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Highly Porous Cellulose Microbeads and their Adsorption for Methylene Blue
Jiarui Hua,Ranju Meng,Tiejun Wang,Huiying Gao,Zhenze Luo,Yuanyuan Jin,Lin Liu,Juming Yao 한국섬유공학회 2019 Fibers and polymers Vol.20 No.4
Highly porous cellulose microbeads with porosity of more than 90 % were successfully prepared via a faciledissolution and subsequent regeneration of cellulose in H2SO4/Na2SO4 coagulation bath. Effects of coagulation temperature,H2SO4, and Na2SO4 concentration on the microstructure, average diameter, porosity, specific surface area, and mechanicalproperty of formed cellulose beads were investigated systematically. In view of the high porosity and specific area, thecellulose beads were used as adsorbents for dye removal. The results revealed that the porous cellulose beads exhibited highadsorption performance with maximum adsorption capacity of 48.80 mg/g for cationic dye methylene blue. Besides, theporous cellulose beads also presented a potential in practical application and its adsorption capacity for methylene blue stillretained 29.43 mg/g after six adsorption-desorption cycles.
RNAi-Mediated Gene Silencing of Trcot1 Induces a Hyperbranching Phenotype in Trichoderma reesei
Fei Gao,Mengzhu Li,Weiquan Liu,Yingguo Bai,Tu Tao,Yuan Wang,Jie Zhang,Huiying Luo,Bin Yao,Huoqing Huang,Xiaoyun Su 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.2
Trichoderma reesei is the major filamentous fungus used to produce cellulase and there is huge interest in promoting its ability to produce higher titers of cellulase. Among the many factors affecting cellulase production in T. reesei, the mycelial phenotype is important but seldom studied. Herein, a close homolog of the Neurospora crassa COT1 kinase was discovered in T. reesei and designated TrCOT1, which is of 83.3% amino acid sequence identity. Functional disruption of Trcot1 in T. reesei by RNAi-mediated gene silencing resulted in retarded sporulation on potato dextrose agar and dwarfed colonies on minimal medium agar plates containing glucose, xylan, lactose, xylose, or glycerol as the sole carbon source. The representative mutant strain, SUS2/Trcot1i, also displayed reduced mycelia accumulation but hyperbranching in the MM glucose liquid medium, with hyphal growth unit length values decreased to 73.0 μm/tip compared to 239.8 μm/tip for the parent strain SUS2. The hyperbranching phenotype led to slightly but significantly increased cellulase secretion from 24 to 72 h in a batch culture. However, the cellulase production per unit of mycelial biomass was much more profoundly improved from 24 to 96 h.