Inhibition of Microcystis aeruginosa by the Extracellular Substances from an Aeromonas sp

( Yu Mei Liu ),( Ming Jun Chen ),( Meng Hui Wang1 ),( Rui Bao Jia ),( Li Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.9

Growth of Microcystis aeruginosa could be inhibited significantly within 24 h by the extracellular substances prepared from Aeromonas sp. strain FM. During the treatment, the concentration of extracellular soluble carbohydrates increased significantly in algal culture. Morphological and ultrastructural changes in M. aeruginosa cells, including breakage of the cell surface, secretion of mucilage, and intracellular disorganization of thylakoids, were observed. HPLC-MS analysis showed that the extracellular substances of Aeromonas sp. strain FM were a mixture of free amino acids, tripeptides, and clavulanate. Among these, the algaelysis effects of lysine and clavulanate were confirmed.

Clinical Evaluation of Human Papillomavirus Detection by careHPV^TM Test on Physician-Samples and Self-Samples using The Indicating FTA Elute® Card

Wang, Shao-Ming,Hu, Shang-Ying,Chen, Feng,Chen, Wen,Zhao, Fang-Hui,Zhang, Yu-Qing,Ma, Xin-Ming,Qiao, You-Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Objective: To make the clinical evaluation of a solid-state human papillomavirus (HPV) sampling medium in combination with an economical HPV testing method ($careHPV^{TM}$) for cervical cancer screening. Methods: 396 women aged 25-65 years were enrolled for cervical cancer screening, and four samples were collected. Two samples were collected by woman themselves, among which one was stored in DCM preservative solution (called "liquid sample") and the other was applied on the Whatman Indicating FTA $Elute^{(R)}$ card (FTA card). Another two samples were collected by physician and stored in DCM preservative solution and FTA card, respectively. All the samples were detected by $careHPV^{TM}$ test. All the women were administered a colposcopy examination, and biopsies were taken for pathological confirmation if necessary. Results: FTA card demonstrated a comparable sensitivity of detecting high grade Cervical Intraepithelial Neoplasia (CIN) with the liquid sample carrier for self and physician-sampling, but showed a higher specificity than that of liquid sample carrier for self-sampling (FTA vs Liquid: 79.0% vs 71.6%, p=0.02). Generally, the FTA card had a comparable accuracy with that of Liquid-based medium by different sampling operators, with an area under the curve of 0.807 for physician &FTA, 0.781 for physician &Liquid, 0.728 for self & FTA, and 0.733 for self &Liquid (p>0.05). Conclusions: FTA card is a promising sample carrier for cervical cancer screening. With appropriate education programmes and further optimization of the experimental workflow, FTA card based self-collection in combination with centralized $careHPV^{TM}$ testing can help expand the coverage of cervical cancer screening in low-resource areas.

Physical Layer Security in Heterogeneous Cellular Networks

Hui-Ming Wang,Tong-Xing Zheng,Jinhong Yuan,Towsley, Don,Moon Ho Lee IEEE 2016 IEEE TRANSACTIONS ON COMMUNICATIONS Vol.64 No.3

<P>The heterogeneous cellular network (HCN) is a promising approach to the deployment of 5G cellular networks. This paper comprehensively studies physical layer security in a multitier HCN where base stations (BSs), authorized users, and eavesdroppers are all randomly located. We first propose an access threshold-based secrecy mobile association policy that associates each user with the BS providing the maximum truncated average received signal power beyond a threshold. Under the proposed policy, we investigate the connection probability and secrecy probability of a randomly located user and provide tractable expressions for the two metrics. Asymptotic analysis reveals that setting a larger access threshold increases the connection probability while decreases the secrecy probability. We further evaluate the network-wide secrecy throughput and the minimum secrecy throughput per user with both connection and secrecy probability constraints. We show that introducing a properly chosen access threshold significantly enhances the secrecy throughput performance of a HCN.</P>

Consensus Problem of Second-order Multi-agent Systems with Communication Channel Constraint on Signal Amplitude (ICCAS 2012)

