Error assessment of climate variables for FAO-56 reference evapotranspiration

Kwon, Hyojung,Choi, Minha Springer-Verlag 2011 Meteorology and atmospheric physics Vol.112 No.1

Seasonality of Net Ecosystem Carbon Exchang in Two Major Plant Functional Types in Korea

Hyojung Kwon,Tae-Yoon Park,Jinkyu Hong,Jong-Hwan Lim,Joon Kim 한국기상학회 2009 Asia-Pacific Journal of Atmospheric Sciences Vol.45 No.2

The processes in terrestrial ecosystems forma dynamic boundary interface within the earth climate system. The ecosystems in Asia, which are characterized by their diverse plant functional types, are currently undergoing the most rapid land­use changes, likely changing the feedback mechanismin land­atmosphere interaction. In this paper, we report seasonal variability of carbon balance over twomajor plant functional types in Korea: (1) a deciduous forest in a complex terrain (GDK site) and (2) a farmland with heterogeneous mosaic patches in a relatively flat terrain (HFK site). Our analysis is based on the year 2006, which was considered near normal in the sense of climate change and was of high quality data. Annually integrated values of gross primary productivity (GPP), ecosystem respiration (RE), and net ecosystem CO₂ exchange (NEE) at the GDK site were 835, 746, and ­87 g C m<SUP>­2</SUP> whereas GPP, RE and NEE at the HFK site were 1003, 993, and ­10 g C m<SUP>­2</SUP>, respectively. Despite its greater GPP, the HFK site was a weaker carbon sink than the GDK site because the REwas as large as the GPP. The annual patterns of each carbon budget component had amid­season depression, showing two distinctive peaks with different timing and magnitude for the two sites. Such a different bimodal seasonality was associated with different timing and intensity of the disturbances such as summermonsoon and the subsequent passing of typhoons later in the season. As far as we know, this is the first observation of the occurrence ofmid­season depression of NEE, which is likely due to the reduced GPP rather than the enhanced RE. Considering the current changes in hydrological cycles in the Asian region, further investigations along with fluxmeasurements in the rain are needed to clarify the causal relationship of ecosystem properties (e.g., NEE) with disturbances (e.g., summer monsoon, typhoons) and management (e.g., crop rotation).

Molecular cloning and characterization of a lysozyme cDNA from the mole cricket Gryllotalpa orientalis (Orthoptera: Gryllotalpidae)

Kwon, Hyojung,Bang, Kyeongrin,Lee, Minsup,Cho, Saeyoull Springer-Verlag 2014 MOLECULAR BIOLOGY REPORTS Vol.41 No.9

Granulocytes are Master Player of Cellular Immune Responses in Larva of Protaetia brevitarsis seulensis (Kolbe)

Hyojung Kwon,Kyeongrin Bang,Jiae Lee,Sejung Hwang,Saeyoull Cho 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

Hemocytes are key players in the immune response against pathogens in insects. However, the hemocyte types and their functions in the white-spotted flower chafers, Protaetia brevitarsis seulensis (Kolbe), are not known. In this study, we used various microscopes, molecular probes, and flow cytometric analyses to characterize the hemocytes in Protaetia brevitarsis seulensis. we identified the professional phagocytes, granulocytes (GRs), which mediate encapsulation and phagocytosis of pathogens. The GRs were immunologically or morphologically activated and phagocytosed potentially hazardous substances in vivo.

Seasonal Variability of Net Ecosystem Carbon Exchange in Two Major Ecosystems in Korea

Hyojung Kwon,Jinkyu Hong,Joon Kim 한국기상학회 2008 한국기상학회 학술대회 논문집 Vol.2008 No.-

Isolation and Characterization of Six Microorganisms from the Digestive Tract of the Cricket Gryllus bimaculatus

Kisang Kwon(권기상),Eun Ryeong Lee(이은령),Bo-Kyung Yoo(유보경),Young Hwa Ko(고영화),Hyojung Shin(신효정),Ji-Young Choi(최지영),O-Yu Kwon(권오유) 한국생명과학회 2017 생명과학회지 Vol.27 No.9

