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Hop, Huynh Tan,Arayan, Lauren Togonon,Huy, Tran Xuan Ngoc,Reyes, Alisha Wehdnesday Bernardo,Min, WonGi,Lee, Hu Jang,Park, Soo Jong,Chang, Hong Hee,Kim, Suk Elsevier 2018 Vaccine Vol.36 No.21
<P><B>Abstract</B></P> <P>In this study, we assessed the protective efficacy of single subunit vaccines, encoded by the <I>B. abortus</I> 544 genes <I>aspC</I>, <I>dps</I>, <I>yaeC</I> and <I>inpB</I>, against <I>B. abortus</I> infection in mice. First, immunization with these antigens, with the exception of the YaeC protein, was found to elicit both humoral and cellular immune responses with IgG2a being dominant over IgG1. In addition, a massive production of IFN-γ but lower degree of IL-10 was observed, suggesting that all three antigens were able to induce predominantly cell-mediated immunity in response to <I>B. abortus</I> infection. Further investigation of a combined subunit vaccine (CSV) consisting of purified AspC, Dps, InpB and Ndk proteins showed a superior protective effect in mice against brucellosis. The intraperitoneal injection of this combination was shown to induce a remarkable production of IFN-γ and IL-2, which occurred in conjunction with an increase of blood CD4<SUP>+</SUP> and CD8<SUP>+</SUP> T cell proportions. In addition, the higher titer of IgG2a compared to IgG1 elicited by this CSV was obtained, suggesting that this CSV induced a typical T-helper-1-dominated immune response <I>in vivo</I>. Furthermore, the protection level induced by this combination was significantly higher than that induced by single antigens and was not significantly different compared to a group immunized with a live attenuated vaccine (RB51). Altogether, our findings suggest that the combination of different immunogenic antigens could be a useful approach for the development of a new, effective and safe brucellosis vaccine that can replace current vaccine strains.</P>
Hop, H.T.,Arayan, L.T.,Simborio, H.L.,Reyes, A.W.B.,Min, W.,Lee, H.J.,Lee, J.J.,Chang, H.H.,Kim, S. Pergamon Press 2016 Comparative immunology, microbiology and infectiou Vol.45 No.-
<P>To date, detection of antibodies against the lipopolysaccharide portion is the backbone of most serodiagnostic methods for brucellosis screening. However this pose a risk for false positive reactions related to other pathogens especially that of Yersinia enterocolitica 0:9 which has the most prominent cross reactivity with Brucella spp. In this study, cloning and expression of Brucella abortus bacterioferritin (Bfr) was accomplished by PCR amplification into an expression vector system, and purification of a recombinant B. abortus Bfr (rBfr). The immunogenicity of rBfr was confirmed by Western blot with Brucella-positive bovine serum. To determine whether rBfr has a potential benefit for use in the serodiagnosis of bovine brucellosis, rBfr-based ELISA was performed. Interestingly, rBfr was able to detect anti-Brucella antibodies in positive sera in a dependent manner of TAT values but did not show an immunoreaction with negative samples. Particularly, average OD492 values at the lowest, medium and highest TAT titer levels were 1.4, 2.2 and 2.6-fold increase compared with the cutoff value, respectively. The accuracy, specificity and sensitivity of rBfr showed 89.09%, 93.6% and 85.33%, respectively. These findings suggest that rBfr might be a good candidate for serological diagnosis development of bovine brucellosis. (C) 2016 Elsevier Ltd. All rights reserved.</P>
Hop, Huynh Tan,Reyes, Alisha Wehdnesday Bernardo,Huy, Tran Xuan Ngoc,Arayan, Lauren Togonon,Min, WonGi,Lee, Hu Jang,Rhee, Man Hee,Chang, Hong Hee,Kim, Suk American Society for Biochemistry and Molecular Bi 2018 The Journal of biological chemistry Vol.293 No.9
<P>Brucella abortus is a Gram-negative zoonotic pathogen for which there is no 100% effective vaccine. Phagosomes in B. abortus infected cells fail to mature, allowing the pathogen to survive and proliferate. Interleukin 10 (IL10) promotes B. abortus persistence in macrophages by mechanisms that are not fully understood. In this study, we investigated the regulatory role of IL10 in the immune response to B. abortus infection. B. abortus infected macrophages were treated with either IL10 s1RNA or recombinant IL10 (rIL10), and the expression of phagolysosome- or inflammation-related genes was evaluated by qRT-PCR and Western blotting. Phagolysosome fusion was monitored by fluorescence microscopy. We found that the synthesis