종합해볼 때 외상으로 인해 치아가 탈구되었을 때 탈구된 치아의 저장용액으로 HBSS 용액이 추천되고 있으나 이 연구에서 4℃와 25℃ 우유에서 치주인대세포의 생존율이 높았으므로 사고 현장에서 쉽게 구할 수 있고 치주인대세포의 생활력 보존에도 유리한 낮은 온도의 우유에 탈구된 치아를 보존하는 방법도 좋을 것으로 생각된다. To compare the survival rate of periodontal ligament cells preserved in storage media with good availability at the time of an avulsion injury, periodontal ligament cells were incubated in α-MEM culture medium containing 10% FBS in condition of 37℃, 5% CO_(2). These cells were then cultured in HBSS, α-MEM, milk(S. co., P. co.) and tap water at the temperature of 4, 25, 37℃ each in 60 min. The groups were measured by MTT assay. The results were as follows : 1. Among the storage media at 4℃, α-MEM and P-milk had the highest preserving ability of periodontal ligament cells, while that of HBSS, S-milk and tap was low in order. 2. Among the storage media at 25℃, α-MEM had the highest preserving ability of periodontal ligament cells, while that of P-milk, HBSS, S-milk, tap water was low in order. 3. Among the storage media at 37℃, the preserving ability of periodontal ligament cells was very high in α-MEM, P-milk, HBSS and S-milk. it' s lowest in tap water. 4. The preserving ability of periodontal ligament cells in α-MEM was high at 4℃ and it’s low in order of 25℃, 37℃, but in HBSS was high at 4℃ and it's low at 25℃, 37℃. 5. The preserving ability of periodontal ligament cells in S-milk and P-milk was high at 4℃, 25℃ and it’s low at 37℃. In conclusion, HBSS is the storage medium of choice in an avulsion, but in this study it is preferable to choose milk at 4℃ for tooth since it is easy to get and affect cell viability.

스포츠 현장에서 사고 분쟁해결을 위한 법적 구조 및 그 효용성 분석

박형상,육조영,김우규,이재봉,유관호,이무연,박태민,이신언 한국스포츠리서치 2005 한국 스포츠 리서치 Vol.16 No.2

1. The purpose of this study is to reflect reality of sports to legal structure. This author classified types of sports related accidents as match accidents, school accidents, leisure and living sports accidents. Out of such accidents common factors such as 'the provider of virtue' and 'user' could be deduced and this author reviewed what kinds of obligations must be shouldered by these people respectively. In particular this author stressed the fact that disproportionate emphasis is placed on malfeasance in disposition of dispute over the sports accidents. Malfeasance is a method for seeking relief through money after the accident. However default in an obligation enables supplementary completion when it is not performed or imperfectly performed. Thus if object which must be performed is providing safety then it is possible to secure safety prior to accident. Furthermore at such time mistake of the debtor can not become a factor. For this reason this line of thinking has the following merits: (1) In comparison with constitution of malfeasance default of an obligation has long prescription. It seems that such fact was a motivation for saving obligation for safety consideration by making it fall under the principle of faith. (2) Default of an obligation is not an obligation for ordinary attention which is cause of malfeasance but it takes up issue with obligation for attention required for performance. Game organization, sponsor and spectators etc. shares certain sense of identity and are demanded to have manners. As method for evaluation of such relationship of faith it may be possible to impose a special kind of obligation for attention and it will be close to constitution of default of an obligation. (3) Imposing obligation for provision clarifies that such responsibility belongs to game organizations promoting sports to which anyone can participates or to national or public organizations, sponsor, schools which induced such sports as a part of its educational programs, installer who made people use it as leisure facility or person in charge for its management. (4) It is also applicable to umpires, players or spectators who use field of sports. It is possible to reflect reality whereby these people specifically relate themselves to sports. In consequence it will be possible to make people feel the necessity of expanding the principals of sports from 'doers' to 'watchers.' (5) The obligation of provision is an obligation under the principle of faith therefore mutual negotiation may be expected to clarify such fact. It may be also evaluated by effectuation of users' self-determining right. 2. If the above mentioned structure is provided then, although it is limited to sports, students who are subjects of instruction, seminar attendants, and furthermore players who finished games may be obligated to bear responsibility for safety consideration under the principle of faith. Accordingly level of relief for considerable number of victims may be increased as cases of treating even borrowed materials as default of obligation being expanded. 3. As scope of responsibility becomes more clearer it will be possible to prevent disputes while securing more safety as rules and regulations become more clearly enacted or new contract is signed or existing contract is revised.

무선온도계측에 의한 현장타설 콘크리트의 양생온도관리

김형래,조호규 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

The purpose of this study is on the utilization of curing management method of concrete by wireless data aquisition system. Applied system is consisted of a data-logger to measure the concrete temperature and a wireless-modem to communicate with the data-logger. In this study, the process to monitor concrete temperature and to take an appropriate measure by the system was investigated. As the results, through the measurement of concrete temperature by wireless aquisition system, almost same results with the case of existing auto-recording system were acquired. The wireless aquisition system could make sensing the steep variation of temperature and proper measurements for additional curing possible.

