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Function of Global Regulator CodY in Bacillus thuringiensis BMB171 by Comparative Proteomic Analysis
( Ming Xia Qi ),( Fei Mei ),( Hui Wang ),( Ming Sun ),( Ge Jiao Wang ),( Ziniu Yu ),( Yeon Ho Je ),( Ming Shun Li ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.2
CodY is a highly conserved protein in low G+C gram-positive bacteria that regulates genes involved in sporulation and stationary-phase adaptation. Bacillus thuringiensis is a grampositive bacterium that forms spores and parasporal crystals during the stationary phase. To our knowledge, the regulatory mechanism of CodY in B. thuringiensis is unknown. To study the function of CodY protein in B. thuringiensis, BMB171codY- was constructed in a BMB171 strain. A shuttle vector containing the ORF of cry1Ac10 was transformed into BMB171 and BMB171codY-, named BMB171cry1Ac and BMB171codY-cry1Ac, respectively. Some morphological and physiological changes of codY mutant BMB171codY-cry1Ac were observed. A comparative proteomic analysis was conducted for both BMB171codY-cry1Ac and BMB171cry1Ac through two-dimensional gel electrophoresis and MALDI-TOF-MS/MS analysis. The results showed that the proteins regulated by CodY are involved in microbial metabolism, including branched-chain amino acid metabolism, carbohydrate metabolism, fatty acid metabolism, and energy metabolism. Furthermore, we found CodY to be involved in sporulation, biosynthesis of poly-β-hydroxybutyrate, growth, genetic competence, and translation. According to the analysis of differentially expressed proteins, and physiological characterization of the codY mutant, we performed bacterial one-hybrid and electrophoretic mobility shift assay experiments and confirmed the direct regulation of genes by CodY, specifically those involved in metabolism of branched-chain amino acids, ribosomal recycling factor FRR, and the late competence protein ComER. Our data establish the foundation for in-depth study of the regulation of CodY in B. thuringiensis, and also offer a potential biocatalyst for functions of CodY in other bacteria.
Direct ethanol production from dextran industrial waste water by Zymomonas mobilis
Ming-xiong He,Han Qin,Xiao-bo Yin,Zhi-yong Ruan,Fu-rong Tan,Bo Wu,Zong-xia Shui,Li-chun Dai,Qi-chun Hu 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.11
The direct production of ethanol from dextran industrial waste water was investigated by using Zymomonasmobilis via batch and semi-continuous fermentation mode. In batch fermentation, pretreated waste water (unsterilizedand sterilized), pH value (3.8 and 6.0), and Mg2+(with and without) was compared with OD600, sugar and ethanol con-centration. After 24 h fermentation, sugar in the dextran waste water was almost exhausted, and the amount of ethanolaccumulated reached 24.33-29.92 g/l, which is nearly 99% of the theoretical yield of ethanol. Kinetic parameters ofZ. mobilis in batch fermentation were also investigated. The raw dextran waste water was also used in semi-continuousfermentation. After 48 h fermentation, the production of ethanol was 28.65 g/l. These results indicated that dextranwaste water may be used as a candidate substrate and Z. mobilis could convert the raw material into ethanol directly.
Ding-Ming Xue,Shi-Chao Qi,Xin Liu,Yu-Xia Li,Xiao-Qin Liu,Lin-Bing Sun 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-
N-doped porous carbon-based materials (NPCMs) with hierarchical pore structures have beenconsidered to be a suitable alternative to meet the ever-increasing demands for supercapacitors;however, the universally low yield of the NPCMs has restricted their practical applications. Herein, aseries of NPCMs with hierarchical pore structures are synthesized with significantly increased yieldsthrough the carbonization of the copolymer made from 2,4,6-tris(chloromethyl)mesitylene and pphenylenediamine. The development of the hierarchical pore structures and the N content of the NPCMsshow opposite dependences on the increasing carbonization temperature. The NPCM exhibits the bestcapacitive ability only if the sufficiently developed hierarchical structures and moderate N content areachieved simultaneously. Therefore, NPCM-600 that is carbonized at 600 C with an excellent yield of53.6% (wt.), large specific surface area of 1778 m2 g 1, and N content of 4.13% (wt.) yields an ideal specificcapacitance of 298 F g 1 at the current density of 1 A g 1 and a perfect cycling stability of the capacitanceafter 10,000 cycles at 10 A g 1. The yield of the NPCM-600 is considerably higher than those for manyother recently reported NPCMs. NPCM-600 also shows better capacitance than those of the otherreported NPCMs, such as NOPC-bis-CN-3 (167 F g 1) and CHCPB-K-600 (260 F g 1).
Dao-Zong Xia,Xin-Fen Yu,Hui-Ming Wang,Qi-Ya Ren,Bing-Mei Chen 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.4
To investigate the anti-obesity and hypolipidemic effects of Alpinia officinarum ethanolic extract (AOE) for the first time, anti-obesity models in vivo were used. Ten male Sprague-Dawley rats were fed normal control diet (NC); other groups of rats were fed a high-fat diet (HFD) with or without different proportions of AOE (AOE-1, 3%; AOE-2, 5%) for 6 weeks to examine feed intake, body and adipose tissue weight, serum total cholesterol (Total-C), triacylglycerol (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and leptin levels, alanine aminotransferase (ALT) and aspartate aminotranferase activities, hepatic Total-C and TG levels, and the pathological changes in liver and epididymal adipose tissues. Interestingly, feed intakes among the experimental groups were not significantly different. Body weight gains were significantly lowered in the AOE-1 and AOE-2 groups compared with the HFD group (P<.05) and near to the level of the NC group. AOE also improved the lipid profile in serum and the pathological changes in liver and adipose tissue and decreased the relative weights of epididymal and perirenal white adipose tissues. They improved lipid profile by lowering serum Total-C, TG, and LDL-C concentrations, leptin content, and the atherogenic index compared with the HFD group. The HDL-C concentration and the ratio of HDL-C/Total-C significantly increased compared with those of the HFD group. The serum ALT activity of the AOE-2 group was notably lower than that of the HFD group. Our data suggest that AOE can be considered as an anti-obesity agent that is effective for suppressing body weight gain and decreasing lipid profile.
