Determination of Acyl-lysophosphatidic Acid in Plasma by Tandem Mass Spectrometry

Yoon, Hye-Ran 덕성여자대학교 약학연구소 2005 藥學論文誌 Vol.16 No.1

Analysis of acyl lysophosphatidic acids(LPAs) has clinical importance as a potential biomarker for ovarian and other gynecological cancers or obesity in the aspect of prevention. Here we report simple sample preparation and analytical method developed with high sensitivity and specificity for the early detection of gynecological cancers to improve the overall outcome of this disease. We established a novel quantification method of acyl-LPAs in plasma by electrospray negative ionization tandem mass spectrometry (MS-MS) using multiple reaction monitoring mode without conventional TLC step. Protein-bound lipids, acyl-LPAs in plasma were extracted with methanol : chloroform (2:1) containing LPA C14:0 as internal standard under acidic condition. Following back extraction with chloroform and water, centrifuged lower phase was evaporated and reconstituted in methanol and then analyzed. Using ESI-MS-MS with negative ionization MRM mode, all the species of LPAs were completely separated from plasma matrix without severe interferences. For MRM mode, Q1 ions selected were m/z 409, 433, 435, 437 and 457 which corresponds to molecular mass [M-H]- of C16:0, C18:2, C18:1, C18:0 and C20:3 LPA, respectively. Q2 ions selected for MRM was m/z 79, phosphoryl product. Using MS/MS with MRM mode, all the species of LPAa were completely separated from plasma matrix without severe interferences. This method allowed simultaneous detection and quantification of different species of LPAs in a plasma over a linear dynamic range of 0.01 - 25 (mol/L. The method detection limit was 0.3 pmol/ml with correlation coefficient of 0.9983 in most LPAs analyzed. When applied to the plasmas with normal and gynecological cancer patient, this new method differentiated two different groups by way of total LPA level.

보리순의 영양성분 조성

서혜지,최용민,김상천,이준수,이기택,정명근,천지연,황진봉,남진식,서우덕,황영,김행란,김세나 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

대리모계약규정을 위한 법률안의 제안

윤혜란 숭실대학교 법학연구소 2006 法學論叢 Vol.16 No.-

현대사회는 생명공학의 발달로 인공수정에 의하여 선천적 이유에서나, 후천적인 이유에 의하여 부부의 일방 또는 쌍방에 불임의 원인이 있으면 불가능 하였던 임신이 가능하게 되었다. 또한 능력 있는 미혼의 여성이나, 남편을 잃은 미망인 등 일반적으로 임신 할 수 없는 사람들도 아이를 원한다면 임신이 가능하여 출산할 수 있게 되었다. 인공수정의 방법 중 대리모에 의한 경우 각 나라들은 인간의 존엄과 사회질서를 파괴시킬 수 있다고 하여 불법으로 간주하고 엄격히 금해 왔지만, 불임 부부 등의 증가와 변화하는 가족관계 등의 영향으로 각 나라들은 사회적·윤리적·종교적 실정을 고려하여 법률로 규정 하고 있다. 우리의 경우 대리모에 관하여 명문의 규정도 없으며 민법 제103조의 선량한 풍속 기타 사회질서에 위반함을 이유로 대리모를 인정하지 않고 있다. 대리모 문제는 가족의 형태가 점차 혈연주의를 탈피하고 새로운 가족형태로 변화하고 있는 시점에서 혈연주의를 유지시키기 위한 시대에 역행하는 문제라는 비판이 될 수 있겠다. 하지만, 대리모를 부정한다는 것은 혈연관계를 중요하게 생각하는 우리나라의 경우 자녀를 얻기 위한 종족보존의 욕망을 지나치게 제한하는 것이라고 볼 수 있으며 불법행위를 조장하게 되는 문제를 내포하고 있는 것이다. 사회적 문화적으로 많은 변화를 거듭하고 있는 현실에서 국가가 불임부부들에게 대리모를 찾는 것을 민법 제103조 등에 의하여 무효로 보아 금지할 것이 아니라 대리모와 의뢰부부, 이미 출생한 자, 현재도 출생하고 있는 자, 출생을 앞둔 자들의 법률적 지위를 보호하기 위하여 법률을 제정해야 한다. With the development of bio-engineering in modern society, artificial insemination now makes pregnancy possible when one or both members of a couple are not fertile due to inborn or postnatal reasons. Also, single women or widows can now give birth to a child through this process. Depending on the body of the woman who is to be artificially inseminated, it can be grouped as inner artificial insemination, outer artificial insemination and surrogate mothers. Inner artificial insemination is when sperm is inserted into the woman's body, while outer artificial insemination is when the sperm and egg are inseminated inside a lab and then entered into the woman's body. Surrogate mothers are used when women who wish for a child, but cannot be pregnant through both inner and outer insemination methods, agrees with a surrogate mother to carry and give birth to the woman's child on their behalf. Though there are no problems with artificial insemination between couples, there are legal problems for non-couple artificial insemination. Moreover, there are particularly more problems when a surrogate mother is involved. Nations, according to their development of bio-engineering, have made legislations for artificial insemination depending on their social, religious, and ethical framework. However, in Korea there is still only the civil law article 844 on legitimacy of a child and legal custody that legislates this issue. According to the legality of child laws on artificial insemination and legal custody of them, Korea's judicial precedents of civil law article 844 have approved of it with no limitations, but in the 1980's it was recognized to be limited. However, the recent trends of judicial precedents have been recognizing blood relationships in a limited manner, and brings up the question of whether one's own profits are unconsidered and whether it prefers blood relationships, which is a retrograde step to practices of older times. In the case of surrogate mother agreements, most nations strictly forbid it as illegal because it can destroy people's dignity and social order. However, with evidence of infertility of a couple, and due to changing family relationships, surrogate mother agreements have been legally allowed to fit the social, ethical, and religious circumstances of that nation. Unfortunately, there are no related legislations as such in Korea. At a point when we are emerging from the importance of blood relationships, surrogate mother agreements can be following suit to a modern, but wrongful practice. However, denying surrogate mothers can be another way to make concubines, which was practiced in our history that placed great importance on blood relationships, evil and therefore should be approved in a limited manner.