침엽수로부터 발생되는 방향성 테르펜의 배출속도 비교 연구

김조천,홍지형,강창희,선우영,김기준,임준호 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.2

In order to investigate the composition ratio and the emission rate of monoterpenes emitted from coniferous trees during summer, the enclosure chamber experiments were carried out for two tree species (Pinus densiflora and Pinus rigida). The major monoterpenes from the pine trees were α-pinene, β-pinene, myrcene and β-phellan-drene. There was no difference of the composition ratio between two sites for P. densiflora, but the difference was observed for P.rigida, Clearly, the ratio of β-pinene (26.1%) for P.rigida was higher than that of β-phellandrene (12%) at the Gumsung mountain site, but the ratio of β-phellandrene (22.2%) was higher than that of β-pinene (9.9%) at the Chiri mountain site. Particularly, the composition ratio of β-phellandrene was higher than that of β-pinene for P.densiflora, and the opposite trend was found for P.rigida at the Gumsung mountain site. The characteristics of the composition ratio between two species were similar at the Chiri mountain site. As a result of the emission rate measurements, it was observed that both sites did not show any significant β value (slope between emission rate and temperature) and ERs (Standard Emission rate at 30℃) difference. However, the β value and ERs at the Chiri mountain site were slightly greater than those of Gumsung mountain site for both pine species. P.densiflora(1.703 and 1.971㎍C/gdw/hr) showed the higher monoterpene ERs than those of P.rigida (0.572 and 0.698 ㎍C/gdw/hr) at both sites.

담양신설 IC의 교통소음도 조사연구

최형일,강영주,채희정,김연풍,조도호,설동화,정숙경 조선대학교 환경공해연구소 2007 環境公害硏究 Vol.20 No.1

After the vehicle has become a large formation with high-speed, loud noise from an express highway is increasing, and the number of large-scale apartment complexes, which are recently being constructed in the metropolitan area nearby the express highway and IC, is increasing gradually. Compared with a main road, the traffic volume and the speed of a vehicle between IC and the ramp way [turn-off] are smaller. If the ramp way is rightly connected with the downtown area, however, the traffic-noise that comes about in surrounding areas will be in conjunction with the traffic-noise from the main road of express highway and that of the ramp way. This research has selected a place that hasn't had a road or a commercial quarter nearby the main-road of IC and ramp way. Then we have studied the traffic-noise status under minimizing influence of its outer-noise, which occurs in IC. 1. The traffic volume change of a small and full-sized car with time has been kept on regularity in 2004 and 2005. As the traffic volume of a full-sized car has diminished remarkably, it has been maintaining through 6:00a.m.-10:00 a.m. As it has decreased since lO:OO a.m.. we have also observed the traffic proportion going up. 2. Monthly traffic volume of small cars was higher in May, August and October than other months. We have observed that the high traffic volume during these months is due to the following: May-The Damyang festival, August-a high-demand season, October-viewing scarlet maple leaves. Monthly traffic volume of full-sized cars was generally higher in August, but it has decreased during 2006. This was due to the many construction trucks that were working on the extension of the 88 Olympic expressway. Therefore, there was more traffic volume in 2006 compared to 2004 and 2005. 3. In this study, the noise figures have been less than 55 dB(A) in 18 spots. There were 14 spots from 55dB(A) to 58dB(A). 58-61dB(A) : 20, 61-64dB(A) : 17, 64-67dB(A) : 18. There were 8 spots we observed at the highest level from 67dB(A) to 70dB(A). Those were the nearby spots separating the main road and Damyang IC. 4. Traffic-noise at the boundary between IC and the main road of an expressway was higher than that of the same distance in other places. 5. We have observed that traffic-noise is low in places with mountains that serve the role of soundproofing walls. Thus, the fundamental sound hadn't affected it.

월출산국립공원 수질특성에 관한 연구

최형일,김난희,강영주,채희정,김연풍,조도호,설동화 조선대학교 환경공해연구소 2007 環境公害硏究 Vol.20 No.1

Because mountain streams have narrow widths and steep slopes compared to streams and lakes, hours of immobility are considerably short, and the quality of mountain streams has been greatly influenced by rainfall, land-use, components of the streambed and types of forests. During the summer when heavy rainfall occurs, eroded earth and sand flow into mountain streams and cause temporarily high turbidity. It is also the cause of eutrophication, which is frequently found in downstream. As evaluation standards for the quality of mountain streams were not established in Korea, this quality was evaluated by examining some items based on quality standards for drinkable water and research on the influences of pollution in mountain streams, which showed that these were seldom in downstream water systems. Mountain streams within Wolchulsan National Park flow into a reservoir and when characteristics of mountain streams from forests and rainfall that flow into mountain streams through forest ecosystems were studied, results of measuring its changes are as follows. Monthly changes of pH were low between April and August and higher starting October, and the EC was measured higher in the winter and the spring and tended to be low starting April. The EC on the 19th of May and the 23rd of October and the concentration of floating materials and COD_(Mn) were observed to be higher due to the influence of rainfall on the 19th of May and the 22nd of October, and it was judged that the washing out of dried deposits attached to the water pipe and the leaching of woods were influenced by rainfall. It is considered that real-time monitoring of water quality for a long time and water quality conditions during and after rainfall should be examined for ensuring basic data of water quality and evaluating characteristics and seasonableness of mountain streams, which may be greatly changed by artificial influence in the future.

