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Shim, Hyeeun,Shim, Eunsook,Lee, Hansoo,Hahn, Janghee,Kang, Dongmin,Lee, Yun-Sil,Jeoung, Dooil Korean Society for Molecular Biology 2006 Molecules and cells Vol.21 No.3
<P>We previously identified a novel cancer/testis antigen gene CAGE by screening cDNA expression libraries of human testis and gastric cancer cell lines with sera of gastric cancer patients. CAGE is expressed in many cancers and cancer cell lines, but not in normal tissues apart from the testis. In the present study, we investigated its role in the motility of cells of two human cancer cell lines: HeLa and the human hepatic cancer cell line, SNU387. Induction of CAGE by tetracycline or transient transfection enhanced the migration and invasiveness of HeLa cells, but not the adhesiveness of either cell line. Overexpression of CAGE led to activation of ERK and p38 MAPK but not Akt, and inhibition of ERK by PD98059 or p38 MAPK by SB203580 counteracted the CAGE-promoted increase in motility in both cell lines. Overexpression of CAGE also resulted in a reduction of ROS and an increase of ROS scavenging, associated with induction of catalase activity. Inhibition of ERK and p38 MAPK increased ROS levels in cells transfected with CAGE, suggesting that ROS reduce the motility of both cell lines. Inhibition of ERK and p38 MAPK reduced the induction of catalase activity resulting from overexpression of CAGE, and inhibition of catalase reduced CAGE-promoted motility. We conclude that CAGE enhances the motility of cancer cells by activating ERK and p38 MAPK, inducing catalase activity, and reducing ROS levels.</P>
Li, Zhengzheng,Shim, Hyeeun,Cho, Myeong Ok,Cho, Ik Sung,Lee, Jin Hyun,Kang, Sun-Woong,Kwon, Bosun,Huh, Kang Moo Elsevier 2018 Carbohydrate Polymers Vol.184 No.-
<P><B>Abstract</B></P> <P>The use of injectable hydrogel formulations have been suggested as a promising strategy for the treatment of degenerative disc disease to both restore the biomechanical function and reduce low back pain. In this work, a new thermo-sensitive injectable hydrogels with tunable thermo-sensitivity and enhanced stability were developed with <I>N</I>-hexanoylation of glycol chitosan (GC) for treatment of degenerative disc disease, and their physico-chemical and biological properties were evaluated. The sol-gel transition temperature of the hydrogels was controlled in a range of 23–56 °С, depending on the degree of hexanoylation and the polymer concentration. <I>In vitro</I> and <I>in vivo</I> tests showed no cytotoxicity and no adverse effects in a rat model. The hydrogel filling of the defective IVD site in an ex vivo porcine model maintained its stability for longer than 28 days. These results suggest that the hydrogel can be used as an alternative material for treatment of disc herniation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A series of thermo-sensitive <I>N</I>-hexanoyl glycol chitosans are synthesized by <I>N</I>-hexanoylation of glycol chitosan. </LI> <LI> The hydrogel system is optimized for thermo-sensitivity, gel stability, and injectability. </LI> <LI> The hydrogels can be useful as an alternative material for treatment of disc disease. </LI> </UL> </P>
( Myung-gyun Kang ),( Daeui Park ),( Hyoung-yun Han ),( Hyeeun Shim ),( Yoonjung Hong ),( Jiyeon Moon ),( Seokjoo Yoon ),( Bosun Kwon ) 대한피부과학회 2019 Annals of Dermatology Vol.31 No.5
Background: Androgenic alopecia (AGA) is the most common type of hair loss. It is likely inherited genetically and is promoted by dihydrotestosterone. 5α-reductase has been proven a good target through finasteride use. However, the pathogenesis of AGA cannot be fully explained based only on dihydrotestosterone levels. Objective: To identify similar hairloss inhibition activity of RE-ORGA with mode of action other than finasteride. Methods: We prepared RE-ORGA from Korean herb mixtures. We performed MTT assays for cytotoxicity, Cell Counting Kit-8 assays for cell proliferation, and western blot to identify expression levels of 5α-reductase and Bax. RNA-sequencing was performed for the expression patterns of genes in dihydrotestosterone-activated pathways. Anti-inflammatory activity was also assessed by the expression levels of tumor necrosis factor-alpha (TNF-α) and interleukin 6. Results: REORGA could promote the proliferation of human dermal papilla cells and showed low cytotoxicity. It also inhibited the expression of 5α-reductases and Bax in the cells. RNA-sequencing results verified that the mRNA expressions of SRD5A1, Bax, transforming growth factor-beta 1 (TGF-β1), and TGF-β1 induced transcript 1 (TGFβ1I1) were decreased, whereas expression of protein tyrosine kinase 2 beta (PTK2β) was more elevated. REORGA also showed anti-inflammatory activity through decreased mRNA levels of TNF-α. Conclusion: Transcriptionally, up-regulation of PTK2β and concomitant down-regulation of TGFβ1I1 imply that RE-ORGA can modulate androgen receptor sensitivity, decreasing the expression of 5α-reductase type II and Bax together with TGF- β1 transcripts; RE-ORGA also showed partial anti-inflammatory activity. Overall, RE-ORGA is expected to alleviate hair loss by regulating 5α-reductase activity and the receptor’s androgen sensitivity. (Ann Dermatol 31(5) 530∼537, 2019)