노인시설을 위한 효율적 프로그램으로서의 원예활동 효과검증 : 관찰법에 의한 평가

곽혜란,이종석,Shannon E. Jarrot,Paula D. Relf 한국식물인간환경학회 2000 인간식물환경학회지 Vol.3 No.4

Therapetuic horticulture activities provide an ideal opportunity for engaging care participants with dementia that can be modified to suit a wide range of cognitive and functional abilities. As the results, therapeutic horticulture activities provide appropriate levels of stimulation, serve rehabilitation, new skill development and accomplishment with horticultural success. This study was conducted to validate the benefits of therapetuic horticulture activities as effective program of institutional care for elederly people, Three hypothesis was set up based on the charactieristics of elderly people and these hypothesis was verified by the observational method. Especially, non-horticulture activities(music, art, craft...) was observed to distinguish the effectiveness of horticulture activities. As the result of this study, the portion of time spent doing nothing during the horticulture activities was lower than during non-horticulture activities, the portion of participants engaged in their time during the horticulture activity engaged in horticulture behavior was greater than was spent in productive behavior during non-horticulture activities. However, the average levels of affect revelaed no differeces in affect between the horticultural and non-horticultural activities.

아동을 위한 원예와 자연에 대한 경험의 중요성

곽혜란,곽병화 한국식물인간환경학회 2000 인간식물환경학회지 Vol.3 No.2

Neglected results of knowing the value of human life, especially of urban inhabitants, have become quite obvious, will be more documnted repidly, and are more desirable than ever. Human-life quality does not just influence on upgrading materialistic and spiritual lives, but also badly needs emotional living. The present treatise discusses and deals with a concept of emotional experiences through activites in garden and nature at the earlier-age stage of children. Although the emotional life is attained by various with artistic and sports playings, and this enables to sustain the development of educational fulfillment, the education in nature not only is important for adults, but also is exceptionally so for younger children who can acquire much rewarding educational consequences in their later years of life. Horticultural activity is understood today to be the easiest and yet the closest way to have feeling and comprehend the realistic nature by especially uran children The impact is expected to be bettered through the activities of gardening and nature at the stage of childhood, and the influences may be eventually lasted up to the age stage of adult. This would, in turn, have a great significace to the development of social welfare and natioal prosperity.