Sports에서 Rule의 본성과 성격 분석

박형상,육조영,유관호,박태민 한국스포츠리서치 2005 한국 스포츠 리서치 Vol.16 No.2

This study begins with the following questions. Why should athletes keep rules of sport? What occurs in sport when cheaters break the rules? In relation to these questions, which explore basis or foundation of the rules. there are some precedent theories. Those are 1) rule absolutism of game formalism. 2) contextual contractualism, 3) externalism. Through my research on 1) and 2), a very important issue. namely, the ethos of the game rose. That is, an internal purpose of the game achieved as a result of realization of the game. and at the same time the basis of the rules. Furthermore, with regard to 3), it is not able to point out the basis of the rules because of denying the existence of this ethos: "internal purpose of sport". All things considered, the ethos of game is to make a decision of victory or defeat. Therefore, a norm "Keel) rules" itself is not always an absolute command, a categorical imperative, but a relative one. a hypothetical imperative. Consequently, all cheating, rule breaking, doesn't act on the game destructively. The rules of sport is fundamentally restricted by the ethos of sport under all circumstances. In other words. only this ethos: "the spirit of the sport", forms the basis or foundation of the rules of sport.

도로교통소음의 수평·수직적 전달 및 감쇠 특성

최형일,채희정,김연풍,조도호,설동화,정숙경,김정애 조선대학교 생산기술연구소 2007 生産技術硏究 Vol.29 No.1

This study has been conducted to achieve the following objectives: First, in order to understand the horizontal propagation and attenuation characteristics for the road traffic noise, we selected actively developed areas within 100 meters away from the road to measure the level of traffic noise near a road. To measure the noise near a railroad, we selected areas within 100 meters away from the road. In this way, we analyzed the horizontal propagation and attenuation characteristics for the traffic noise occurring in diversified areas. Second, in order to understand the vertical propagation and attenuation characteristics for the road traffic noise, we measured and analyzed the distributional characteristics of vertical sound pressure levels on each floor of multi-storied apartment buildings according to changes of traffic load and types, and the existence or nonexistence of soundproof walls. For the case of the road traffic noise, we also selected we measured and analyzed the different noise levels on each floor of multi-storied apartment buildings from the soundproof wall. The results of Horizontal propagation and attenuation characteristics for the road traffic noise are as follows: In cases of the soundproof wall + raising ground, raising ground, cutting land, and flat land as distance increases, the sound pressure level steadily decreases. The soundproof wall + raising ground has an especially high distance decrease compared to the other areas, and the sound pressure level for the raising ground is higher than that of the cutting land with a measurement of 10.7~12.1dB(A). Vertical propagation and attenuation characteristics for the road traffic noise are as follows: The amount of sound pressure level decrease is 12.6- 14.5dB(A) for first floor when measuring the vertical sound pressure levels at soundproof wall, which indicates a fairly large decrease. At fifth and sixth floor, the amount of decrease is 2.3dB(A), which indicates a small decrease.

심첨부 비후 심근증에서 심근 내 미세 관상동맥의 혈류 예비력 평가 : 경흉부 도플러 심초음파를 이용한 연구 Study Using Transthoracic Doppler Echocardiography

윤호중,박철수,신재원,임상현,조은주,정해억,전희경,오용석,정욱성,김재형,최규보,홍순조 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.3

유산균(L.lactis CBT-8)및 발효 추출물의 Helicobacter pylori 억제 능력

진춘조,박형석,이혜운,김성렬,강동구,이준상,이재동,왕준호 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

Helicobacter pylori infection was the cause of chronic gastritis, duodenal ulcer and gastric MALT lymphoma. The eradication rates of H. pylori using antibiotics are around 80%. Lactobacilli have been demonstrated to have in vivo and in vitro inhibitory effects on H. pylori infection. We aimed to evaluate the inhibitory effect of L. lactis CBT-8 and its extract on H. pylori in human stomach. Thirty-five H. pylori-infected volunteers(30-49 yr)were randomized into two groups which were treated with L. lactis CBT-8 and its extract(Group A n=17) or milk containing L. lactis CBT-8(Group B, n=18). They underwent 13C-urea breath tests before and after treatment. The DOB30 of group A(n=14) was decreased 41.0% after treatment(26.0±4.9 before, 15.3±2.1 after treatment)(p<0.05). the DOB30 of group B(n=13) was decreased 22.3% after treatment(22.0±2.2 before, 17.1±1.7 after treatment)(p<0.05). In conclusion, L. lactis CBT-8 was effective in suppressing H. pylori infection in human stomach.