Ming-Hui Wang,Kenko Uchida 제어로봇시스템학회 2012 제어로봇시스템학회 국제학술대회 논문집 Vol.2012 No.10

This paper investigates the consensus problem of the second-order multi-agent systems with communication channel constraint on signal amplitude. Communication delays as one kind of channel constraints in the second-order multi-agent systems have been discussed in the recent literature while in this paper consensus problem of second-order multi-agent systems with communication channel constraint on signal amplitude is investigated. A state-dependent switching parameter is introduced into the consensus protocol. A sufficient condition is obtained for the consensus problem of the second-order multi-agent systems with communication channel constraint based on our early work. A simple example is also given to verify the effectiveness of the proposed theorem.

Transiting Exoplanet Monitoring Project (TEMP). I. Refined System Parameters and Transit Timing Variations of HAT-P-29b

Wang, Songhu,Wang, Xian-Yu,Wang, Yong-Hao,Liu, Hui-Gen,Hinse, Tobias C.,Eastman, Jason,Bayliss, Daniel,Hori, Yasunori,Hu, Shao-Ming,Li, Kai,Liu, Jinzhong,Narita, Norio,Peng, Xiyan,Wittenmyer, R. A.,Wu American Astronomical Society 2018 The Astronomical journal Vol.156 No.4

Prognostic Significance of α5β1-integrin Expression in Cervical Cancer

Wang, Hua-Yi,Chen, Zhe,Wang, Zhu-Hui,Wang, Hong,Huang, Li-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

The purpose of this study was to evaluate the association of expression of ${\alpha}5{\beta}1$-integrin with clinicopathologic features and prognosis in cervical cancer. Levels of ${\alpha}5{\beta}1$-integrin in normal cervical mucosa and cervical cancer tissue were detected with immunohistochemistry. Survival analysis by the Kaplan-Meier method was performed to assess prognostic significance. ${\alpha}5{\beta}1$-integrin expression was detected in 84.6% (143/169) cervical cancer samples, significantly different from that in normal cervical mucosa (P < 0.05). Positive expression rates of ${\alpha}5{\beta}1$-integrin in patients with poor histologic differentiation, lymph node metastasis, and recurrence were elevated. Using Kaplan-Meier analysis, a comparison of survival curves of low versus high expression of ${\alpha}5{\beta}1$-integrin revealed a highly significant difference in human cervical cancer cases (P < 0.05), suggesting that overexpression of ${\alpha}5{\beta}1$-integrin is associated with a worse prognosis.The ${\alpha}5{\beta}1$-integrin promotes angiogenesis and associates with lymph node metastasis, vascular invasion and poor prognosis of cervical cancer. The current study indicated that ${\alpha}5{\beta}1$-integrin may be an independent prognostic factor for cervical cancer patients.

Distributed Beamforming for Physical-Layer Security of Two-Way Relay Networks

Hui-Ming Wang,Qinye Yin,Xiang-Gen Xia IEEE 2012 IEEE transactions on signal processing Vol.60 No.7

<P>In this paper, we address the security of a two-way relay network in the presence of an eavesdropper, where each node is only equipped with single antenna. We propose two-phase distributed analog network coding, or distributed beamforming and power allocation to enhance the secrecy sum rate of the data exchange. In the first phase, the two terminals broadcast their information data simultaneously to all the relay nodes. In the second phase, three different security schemes are proposed: optimal beamforming, null-space beamforming, and artificial noise beamforming. In the first scheme, the objective is to achieve the maximum secrecy sum rate of the two terminals. Mathematically, the objective function is difficult to optimize. In the second scheme, we maximize the total information exchanged while we eliminate the information leakage completely, subject to the total transmission power constraint. We show that the problem has a unique and global optimum, which can be solved using bisection method. When the instantaneous channel state information of the eavesdropper is not available, we propose an artificial noise beamforming in the third scheme. We minimize the information transmission power so that the artificial noise power is maximized to eliminate information leakage, under the constraints of quality of service (QoS) required by terminals. It is a second-order convex cone programming (SOCP) problem, thus can be efficiently solved using interior point methods. Numerical results are provided and analyzed to show the properties and efficiency of the proposed designs.</P>