T-집단에서 피이드백유무에 따른 회기별 신뢰행동의 변화

최혜란,설기문 東亞大學校 學生生活硏究所 1998 學生硏究 Vol.17 No.-

The Purpose of this study was to research changes in trusting behavior in T-group counseling. The subjects of this study were 40 students of K and D unversities in the city of Pusan, who were divided into 4 groups which experienced 18 hours of group counseling. There were 18 male and 22 female students. The instruments of this study were "Expectation About Counseling" (Park, 1985), "Group Behaviour Questionnaire", and "Trust Diagram". At the end of each session the trusting behaviour was immediately measured by the filling out of the self-perception and other~perception assessment forms, and the writing out of the verbarim record of the proceedings which had been recorded during group sessions. From the results and discussions of this research, the following conclusions were derived. 1. The trusting behaviour of members in the T-group counseling increases according to group sessions. In other words, trusting behaviour develops from the beginning of the group. 2. The trusting behaviour of group members doesn't change significantly according to feedback. The growth of trusting behaviour increases as the sessions progress whether there is feedback or not. In addition, feedback does no have may effect on the change in trusting behaviour. 3. The verbatum record of the proceeding of the counseling sessions shows that group trusting behaviour significantly increases after the second session. Therefore, group leaders should put into practice group activities based on mutual confidence among the group members' development of trusting behavior of group members in T-group counseling is not significantly affected by the level of the members.

주택의 외관형태가 지니는 이미지에 관한 연구

김혜란,강경호,손세관 대한건축학회 2004 대한건축학회논문집 Vol.20 No.12

The aim of this study is to figure out how people recognize the Image of house. The research is based on the approach of cognitive-psychology, which tries to understand the response of human beings toward their environment in term of psychological mechanism. The outcomes from this study are as follows. First, as the result of the factor analysis for the 12 perceptual scales, 3 major factors were extracted. These 3 factors are proved to be the dimension of esthetics and the dimension of ornament, and the dimension of modern. Second, the exterior form of house could be classified into 8 patterns by the 3 dimensions of the perceptual image. Third, the co-relationship between the conceptual and taste images that the exterior form of house has shown in this research, it was found that most respondents more tend to line in the house-looking building than in the non house-looking building. Fourth, it was found that people have more familiar and comfortable Image to simple designed house with hipped roof. This trend has shown to be one of common tendencies that people may have when they think of the image of house. In addition, roof has played the most important role in putting the image of house into people it was found that the most favorite house to the respondents has the esthetic and modern images that the roof shape is not seen in outside and the exterior wall is mainly emphasized. Fifth, the most ideal image of house seems to be sensually beautiful, and not flamboyantly decorated. In addition, it needs to cause the feeling of house-looking and comfort from people and then non-ideal image of house doesn't have any esthetical aspect. Rather, it has idiosyncratic image in general.

장애아동을 위한 원예치료 : Effect of HT as a Altemative Therapy, Especially in the United States 대체치료의 일환으로서의 원예치료, 미국의 사례를 중심으로

곽혜란,Relf, Paula Diane 韓國植物ㆍ人間ㆍ環境學會 2000 인간식물환경학회지 Vol.3 No.1

The disability has meaning of either physical or mental impairment restricting motality, communication, self-care, and interpersonal skills, etc., and requires manifold levels of rehabilitation system. Especially in the case of disabled children, the disability may result in causing serious problems of reduction in much potentialities they deserve in the early stage of development. Children's disability includes trauma of cerebral palsy, spinabifida, neurogical, spiral cord, and brain impairments, etc. The problem of theses disabilities arise in muscle motors such as fine-and gross-motor skills, the coordination as visual motor cooperation, or in cognition of maintaining the concentration ability, or of long and short memorizings. Various alternative therapies must be needed for the children in order to gain their rehabilitation and social restoration influences, and are recently common in the United States. Horticultural therapy being one of them is known to have noted benefits for the disabled with multi-motor skills, responsibility for taking care of raising plants, cognitive skills of self-esteem, and motivation. Horticultural therapy either alone in combination with other alternative means as music or pet therapy for the disabled children have also been documented often, and known to increase subscaling in depression, hyperactivity, social interaction, depression, anxiety and intellectual screening. Horticultural activities by the disabled children are likewise supposed to provide them with a wise range of educational opportunities through various practical hand-on experiences. These information of the beneficial activity are the example of Rusk Institute of Rehabilitation Medicine and Kluge Children's Rehabilitation Center recently operating in the United State.