Preparation and Gas Permeability of ZIF-7 Membranes Prepared via Two-step Crystallization Technique
Fang Li,Qi Ming Li,Xin Xia Bao,Jian Zhou Gui,Xiao Fei Yu 한국화학공학회 2014 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.52 No.3
Abstract - Continuous and dense ZIF-7 membranes were successfully synthesized on α-Al2O3 porous substrate via two-step crystallization technique. ZIF-7 seeding layer was first deposited on porous α-Al2O3 substrate by in-situ low temperature crystallization, and then ZIF-7 membrane layer can be grown through the secondary high-temperature crystallization. Two synthesis solutions with different concentration were used to prepare ZIF-7 seeding layer and membrane layer on porous α-Al2O3 substrate, respectively. As a result, a continuous and defect-free ZIF-7 membrane layer can be prepared on porous α-Al2O3 substrate, as confirmed by scanning electron microscope. XRD characterization shows that the resulting membrane layer is composed of pure ZIF-7 phase without any impurity. A single gas permeation test of H2, O2, CH4 or CO2 was conducted based on our prepared ZIF-7 membrane. The ZIF-7 membrane exhibited excellent H2 molecular sieving properties due to its suitable pore aperture and defect-free membrane layer.
Efficacy and Safety of Bevacizumab in Chinese Patients with Metastatic Colorectal Cancer
Zhu, Li-Ming,Zhao, Ya-Zhen,Ju, Hai-Xing,Liu, Lu-Ying,Chen, Lei,Liu, Bi-Xia,Xu, Qi,Luo, Cong,Ying, Jie-Er,Yang, Yun-Shan,Zhong, Hai-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16
Objective: To evaluate the efficacy and safety of bevacizumab in the treatment of patients with metastatic colorectal cancer (mCRC). Methods: In a single-center, observational study of 91 Chinese patients with mCRC who received bevacizumab in combination with chemotherapy was conducted. Objective response rates (ORRs), progression-free survival (PFS), overall survival (OS) and adverse events were recorded, and the relationships between various clinical factors and PFS or OS were evaluated by Cox proportional hazards models. Results: Treatment with bevacizumab and chemotherapy was effective and tolerable. Univariate analysis showed that PFS and OS were significantly associated with the Eastern Cooperative Oncology Group performance status (ECOG-PS) score, duration of bevacizumab exposure, and whether chemotherapy was continued after discontinuation of bevacizumab treatment. A multivariate analysis showed that the duration of bevacizumab exposure and whether chemotherapy was continued after discontinuation of bevacizumab were independent prognostic factors for PFS and OS. Conclusion: In Chinese mCRC population, the shorter the duration of exposure to bevacizumab and chemotherapy, the worse the prognosis is.
Rajbhandary, S.,Zhao, Ming-Feng,Zhao, Nan,Lu, Wen-Yi,Zhu, Hai-Bo,Xiao, Xia,Deng, Qi,Li, Yu-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10
Background/Objectives: Maintenance of cellular function in culture is vital for transfer and development following adoptive immunotherapy. Dual properties of IL-21 in activating T cells and reducing activation induced cell death led us to explore the mechanism of action of IL-21 enhanced proliferation and cytotoxic potential of CIK cells. Method: CIK cells cultured from PBMCs of healthy subjects were stimulated with IL-21 and cellular viability and cytotoxicity to K562 cells were measured. To elucidate the mechanism of action of IL-21, mRNA expression of cytotoxic factors was assessed by RT-PCR and protein expression of significantly important cytotoxic factors and cytokine secretion were determined through flow cytometry and ELISA. Western blotting was performed to check the involvement of the JAK/STAT pathway following stimulation. Results: We found that IL-21 did not enhance in vitro proliferation of CIK cells, but did increase the number of cells expressing the CD3+/CD56+ phenotype. Cytotoxic potential was increased with corresponding increase in perforin ($0.9831{\pm}0.1265$ to $0.7592{\pm}0.1457$), granzyme B ($0.4084{\pm}0.1589$ to $0.7319{\pm}0.1639$) and FasL ($0.4015{\pm}0.2842$ to $0.7381{\pm}0.2568$). Interferon gamma and TNF-alpha were noted to increase ($25.8{\pm}6.1ng/L$ to $56.0{\pm}2.3ng/L$; and $5.64{\pm}0.61{\mu}g/L$ to $15.14{\pm}0.93{\mu}g/L$, respectively) while no significant differences were observed in the expression of granzyme A, TNF-alpha and NKG2D, and NKG2D. We further affirmed that IL-21 signals through the STAT-3 and STAT-5b signaling pathway in the CIK cell pool. Conclusion: IL-21 enhances cytotoxic potential of CIK cells through increasing expression of perforin, granzyme B, IFN-gamma and TNF-alpha. The effect is brought about by the activation of STAT-3 and STAT-5b proteins.