우리는 처음으로 쌍별귀뚜라미 소화기관에서 6종류의 미생물을 분리하고 특성을 규명하였다. 이들은 16S rDNA을 기준으로 분류한 결과 4종류(Staphylococcus, Bacillus, Citrobacter, Proteus)에 속하였다. 분리된 6종류의 미생물은 공통적으로 ampicillin에 저항성을 보이지만 kanamycin 저항성은 보이지 않았다. 이들을 Gram염색하여 미생물의 형태적 특징을 확인하였다. Gram-positive한 rod-shaped GL2와 round-shaped GL4는 다른 분리 균 보다 많은 양의 세포외분비물을 만들었다, 이들을 MALDI-TOF-MS spectral analysis결과 87-kDa collagenase, 56-kDa & 200-kDa hypothetical protein이였다. 새롭게 분리된 6종류의 미생물은 귀뚜라미의 생리에 미치는 영향과 이들의 생물공학적 혹은 해충 방제에 이용될 수 있는 연구가 기대된다. We describe the isolation and characterization of six different intestinal microorganisms from the digestive tract of the cricket Gryllus bimaculatus. Based on 16S rRNA gene sequences, we obtained six isolates belonging to four different genera: Staphylococcus, Bacillus, Citrobacter, and Proteus. All the isolates were resistant to ampicillin. Ampicillin is an irreversible inhibitor of the enzymeetranspeptidase, which is needed to make bacterial cell walls. None of the isolates were resistant to kanamycin, which binds to the 30S subunit of the bacterial ribosome and then inhibits total protein synthesis. Gram staining was conducted, in addition to morphological classification under a microscope. Four grampositive isolates and two gram-negative isolates were detected. The gram-positive isolates were GL1 (round shaped, 2 am in diameter), GL2 (rod shaped, 2.5 μm in length), GL3 (rod shaped, 2 μm in length), and GL4 (round shaped, 1.5 μm in diameter). The gram-negative isolates were GL5 (rod shaped, 2 μm in length) and GL6 (rod-shaped, 2.5 μm in length). Notably, two of the isolates, GL2 and GL4, secreted specific extracellular proteins. These were determined by MALDI-TOF-MS spectral analysis to be a 87 kDa collagenase, 56 kDa hypothetical protein, and 200 kDa hypothetical protein. The six isolates in this study could be used for various biotechnological applications and pest management, both in the field and in greenhouse systems. In addition, it would be interesting to determine the relationship between these isolates and their host.

누에 short neuropeptide F receptor (BsNPF-R)의 cDNA cloning

신효정(Hyojung Shin),권기상(Kisang Kwon),홍선미(Sun Mee Hong),김홍근(Hong Geun Kim),박관호(Kwan-Ho Park),최지영(Ji-Young Choi),김승환(Seung-Whan Kim),유권(Kweon Yu),권오유(O-Yu Kwon) 한국생명과학회 2016 생명과학회지 Vol.26 No.6

누에(Bombyx mori)에서 short neuropeptide F (sNPF) receptor를 encoding하고 있는 cDNA를 cloning하여서 BsNPF-R라고 이름을 붙였다. BsNPF-R는 이미 보고된 sNPF-R들과 아미노산 수준에서 사람(36%), 쥐(34%), 제브라피쉬(35%), 초파리(51%)와 상동성을 보였다. BsNPF-R는 계산적으로 분자량이 42,731 Da이고 원형질막을 관통하는 단백질이다. BsNPF-R 유전자발현은 중장, 후견사선, 말피기관, 정소에서 강한 반면 지방체, 혈세포, 난소에서 약하게 발현하였다. 그리고 합성된 sNPF에 의해서도 BsNPF-R의 유전자발현이 조절되었다. It has already been reported that short neuropeptide F (sNPF) stimulates feeding behaviors in a wide variety of insect species. In the present study, we cloned cDNA, encoding a sNPF receptor homologue from a silkworm, Bombyx mori, named BsNPF-R. The amino acid sequence of BsNPF-R was compared with those of sNPF-R thus far reported, which is shared with humans (36%), mice (34%), zebrafish (35%), and fruit flies (51%), respectively. A BsNPF-R protein"s mass was theoretically estimated to be 42,731 Da and it is a putative plasma membrane-penetrating protein. The mRNA expression of BsNPF-R was tested; the results showed that a strong expression was detected at the midgut, post-silk gland, Malpighian, and testis; however, a weak expression was at the fat body, hemocyte, and ovary. In addition, the synthesized sNPF of a silkworm regulated the BsNPF-R mRNA expression through the cell-based functional analysis.