枕肱 한시에 나타난 「수행의 반려자」로서의 달

정혜란 한국고시가문학회 2005 한국시가문화연구 Vol.0 No.15

Chimgweng was a Buddhist priest. He lived in the 17th century. He wrote 119 poems, 4 libretto, and 27 writings. This study is on his chinese poems, especially the image of the moon appears in his chines poems. Chimgweng's chinese poem is buddhist literature and has buddhistic color, but it is beautiful as a lyric poem. The moon appears forty-seven times in his poems. The moon, in his chines poems, comes out as a varied appearance and comes in sight in the evening, sometimes in the midnight, sometimes early in the morning. This study aims to make a study of the image of the moon, appeared in the his poems. Chimgweng train himself Zen. Therefore he stayed at a small room in temple or a valley alone. There was no person around him. He has no teather. He has no friend. He felt lonely and sad. Sometimes he met his friends, but had to go back to the temple soon. Separation from his friends made him sad. Or he was loath to part greatly grieved over the death of his friends. At the moment the moon consoled him. Chimgweng wrote a poem about this moon. In conclusion, the moon which appears in Chimgweng's chinese poems has a image of a teather, or a companion, or a friend.

여대생의 체위, 식습관 및 영양실태 조사

신혜란,박영미 효성여자대학교 가정대학 학도호국단 1987 家政大論集 Vol.6 No.-

본 연구에서는 효성여대 3, 4학년 학생 96명을 대상으로 여대생들의 바람직한 건강관리와 영양지도를 위해서 이들의 체위와 식습관 영양소 섭취상태를 조사하여 다음과 같은 결과를 얻었다. (1) 본 조사 대상자들의 체위는 신장 160∼162㎝가 전체의 24%로 가장 많았고 154∼156㎝가 20%, 158∼160㎝가 17%의 분포를 보였고 평균치는 158.2㎝로 나타났다. 체중은 47.5∼50㎏이 24%, 50∼52.5㎏이 17%, 45∼47.5, 52.5∼55㎏이 15%의 분포를 보였고 평균치는 49.7㎏으로 나타났다. Skinfold Thickness는 16∼18㎜24%, 10∼12㎜, 12∼14㎜가 15%, 18∼20㎜는 11%의 분포를 나타났고 이들의 평균치는 17.1㎜였다. (2) 식습관 평균점수는 10점 만점에서 4.44였고 식습관을 다시 5등급으로 분류 했을 때 다소 불량으로 판단되는 2등급에 속하였다. (3) 영양소 섭취상태는 열량 1431.1㎉, 단백질 55.1gm, 철 14.7㎎으로서 권장량에 비해 부족상태이고 칼슘, 아스코르빈산, 티아민, 리보플라빈, 나이아신은 각각 559.5㎎, 52.7㎎, 1.1㎎, 1.2㎎, 16.6㎎으로서 권장량과 거의 일치하므로서 모두 양호한 편이다. 총 열량 구성에 있어서 당질 단백질 지방이 차지하는 비율을 보면 단백질은 55gm로 15.4%를 차지하고 지방은 30.8gm로 19.5%를 차지하고 당질은 총 열량중 65%를 차지하는 것으로 나타났다. For the purpose of implementing health and nutrition education for college women, we investigatednutritional status of college women related to health status, food habit and nutrient intake. The survey was conducted for ten days, from November 20 to 30, 1986. The subjects of this survey were 96 students of Hyosung women's University. The results of health status, food habit and nutrient intake of college womens were as follows; 1. Average height, weight and skinfoldthinckness were height(158.2cm) weight(49.7kg) skinfold-thickness(17.1mm). 2. Average nutrient intake of college women junior sinior per day. Calorie intake was 1431kcal. This was composed of carbohydrate(65%), protein 55.1gm(15.4%), and fat 30.8gm(19.5%) other nutrients such as calcium(559.5gm) iron(147mg), vitamin A(5104.9I.U.) and Thiamin(1.1mg) Riboflavin(1.2mg) Niacin(16.6mg) ascorbicacid(52.7mg).