Function of Global Regulator CodY in Bacillus thuringiensis BMB171 by Comparative Proteomic Analysis

( Ming Xia Qi ),( Fei Mei ),( Hui Wang ),( Ming Sun ),( Ge Jiao Wang ),( Ziniu Yu ),( Yeon Ho Je ),( Ming Shun Li ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.2

CodY is a highly conserved protein in low G+C gram-positive bacteria that regulates genes involved in sporulation and stationary-phase adaptation. Bacillus thuringiensis is a grampositive bacterium that forms spores and parasporal crystals during the stationary phase. To our knowledge, the regulatory mechanism of CodY in B. thuringiensis is unknown. To study the function of CodY protein in B. thuringiensis, BMB171codY- was constructed in a BMB171 strain. A shuttle vector containing the ORF of cry1Ac10 was transformed into BMB171 and BMB171codY-, named BMB171cry1Ac and BMB171codY-cry1Ac, respectively. Some morphological and physiological changes of codY mutant BMB171codY-cry1Ac were observed. A comparative proteomic analysis was conducted for both BMB171codY-cry1Ac and BMB171cry1Ac through two-dimensional gel electrophoresis and MALDI-TOF-MS/MS analysis. The results showed that the proteins regulated by CodY are involved in microbial metabolism, including branched-chain amino acid metabolism, carbohydrate metabolism, fatty acid metabolism, and energy metabolism. Furthermore, we found CodY to be involved in sporulation, biosynthesis of poly-β-hydroxybutyrate, growth, genetic competence, and translation. According to the analysis of differentially expressed proteins, and physiological characterization of the codY mutant, we performed bacterial one-hybrid and electrophoretic mobility shift assay experiments and confirmed the direct regulation of genes by CodY, specifically those involved in metabolism of branched-chain amino acids, ribosomal recycling factor FRR, and the late competence protein ComER. Our data establish the foundation for in-depth study of the regulation of CodY in B. thuringiensis, and also offer a potential biocatalyst for functions of CodY in other bacteria.

Fast Kalman Equalization for Time-Frequency Asynchronous Cooperative Relay Networks With Distributed Space-Time Codes

Hui-Ming Wang,Qinye Yin,Xiang-Gen Xia IEEE 2010 IEEE Transactions on Vehicular Technology VT Vol.59 No.9

<P>Cooperative relay networks are inherently time and frequency asynchronous due to their distributed nature. In this correspondence, we propose a transceiver scheme to combat both time and frequency offsets for cooperative relay networks with multiple relay nodes. At the relay nodes, a distributed linear convolutive space-time coding is adopted, which has the following advantages: 1) Full cooperative diversity can be achieved using a minimum mean square error (MMSE) or MMSE decision feedback equalizer (MMSE-DFE) detector, instead of a maximum-likelihood receiver when only time asynchronism exists. 2) The resultant equivalent channel possesses some special structure, which can be exploited to reduce the equalization complexity at the destination node. By taking full advantage of such a special structure, fast Kalman equalizations based on linear MMSE and MMSE-DFE are proposed for the receiver, where the estimation of the state vector (information symbols) can be recursively taken and become very computationally efficient, compared with direct equalization. The proposed scheme can achieve considerable diversity gain with both time and frequency offsets and applies to frequency-selective fading channels.</P>

Cluster Consensus of Multi-agent Systems with Communication Delay (ICCAS 2013)

Ming-Hui Wang,Kenko Uchida 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10

Cluster consensus problem of the multi-agent systems with communication delay is studied in this paper. We consider the multi-agent systems with a special Laplacian matrix as its system matrix. For two types of protocols with time-delay, our work shows that the multi-agent systems with communication delays can reach cluster consensus. Some numerical simulations are given to verify the effectiveness of the theoretical results.