Molecular Cloning and Characterization of a Lysozyme cDNA From the Mole Cricket Gryllotalpa orientalis (Orthoptera: Gryllotalpidae)

Kyeongrin Bang,Hyojung Kwon,Minsup Lee,Jiae Lee,Sejung Hwang,Saeyoull Cho 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

A full-length lysozyme cDNA from Gryllotalpa orientalis was cloned and sequenced. The deduced amino acid sequence of the lysozyme protein was 143 amino acids in length, with a calculated molecular mass of 15.84 kDa and an isoelectric point of 4.74. Sequence motifs, together with alignment and phylogenetic results, confirmed that G. orientalis lysozyme belongs to the C (chicken)-type lysozyme family of proteins. The protein sequence of lysozyme from G. orientalis showed high identity to that of Drosophila melanogaster (51.7%); however, in contrast to D. melanogaster lysozyme, G. orientalis lysozyme was immune inducible and expressed in a wide range of tissues. Expression of G. orientalis lysozyme mRNA was highest at 8 h post-infection and subsequently decreased with time after bacterial infection. We also expressed G. orientalis lysozyme protein in vitro using the pET expression system. Compared with the negative control, over-expressed G. orientalis lysozyme showed antimicrobial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Bacillus subtilis by radial diffusion assay, with MIC values of 30.3 μM and 7.55 μM respectively. These results indicate that G. orientalis lysozyme may have stronger antimicrobial activity than other lysozymes against a broad range of microorganisms.

Efficacy of the online Mindful Self-Compassion for Healthcare Communities program for surgical trainees: a prospective pilot study

Hyojung Shin,Heung-Kwon Oh,Yungsook Song,Yang Sun Kim,Bo Yeon Hur,Duck-Woo Kim,Sung-Bum Kang 대한외과학회 2023 Annals of Surgical Treatment and Research(ASRT) Vol.104 No.4

Purpose: The efficacy of the Mindful Self-Compassion (MSC) for Healthcare Communities program has not been verified. This study aims to evaluate the feasibility and efficacy of the online MSC for Healthcare Communities program on burnout, stress-related health, and resilience among surgical trainees. Methods: A single-arm pilot study was conducted at a tertiary referral academic hospital in Korea. Surgical trainees were recruited through flyer postings; therefore, a volunteer sample was used. Thus, 15 participants participated, among whom 9 were women and 11 were doctor-residents. The Self-Compassion for Healthcare Communities (SCHC) program was conducted from September to October 2021 via weekly online meetings (1 hour) for 6 weeks. The efficacy of the program was evaluated using validated scales for burnout, stress, anxiety, depression, self-compassion, and resilience before and after the intervention and 1 month later. Results: The results showed significantly reduced burnout, anxiety, and stress scores. After the program, high emotional exhaustion and depersonalization rates decreased, and personal accomplishment increased. Eight participants showed reduced anxiety postintervention, and 9 showed reduced stress. Improvements were observed between pre- and postintervention in resilience, life satisfaction, and common humanity. Changes in self-compassion predicted higher gains in resilience and greater reductions in burnout and stress. Conclusion: The SCHC is a feasible and effective program to improve resilience, self-compassion, and life satisfaction and reduce stress, anxiety, depression, and burnout in surgical trainees. This study highlights the need to include specific mental health programs in surgical training to improve trainees’ well-being.

Corn Gluten Hydrolysate Affects the Time-Course of Metabolic Changes Through Appetite Control in High-Fat Diet-Induced Obese Rats

Lee, Hyojung,Lee, Hyo Jin,Kim, Ji Yeon,Kwon, Oran Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.12

This study first investigated the effects of corn gluten hydrolysate (CGH) (1.5 g/day) administration for 7 days on appetite-responsive genes in lean Sprague-Dawley (SD) rats. In a second set of experiments, the metabolic changes occurring at multiple time points over 8 weeks in response to CGH (35.33% wt/wt) were observed in high-fat (HF, 60% of energy as fat) diet-fed SD rats. In lean rats, the hypothalamus neuropeptide-Y and proopiomelanocortin mRNA levels of the CGH group were significantly changed in response to CGH administration. In the second part of the study, CGH treatment was found to reduce body weight and perirenal and epididymal fat weight. CGH also prevented an increase in food intake at 2 weeks and lowered plasma leptin and insulin levels in comparison with the HF group. This reduction in the plasma and hepatic lipid levels was followed by improved insulin resistance, and the beneficial metabolic effects of CGH were also partly related to increases in plasma adiponectin levels. The Homeostasis Model of Assessment - Insulin Resistance (HOMA-IR), an index of insulin resistance, was markedly improved in the HF-CGH group compared with the HF group at 6 weeks. According to the microarray results, adipose tissue mRNA expression related to G-protein coupled receptor protein signaling pathway and sensory perception was significantly improved after 8 weeks of CGH administration. In conclusion, the present findings suggest that dietary CGH may be effective for improving hyperglycemia, dyslipidemia and insulin resistance in diet-induced obese rats as well as appetite control in lean rats.