of several membrane-trafficking regulators and lysosomal enzymes was suppressed by IL10 during infection, resulting in a significant increase in the recruitment of hydrolytic enzymes by Brucella-containing phagosomes (BCPs) when IL10 signaling was blocked. Moreover, blocking IL10 signaling also enhanced proinflammatory cytokine production. Finally, concomitant treatment with STAT3 siRNA significantly reduced the suppression of proinflammatory brucellacidal activity but not phagolysosome fusion by rIL10. Thus, our data provide the first evidence that clearly indicates the suppressive role of IL10 on phagolysosome fusion and inflammation in response to B. abortus infection through two distinct mechanisms, STAT3-independent and-dependent pathways, respectively, in murine macrophages.</P>
Hop, Huynh Tan,Arayan, Lauren Togonon,Reyes, Alisha Wehdnesday Bernardo,Huy, Tran Xuan Ngoc,Min, WonGi,Lee, Hu Jang,Son, Jee Soo,Kim, Suk Elsevier 2017 Microbial pathogenesis Vol.113 No.-
<P><B>Abstract</B></P> <P> <I>Brucella</I> is a zoonotic pathogen that survives within macrophages; however the replicative mechanisms involved are not fully understood. We describe the isolation of sufficient <I>Brucella abortus</I> RNA from primary host cell environment using modified reported methods for RNA-seq analysis, and simultaneously characterize the transcriptional profiles of intracellular <I>B. abortus</I> and bone marrow-derived macrophages (BMM) from BALB/c mice at 24 h (replicative phase) post-infection. Our results revealed that 25.12% (801/3190) and 16.16% (515/3190) of the total <I>B. abortus</I> genes were up-regulated and down-regulated at >2-fold, respectively as compared to the free-living <I>B. abortus</I>. Among >5-fold differentially expressed genes, the up-regulated genes are mostly involved in DNA, RNA manipulations as well as protein biosynthesis and secretion while the down-regulated genes are mainly involved in energy production and metabolism. On the other hand, the host responses during <I>B. abortus</I> infection revealed that 14.01% (6071/43,346) of BMM genes were reproducibly transcribed at >5-fold during infection. Transcription of cytokines, chemokines and transcriptional factors, such as tumor necrosis factor (<I>Tnf</I>), interleukin-1α (<I>Il1α</I>), interleukin-1β (<I>Il1β</I>), interleukin-6 (<I>Il6</I>), interleukin-12 (<I>Il12</I>), chemokine C-X-C motif (<I>CXCL</I>) family, nuclear factor kappa B (<I>Nf-κb</I>), signal transducer and activator of transcription 1 (<I>Stat1</I>), that may contribute to host defense were markedly induced while transcription of various genes involved in cell proliferation and metabolism were suppressed upon <I>B. abortus</I> infection. In conclusion, these data suggest that <I>Brucella</I> modulates gene expression in hostile intracellular environment while simultaneously alters the host pathways that may lead to the pathogen's intracellular survival and infection.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The transcriptional profiles of <I>B. abortus</I> and BMM were analyzed by RNA-seq approach. </LI> <LI> The up-regulated <I>B. abortus</I> genes are involved in DNA, RNA manipulations and protein biosynthesis. </LI> <LI> The down-regulated <I>B. abortus</I> genes are involved in energy production and metabolism. </LI> <LI> The host defense genes are induced but cell proliferation and metabolism genes are suppressed in <I>B. abortus</I> infected BMM. </LI> </UL> </P>
Oxygen nonstoichiometry and electrical properties of La2–xSrxNiO4+δ (0 ≤ x ≤ 0.5)
Hop Dang-Hoang,정병규,허영우,김정주,이준형 한국세라믹학회 2020 한국세라믹학회지 Vol.57 No.4
The amount of interstitial oxygen ( δ ) in Sr-doped lanthanum nickel oxide (La 2– x Sr x NiO 4+ δ ) as a function of Sr content has been investigated by the thermogravimetry analysis (TGA) and X-ray photoelectron spectroscopy (XPS). La 2– x Sr x NiO 4+ δ (0 ≤ x ≤ 0.5) ceramics were prepared by the general solid-state reaction process and sintered in diff erent ambient of argon, air or oxygen, respectively. An increase in the amount of Sr decreased δ in La 2– x Sr x NiO 4+ δ , while the Ni 2+ /Ni 3+ ratio analyzed by Ni3p XPS decreased indicating that the oxidation state of Ni increased. Particularly when the sample was sintered in a low oxygen partial pressure condition such as in Ar ambient, the hole concentration strongly depended on the Sr content, suggesting that the hole generation seems mostly originated from Sr 2+ substitution into the La 3+ site.